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Citations for Millipore Sigma :
4251 - 4300 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in lysis buffer (50 mM Tris pH 8, 1 M NaCl, 10% v/v BugBuster (Novagen), 10 mM imidazole ...
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bioRxiv - Cancer Biology 2022Quote: LLC cells were treated with conditioned media from inflammasome-induced macrophages as indicated and 5 × 104 cells per well were seeded in top chambers of the transwell chamber (8 μM pore, 24-well plate, Millipore-Sigma) in FBS-free media with membrane inserts ...
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bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were fractionated using SE250 Mighty Small II (Hoefer Scientific Instruments) on SDS-PAGE minigels (1.5 mM thick, 8% polyacrylamide) and transferred to Immobilon-P membranes (Millipore IPVH00010) using a Bio-Rad Mini Trans-Blot (#1703930 ...
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bioRxiv - Cell Biology 2024Quote: ... Retroviral supernatant containing various C/EBP constructs was added to dKO-B cells together with 8 ug/mL polybrene (Sigma). Cells were infected by spinoculation at 2000 rpm at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... leaf pieces were transferred onto solid (0.8 % phyto agar [Duchefa]) MGI medium (full-strength MS medium containing 1.6 % glucose [Roth], 1 mg/l BA [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: ... The viral supernatant was filtered through 0.45 µm filter and added to the target cells along with 8 µg/ml polybrene (Sigma, H9268). Following one to three infections ...
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bioRxiv - Molecular Biology 2024Quote: ... protein was loaded onto a 10% or 8% SDS-PAGE gel and was transferred to Immobilon-FL PVDF membrane (IPFL00005, Millipore). The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041 ...
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bioRxiv - Developmental Biology 2024Quote: ... the Epiblast was separated from the Visceral Endoderm by 8 minutes incubation on ice in a solution of 2.5% pancreatin (Sigma, P3292) and 0.5% trypsin (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... and 25 mM Tris pH 8) and concentrated with centrifugal concentrators (10 kDa MWCO, Millipore Sigma-Aldrich, Burlington, MA, USA) to a final volume of 0.1 mL ...
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bioRxiv - Cell Biology 2024Quote: ... were resuspended at cell densities of 8×106 cells/mL for each cell type into a hydrogel comprised of 6.5mg/mL fibrin (Sigma #341573) and supplemented with 0.2mg/mL fibronectin (Sigma #F1141 ...
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bioRxiv - Cell Biology 2024Quote: ... were separated by 8 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore) at 120 V for 2 hours using transfer buffer (25 mM Tris-base ...
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bioRxiv - Genomics 2023Quote: ... cells were transduced with the GFP-pilot lentiviral supernatant supplemented with 8 μg/ml polybrene (Sigma-Aldrich TR-1003-G) at MOI ∼0.1 ...
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bioRxiv - Immunology 2024Quote: ... 1.4×108 human primary CD4+ T cells were transduced with lentivirus at a multiplicity of infection of 2 with 8 µg/ml dextran (Sigma). Virus and DynaBeads were removed after 3 days of transduction ...
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bioRxiv - Genomics 2024Quote: ... The spinfection was carried out at room temperature for 30 minutes spinning at 1000g and the cells were supplemented with 8 μg/ml polybrene (hexadimethrine bromide, Sigma). 2 µg/ml puromycin was added to the cells 24 hours post infection ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Cancer Biology 2024Quote: ... zebrafish larvae (8-9 per condition) were dissociated into single cells by applying enzymatic digestion using 5µl of liberase (Sigma-Aldrich) (2.5mg/ml ...
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bioRxiv - Biophysics 2024Quote: The purified protein was concentrated to 5–8 mg/ml using a 50 kDa molecular weight cutoff (MWCO) centrifuge concentrator (Millipore) and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2024Quote: ... Gene expression was induced at OD600 ∼0.8 by addition of either 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH) or 0.2 µM anhydrotetracycline (Sigma). Cells were grown overnight at 22°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 17 β-estradiol was added to the drinking water twice a week at a final concentration of 8 μg/mL (cat# E2758; Sigma). CHK2 activator (3 ...
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bioRxiv - Immunology 2023Quote: ... 8-10 pieces (ca. 150mg total) were placed in each cryovial containing 1mL Cryostor CS10 (Sigma Aldrich, St. Louis, MO) or 1mL 90% FBS/10% DMSO and placed in Corning CoolCell LX Cell Freezing Container (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: MIN6 cells stably expressing Cas9 were infected with this lentivirus at half volume with 8 μg/ ml polybrene (TR-1003-G, Sigma) overnight ...
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Prior to fixation we live-stained all the embryos with either Caspase-3/7 or Caspase-8 (Supplementary Table 30) for 30 min and subsequently removed the zona pellucida using Acidic Tyrode’s Solution (Sigma-Aldrich) for 30 sec – 2 min and subsequently washed in PBS/2%BSA.
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bioRxiv - Cell Biology 2023Quote: ... the reaction between peptide conjugate and crosslinkers (PEG-4VS and PEG-4 ACLT) was performed in stoichiometric ratio 1:1 in30mM HEPES buffer at pH 8 (Sigma) diluted in 1x HBSS (Gibco) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The mid log phase cells were diluted to 104 cells/ml (assuming 1 OD = 8×108 cells/ml) into LB with 0.005% Antifoam 204 (Sigma A6426) and 1 mg/ml BSA (Sigma-Aldrich A2153-100G) ...
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bioRxiv - Microbiology 2023Quote: ... supernatant containing lentivirus was filter sterilised and combined with TREx BCBL1-Rta cells in 8 μg/ml polybrene (Merck Millipore). After 8 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and plated on 35mm dishes (8×105 cortical cells and 4×105 hippocampal cells per dish) coated with poly-DL-ornithine (Sigma), in DMEM (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... the developing step took place by submerging SU-8 coated wafer into propylene glycol monomethyl ether acetate (PGMEA; Sigma-Aldrich) solution and incubating in it for 8 min with periodical agitation ...
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bioRxiv - Genomics 2022Quote: ... FAD, and SAD iPSCs and maintained in Essential 8 media (E8 media, A1517001, Thermoscientific) supplemented with ROCK inhibitor (SCM075, Millipore) for 4 days ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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bioRxiv - Immunology 2023Quote: ... USA) cells and the respective sublines were maintained in RPMI-1640 medium containing 8% fetal calf serum (FCS) (Sigma, F7524), 2 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed twice with lysis buffer and proteins were eluted from the beads with 8 M urea (U5128; Sigma) and 0.1 M Tris–HCl at pH 8.5 ...
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bioRxiv - Neuroscience 2023Quote: P5 Aldh1l1:eGFP mice were anesthetized with ketamine-xylazine (140 and 8 mg/kg, respectively, i.p.) and injected intraperitoneally with 50 µL of puromycin (P7255, Sigma Aldrich) diluted in water (10 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: 10 female frozen heads per tube were homogenised in 2% SDS buffer (Cat No. 42- 801-8 0ML, Millipore Sigma) containing 1X RIPA buffer (Cat No ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Cancer Biology 2023Quote: Non-tumor tissue was transferred into a 50 ml tube pre-filled with 25 ml dissociation/chelation buffer (8 mM EDTA, 0.5 mM DTT, Dnase I (100 units/ml, Millipore Sigma)) ...
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bioRxiv - Developmental Biology 2023Quote: ... DRG explants from E12.5 mouse embryos were dissected as described above and cultured for 18-24 hours in DRG growth medium on 8-chamber glass slides coated with 1 mg/ml poly-D-Lysine (PDL) (Sigma) and 5 μg/ml laminin (Millipore).
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bioRxiv - Molecular Biology 2023Quote: ... were subjected to SDS-PAGE with 8% or 15% Tris-Glycine gels and transferred to Immobilon- P PVDF membranes (Millipore) using a TransBlot SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate supernatant was filtered with a 0.33 µm filter unit and loaded onto a 5 mL Ni-NTA column (17528601 Cytiva) pre-equilibrated with buffer A (20 mM Na-HEPES pH 8, 300 mM NaCl, 20 mM Imidazole (I202 Sigma) pH8) ...
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bioRxiv - Bioengineering 2022Quote: ... hiPSCs were collected and centrifuged at 300 g for 5 minutes and resuspended in Essential 8 Flex medium containing 2μM ROCK inhibitor (Thiazovivin) (Millipore Sigma). hiPSCs were counted using a Moxi cell counter (Orflo Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Where indicated an RGD peptide (sequence - Acetyl-Cys-Doa-Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2; Doa = 8-amino-3,6-dioxaoctanoic acid, Millipore Sigma) was added to the cell suspension at a concentration of 0.5 mM ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... The target cells were infected overnight by filtered viral supernatant in the presence of 8 μg/mL of polybrene (#TR-1003-G, Millipore). The infected cells were selected with puromycin (2 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Boyden chamber invasion tests were conducted using Millicell with hanging Transwell polyethylene terephthalate inserts (8 µm pore size, Millipore, PIEP12R48). 200 µl of serum-free medium containing 5104 unstimulated MG63 or LM7 cells was added to the upper compartment ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown in complete medium and for days 8-14 this medium was supplemented with 50μM L-ascorbic acid (Sigma-Aldrich). Medium changes were performed every 3 days ...
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bioRxiv - Biophysics 2023Quote: ... Supernatant post-48/72h transfection was collected for transduction in target cells supplemented with 8 µg/mL polybrene (EMD Millipore). Single cell clones of Cas9 and sgKRAS-transduced cells were sorted into 96-well plates using a FACSMelody benchtop sorter (BD Biosciences ...
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bioRxiv - Genomics 2023Quote: ... and 8 µg of the plasmid of interest were mixed with 200 µl of CaCl2 (1 M, Sigma, 21115-100ML). Volume was completed with sterile water up to 800 µl ...