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Citations for Millipore Sigma :
4301 - 4350 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed by spinoculation at 1200 X g for 2 hours at 32° C in the presence of virus particles and 8 ug/ml polybrene (Millipore). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... 600,000 cells were seeded into each well of a 6-well plate and one week later were infected with up to 100ul virus per well (and 8 ug/ml polybrene (TR-1003-G, Sigma)) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 800µl NP-40 buffer (20mM Tris pH=8, 150-200mM NaCl, 0.5mM EDTA, 1% NP-40, 1mM DTT, 5% glycerol and protease inhibitor cocktail; Sigma-Aldrich). Protease inhibitor cocktail (Bimake ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 300μl extraction buffer (50mM Tris pH=8, 150mM NaCl, 0.5mM EDTA, 1% NP-40, 5%, 1mM DTT, and protease inhibitor cocktail [Sigma-Aldrich]). Cell extracts were sonicated and centrifuged at 4°C for 15 min at 16,000 ×g ...
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bioRxiv - Cell Biology 2023Quote: ... filtered and applied onto the desired HeLa cells for 24-48 h in the presence 8 mg/ml polybrene (Sigma). Cells were then recovered and expanded in growing DMEM for 5-7 days prior to sorting by FACS to match protein expression levels where possible.
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bioRxiv - Immunology 2023Quote: ... Lentiviral supernatants were used to infect Ramos cells via spinfection (2350 rpm for 90 min) in the presence of 8 μg/ml Polybrene (Sigma). Flow cytometry (BD Aria III sorter ...
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bioRxiv - Microbiology 2022Quote: ... nuclei were resuspended in one volume of RLN buffer (50 mM Tris-HCl pH 8, 140 mM NaCl, 1.5 mM Mg2Cl (Sigma-Aldrich), 10 mM EDTA pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates containing 50 µg protein were separated by electrophoresis on 8–12% polyacrylamide gels and the separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore, USA) at 200 mA for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... JCPyV-infected and mock-infected astrocytes were detached using TrypLE (GibcoTM) and replated at 30 000 cells/cm2 on 8-well Milicell EZ glass chamber slides (Millipore). Following a 24-hour incubation period ...
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bioRxiv - Neuroscience 2023Quote: ... JCPyV-infected and mock-infected astrocytes were detached using TrypLE (GibcoTM) and replated at 30 000 cells/cm2 on 8-well Milicell EZ glass chamber slides (Millipore). The next day ...
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bioRxiv - Plant Biology 2023Quote: ... 8-day-old plants (n=15) were dipped for 8 minutes in Lugol staining solution (0.1% I, 1%KI, Sigma-Aldrich) and observed in an Eclipse E200 Microscope (Nikon ...
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bioRxiv - Cell Biology 2023Quote: ... Virus particles were collected 48 h after transfection then filtered through a 0.45 μm syringe filter and used to infect MCF-10A cells in the presence of 8 μg/ml polybrene (Sigma). Clones of interest were selected using 1 μg/ml puromycin (Sigma).
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon tissues from one wild type and one Olfr78-GFP KO mice (respectively 8 and 6 months-old) were cut in small pieces and digested with 0.5 mg/ml Collagenase I (Sigma Aldrich) for 25 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted with 6x Laemmli buffer (70% v/v Tris HCl pH 6.8, 10% w/v SDS, 30% (v/v) glycerol (Carl Roth, 3783) and 0.01% w/v Bromophenol blue (Sigma, B0126) containing 10% v/v b-mercaptoethanol (Carl Roth ...
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bioRxiv - Immunology 2023Quote: ... The tissue was cut into very small pieces using a clean razor blade and transferred into 0.3 mL RIPA buffer containing 50 mM Tris-HCl pH7-8 (Sigma-Aldrich), 150 mM NaCl (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Viruses were titered and then used to transduce cells supplemented with 8 µg/ml protamine sulfate (Sigma-Aldrich, USA #P3369).
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bioRxiv - Cancer Biology 2023Quote: ... The tumor cell lines and UPCs were infected with the concentrated virus in a culture media containing 8 µg/ml Polybrene (Sigma) and positively transduced cells selected by FACS for the expression of the corresponding fluorescent reporter.
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bioRxiv - Developmental Biology 2023Quote: ... pharyngeal tissues were obtained by treating the wall of the embryonic pharynx with a solution of pancreatin (8 mg/ml, Sigma) for 30–90 min on ice ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: SNU475 cells in a well of a 24 well plate (2×104 cells/well) were treated in 500 μL complete RPMI medium with 8 μg/mL polybrene (Sigma) and 2μL (2 multiplicity of infection ...
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bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... Mafb deletion was induced in adult male mice (8-10 weeks) with two consecutive days intraperitoneal injection of Tamoxifen (SIGMA) at 40mg/kg dissolved in corn oil ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested in ice-cold 1X PBS and mechanically homogenized in 2ml 8% (w/v) sucrose (0.25M; Sigma, S9378) using glass douncers ...
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bioRxiv - Cancer Biology 2023Quote: Gastrocnemius muscle samples were lysed in lysis buffer (10% N-Deoxycholate, 0.1% SDS, 1% Triton X-100, 10 mM Tris pH 8, 140 mM NaCl, phosphatases/proteases inhibitors [Sigma-Aldrich] ...
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bioRxiv - Immunology 2023Quote: ... of Escherichia coli O111:B4 or O127:B8, and Phorbol 12-myristate 13-acetate (PMA, CAS: 16561-29-8) were obtained from Sigma. Adenosine 5’-triphosphate disodium salt (ATP ...
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bioRxiv - Genomics 2023Quote: ... per 0.1 g of ground tissue powder was resuspended in 400 µL of lysis buffer (100 mM Tris-HCl [pH 8], 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393] ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA or RNase H digested DNA were immunoprecipitated with 8 μg of S9.6 antibody (Millipore #MABE1095) overnight at 4°C in DRIP binding buffer (10 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2023Quote: ... Harvested cells were resuspended in lysis buffer (50 mM HEPES, pH 8, 200 mM NaCl, 0.5mM TCEP, 5 U/mL benzonase (Millipore Sigma), EDTA-free cOmplete protease inhibitor cocktail tablet (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Total cell lysate samples were then separated on 8-12% SDS-PAGE gels and transferred to PVDF membranes (Millipore, ISEQ00010). Membranes were blocked with TBST containing 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1-hTERT cells constitutively expressing rtTA (reverse tetracycline Trans Activator) were used as target cells) in the presence of 8 µg/mL polybrene (Millipore) for at least 6 hours (maximum 24 hours) ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrated virus equivalent to that harvested from half a 10 cm dish of HEK293T cells was added to 2 × 105 BEL-A cells in the presence of 8 μg/mL polybrene (Sigma) for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... and lysed using a solution of 4% SDS and 50 mM TRIS-HCl (pH=8) containing protease inhibitors (Sigma-Aldrich) as described previously79 ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: Cell lysis was performed using RIPA lysis buffer (50 mM Tris-HCl [Carl Roth, 9090.3], pH 8, 150 mM sodium chloride, 1% IGEPAL CA-630/NP-40 [Sigma-Aldrich, 56741] ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were grown with or without Dox (0.5 µg/ml) for 8–14 d depending on the cell line and afterwards stained with crystal violet (Sigma-Aldrich). Colony number and area was determined on scanned plates using Fiji (Image J)57,58 ...
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bioRxiv - Cancer Biology 2023Quote: Bone marrow cells were acquired by flushing the femurs and tibias of 6- to 8-week-old female BALB/c mice and treated with red blood cell lysis buffer (Sigma). Lineage negative hematopoietic stem and progenitor cells (HSPCs ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was changed to Essential 8 (E8) medium containing 100 µM sodium butyrate (NaB, Sigma-Aldrich, Cat. no. B5887). After 3 – 4 weeks ...
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bioRxiv - Genomics 2023Quote: ... per 0.1 g of ground tissue powder was resuspended in 400 µL of lysis buffer (100 mM Tris-HCl [pH 8], 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393] ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS to permeabilise and a second time for 1h in 0.5% Triton X-100 1% fish gelatin (CAS #9000-70-8, Sigma-Aldrich) in PBS to block ...
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bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Synthetic neotetraploids were created from six genetically distinct diploids using the mitotic inhibitor colchicine (Sigma Aldrich, CAS: 64-86-8). Details of the methodology are found in Anneberg et al ...
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bioRxiv - Genomics 2024Quote: ... HepG2 cells expressing dCas9-BFP-KRAB were transduced with dJR092 library lentiviral supernatant with polybrene (8 µg/mL, Sigma-Alrich) with a targeted low infection rate of ∼10% ...
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bioRxiv - Cell Biology 2024Quote: ... fibroblasts were seeded and allowed to attach overnight before incubation with the virus and polybrene (8 µg ml−1, Sigma) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transduced with the MAGE KO library (MOI < 0.5) in the presence of 8 µg/mL (final concentration) polybrene (Sigma-Aldrich). 48 h after viral transduction ...
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bioRxiv - Neuroscience 2024Quote: ... Three baseline measurements of oxygen consumption rate (OCR) were measured prior to injection of 8 μM UK5099 (Sigma-Aldrich, PZ0160), 16 μM etomoxir (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... 0.2Xconcentrated BHI media was obtained by dissolving 7.4 g while a third 1X solution was supplemented by 8 g/L of D-Glucose (Sigma Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... 8–9-week-old mice were intraperitoneally (IP) injected with 0.1 mL of 25 mg/mL tamoxifen (TAM; Sigma, T5648) in corn oil (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... coli cell pellets were resuspended in low imidazole buffer (25 mM Tris HCl pH 8, 25 mM imidazole, 1 M NaCl, Sigma FAST Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Microbiology 2024Quote: ... A hyaluronic acid solution was first generated by suspending 300 mg unfractionated HA sodium salt and 8 g of bovine serum albumin fraction V at pH 5.2 (Sigma-Aldrich) in 200 mL of water and slowly adjusting to pH 7.5 using 0.1 M sodium hydroxide ...