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Citations for Millipore Sigma :
3701 - 3750 of 4383 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... the gel was blotted and probed with antisense telomere probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) or with a 1.6 kb fragment containing the sequence (TTAGGG)n62 ...
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bioRxiv - Genomics 2019Quote: ... Debris was removed with a density gradient centrifugation using the Nuclei PURE 2M Sucrose Cushion Solution and Nuclei PURE Sucrose Cushion Buffer from Nuclei PURE Prep Isolation Kit (#NUC201-1KT, Sigma Aldrich). Nuclei PURE 2M Sucrose Cushion Solution and Nuclei PURE Sucrose Cushion Buffer were first mixed in 9:1 ratio ...
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bioRxiv - Microbiology 2019Quote: ... Ethanol was mixed with the aqueous phase and RNA isolation protocol was continued as per GenElute Mammalian Total RNA Miniprep kit (RTN350, Millipore Sigma) protocol ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted using the RNeasy Mini Kit and the RNase-Free DNase Set according to the manufacturer’s protocol (Sigma-Aldrich, Switzerland). Total RNA was reverse transcribed using all-in-one RT-PCR (BioTool ...
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bioRxiv - Physiology 2019Quote: The interactions between TRPA1 and IL-R1 in nodose ganglia cultures were detected by PLA with Duolink in situ kit (Sigma- Aldrich). Primary nodose ganglion cells were harvested and cultured on glass coverslips as described above ...
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bioRxiv - Microbiology 2021Quote: ... were fluorescently labelled by the addition of 2 μl PKH26 dye (1 mM stock concentration) from the MINI26 PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich) and mixed by pipetting ...
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bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were washed with cold PBS and centrifuged at 2000 x g for 5 minutes and stained using a Muse™ Cell Cycle Kit (Merck Millipore) for 30 minutes in dark conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Cell Biology 2021Quote: ... proximity ligation assays were performed on isolated HSCs with the Duolink in Situ Red Starter Kit Mouse/Rabbit (DUO92101, Millipore Sigma), adapting the vendor’s protocol for HSCs ...
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bioRxiv - Bioengineering 2021Quote: Total RNA was isolated from 9-day-old developing Arabidopsis siliques (Van Erp et al., 2014) using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich). The isolated total RNA was then treated with DNase I (Amplification Grade ...
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bioRxiv - Neuroscience 2021Quote: Eighteen-µm cryostat sections from the cerebrum were fixed in 4% PFA directly on slides and stained using the FragEL DNA Fragmentation Kit (QIA33; Millipore Sigma) according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and cortex was determined following the standard Ellman reaction principle using a commercially available AChE activity assay kit (Catalog no. MAK119, Sigma-Millipore). The Ellman assay [42] was used to measure ChE activity in each brain region using 5,5’-dithio-bis (2-nitrobenzoic acid ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 4 μm sections of whole lung were stained with either haematoxylin and eosin or Grocott stain (Silver stain kit, Sigma HT100A).
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bioRxiv - Plant Biology 2021Quote: The inhibitory effect of recombinant P235 on recombinant glycosyl hydrolase was assayed using the β-Glucosidase Activity Assay Kit (MAK129, Sigma). Enzymatic reactions were carried out in K-Phosphate buffer (100 mM ...
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bioRxiv - Bioengineering 2021Quote: ... 250 μL of recovered solution was transferred into a 96-well plate and the recovered glucose concentration was measured using a glucose (GO) assay kit purchased from Sigma (GAGO20). Glucose recovery rate was defined by the following formula.
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bioRxiv - Cell Biology 2021Quote: For the isolation of lysosome using ultracentrifugation method was performed following the manufacturer’s instructions (Lysosome Isolation Kit, Sigma-Aldrich, LYSISO1-1KT).
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bioRxiv - Cell Biology 2021Quote: ... alkaline phosphatase staining was completed within each well using a commercially available Leukocyte Alkaline Phosphatase staining kit (Sigma Aldrich 86R-1KT). Whole well images were collected through brightfield microscopy and quantification of colony forming units (CFUs ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from 4-day-old dark-grown seedlings plus 3hrs light treatment using the Spectrum Plant Total RNA Kit (Sigma-Aldrich). One microgram of total RNA was treated with DNase I to eliminate genomic DNA and then reverse transcribed using SuperScript III (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from the frozen tissues using Spectrum™Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Plant Biology 2020Quote: ... we extracted total RNA from pools of 6-8 shoot apical meristems (SAM) after the terminal spikelet stage from four biological replicates using the Spectrum Plant Total RNA Kit (Sigma-Aldrich). The cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 100 mg of ground-frozen samples using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) and the RNA purity was determined through NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μL of samples and standards were added to a 96-well plate and glucose was measured using the Glucose Oxidase (GO) Assay kits (Sigma, GAGO20). Total circulating sugars was measured from the trehalase-treated sample ...
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bioRxiv - Physiology 2020Quote: ... 1971), MJF465 (Levina, 1999), MJF641, and MJF516 (Edwards et al., 2012) was performed using the Novagen λDE3 Lysogenization Kit (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from 7-d-old Emmenanthe penduliflora seedlings grown in 16:8 photoperiod on moistened filter paper using Spectrum Plant Total RNA kit (Sigma-Aldrich) after removal of seed coats ...
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bioRxiv - Microbiology 2019Quote: ... and the molecular sizes of each fraction was estimated by gel filtration molecular weight markers (Kit No: MWGF1000 of Sigma-Aldrich), and each fraction was collected for protein identification by Western blot.
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bioRxiv - Cell Biology 2021Quote: ... The TUNEL assay was performed on the testis sections (5 μm) with ApopTag plus peroxidase in situ apoptosis detection kit (Millipore, S7101).
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed with 2% paraformaldehyde in PBS and proximity ligation assays (PLA) were performed with the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich) according to the manufacturer’s protocol.
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... The EV pellet was resuspended in 200 μl of filtered PBS and stained by using the PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labelling (Sigma-Aldrich). The dye solution was freshly prepared by adding 4 μl of PKH26 dye to 1 ml of Diluent C ...
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bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
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bioRxiv - Biophysics 2021Quote: CoA levels in the protein solution after proteinase K digestion were quantified using a commercial CoA assay kit according to manufacturer’s protocol (Sigma-Aldrich; MAK034). CoA concentration is determined by an enzymatic assay ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and centrifuged at 900g for 5mins and GLP-1 secretion over 1h treatment with GYP was measured using a total GLP-1 ELISA kit (Millipore, UK) according to manufacturer’s specifications.
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bioRxiv - Cell Biology 2021Quote: RAW 264.7 cells were grown and used according to manufacturer’s instructions to obtain membrane fractions by differential sedimentation ultracentrifugation using a density gradient (Lysosome Isolation Kit, Sigma-Aldrich, LYSISO1). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained for proximity ligation assay (PLA) using a Duolink® kit according to the manufacturer’s protocol (DUO92101, Millipore Sigma). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... media was removed from wells and cells were incubated with MTT from the Cell Proliferation Kit (Sigma Aldrich, catalogue no. 11465007001), and the colorimetric assay was performed following manufacturer’s directions ...
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bioRxiv - Microbiology 2022Quote: ... The fraction of proteins >1 kDa was obtained by dialysis using the Mini Dialysis Kit 1 kDa (Sigma #GE80-6483-94). All kits were applied according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Physiology 2022Quote: Determination of cellular total iron levels in magnetically-sorted RPMs was carried out using the Iron assay Kit (Sigma-Aldrich, MAK025) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: PLA was carried out on 4% paraformaldehyde-fixed PS and PSP cells using the Duolink PLA Kit (Sigma-Aldrich, DUO92101-1KT) following the manufacturer protocol ...
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bioRxiv - Systems Biology 2022Quote: ... and cDNA fragments were amplified by PCR according to the manufacturer’s protocol (GenomePlex Single Cell Whole Genome Amplification Kit, Sigma Aldrich WGA4). The amplified libraries were run on a bioanalyzer to assess quality (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Osteoclast resorbed surface (Oc.S/BS) was measured following TRAP staining with Leukocyte Acid Phosphatase TRAP Kit (Sigma Aldrich, catalog # 387A-1KT). RNA was extracted from isolated whole femur for qPCR for osteoblast (Col1a1 ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Cell Biology 2022Quote: ... ear notches were collected from mouse pups to extract genomic DNA using the RED Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich). Ear notches were incubated in the tissue preparation (25 μl/sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells of the granulocytic lineage were stained on paraffin embedded tissues with the Napthol AS-D Chloracetate (Specific Esterase, CAE) Kit (Ref: 91C-1KT, Sigma-Aldrich) according to the manufacturers protocol.
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bioRxiv - Molecular Biology 2022Quote: ... The measurement of total glutathione (tGSH) was determined (in pellets and supernatants) using the kinetic glutathione assay kit (Sigma-Aldrich, Spain) in which catalytic amounts (nmoles ...
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bioRxiv - Biochemistry 2022Quote: K562 cell line derivatives were collected by centrifugation at 1400 x g for 3 mins and nuclei were isolated using the Nuclei EZ Prep nuclei isolation kit (Sigma- Aldrich). The nuclei were fixed in 4% (v/v ...
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bioRxiv - Cell Biology 2022Quote: To quantify extracellular levels of lactate and pyruvate from iHep supernatants aliquots were taken at day 22 of differentiation and analyzed using Lactate and Pyruvate Assay Kits (Sigma-Aldrich) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... PLA was performed using the Duolink in situ PLA kit according to the manufacturer’s instructions (Millipore Sigma, DUO92002, DUO92004 and DUO92014). In brief ...
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bioRxiv - Cancer Biology 2022Quote: RAS and RAC activity were measured using a RAF-1 RBD and PAK-1 PDB pull-down assay kits respectively (Cat#17218 and Cat#14325, Millipore Sigma) following manufacturer’s instructions ...