Labshake search
Citations for Millipore Sigma :
3651 - 3700 of 4383 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Immunology 2023Quote: ... Neisserial genomic DNA was extracted from cell suspensions of overnight culture plates using the GenElute Bacterial Genomic DNA Kit (Sigma). Genomic libraries were generated using Illumina Nextera DNA Flex Library Prep according to the manufacturer’s instructions and sequenced on Illumina MiSeq 2×250bp paired end platform ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The concentration of tGSH (nM/mg of erythrocyte pellet) was assayed using a commercial kit (Sigma-Aldrich, St Louis, MO) and according to (Galván and Alonso-Alvarez ...
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bioRxiv - Microbiology 2023Quote: ... Regular testing for mycoplasma contamination was performed to confirm contamination free culture using a PCR-detection kit (Sigma-Aldrich/Merck). The details of cell lines are provided in key resources table ...
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bioRxiv - Neuroscience 2023Quote: ... all cultures were monthly tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma, cat. no MP0035-1KT).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were tested frequently for mycoplasma contamination using LookOut® Mycoplasma PCR Detection Kit (cat no: MP0035-1KT, Millipore sigma). CAOV3 ...
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bioRxiv - Plant Biology 2024Quote: Seedlings were harvested in liquid nitrogen and total RNA was extracted using a Spectrum Plant Total RNA kit (Sigma-Aldrich). The sequencing was made on an Illumina HiSeq 2500 system using 100-bp single-end reads protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Unique integration of the cassette in the mutants was then controlled by Southern blot using DIG-High Prime DNA Labelling and Detection Starter Kit I (Sigma). Primers used for these experiments are listed in Table S1 ...
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bioRxiv - Plant Biology 2024Quote: All samples were homogenised using Geno/Grinder 2010 (SPEX SamplePrep) and total RNA was isolated from homogenised samples using the Sigma Spectrum Plant Total RNA kit (Sigma) supplemented with the On-Column DNase I Digestion step to remove genomic DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The total ATP production was measure in nm/µg of protein by the ATP colorimetric assay kit (Sigma, MAK190-1KT) and experiment was performed as per provided manufacturer’s procedure ...
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bioRxiv - Molecular Biology 2024Quote: ... and a random RT-PCR amplification was performed using the TransPlex® Complete Whole Transcriptom Amplification Kit WTA2 (Merck Millipore) following the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of sialic acid in the liposomes was determined using a Sialic Acid Assay Kit (MAK314-1KT, Sigma Aldrich), and the concentration of liposomes containing a ganglioside was adjusted to the same level for use in microscopic observation.
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... kidney and gonads where available and from adult testis and ovaries with the GenElute Mammalian Total RNA Miniprep Kit (Sigma) and DNase treated using DNA-free (Ambion ...
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bioRxiv - Biochemistry 2024Quote: ... 400 μg total RNA in 250 μl water was applied to a GeneElute mRNA miniprep kit (Sigma-Aldrich; MRN70-1KT), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolation of nuclear proteins was achieved with the CelLytic™ NuCLEAR™ extraction kit (Sigma Aldrich, St. Louis, MO) using the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... DNA was extracted from the euthanized embryos using Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... tissues were homogenized in 150 μL of ATP Assay Buffer from the ATP Colorimetric/Fluorimetric Assay Kit (Sigma-Aldrich, MAK190). For protein extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... profiling at Broad between January and December 2018 and were routinely tested for mycoplasma using LookOut Mycoplasma PCR Kit (Sigma)
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bioRxiv - Microbiology 2024Quote: ... The plasmid containing the desired complement was extracted from the confirmed colonies using GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). The plasmid containing the required complement was then electroporated into the phage-resistant mutants ...
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bioRxiv - Molecular Biology 2024Quote: ... Mycoplasma contamination was tested to confirm contamination-free cell culture using a PCR-detection kit (Sigma-Aldrich, cat no# MP0035).
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bioRxiv - Microbiology 2024Quote: The effect of GpsB on the inorganic phosphate released by FtsZ and truncated FtsZ was examined using malachite green phosphate assay kit (Sigma). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA from control and treated samples was extracted after Sirodesmin PL treatment using a Plant Spectrum Total RNA Kit (Sigma), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Approximately 100 milligrams of tissue from either in vitro-grown plantlets or leaf tissue from greenhouse-grown plants was used for genomic DNA extraction (GenElute™ Plant Genomic DNA Miniprep Kit, Sigma). PCR was performed using primers SWEET10a_g-F and SWEET10aPro-R (Table S1) ...
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bioRxiv - Plant Biology 2020Quote: ... which was extracted from the pool of six biological replicates of both control and salt treated plants by using Sigma spectrumTM Total RNA kit (Sigma, USA) and cDNA synthesis was performed by using the PrimeScriptTM 1st strand cDNA synthesis kit (TAKARA ...
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bioRxiv - Cancer Biology 2021Quote: To detect apoptosis in the cells after bergamottin treatment In Situ cell death detection kit from Sigma-Aldrich ((St. Louis, MO) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were snap frozen in liquid nitrogen and nuclei were extracted from cell pellets (Sigma Nuclei Isolation Kit #NUC-101). Chromatin was fragmented in sonication buffer (50mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit, Millipore-Sigma). Cell proliferation was evaluated by Ki-67 immunostaining (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: Periodic acid-Schiff (PAS) staining was performed to stain mucin-producing goblet cells in murine EDMs using a PAS Kit (Sigma-Aldrich) as described previously [8] ...
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bioRxiv - Immunology 2021Quote: ... ROS release was monitored for three hours with measurements every two minutes using a fluorometric hydrogen peroxide assay kit (Sigma-Aldrich) and a Glomax microplate reader (Promega ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: Egg white immunoglobulins (300 μl) were purified using a commercially available protein A affinity chromatography kit (PURE-1A, Sigma Aldrich Co.). The manufacturer’s instructions were followed ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines used in this study were negative for mycoplasma contamination as determined with Lookout Mycoplasma PCR Detection Kit (Millipore Sigma). During the course of this study ...
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bioRxiv - Evolutionary Biology 2020Quote: ... GMA24F1A cells were cultured in 150 mm dishes and harvested for immunoprecipitation using the EZ-Magma ChIP HiSens kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and plasma was immediately separated from peripheral blood mononuclear cells and polymorphonuclear leukocytes using a dual density gradient separation kit (Histopaque 1119/1077, Sigma-Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... or black dye to allow three independent observers to determine identity while watching the tank and in video recordings (Tissue Marking Dye Kit, MDT100, Sigma-Aldrich). Females were also labeled using the same method ...
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bioRxiv - Neuroscience 2021Quote: ... were obtained using terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay (82) (based on in Situ Cell Death Detection Kit, 11684795910, red fluorescence; Sigma-Aldrich) on sections derived from rd1 and C3H retinal explant cultures ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial NAD levels were quantified from three independent mitochondrial preparations from both WT and xrn1-H41A strains using NAD/NADH Quantitation Kit (Sigma-Aldrich). Mitochondrial extract corresponding to10 μg of protein from both WT and xrn1-H41A were used to determine NAD levels as per instructions provided in the kit.
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was recovered from 150 ml of this overnight culture using the GenElute™ HP Plasmid Midiprep Kit (Sigma-Aldrich) as per the manufacturer’s instructions except for a modified elution step ...
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bioRxiv - Molecular Biology 2020Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich). A549 cells (ATCC CCL-185 ...
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bioRxiv - Molecular Biology 2020Quote: One third of samples from the IPMS experiment was loaded to a 4-12% SDS PAGE and the silver staining was carried out with ProteoSilver™ Plus Silver Stain Kit (Sigma). Each visible band was cut for trypsin digestion and MS identification ...
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bioRxiv - Molecular Biology 2021Quote: ... PLA was performed according to the manufacturer’s instructions using the following kits and reagents: Duolink in-situ PLA Probe Anti-Rabbit PLUS (Sigma-Aldrich # DUO92002), Duolink in-situ PLA Probe Anti-Mouse MINUS (Sigma-Aldrich # DUO92004) ...
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bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
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bioRxiv - Microbiology 2019Quote: ... DNA was prepared from 400 μl of each saliva sample using the GenElute ™ Bacterial Genomic DNA Kit (Sigma Aldrich, Sweden) [7].
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bioRxiv - Cancer Biology 2019Quote: ... The membrane was UV-crosslinked for 3 minutes/each side and hybridized with probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) and developed as described62 ...