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Citations for Millipore Sigma :
3601 - 3650 of 4383 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... free phosphate release in solution was analyzed by malachite green based colorimetric assay using a Phosphate assay kit (Sigma-Aldrich) by following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: The serum alanine aminotransferase (ALT) activity was analysed using a commercially available kit according to the manufacturer’s guidance (Sigma-Aldrich) and a Spark multimode microplate reader (Tecan ...
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bioRxiv - Immunology 2023Quote: ... the proteins of the parietal-cell cytosol and membrane/organelle fractions were extracted with the ProteoExtract Subcellular Proteome Extraction Kit (Millipore) to exclude the nucleic and cytoskeletal fractions ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA was extracted from three to four replicates per plant and stage using the Spectrum Plant Total RNA kit (SIGMA). MagNA Lyser Green Beads (Roche ...
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bioRxiv - Physiology 2023Quote: Free fatty acid (FFA) content was quantified in mouse plasma samples using the Free Fatty Acid Quantitation Kit (Sigma, #MAK0044) in line with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... were allowed for growth in 6-cm cell culture plates for 48 h and subjected to isolation of the cytoplasmic and nuclear fractions by using a relevant separation kit (N-3408-200ML, Sigma). During this procedure ...
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bioRxiv - Immunology 2023Quote: ... and aspartate aminotransferase (AST) concentrations were measured using an AST and ALT Activity Assay Kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Physiology 2023Quote: ... GLP-1 content in the supernatant of T0 and T20 samples was assessed using a Glucagon-Like Peptide-1 (GLP-1) Total ELISA KIT (96-Well Plate Cat. # EZGLP1T-36K, EZGLP1T-36BK, Millipore).
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bioRxiv - Plant Biology 2023Quote: ... bacteria were harvested by centrifugation and pellets were resuspended in 5 ml of binding buffer (His-bind buffer kit (Millipore), proteins were extracted using a Constant cell disrupter system (Constant Systems ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were quantified by isolating the genomic DNA from 1ml of inoculate using GenElute Bacterial Genomic DNA kit (Sigma) and measuring the genomic equivalents (GE ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed at 4 days in vitro (DIV4) with 4% PFA and the Duolink PLA kit was employed as previously described 7 following the manufacturer’s (Sigma-Aldrich) protocol 45.
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bioRxiv - Biochemistry 2023Quote: ... A standard cell-free reaction (2-3 ml of RM) was carried out in 50 ml tubes using a Pur-A-Lyzer Maxi dialysis kit (#PURX35050, Sigma). The TrkBtm was expressed as a precipitate ...
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bioRxiv - Cell Biology 2023Quote: ER was purified according to the protocol optimised by combining the traditional microsome-based density gradient isolation method (Endoplasmic Reticulum Isolation Kit developed by Sigma) with the cell surface biotinylation reaction method (developed and optimised by Pierce) ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were further treated in a 96-well plate following the “Coenzyme A Assay Kit” protocol from Sigma-Aldrich. The absorbance of the colorimetric product (570 nm ...
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bioRxiv - Biochemistry 2023Quote: Apoptotic cell death was detected using the The ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit (Sigma, S7111, supplemental methods).
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma contamination of cells was tested and ruled out with the LookOut Mycoplasma Detection kit (Sigma-Aldrich; catalog number: MP0035).
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bioRxiv - Bioengineering 2022Quote: ... The cell line was tested as being negative for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) and Jumpstart™ Taq DNA Polymerase (Sigma-Aldrich).
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bioRxiv - Bioengineering 2023Quote: ... The phosphate concentration was then measured according to the manufacturer’s instructions (Malachite Green Phosphate Assay Kit, Sigma-Aldrich, The Netherlands). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... growth and pattern of metastasis in vivo and routinely screened for Mycoplasma (LookOut® Mycoplasma PCR Detection kit, Sigma-Aldrich). None of the cells used are listed in the International Cell Line Authentication Committee (ICLAC ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were stained either for ALP activity using an ALP detection kit according to the manufacturer’s instructions (Sigma-Aldrich) or with crystal violet ...
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bioRxiv - Cell Biology 2023Quote: ... NPCs were differentiated to astrocytes using the Stem Cell Technologies STEMdiff astrocyte differentiation and maturation kits during which they were split weekly with Accutase (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Tissue was then blocked as per the manufacturers conditions before proceeding with the assay using the Duolink In situ proximity ligation assay PLA kit (Sigma). Antibodies used for PLA ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Ratios between the oxidized form NAD+ and the reduced form NADH were measured using NAD+/NADH quantification kit (MAK037, Sigma) with whole cell lysates of wild type S ...
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bioRxiv - Genomics 2023Quote: ... The material was ground with mortar and pestle and the RNA was extracted with the Spectrum Plant Total RNA kit (Sigma) and treated with DNAse I amplification grade (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... immunoprecipitated NS from the four S-phase fractions collected by flow cytometry or from an asynchronous cell population were amplified by whole-genome amplification (GenomePlex Complete Whole Genome Amplification kit #WGA2; Sigma) according to the manufacturer’s recommendations to obtain sufficient DNA amount ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Genomics 2022Quote: ... Genome extraction was then performed using the GenElute Bacterial Genomic DNA (gDNA) extraction kit (Sigma-Aldrich, St. Louis, MO, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the supernatant was collected and used for ATP assay using an adenosine 5-triphosphate (ATP) bioluminescent assay kit (Sigma-Aldrich). The generated bioluminescence signal was measured with a SpectraMax M5 plate reader (Molecular Devices).
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bioRxiv - Cell Biology 2023Quote: The proximal ligation assay (PLA) was performed using the Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92103; Sigma-Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Genomics 2023Quote: ... The bisulfite-treated DNA fragments were ligated with a nine-nucleotide adapter and amplified using a GenomePlex Single Cell Whole Genome Amplification Kit (Sigma) for three rounds ...
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bioRxiv - Bioengineering 2023Quote: ... (Shrock, Shrock, and Elledge 2022) The ligated inserts were packaged with the T7Select packaging kit (Millipore Sigma, St. Louis, MO) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... 1.5µl of haemolymph was used to measure the glucose and trehalose according to the Glucose assay kit (Sigma-Aldrich #GAGO20). To remove cells and tissue debris ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... The quantity of extracellular vesicles was evaluated by measuring the protein concentrations that were measured using Bicinchoninic Acid Kit (Sigma). Cardiomyocytes or spheroids were treated with pelleted vesicles corresponding to 0.1 mg of proteins per 25 000 cells every two days from day 10 until day 24 after the initiation of differentiation.
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... and determination of the released glycerol in 10 µl of the upper phase with a commercial Glycerol Assay Kit according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2024Quote: ... run on a 2.5 % (w/v) agarose gel at 100 V for 2 h and purified using a gel extraction kit (Sigma-Aldrich). The products were then used as templates for a second PCR with distinct combinations of barcoded primers (Supplementary Table 10 ...
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bioRxiv - Neuroscience 2024Quote: ... Acetyl Coenzyme A (CoA) levels in the brain tissues were analysed using Acetyl-Coenzyme Assay Kit (Sigma-Aldrich, #MAK039-1KT) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Glycerolipid content of male and female whole bodies and female ovaries and fat bodies was measured 12 days after dsGFP and dsXPO1 microinjection using the colorimetric Serum Triglyceride Determination kit (Sigma, Triglyceride Reagent T2449 ...
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted from a 500 mg crushed root sample using GenEluteTM Soil DNA Isolation kit (Sigma-Aldrich, Canada) with slight modifications ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: The subtype of the mAb was IgG1 as determined using a subtype identification kit (Sigma-Aldrich, St. Louis, MO, USA). Non-immunoglobins in the ascites were removed by precipitation with caprylic acid ...
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bioRxiv - Neuroscience 2023Quote: ... Acyl-ghrelin concentrations in mouse plasma samples were determined by commercial ELISA kits (EMD Millipore, Billerica, MD; Catalog #EZRGRA-90K) (91).
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bioRxiv - Cancer Biology 2023Quote: The effect of Cul9 on GC cell proliferation was assessed by EdU assay using an In Vivo EdU Click kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... The amount of glucose remaining at each time point was measured by using a glucose-oxidase-peroxidase reaction kit (Sigma). Glucose phosphorylation activity was evaluated from the total amount of glucose consumed per unit of time.