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Citations for Millipore Sigma :
3551 - 3600 of 4383 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The effect of GpsB on the GTPase activity of FtsZ and FtsZΔC6 was determined by measuring the free phosphate released using the malachite green phosphate assay kit (Sigma Aldrich). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... Sizes were only referenced according to the Gel Filtration Makers Kit (for protein molecular weight 29 kDa to 700 kDa, Sigma).
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bioRxiv - Physiology 2022Quote: ... was measured on tissue homogenates and isolated CMs with a kit purchased from Sigma-Aldrich (MAK193; St. Louis, MO, USA). In brief ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed using an Apop Tag Plus Peroxidase In Situ Apoptosis Kit (Merck Millipore) following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, #17-700) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from each tissue using the Spectrum Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO, USA) with 2% PVP40 added to the extraction buffer to sequester phenolic inhibitors ...
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bioRxiv - Physiology 2023Quote: ... a colorimetric-based enzyme assay was used to measure alanine aminotransferase (ALT) levels in peripheral plasma collected from the submandibular vein monthly using a Mouse ALT Activity Assay Kit (SIGMA). Serum LBP level was measured by a Mouse Lipopolysaccharide Binding Protein ELISA Kit (HyCult Biotechnology ...
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bioRxiv - Physiology 2022Quote: ... Mitochondrial content was measured using approximately 5-10 mg of tissue with a colorimetric citrate synthase activity kit (Sigma MAK193).
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Mycoplasma testing was performed monthly for all the lines included in this study at any differentiation stage (LookOut Mycoplasma PCR Detection Kit, Sigma). All cells used in this study were consistently negative.
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bioRxiv - Physiology 2022Quote: ... frozen heart tissues were collected in Precellys® Tissue Homogenizing Mixed Beads Kit (2.0 mL) and homogenized using a PRECELLYS homogenizer (Bertin) containing ice-cold RIPA lysis buffer (R0278, Sigma-Aldrich), supplemented with protease inhibitor mix (cOmplete ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 2% paraformaldehyde for 10 min at room temperature and stained with the Hemacolor Rapid Staining Kit (Merck Millipore). Images were collected on BX61 upright microscope (Olympus ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were confirmed negative for mycoplasma approximately monthly using a LookOut Mycoplasma PCR detection kit (Sigma Aldrich, St Louis, MO).
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Microbiology 2022Quote: ... free fatty acid (C8 and longer) and glycerol concentrations were assessed using commercially available colorimetric kits (MAK044 and MAK117, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: All cell culture experiments were regularly tested for mycoplasma contamination using LOOKOUT® Mycoplasma PCR Detection Kit (Sigma-Aldrich, USA) for mesothelioma cell culture and PCR Mycoplasma Test Kit I/C (Promokine ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were authenticated by STR analysis (Suzhou, China) and tested for negative mycoplasma contamination using Mycoplasma Detection Kit (Sigma). MDA-MB-231 ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-PP1α antibody (2 μg) was added to the supplied spin column (Catch and Release Immunoprecipitation Kit; cat no. 17-500; Millipore) along with the cell lysates (500 mg ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA from sucrose gradient fractions with heparin (55 u/ml) were extracted with the RNA Purification kit (Sigma RNB100-50RXN). Northern blots were performed as described elsewhere45 ...
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bioRxiv - Cell Biology 2022Quote: NALM6 target cells were labelled with CellVue membrane dye (CellVue Claret Far Red Fluorescent Cell Linker Kit protocol; Sigma-Aldrich) and 6.25 × 104 or 1.25 × 105 cytotoxic CD8+ T cells were used at indicated effector to target rations ...
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bioRxiv - Plant Biology 2024Quote: ... Seedlings were flash-frozen in liquid nitrogen before total RNA was extracted using SpectrumTM Plant Total RNA Kit (SIGMA Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K ...
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bioRxiv - Biochemistry 2024Quote: ... Cell viability under 42 h compound treatment was determined via the Cell Counting Kit-8 (CCK-8, Sigma-Aldrich #96992) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted from single colonies after culturing in TYG using the GenElute™ Bacterial Genomic DNA kit (Sigma) and screened for tag insertions by PCR using primers flanking various att sites ...
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The remaining iron nitrate salt was allowed to cool then quantified using a colorimetric iron assay kit with 3 technical replicates for each cell type (MAK025, Sigma). The total iron from 3 technical replicates was averaged then divided by the number of cells to obtain calculated pg Fe/cell.
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bioRxiv - Physiology 2024Quote: Collagen content was analyzed by determination of hydroxyproline levels in left ventricles or in cell homogenates using the Hydroxyproline Assay kit (MAK008, Sigma) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ELISAs were used to confirm a subset of cytokines identified in the antibody arrays (CCL2, CX3CL1, and Galectin-3 rat ELISA kits, Sigma) using apical or basal supernatants from individual Transwell® inserts from the same experiment than the multiplex array.
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Neuroscience 2024Quote: Serum insulin and leptin were measured by an enzyme-linked immunosorbent assay (ELISA) kit (#EZRMI-13K and #EZML-82K; Millipore). Serum triglyceride levels and total cholesterol were measured using a commercial colorimetric assay kit (LaborLab® ...
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bioRxiv - Neuroscience 2024Quote: ... Free and total cholesterol levels were determined in sciatic nerves methanolic extracts by cholesterol quantitation kit (MAK043–1KT–Sigma Aldrich) following manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... and plasmid DNA was purified from the resulting cell pellet using a GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich #NA0310) following the manufacturer’s recommended protocol with elution in 3 mL H2O ...
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bioRxiv - Developmental Biology 2024Quote: Total ATP content in a pool of 5 oocytes was determined using the Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit (FLASC, Sigma) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Infected erythrocytes were labeled with a membrane dye using the PKH26R Red Fluorescent Cell Linker Midi Kit (Sigma #MIDI26-1KT) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Lipolysis was assessed from the release of FFA and glycerol in the secretion samples using non-esterified fatty acids measurement kit (FUJIFILM Wako Chemicals Europe GmbH) and free glycerol reagents (G7793 and F6428, Sigma) respectively and normalised to total protein contents.
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Genomics 2023Quote: ... Cells were validated by STR analysis and regularly confirmed to be mycoplasma free by Venor GeM Mycoplasma Detection Kit (Milipore Sigma). 293T cells were cultured at 37 C ...
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: RNA used for ONT sequencing was poly(A+) selected in two consecutive rounds using oligo(dT) beads (GenElute mRNA Miniprep Kit, Sigma MRN10 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed 72 h after transfection in -20°C methanol and subjected to PLA using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... After 24-hours BrdU was added to the media and 16h later cell proliferation was evaluated using the BrdU Cell Proliferation ELISA kit (EMD-Millipore). Colorimetric analysis was done with single channel 450nm intensity ...
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bioRxiv - Immunology 2023Quote: ... 3×106 cells were activated for 3h and lysates were obtained as described in the Technical Bulletin accompanying the Aconitase Assay Kit from Sigma. Aconitase activity in the lysates is determined per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was added to the reaction mixture along with 10 μL of (NH4)Fe(SO4)2/cysteine solution (Aconitase Assay Kit, Sigma). The mixture was incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and tumor necrosis factor α (TNFα)) was performed using the Milliplex Mouse Cytokine Magnetic Kit (EMD Millipore, Cat # MCYTOMAG-70K) and Luminex MAGPIX instrument at the Vanderbilt Hormone Assay and Analytical Service Core ...
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bioRxiv - Cancer Biology 2023Quote: RIP assay was performed following the manufacturer’s instructions (EZ-Magna RIP RNA-binding Protein Immunoprecipitation Kit, Sigma-Aldrich, #17-701). Antibody against eIF4G was used for the part of immunoprecipitation ...
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bioRxiv - Genomics 2023Quote: To obtain sufficient nuclei to run the same suspension across both fixed kits we used EZ lysis buffer (Sigma NUC101), at the expense of greater debris ...
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed using the Duolink® In Situ Red Kit Mouse/Rabbit (Sigma-Aldrich, Cat # DUO92101). For in vitro PLA ...
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bioRxiv - Cell Biology 2023Quote: ... histone H1.2 knockout heterozygous (Het) and homozygous (KO) mice are isolated using the Nuclei Isolation ‘Kit Nuclei EZ Prep’ (NUC101, Sigma). Then the nuclei are treated with 500 µM MNNG with fresh-made NAD+ (N0632 ...
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bioRxiv - Biochemistry 2023Quote: ... acetyl-CoA was quantified in a 96-well microplate format by a commercial fluorescence assay kit (MAK039, Sigma Aldrich, USA) according to the supplier’s instructions ...