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Citations for Millipore Sigma :
3651 - 3700 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Mafb deletion was induced in adult male mice (8-10 weeks) with two consecutive days intraperitoneal injection of Tamoxifen (SIGMA) at 40mg/kg dissolved in corn oil ...
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bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested in ice-cold 1X PBS and mechanically homogenized in 2ml 8% (w/v) sucrose (0.25M; Sigma, S9378) using glass douncers ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS to permeabilise and a second time for 1h in 0.5% Triton X-100 1% fish gelatin (CAS #9000-70-8, Sigma-Aldrich) in PBS to block ...
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bioRxiv - Biophysics 2024Quote: ... Gene expression was induced at OD600 ∼0.8 by addition of either 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH) or 0.2 µM anhydrotetracycline (Sigma). Cells were grown overnight at 22°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 17 β-estradiol was added to the drinking water twice a week at a final concentration of 8 μg/mL (cat# E2758; Sigma). CHK2 activator (3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were grown with or without Dox (0.5 µg/ml) for 8–14 d depending on the cell line and afterwards stained with crystal violet (Sigma-Aldrich). Colony number and area was determined on scanned plates using Fiji (Image J)57,58 ...
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bioRxiv - Cancer Biology 2023Quote: Bone marrow cells were acquired by flushing the femurs and tibias of 6- to 8-week-old female BALB/c mice and treated with red blood cell lysis buffer (Sigma). Lineage negative hematopoietic stem and progenitor cells (HSPCs ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was changed to Essential 8 (E8) medium containing 100 µM sodium butyrate (NaB, Sigma-Aldrich, Cat. no. B5887). After 3 – 4 weeks ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Synthetic neotetraploids were created from six genetically distinct diploids using the mitotic inhibitor colchicine (Sigma Aldrich, CAS: 64-86-8). Details of the methodology are found in Anneberg et al ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were fractionated using SE250 Mighty Small II (Hoefer Scientific Instruments) on SDS-PAGE minigels (1.5 mM thick, 8% polyacrylamide) and transferred to Immobilon-P membranes (Millipore IPVH00010) using a Bio-Rad Mini Trans-Blot (#1703930 ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... supernatant containing replication-incompetent lentiviruses was filtered and applied for 24 h on the target cells in a medium containing polybrene 8 μg/mL (Sigma) (78 ...
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bioRxiv - Immunology 2024Quote: Proteins were extracted in Tris buffer (Tris 20 mM pH 8, NaCl 10 mM, MgCl2 1.5 mM) 1% Triton X-100 (Sigma-Aldrich), supplemented with 1 tablet of protease inhibitors (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The concentrated retrovirus was added to 24 h-activated T cells with 8 ng/mL polybrene (#TR-1003, Sigma-Aldrich), 200 U/mL IL-2 (#212-12 ...
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bioRxiv - Cell Biology 2024Quote: ... Arteries were then stimulated with increasing concentrations of serotonin (5-HT, from 10–8 to 3·10–5 M, Sigma), endothelium-dependent vasodilator acetylcholine (ACh ...
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bioRxiv - Genomics 2024Quote: ... HepG2 cells expressing dCas9-BFP-KRAB were transduced with dJR092 library lentiviral supernatant with polybrene (8 µg/mL, Sigma-Alrich) with a targeted low infection rate of ∼10% ...
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bioRxiv - Biophysics 2024Quote: ... 0.2Xconcentrated BHI media was obtained by dissolving 7.4 g while a third 1X solution was supplemented by 8 g/L of D-Glucose (Sigma Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... 8–9-week-old mice were intraperitoneally (IP) injected with 0.1 mL of 25 mg/mL tamoxifen (TAM; Sigma, T5648) in corn oil (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... fibroblasts were seeded and allowed to attach overnight before incubation with the virus and polybrene (8 µg ml−1, Sigma) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transduced with the MAGE KO library (MOI < 0.5) in the presence of 8 µg/mL (final concentration) polybrene (Sigma-Aldrich). 48 h after viral transduction ...
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bioRxiv - Neuroscience 2024Quote: ... Three baseline measurements of oxygen consumption rate (OCR) were measured prior to injection of 8 μM UK5099 (Sigma-Aldrich, PZ0160), 16 μM etomoxir (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... coli cell pellets were resuspended in low imidazole buffer (25 mM Tris HCl pH 8, 25 mM imidazole, 1 M NaCl, Sigma FAST Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Microbiology 2024Quote: ... A hyaluronic acid solution was first generated by suspending 300 mg unfractionated HA sodium salt and 8 g of bovine serum albumin fraction V at pH 5.2 (Sigma-Aldrich) in 200 mL of water and slowly adjusting to pH 7.5 using 0.1 M sodium hydroxide ...
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bioRxiv - Microbiology 2024Quote: ... Blood from infected mice exhibiting 8-10% gametocytaemia was added to ookinete medium (20% FBS, 100 μM xanthurenic acid (Sigma), 24mM sodium bicarbonate ...
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bioRxiv - Cancer Biology 2020Quote: Tetracycline-inducible CALM-AF10 mouse hematopoietic progenitor cells were cultured in 15% FBS DMEM medium without IL-6 and mCSF and treated with either 4 µg/mL doxycycline (DOX) (Millipore Sigma, Billerica, MA) or DMSO for 3 Days ...
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bioRxiv - Immunology 2020Quote: ... 100uL per well of culture medium was replaced with 100uL fresh complete media supplemented with 50U/mL IL-2 (Millipore Sigma, Cat # 11147528001) on days 1,3 and 5 unless otherwise stated ...
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bioRxiv - Microbiology 2022Quote: ... and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2; Catalog no. H7041; Sigma, St. Louis, MO). All the cells were incubated at 37°C in the presence of 5% CO2.
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bioRxiv - Cancer Biology 2021Quote: ... stained with a 1:40 dilution of anti-human IgG-FITC (Sigma, Mendota Heights, MN) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... ii) washed in PBS and resuspended by rotating with 6 mg human IgG (Sigma, I4506) for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...
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bioRxiv - Immunology 2021Quote: ... An additional plate was coated with goat anti-human IgG (Sigma, cat. no. I2136-1ML) at 4 μg/ml and IgM (Sigma ...
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bioRxiv - Immunology 2021Quote: Small interfering RNA (siRNA) to human 3’ UTR PKC-θ was purchased from Sigma Aldrich. YTS-2DL1 cells were transfected with siRNA specific for the indicated genes ...
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bioRxiv - Cell Biology 2020Quote: Human HeLa and HEK293T cell lines (obtained from ATCC) were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (heat-inactivated ...
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bioRxiv - Molecular Biology 2021Quote: ... 2Dorganoids were seeded in chambers pre-coated with 50 μg/ml human collagen IV (Millipore) for 2 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170, Sigma-Aldrich, USA) respectively ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) in 1X PBS 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured intact mouse or human islets were resuspended in 0.1mmol/L EGTA (Sigma-Adlrich, 234626) in serum free CRMLfollowed by transduction of indicated adenoviral constructs at 10,000 plaque-forming unit per islet equivalent (pfu/IEQ ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoblot was then incubated with HRP conjugated-goat anti-human IgG secondary antibodies (Millipore, AP309P) in a dilution of 1:10,000 for 1 h ...
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bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for human samples with PAN-Cytokeratin-PE (Sigma-Aldrich, Cat. No. SAB4700668), Vimentin-Alexa Fluor 674 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: Human Embryonic 293T (HEK293T) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum and 1% Penicillin-Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... growth media was replaced with media containing 50ng/ml BMP-12 (recombinant human, Sigma Aldrich) and 10ng/ml IGF-1 (recombinant human ...
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bioRxiv - Synthetic Biology 2019Quote: HeLa human epithelial cells were recovered from freezer stocks in 10% dimethyl sulfoxide (DMSO, Sigma) and full growth media consisting of Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Immunology 2020Quote: ... blocked with 100 μl/well of 1% human serum albumin (HAS) in PBST (Sigma-Aldrich) for 1 h at RT and then washed 4 times with 100 μl/well of PBST ...
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bioRxiv - Cell Biology 2020Quote: ... Semaphorin 6B cytoplasmic tail (aa 616-1021) (SEMA6B, amplified from human brain cDNA library, Novagen); Semaphorin 6D cytoplasmic tail (aa 684-1073 ...
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bioRxiv - Developmental Biology 2019Quote: ... mice received a second IP injection with 5 units of human chorionic gonadotropin (HCG) (Sigma). Immediately after injection females were placed into the cages of stud males ...
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bioRxiv - Cancer Biology 2020Quote: ... SCC-9 and SCC-4 (human tongue OSCC) cell lines were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...