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Citations for Millipore Sigma :
3451 - 3500 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were transduced with the sgRNA library lentivirus at 0.3 infectious units/cell diluted in 8 μg/ml polybrene (EMD Millipore) as previously described [36] ...
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bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 6-well plates before addition of fresh medium containing viral supernatant (~5×105 transducing units) and Polybrene (8 μg/mL; Sigma) and ‘spin-oculated’ for 90 min at 1,500 rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... Cancer cells were grown in 6-well plates for 24 hours and transduced with 500 μL of lentivirus aliquot diluted in cell-specific medium in the presence of 8 μg/mL of Sequebrene (Sigma) and incubated at 37°C and 5% CO2 for 72 hrs ...
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bioRxiv - Cell Biology 2021Quote: ... Click-it chemistry-based azide coupling was performed by incubation for 1 hour with 60 µM Atto594 Azide (Attotec, Germany) in 50 mM Tris buffer (pH 8) with 4 mM CuSO4 (Sigma), and 10 mM freshly prepared ascorbic acid (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... The 8 well dishes were plasma activated for 30 seconds and coated with either 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) in PBS or 20 μg/ml laminin (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... The 8 well dishes were plasma activated for 30 seconds and coated with either 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) in PBS or 20 μg/ml laminin (Merck Millipore ...
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bioRxiv - Cell Biology 2021Quote: NCMs were seeded at a density 4 or 8 × 105 cells/well in 6-well plates coated with 0.01% of collagen (Sigma-Aldrich) and cultured in a medium containing DMEM/Medium199 (4:1) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μl of each fraction was spotted on to a 10 x 8 cm piece of TLC PEI Cellulose F paper (Millipore) 1 cm above the bottom of the paper and developed for 10 mins using 400 mM NaH2PO4 pH 3.6 as the solvent ...
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bioRxiv - Bioengineering 2022Quote: ... HeLa cells were seeded at 25% confluency in 96 well plates and transduced with virus (lentiCRISPR-v2 with or without HPRT1 guide) and 8 μg/mL polybrene (Millipore). Virus was removed the next day and 2.5 μg/mL puromycin was added to remove cells that did not receive virus two days later ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cell pellet was resuspended in 1 mL TM2+PI (10mM Tris pH 8, 2mM MgCl2 + Protease Inhibitor, Sigma 11836170001) and chilled in ice water for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... 70,000-100,000 LECs/well were plated on Matrigel-coated 48-well-plates or Millicell EZ 8-well glass slides (Millipore, LabTek). LECs were allowed to adhere and form tubes for 4-6 hours before medium was aspirated and small intestinal crypts or single cells from organoids were plated atop in 20µl Matrigel domes mixed with organoid media.
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 1 × 105 Cas9 TNG MKOS MEFs were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: Purified full-length E-cadherin was concentrated to 8-10 μM and mixed with the nanodisc scaffolding protein MSP1D1 (Sigma) at a 5 fold molar excess ...
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bioRxiv - Cell Biology 2022Quote: ... Viruses were harvested 48 hrs and 72 hrs after transfection and transduced in HeLa or HEK293T cells with 8 μg/mL polybrene (Sigma). Cells were then directly used in experiments or optimized for expression by FACS.
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bioRxiv - Cancer Biology 2022Quote: ... 5 weeks and 8 weeks old C57BL/6J mice were administered with E2 (Cat No. E-2758, Sigma, MO, USA), which was dissolved in ethanol and diluted in corn oil (Cat No ...
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bioRxiv - Neuroscience 2022Quote: Tissues from Tmem150c-/- mutants and wild type mice were lysed with 8 M urea buffer and protein concentrations were determined using the Bradford reagent (Sigma). Proteins were separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... Each 15 μg sample of protein was separated using 8% SDS polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a nitrocellulose membrane (Sigma). The membrane was blocked with 5% not-fat milk in 0.1M Tris-buffered saline (pH 7.4)/Tween 0,1% (TBSTween ...
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bioRxiv - Cancer Biology 2022Quote: ... Virus-containing medium was collected 48 and 72 h after transfection and was then added to cells supplemented with 8 mg/mL polybrene (TR-1003; Sigma) for lentiviral transduction ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in lysis buffer (8 M Urea; 50 mM Tris pH 8.0; 75 mM NaCl; cOmplete protease inhibitor cocktail (Sigma 5892791001); 10 mM sodium fluoride (Sigma 919) ...
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bioRxiv - Bioengineering 2022Quote: ... 20 μl aliquots of cell-laden collagen were mixed with 10 μl of a thrombin solution (8 U/mL) and added to 10 μl of 5 mg/mL fibrinogen solution (cat #f3879, Sigma). A final concentration of 9×106 cells/mL was used in this study ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were incubated in picrosirius red (PSR) staining solution 1% (w/v) Direct Red 80 (2610-10-8; Sigma-Aldrich) in saturated picric acid (P6744-1GA ...
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bioRxiv - Genomics 2022Quote: ... desired number of cells was resuspended in differentiation medium and 8*105 cells per well were seeded in a fibronectin-coated (10 □g/mL, Millipore) 6-well plate in differentiation medium ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to the culture media for 8 hours before preparing protein lysates with the above lysis buffer supplemented with N-Ethylmaleimide (25mM, Sigma). Lysates were pre-cleared using Protein G sepharose beads (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed by separation on SDS-PAGE gels (8-12%) and transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore). Immunoblots were imaged using an anti-Ssa1/2 polyclonal antibody at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2022Quote: The tissue section on the Stereo-seq chip (5 cm x 3 cm or 2 cm x 3 cm) was then incubated at 37°C for 5-8 min and subsequently fixed in methanol (Sigma, 34860 ...
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bioRxiv - Neuroscience 2020Quote: ... was transfected into COS-7 cells cultured in a well of 8 well chamber slide glass using GeneJuice Transfection Reagent (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... 10 µg per sample using two independent microglial lysates per genotype were loaded on a bis-tris acrylamide gel (8% or 12%) and subsequently blotted onto either a PVDF or nitrocellulose membrane (Millipore) using the following antibodies ...
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bioRxiv - Microbiology 2020Quote: ... 30 million eHAP cells were seeded and transduced with gene-trap retrovirus harvested from three combined harvest in the presence of 8 μg/mL protamine sulfate (Sigma). The transduced cells were expanded and passed for a period of 10 days upon transduction ...
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bioRxiv - Microbiology 2020Quote: ... Both parasite populations were synchronised again at 0-8 hpi in the following cycle (generation 2) and 2.5 mM glucosamine (GlcN, Sigma #G4875) was added to induce PfHP1 depletion (+Shield-1/+GlcN and −Shield-1/+GlcN) ...
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bioRxiv - Microbiology 2021Quote: ... the cell culture supernatant was filtered with a 0.22 µm filter and concentrated for 90 minutes by ultracentrifugation using a 50.2 Ti rotor at 150,000 x g through a 5 mL 8% OptiPrep (Sigma-Aldrich, MO) cushion to collect virus-like particles (VLPs) ...
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bioRxiv - Immunology 2020Quote: ... the viral supernatant was harvested and used to transduce 25,000 mouse primary keratinocytes seeded in 48-well plates in the presence of 8 µg/ml polybrene (Sigma Aldrich). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... FVB/NJ primary microglia harvested from mixed glia cultures by the shaking method were seed onto Millicell cell culture inserts with 8-μm pore filter (Millipore), and treated with 50 μM GM1 for 4h or with 10 μM L-t-PDMP or 5 μM GENZ-123346 for 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved on 8-15% SDS-PAGE depending on protein size and transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked with PBS-T (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were rinsed in de-ionized water and re-fixed in freshly made Phosphomolybdic/Phosphotungstic/dH2O (1:1:2) acid solution for 8 min (#HT153, #HT152, Sigma), and then they were stained in Aniline Blue solution for 5 min (#HT154 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then plated onto LabTech II 8-well glass bottom chambers modified with 0.1% Poly-L-Lysine (PLL, Sigma P8920) and allowed to adhere for at least 4 hours at 4C before performing confocal microscopy.
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bioRxiv - Biophysics 2021Quote: ... After gelation for 2 h at RT in a humidified chamber the samples were transferred into digestion buffer (8 U/ml Proteinase K (P4850, Sigma), 50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... was flowed into the chamber (final concentrations in buffer: 8 mg/mL glucose, 0.15 mg/mL catalase (from Aspergillus niger: Millipore Sigma, formerly EMD Millipore ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Parasites were incubtaed in 96 well plates with riboflavin analogues (compounds 1-6 were obtained from the the National Cancer Institute, DCTD/DTP/DSCB, Rockville, MD, USA, and compound 7 and 8 were purchased from Sigma). All compounds were prepared in DMSO except compound 2 which was dissolved in a mixture of DMSO and 1N KOH ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Pelleted DNA was resuspended in 1 ml of TLE and washed twice by dyalisis with 8 ml of TLE using AMICON Ultra Centrifuge Filter Unit 15mL 30K (Millipore UFC903024 ...
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bioRxiv - Immunology 2022Quote: ... The T cells were resuspended at concentration of 4×106/ml in TCGF with 8 µg/ml polybrene (Sigma, 107689) and this suspension was plated at 500 µl/well in 12-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... 8-10 worms were randomly picked and aligned after placing in a drop of 10 mM tetramisole (Cat. T1512, Sigma) shortly ...
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bioRxiv - Cancer Biology 2022Quote: ... RMS cells were infected with viral particles for 24h at 37° C using 8 mg/mL of polybrene (EMD Millipore). Cells were selected for using 1 mg/ml G418 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 3X MIC and the maximum drug concentration (8 µg/mL) were stained (after reading the MIC) with 10 µg/mL of Calcofluor White (Sigma) for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... and 1-2x 104/cm2 cells were replated on a vitronectin-coated plate in Essential 8 complete medium supplemented with either 2uM thiazovivin(Sigma) or 1X RevitaCell Supplement ...
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bioRxiv - Microbiology 2019Quote: ... KSRP post-translational modifications were monitored in Huh7.5-SGR and Huh7.5 cells grown on Nunc Lab-Tek Chamber Slide™ system 8 wells (Permanox, Sigma-Aldrich), 10,000 cells/well ...
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bioRxiv - Genomics 2019Quote: ... sorted samples were re-suspended in 1ml Cell Lysis Buffer (10mM Tris pH 8, 10mM NaCl, 0.2% NP-40/Igepal) containing fresh protease inhibitors (Sigma P8340) and 1mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2019Quote: ... 37 °C for 90 secs and samples fixed by addition of an equal volume of 2x fixative (8% paraformaldehyde/0.015% glutaraldehyde, Sigma-Aldrich). Cells were permeabilised with 0.1% Triton X-100/PBS for 10 mins and blocked with 3% BSA/PBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...