Labshake search
Citations for Millipore Sigma :
3551 - 3600 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... virus-containing culture supernatant was harvested and added to the growth medium of cells in combination with 2.5-8 μg/ml polybrene (EMD Millipore). Populations of each cell line were selected by FACS or antibiotics (Blasticidin ...
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bioRxiv - Cancer Biology 2022Quote: Cells were incubated with lentiviral supernatant for 24 h in the presence of 8 μg/μl of polybrene (Sigma-Aldrich) at a multiplicity of infection >5 unless otherwise stated.
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bioRxiv - Cell Biology 2022Quote: ... with N2 (Thermo, 17502048), B27 (Thermo, 12587010), Glutamax (Thermo, 350050061) and freshly supplemented with 8 μM of CHIR (Sigma, SML1046) and 25 ng/ml of BMP4 (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of the filtered viral particles and 8 µg/ml of hexadimethrine bromide (polybrene) (Cat. Nº: Sigma-Aldrich. 107689). The following day media was change with fresh media ...
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bioRxiv - Cell Biology 2022Quote: ... to sort each well containing 80 nuclei per well into new four 96-well plates (in total of 384 wells) containing 8 μL primer dilution buffers (15 pmol primer i7, 15 pmol primer i5, 200 ng BSA (Sigma), 0.027% SDS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were separated by 8–15% sodium dodecyl sulfate-polyacrylamide gels and transferred to a PVDF membrane (IPVH00010 Millipore Immobilon). After transfer ...
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bioRxiv - Genetics 2022Quote: ... 8 to10 weeks of age female mice were administered with isoproterenol (30 mg per kg body weight per day, Sigma) for 21 days using ALZET osmotic minipumps ...
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bioRxiv - Genetics 2022Quote: ... 0.2 × 106 mESCs were seeded per 12-well and transduced the next day with 50 μl of concentrated viral supernatant and 8 ng/μl polybrene (Sigma). Antibiotic selection was started 2 days after transduction and kept until all cells in the non-transduced control were dead (typically within 1-2 passages).
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged at 12,000 RPM for 8 min at 37°C and cleared by passing through 0.22 μm Spin-X tube filters (Sigma–Aldrich).
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bioRxiv - Microbiology 2022Quote: ... Adult mosquitoes of both species were provided with a sugar solution containing 8% fructose (Emprove® Chemicals, Sigma, MO, USA) ad libitum.
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bioRxiv - Molecular Biology 2022Quote: ... cells were seeded into 12-well tissue culture trays at a density of 2×105 cells per well and pretreated with 10-8 M phorbol 12-myristate 13-acetate (PMA) (Sigma) for 72 h ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 24-well plates (1 x 105 cells x ml-1) and incubated for 24 h before 8 µg x ml-1 Polybrene (Sigma) was added to the cells ...
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bioRxiv - Plant Biology 2022Quote: ... and incubated for 8 h followed by treatment with 5 μM EPFL6 for 1 h in the presence of 2 μM MG132 (Sigma). The ubiquitinated ERECTA was detected with an α-HA (ab18181 ...
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bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... were first mixed on a magnetic stirrer for 30 min and then 8 drops of DMP-30 accelerator (#45348 Sigma) were added (Knott et al. ...
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bioRxiv - Physiology 2023Quote: ... male and female mice 8-10 weeks of age were injected (i.p.) with 40 mg/kg tamoxifen (Sigma-Aldrich, T5648) for five consecutive days ...
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bioRxiv - Physiology 2023Quote: ... The culture medium was replaced with a lentivirus viral medium supplemented with 8 μg/ml polybrene (Sigma, St. Louis, Missouri). 8 hours later ...
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bioRxiv - Molecular Biology 2022Quote: 15-30 μg of protein extract were resolved on an 8% or 12% polyacrylamide-SDS gel and transferred to a polyvinylidene difluoride membrane (PVDF, Millipore). Primary antibodies were used at the recommended dilutions in 5% milk or 3% BSA diluted in TBS-Tween ...
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bioRxiv - Plant Biology 2022Quote: ... Sections of 7 to 8 mm were then transferred to slides treated with 100 mg/mL poly-L-Lys (Sigma) and dried on a warm plate at 40 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 1 x 106 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 μg per ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in lysis buffer (50 mM Tris pH 8, 1 M NaCl, 10% v/v BugBuster (Novagen), 10 mM imidazole ...
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bioRxiv - Cancer Biology 2022Quote: LLC cells were treated with conditioned media from inflammasome-induced macrophages as indicated and 5 × 104 cells per well were seeded in top chambers of the transwell chamber (8 μM pore, 24-well plate, Millipore-Sigma) in FBS-free media with membrane inserts ...
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bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were fractionated using SE250 Mighty Small II (Hoefer Scientific Instruments) on SDS-PAGE minigels (1.5 mM thick, 8% polyacrylamide) and transferred to Immobilon-P membranes (Millipore IPVH00010) using a Bio-Rad Mini Trans-Blot (#1703930 ...
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bioRxiv - Cell Biology 2024Quote: ... Retroviral supernatant containing various C/EBP constructs was added to dKO-B cells together with 8 ug/mL polybrene (Sigma). Cells were infected by spinoculation at 2000 rpm at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... leaf pieces were transferred onto solid (0.8 % phyto agar [Duchefa]) MGI medium (full-strength MS medium containing 1.6 % glucose [Roth], 1 mg/l BA [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: ... The viral supernatant was filtered through 0.45 µm filter and added to the target cells along with 8 µg/ml polybrene (Sigma, H9268). Following one to three infections ...
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bioRxiv - Molecular Biology 2024Quote: ... protein was loaded onto a 10% or 8% SDS-PAGE gel and was transferred to Immobilon-FL PVDF membrane (IPFL00005, Millipore). The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041 ...
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bioRxiv - Developmental Biology 2024Quote: ... the Epiblast was separated from the Visceral Endoderm by 8 minutes incubation on ice in a solution of 2.5% pancreatin (Sigma, P3292) and 0.5% trypsin (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... and 25 mM Tris pH 8) and concentrated with centrifugal concentrators (10 kDa MWCO, Millipore Sigma-Aldrich, Burlington, MA, USA) to a final volume of 0.1 mL ...
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bioRxiv - Cell Biology 2024Quote: ... were resuspended at cell densities of 8×106 cells/mL for each cell type into a hydrogel comprised of 6.5mg/mL fibrin (Sigma #341573) and supplemented with 0.2mg/mL fibronectin (Sigma #F1141 ...
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bioRxiv - Cell Biology 2024Quote: ... were separated by 8 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore) at 120 V for 2 hours using transfer buffer (25 mM Tris-base ...
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bioRxiv - Genomics 2023Quote: ... cells were transduced with the GFP-pilot lentiviral supernatant supplemented with 8 μg/ml polybrene (Sigma-Aldrich TR-1003-G) at MOI ∼0.1 ...
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bioRxiv - Immunology 2024Quote: ... 1.4×108 human primary CD4+ T cells were transduced with lentivirus at a multiplicity of infection of 2 with 8 µg/ml dextran (Sigma). Virus and DynaBeads were removed after 3 days of transduction ...
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bioRxiv - Genomics 2024Quote: ... The spinfection was carried out at room temperature for 30 minutes spinning at 1000g and the cells were supplemented with 8 μg/ml polybrene (hexadimethrine bromide, Sigma). 2 µg/ml puromycin was added to the cells 24 hours post infection ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Cancer Biology 2024Quote: ... zebrafish larvae (8-9 per condition) were dissociated into single cells by applying enzymatic digestion using 5µl of liberase (Sigma-Aldrich) (2.5mg/ml ...
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bioRxiv - Biophysics 2024Quote: The purified protein was concentrated to 5–8 mg/ml using a 50 kDa molecular weight cutoff (MWCO) centrifuge concentrator (Millipore) and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2024Quote: ... Gene expression was induced at OD600 ∼0.8 by addition of either 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH) or 0.2 µM anhydrotetracycline (Sigma). Cells were grown overnight at 22°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 17 β-estradiol was added to the drinking water twice a week at a final concentration of 8 μg/mL (cat# E2758; Sigma). CHK2 activator (3 ...
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bioRxiv - Immunology 2023Quote: ... 8-10 pieces (ca. 150mg total) were placed in each cryovial containing 1mL Cryostor CS10 (Sigma Aldrich, St. Louis, MO) or 1mL 90% FBS/10% DMSO and placed in Corning CoolCell LX Cell Freezing Container (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: MIN6 cells stably expressing Cas9 were infected with this lentivirus at half volume with 8 μg/ ml polybrene (TR-1003-G, Sigma) overnight ...
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Prior to fixation we live-stained all the embryos with either Caspase-3/7 or Caspase-8 (Supplementary Table 30) for 30 min and subsequently removed the zona pellucida using Acidic Tyrode’s Solution (Sigma-Aldrich) for 30 sec – 2 min and subsequently washed in PBS/2%BSA.
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bioRxiv - Cell Biology 2023Quote: ... the reaction between peptide conjugate and crosslinkers (PEG-4VS and PEG-4 ACLT) was performed in stoichiometric ratio 1:1 in30mM HEPES buffer at pH 8 (Sigma) diluted in 1x HBSS (Gibco) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The mid log phase cells were diluted to 104 cells/ml (assuming 1 OD = 8×108 cells/ml) into LB with 0.005% Antifoam 204 (Sigma A6426) and 1 mg/ml BSA (Sigma-Aldrich A2153-100G) ...
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bioRxiv - Microbiology 2023Quote: ... supernatant containing lentivirus was filter sterilised and combined with TREx BCBL1-Rta cells in 8 μg/ml polybrene (Merck Millipore). After 8 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and plated on 35mm dishes (8×105 cortical cells and 4×105 hippocampal cells per dish) coated with poly-DL-ornithine (Sigma), in DMEM (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... the developing step took place by submerging SU-8 coated wafer into propylene glycol monomethyl ether acetate (PGMEA; Sigma-Aldrich) solution and incubating in it for 8 min with periodical agitation ...