Labshake search
Citations for Millipore Sigma :
3801 - 3850 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...
-
bioRxiv - Immunology 2023Quote: ... 20 mg of purified human IgG in PBS was immobilised to CNBr-activated Sepharose (Sigma) (10 mg IgG/ml packed Sepharose) ...
-
bioRxiv - Microbiology 2023Quote: ... Monocytes were selected by adhering PBMCs in RPMI with 5% pooled AB human serum (Sigma), 1 mM HEPES (Gibco ...
-
bioRxiv - Bioengineering 2024Quote: ... AS Diagnostik) and polyclonal rabbit anti-human FHL2 (2 µg/mL, HPA006028, Sigma/Prestige Antibodies) was performed at 4 °C overnight in PBS/10% AB serum ...
-
bioRxiv - Biochemistry 2024Quote: ... Human CXCL12 [50 ng/mL] and EGF [50 ng/mL] (Sigma Aldrich SRP3276 and SRP6253) were prepared in serum-free DMEM and used as chemoattractant in the lower chamber ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were double-immunolabeled for human nuclei and either Htt (1:200; clone EM48, Millipore) or IBA-1 (1:1,000 ...
-
bioRxiv - Bioengineering 2024Quote: ... 10 µg/L human epidermal growth factor (hEGF; E9644, Sigma-Aldrich, St. Louis, MO, USA), 1% ITS-X supplement (094-06761 ...
-
bioRxiv - Microbiology 2024Quote: ... TSB-grown bacteria were centrifuged and resuspended in human serum from male AB plasma (Sigma) and incubated for 16 h at 37 °C with shaking (180 rpm) ...
-
bioRxiv - Immunology 2024Quote: ... which were coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO; C7521). Ex Plus medium was added to both the apical and basolateral compartments and changed daily until the cells reached confluency on the inserts ...
-
bioRxiv - Immunology 2024Quote: Cytokines and chemokines from culture supernatants were analyzed using human cytokine MILLIPLEX Luminex (EMD Millipore). Briefly ...
-
bioRxiv - Neuroscience 2024Quote: ... Downregulation of PAK6 expression was performed utilizing an shRNA against human PAK6 (sh-1944, Sigma). Western blot in SH-SY5Y cell extracts was performed with anti-PAK6 (Abcam ...
-
bioRxiv - Biophysics 2021Quote: ... batch number: SLCC4853) and poly(ethylene glycol) (PEG 8000, Mv 8 kg/mol, batch number: MKBT7461V) were purchased from Sigma-Aldrich. 1 ...
-
bioRxiv - Cell Biology 2020Quote: ... to cells together with polybrene transfection reagent at a final concentration 8-10 mg/mL (Cat# TR-1003-G, EMD Millipore). 48 h post-infection ...
-
bioRxiv - Cell Biology 2020Quote: ... a polycarbonate filter (8-μm pore size) (GE Infrastructure, Fairfield, CN, USA) was coated with a solution containing 0.2% gelatin (Sigma-Aldrich Co.) and inserted between the chambers ...
-
bioRxiv - Cell Biology 2020Quote: ... streptavidin-sepharose beads were resuspended in 200 µl of 50 mM ammonium bicarbonate pH 8 containing 1 µg of trypsin (Sigma-Aldrich). Samples were then incubated at 37°C overnight with mixing on a rotating disc ...
-
bioRxiv - Cell Biology 2019Quote: ... perm-1 adult worms were dissected in 8-10 µL of 62% ESF 921 with 15 µm polystyrene beads (Sigma-Aldrich) on a 22 x 50 mm coverslip ...
-
bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
-
bioRxiv - Microbiology 2021Quote: ... 12-well plates of 293T or HUVEC cells were incubated with viral inoculum diluted in antibiotic and serum free DMEM containing 8 μg/mL hexadimethrine bromide (polybrene; Sigma-Aldrich). Plates were centrifuged at 800 x g for 2 hours at room temperature (61) ...
-
bioRxiv - Microbiology 2019Quote: ... niger cultures grown at 28°C for 7-12 days on PDA plates were harvested using 10 ml saline tween solution (8 g l-1 NaCl and 0.05 ml l-1 Tween 80) and filtered through Miracloth (Millipore; Billerica, USA) following the protocol described in [21] ...
-
bioRxiv - Developmental Biology 2021Quote: ESCs were infected with an MOI=1 for 16 hours in ESCFBS conditions supplemented with polybrene at 8 µg/mL (Sigma-Aldrich). 24h after infection ...
-
bioRxiv - Developmental Biology 2021Quote: ... the cells were washed once with Advanced DMEM and resuspended in a transduction medium (ENR media supplemented with 1mM nicotinamide, Y-27632, Chir99021, 8 μg/ml polybrene (Sigma-Aldrich)) and mixed with the previously collected concentrated virus ...
-
bioRxiv - Cell Biology 2022Quote: ... AKAP450 and AKAP450/CAMSAP2 knockout RPE1 cells were infected with lentivirus and incubated in complete medium supplemented with 8 μg/ml polybrene (Sigma-Aldrich). After 24 hrs ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein lysates were electrophoresed on 8% (LRRK2) or 15% (α-synuclein) SDS-PAGE gels and transferred overnight onto PVDF membranes (Millipore). Blots were either blocked with Everyblot blocking reagent (BioRad ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 ml deionized water, 8 mg of sucrose (A2188.1000, Axon Lab, Switzerland) and 1 mg of Amaranth dye (A1016, Sigma-Aldrich, USA), (ii ...
-
bioRxiv - Molecular Biology 2021Quote: ... the cells were washed once with Advanced DMEM and resuspended in transduction medium (ENR media supplemented with 1mM nicotinamide, Y-27632, Chir99021, 8 μg/ml polybrene (Sigma-Aldrich) and mixed with concentrated virus ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 °C and suspended in resuspension buffer (50 mM Tris-HCl, pH 8, 300 mM NaCl, 2 mg/ml lysozyme (Sigma, L6876), 1 mM EDTA ...
-
bioRxiv - Cell Biology 2022Quote: ... The pellet was thawed on ice and resuspended in 50 μl SLB/c buffer (10 mM Tris-HCL pH 8, 10 mM EDTA, 1% SDS supplemented with protease inhibitor (11836170001, Sigma-Aldrich)) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
-
bioRxiv - Molecular Biology 2019Quote: ... in second attempt we used the library consisted of 8 random nucleotides with fixed G in the middle position: 5’-GCTCAAGCTTACTGCAGATATNNNNGNNNNTTTAGGATCCATCTAGAGTCCGA- 3’(SIGMA-ALDRICH). 15µg of the library were made double-stranded with 5µg 3’ primer (CAST1_R- ACTCTAGATGGATCCTACG or CAST2_R-TCGGACTCTAGATGGATCCTA ...
-
bioRxiv - Bioengineering 2019Quote: ... 200 μL of folded reaction was purified from excess staples and buffer exchanged into 20 mM Tris-HCl pH 8.0 and 8 mM MgCl2 using a 100kDa MWCO spin concentrator (Amicon, Merck Millipore, Billerica, MA). The concentration was subsequently adjusted to 5 nM ...
-
bioRxiv - Microbiology 2019Quote: ... resuspended in 40 ml of Buffer A (50 mM Tris-HCl pH 8, 500 mM NaCl) in presence of cOmplete™ EDTA-free protease inhibitor (Sigma) and lysed using an EmulsiFlex-C3 homogenizer (Avestin) ...
-
bioRxiv - Cancer Biology 2019Quote: ... The membrane was UV-crosslinked for 3 minutes/each side and hybridized with probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) and developed as described62 ...
-
bioRxiv - Cancer Biology 2019Quote: ... the gel was blotted and probed with antisense telomere probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) or with a 1.6 kb fragment containing the sequence (TTAGGG)n62 ...
-
bioRxiv - Biochemistry 2019Quote: ... 43-48 h after induction, cells were harvested and resuspended in buffer A (50 mM Tris pH 8, 150 mM NaCl, 1% digitonin (Sigma-Aldrich), 6.4 mM β-mercaptoethanol ...
-
bioRxiv - Cell Biology 2019Quote: ... L-lysine-13C6, 15N2 (Lys-8, #608041)) and L-arginine-13C6, 15N4 (Arg-10, #608033) stable isotope labelled amino acids (Sigma-Aldrich). Media was also supplemented with 10% dialysed FCS (Sigma) ...
-
bioRxiv - Biochemistry 2019Quote: ... and lysed in solubilization buffer (50 mM Tris–HCl pH 8, 1% Igepal and 1 mM EDTA) with protease inhibitor cocktail (Sigma Aldrich). The cell lysate was centrifuged at 14,000×g for 20 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... Supernatants containing lentiviral particles were used to infect MDA-LM2 cancer cells overnight in the presence of 8 μg/ml polybrene (Sigma-Aldrich). Infected cells were selected with 2 μg/ml puromycin (Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... U937 cells were transduced by spin infection in which cells were suspended in viral media with 8 μg/mL polybrene (Sigma Aldrich), centrifuged at 1000 x g for 1 hour ...
-
bioRxiv - Plant Biology 2020Quote: ... The fixative was washed away with phosphate buffered saline buffer supplemented with Tween 20 (PBST) and cells were treated for 8 min at room temperature with a solution of 2% (w/v) Driselase (Sigma-Aldrich) in 0.4 M mannitol ...
-
bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 8–20 mg/mL (Amicon Ultra 10 kDa cut off, Millipore Sigma). Protein samples were stored at 4°C until use.
-
bioRxiv - Bioengineering 2021Quote: PBMC-iPSCs were transduced overnight with lentiviruses that co-express dCas9-VPR and the puromycin resistant gene (EF1a-dCas9-VPR-P2A-Puro) in the presence of 8 µg/mL of polybrene (Sigma-Aldrich). Two days post-transduction ...
-
bioRxiv - Bioengineering 2020Quote: ... The cell culture medium was replaced with 2 mL fresh medium containing 8 µg/mL hexadimethrine bromide (Sigma Aldrich, H9268-5G), 20 µL ViralPlus Transduction Enhancer (Applied Biological Materials Inc. ...
-
bioRxiv - Cell Biology 2021Quote: Cells treated with SPS either alone or in conjunction with bafilomycin-A1 were collected and subsequently lysed in lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP-40) supplemented with PhosStop phosphatase inhibitor cocktail (Sigma Aldrich) and protease inhibitor cocktail (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
-
bioRxiv - Physiology 2021Quote: ... All animals were males that were intraperitoneally injected at 8 weeks of age for 5 consecutive days with tamoxifen (Sigma, T5648) freshly dissolved in corn oil (Sigma ...
-
bioRxiv - Biochemistry 2021Quote: ... protein amounts ranging from 50 and 100 μg of protein were diluted with H2O to perform 200 μL and an additional 200 μL of 8 M urea (Sigma-Aldrich), 75 mM Tris-HCl (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2021Quote: ... adherent cells were incubated with 1 ml of lentiviral medium diluted in 1 mL of serum-supplemented medium containing 8 μg/μL of polybrene (Sigma/Aldrich). After 4 h ...
-
bioRxiv - Cell Biology 2021Quote: ... transduction of cells was performed with a multiplicity of infection equal to 3 in the presence of 8 µg/ml Polybrene (H-9268; Sigma-Aldrich). Cells were then selected with 2.5 µg/ml puromycin for 2 days ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
-
bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...