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Citations for Millipore Sigma :
3601 - 3650 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Viruses were titered and then used to transduce cells supplemented with 8 µg/ml protamine sulfate (Sigma-Aldrich, USA #P3369).
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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bioRxiv - Developmental Biology 2023Quote: ... pharyngeal tissues were obtained by treating the wall of the embryonic pharynx with a solution of pancreatin (8 mg/ml, Sigma) for 30–90 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... DRG explants from E12.5 mouse embryos were dissected as described above and cultured for 18-24 hours in DRG growth medium on 8-chamber glass slides coated with 1 mg/ml poly-D-Lysine (PDL) (Sigma) and 5 μg/ml laminin (Millipore).
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Cancer Biology 2023Quote: Non-tumor tissue was transferred into a 50 ml tube pre-filled with 25 ml dissociation/chelation buffer (8 mM EDTA, 0.5 mM DTT, Dnase I (100 units/ml, Millipore Sigma)) ...
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bioRxiv - Neuroscience 2023Quote: 10 female frozen heads per tube were homogenised in 2% SDS buffer (Cat No. 42- 801-8 0ML, Millipore Sigma) containing 1X RIPA buffer (Cat No ...
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bioRxiv - Molecular Biology 2023Quote: ... were subjected to SDS-PAGE with 8% or 15% Tris-Glycine gels and transferred to Immobilon- P PVDF membranes (Millipore) using a TransBlot SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate supernatant was filtered with a 0.33 µm filter unit and loaded onto a 5 mL Ni-NTA column (17528601 Cytiva) pre-equilibrated with buffer A (20 mM Na-HEPES pH 8, 300 mM NaCl, 20 mM Imidazole (I202 Sigma) pH8) ...
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bioRxiv - Immunology 2023Quote: ... USA) cells and the respective sublines were maintained in RPMI-1640 medium containing 8% fetal calf serum (FCS) (Sigma, F7524), 2 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted with 6x Laemmli buffer (70% v/v Tris HCl pH 6.8, 10% w/v SDS, 30% (v/v) glycerol (Carl Roth, 3783) and 0.01% w/v Bromophenol blue (Sigma, B0126) containing 10% v/v b-mercaptoethanol (Carl Roth ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed twice with lysis buffer and proteins were eluted from the beads with 8 M urea (U5128; Sigma) and 0.1 M Tris–HCl at pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor cell lines and UPCs were infected with the concentrated virus in a culture media containing 8 µg/ml Polybrene (Sigma) and positively transduced cells selected by FACS for the expression of the corresponding fluorescent reporter.
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bioRxiv - Bioengineering 2022Quote: ... hiPSCs were collected and centrifuged at 300 g for 5 minutes and resuspended in Essential 8 Flex medium containing 2μM ROCK inhibitor (Thiazovivin) (Millipore Sigma). hiPSCs were counted using a Moxi cell counter (Orflo Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Where indicated an RGD peptide (sequence - Acetyl-Cys-Doa-Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2; Doa = 8-amino-3,6-dioxaoctanoic acid, Millipore Sigma) was added to the cell suspension at a concentration of 0.5 mM ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... The target cells were infected overnight by filtered viral supernatant in the presence of 8 μg/mL of polybrene (#TR-1003-G, Millipore). The infected cells were selected with puromycin (2 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Boyden chamber invasion tests were conducted using Millicell with hanging Transwell polyethylene terephthalate inserts (8 µm pore size, Millipore, PIEP12R48). 200 µl of serum-free medium containing 5104 unstimulated MG63 or LM7 cells was added to the upper compartment ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen cell pellets were thawed and then lysed using ice cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 (Sigma), 10 mM NaCl (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown in complete medium and for days 8-14 this medium was supplemented with 50μM L-ascorbic acid (Sigma-Aldrich). Medium changes were performed every 3 days ...
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bioRxiv - Biophysics 2023Quote: ... Supernatant post-48/72h transfection was collected for transduction in target cells supplemented with 8 µg/mL polybrene (EMD Millipore). Single cell clones of Cas9 and sgKRAS-transduced cells were sorted into 96-well plates using a FACSMelody benchtop sorter (BD Biosciences ...
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bioRxiv - Genomics 2023Quote: ... and 8 µg of the plasmid of interest were mixed with 200 µl of CaCl2 (1 M, Sigma, 21115-100ML). Volume was completed with sterile water up to 800 µl ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed by spinoculation at 1200 X g for 2 hours at 32° C in the presence of virus particles and 8 ug/ml polybrene (Millipore). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... 600,000 cells were seeded into each well of a 6-well plate and one week later were infected with up to 100ul virus per well (and 8 ug/ml polybrene (TR-1003-G, Sigma)) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 800µl NP-40 buffer (20mM Tris pH=8, 150-200mM NaCl, 0.5mM EDTA, 1% NP-40, 1mM DTT, 5% glycerol and protease inhibitor cocktail; Sigma-Aldrich). Protease inhibitor cocktail (Bimake ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 300μl extraction buffer (50mM Tris pH=8, 150mM NaCl, 0.5mM EDTA, 1% NP-40, 5%, 1mM DTT, and protease inhibitor cocktail [Sigma-Aldrich]). Cell extracts were sonicated and centrifuged at 4°C for 15 min at 16,000 ×g ...
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bioRxiv - Cell Biology 2023Quote: ... filtered and applied onto the desired HeLa cells for 24-48 h in the presence 8 mg/ml polybrene (Sigma). Cells were then recovered and expanded in growing DMEM for 5-7 days prior to sorting by FACS to match protein expression levels where possible.
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bioRxiv - Immunology 2023Quote: ... Lentiviral supernatants were used to infect Ramos cells via spinfection (2350 rpm for 90 min) in the presence of 8 μg/ml Polybrene (Sigma). Flow cytometry (BD Aria III sorter ...
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bioRxiv - Microbiology 2022Quote: ... nuclei were resuspended in one volume of RLN buffer (50 mM Tris-HCl pH 8, 140 mM NaCl, 1.5 mM Mg2Cl (Sigma-Aldrich), 10 mM EDTA pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates containing 50 µg protein were separated by electrophoresis on 8–12% polyacrylamide gels and the separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore, USA) at 200 mA for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... JCPyV-infected and mock-infected astrocytes were detached using TrypLE (GibcoTM) and replated at 30 000 cells/cm2 on 8-well Milicell EZ glass chamber slides (Millipore). Following a 24-hour incubation period ...
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bioRxiv - Neuroscience 2023Quote: ... JCPyV-infected and mock-infected astrocytes were detached using TrypLE (GibcoTM) and replated at 30 000 cells/cm2 on 8-well Milicell EZ glass chamber slides (Millipore). The next day ...
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bioRxiv - Plant Biology 2023Quote: ... 8-day-old plants (n=15) were dipped for 8 minutes in Lugol staining solution (0.1% I, 1%KI, Sigma-Aldrich) and observed in an Eclipse E200 Microscope (Nikon ...
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bioRxiv - Cell Biology 2023Quote: ... Virus particles were collected 48 h after transfection then filtered through a 0.45 μm syringe filter and used to infect MCF-10A cells in the presence of 8 μg/ml polybrene (Sigma). Clones of interest were selected using 1 μg/ml puromycin (Sigma).
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon tissues from one wild type and one Olfr78-GFP KO mice (respectively 8 and 6 months-old) were cut in small pieces and digested with 0.5 mg/ml Collagenase I (Sigma Aldrich) for 25 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Immunology 2023Quote: ... The tissue was cut into very small pieces using a clean razor blade and transferred into 0.3 mL RIPA buffer containing 50 mM Tris-HCl pH7-8 (Sigma-Aldrich), 150 mM NaCl (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: P5 Aldh1l1:eGFP mice were anesthetized with ketamine-xylazine (140 and 8 mg/kg, respectively, i.p.) and injected intraperitoneally with 50 µL of puromycin (P7255, Sigma Aldrich) diluted in water (10 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: Gastrocnemius muscle samples were lysed in lysis buffer (10% N-Deoxycholate, 0.1% SDS, 1% Triton X-100, 10 mM Tris pH 8, 140 mM NaCl, phosphatases/proteases inhibitors [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2024Quote: ... leaf pieces were transferred onto solid (0.8 % phyto agar [Duchefa]) MGI medium (full-strength MS medium containing 1.6 % glucose [Roth], 1 mg/l BA [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2024Quote: ... Retroviral supernatant containing various C/EBP constructs was added to dKO-B cells together with 8 ug/mL polybrene (Sigma). Cells were infected by spinoculation at 2000 rpm at 37°C for 1 hour ...
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bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA or RNase H digested DNA were immunoprecipitated with 8 μg of S9.6 antibody (Millipore #MABE1095) overnight at 4°C in DRIP binding buffer (10 mM sodium phosphate ...
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bioRxiv - Microbiology 2023Quote: ... Total cell lysate samples were then separated on 8-12% SDS-PAGE gels and transferred to PVDF membranes (Millipore, ISEQ00010). Membranes were blocked with TBST containing 5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Harvested cells were resuspended in lysis buffer (50 mM HEPES, pH 8, 200 mM NaCl, 0.5mM TCEP, 5 U/mL benzonase (Millipore Sigma), EDTA-free cOmplete protease inhibitor cocktail tablet (Roche) ...
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bioRxiv - Genomics 2023Quote: ... per 0.1 g of ground tissue powder was resuspended in 400 µL of lysis buffer (100 mM Tris-HCl [pH 8], 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393] ...
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bioRxiv - Genomics 2023Quote: ... per 0.1 g of ground tissue powder was resuspended in 400 µL of lysis buffer (100 mM Tris-HCl [pH 8], 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393] ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrated virus equivalent to that harvested from half a 10 cm dish of HEK293T cells was added to 2 × 105 BEL-A cells in the presence of 8 μg/mL polybrene (Sigma) for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... and lysed using a solution of 4% SDS and 50 mM TRIS-HCl (pH=8) containing protease inhibitors (Sigma-Aldrich) as described previously79 ...