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Citations for Millipore Sigma :
3501 - 3550 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The PCR fragments with appropriate restriction sites was cloned into the BamHI and SalI sites of pET28a (Novagen) and pGST4CS ...
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bioRxiv - Neuroscience 2022Quote: Digoxygenin (DIG)-labeled probes were produced with the SuperScriptTM III One-Step RT-PCR kit (Sigma 12574-026) using specific primers for the Bgl-FaNaC transcript (Forward ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore) and were found to be negative for mycoplasma.
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore) and were found to be negative for mycoplasma.
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bioRxiv - Microbiology 2024Quote: ... Each cell line was tested monthly for mycoplasma using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich, # MP0035) and confirmed mycoplasma free ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse genotypes were determined by PCR using genomic DNA extracted using the REDExtract-N-Amp kit (Sigma XNAT) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... mice DNA was extracted from ear notches or tails using the RED ExtractN-Amp Tissue PCR Kit (SIGMA). For Lrp1flox ...
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bioRxiv - Microbiology 2024Quote: ... Cells were inspected for mycoplasma contamination using a VenorTM GeM Mycoplasma Detection kit PCR-based (Sigma, MP0025-1KT).
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bioRxiv - Plant Biology 2022Quote: The full-length coding sequence of GGP1 was amplified by PCR and cloned into the pET28a vector (Novagen). The GGP1 enzyme was expressed in E ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were confirmed to be negative for mycoplasma using LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich, MP0035).
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was performed in triplicates with the KAPA SYBR® Fast qPCR Master mix (Sigma-Aldrich, KK4600) using a Rotor-Gene Q (Qiagen ...
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bioRxiv - Microbiology 2023Quote: Viral barcodes were amplified in 50 µl PCR reactions using KOD Hot-Start Master Mix (Sigma-Aldrich, 71842). For the viral supernatant samples ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... using the pPB_EF1_MCS-EGFP_IRES_Puro as a PCR template and utilising primers with the respective knockin-overhangs (ordered from Sigma-Aldrich). Gel-extracted PCR products (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragment of each miRNA was cloned into a pT7Blue T-Vector (Novagen, Inc, Madison, WI, USA). The recombinant plasmids were introduced into competent cells of Escherichia coli JM 109 ...
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bioRxiv - Cancer Biology 2023Quote: ... Mycoplasma testing was routinely performed using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MA, USA, MP0035-1KT) to ensure the absence of contamination before cells were used in experiments.
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bioRxiv - Biochemistry 2023Quote: ... Jurkat were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Indexing PCR reactions were pooled prior to purification with Sera-Mag SpeedBeads™ carboxylate-modified hydrophilic (Sigma-Aldrich). An initial bead concentration of 0.5X was used to remove long fragments that were likely contaminants of fresh DNA ...
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bioRxiv - Microbiology 2023Quote: ... Figure modified from D’Andreano & Francino27.Presence of PCR products was assessed using 0.5% agarose (Sigma-Aldrich, USA, A9539) gel electrophoresis with SYBR Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Cells were screened negative for mycoplasma contamination by monthly scheduled testing (LookOut® Mycoplasma PCR Detection Kit, Sigma). Stable cell lines expressing Sm.TRPMPZQ or Fh.TRPMPZQ were generated using the Flp-In T-REX core kit (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Offspring were screened for Taf7l mutations using REDExtract-N-AmpTM Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA) extracted genomic DNA from tail-tip biopsies ...
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bioRxiv - Microbiology 2024Quote: ... We confirmed the absence of mycoplasma contamination in cells using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma).
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bioRxiv - Biochemistry 2024Quote: ... Cells were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in LB broth to an OD600 of 0.1 and 5 μl used to inoculate 96-well culture plates containing 150 μl LB agar supplemented with CPRG (Chlorophenol red-β-D-galactopyranoside – Sigma) (20 μg/ml) ...
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bioRxiv - Plant Biology 2020Quote: ... First generation transformants (T1) were selected upon germination on MS medium plates containing the herbicide glufosinate-ammonium (PESTANAL®, Sigma-Aldrich). Transgene expression was assessed by real time qPCR using the primers ANAC017_F and ANAC017_R listed in Table S1 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µl of sample was spotted 1 cm from the bottom of a 20 cm x 20 cm silica gel on TLC Aluminium foil plate (Supelco Sigma-Aldrich) and the TLC plate was placed into the humidified glass chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... hCEcto stem cells were grown on collagen-coated six well-plate to 50% confluence before they were treated with virus solution and Polybrene (1μg/mL) (Sigma-Aldrich, # H9268) overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... ARPE-19 cells were seeded at semi-confluence in 12-wells plates and next day medium was replaced for growth medium containing 2mM thymidine (Sigma-Aldrich). After 24 hrs ...
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bioRxiv - Immunology 2020Quote: ... were cultured at a concentration of 1 million cells per ml in complete media on pre-incubated anti-CD3/anti-CD28 bound plates in IL-2 (20ng/ml) (Sigma-Aldrich) for 2 days and then just IL-2 (20ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... SMF cells and EMT6 cells were transduced at 50% confluency in 6-well plates with 1 mL of concentrated lentivirus and 6 µg/mL polybrene (Sigma 107689) at 1000xg and 33°C for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... The cultures in the plates were then transferred by the liquid handler into glass-bottom 384-well microscope plates (Matrical Bioscience) coated with concanavalin A (Sigma-Aldrich). After 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Then 30 μl of each sample was transferred to a 96-well plate and incubated with 100 μl of Free Glycerol Reagent (Sigma-Aldrich) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Egg chambers of interest were then transferred into a mutli-well plate with 500 μl fresh media with the addition of MG132 (EMD Millipore) or DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing 20 nmol phosphate were subjected to TLC on an HPTLC plate (Silica gel 60; Merck Millipore, MA, USA) in the solvent system hexane/diethyl ether/acetic acid (80:20:1 ...
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bioRxiv - Cell Biology 2020Quote: ... 24 well plate) to the adherent osteoblast precursors in presence of DM-medium supplemented with 1 µM PGE2 (Sigma-Aldrich, #P6532) and 10 nM 1,25(OH)2D3 (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: MutuDCs were seeded at 150,000 cells/well in U-bottom 96-well plates and incubated with 10 mg/ml β-lactamase (Sigma, #P0389) for 3 h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 shNT and shDHX30 cells were cultured in 96-well tissue culture plate and treated with 100mM 5-Fluorouracil (Sigma Aldrich) as positive control ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were cultured in 96-well tissue culture plates and after 24 hours were treated with Actinomycin D 100nM (Sigma Aldrich) as positive control ...
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bioRxiv - Molecular Biology 2020Quote: Cells were cultured on a coverslip place onto a 48-well plate and after 48 hours they were washed with PBS and fixed with 4% Paraformaldehyde (PFA) (Sigma Aldrich) for 30 minutes at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... The total number of GCR events was determined by plating the remaining culture on SC-Arg plates that were supplemented with 50 mg/L L-canavanine (Sigma C9758) and 1 g/L 5’-fluoroorotic acid (US Biological Life Sciences F5050 ...
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bioRxiv - Developmental Biology 2020Quote: For virus titration in a relevant cell type, neural NE4C cells (ATCC, CRL2925™) were cultured on a 6-well plate in MEM (M5650, Sigma) supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Developmental Biology 2021Quote: ... sorted Vav-mTmG LSK cells were seeded in 48 well plates (9000 cells/well) in Stemline II haematopoietic stem cell expansion medium (Sigma, S0192) containing 50 ng/ml SCF and 50 ng/ml flt3-ligand ...
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bioRxiv - Developmental Biology 2021Quote: ... sorted LSK cells were seeded in 48 well plates (6000 cells/well) in Stemline II haematopoietic stem cell expansion medium (Sigma, S0192) containing 50 ng/ml SCF (Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: Ovarian cancer cells were cultured for 24hrs on standard culture plates and 72hrs on Poly-2-HydroxyEthlylMethacrylate-coated culture dishes (poly-HEMA, Sigma #3932). Poly-HEMA prevents the cells from attaching to the culture dish ...
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bioRxiv - Biophysics 2022Quote: ... 100 μL of the media were transferred to a 96-wells plate and mixed with the equal volume of Griess reagent (Sigma, USA) (0.04 g/mL in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 6×105 cells were infected in 6-well plates with 125uL of high titer virus in standard media with 5ug/mL Polybrene (Sigma-Aldrich). Media was changed after 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was made with 293T cells plated at 60% confluency in 10cm tissue culture treated plates and transfected using X-tremeGENE HP DNA Transfection Reagent (Sigma # 6365779001) with lentiviral vectors ...
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bioRxiv - Genomics 2022Quote: ... GFP positive cells were plated at an average density of 0.5 cells per well in a 96-well plate (previously treated with poly-L-Lysine (Sigma #P4707-50ml) to improve cell adhesion ...
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bioRxiv - Developmental Biology 2022Quote: Wild-type E14 mouse ES cells (Sarah Kinkley Lab) were cultured without feeders on gelatin-coated plates (0.1% gelatin, Sigma-Aldrich G1393). The media was changed every day and cells were passaged every 2 days ...
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bioRxiv - Cell Biology 2022Quote: ... and the resulting F2 plants were selected on MS plates containing 50 mg/L of kanamycin (Sigma-Aldrich, catalogue number K1377). Because the G2/M-marker CYCB1;1 is strongly expressed during DNA damage46 ...