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Citations for Millipore Sigma :
3751 - 3800 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... hDPCs were stimulated with 100 ng/ml LPS (Escherichia coli O111:B4; Sigma-Aldrich, St. Louis, MO, USA). To inhibit NF-κB signaling ...
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bioRxiv - Developmental Biology 2024Quote: ... 2) 100 ng/ml Lipopolysaccharide (LPS from Escherichia coli O55:B5, L2880, Sigma-Aldrich, St. Louis, MO, US), 3 ...
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bioRxiv - Cell Biology 2020Quote: ... organoids were treated with 50μM FITC-conjugated deoxycholic acid (E. Mash, University of Arizona) or 40kDa FITC-conjugated dextran (Sigma, FD40S) (Jean-Louis et al ...
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bioRxiv - Neuroscience 2019Quote: ... uterine horns of E 13.5 pregnant dams were exposed by midline laparotomy after anesthetization with Avertin (312 mg/kg, Sigma-Aldrich). 1 μl of DNA plasmid(s ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Cell Biology 2019Quote: ... SBP-EGFP-E-cadherin was released from the ER by adding a final concentration of 40 µM D-Biotin (Sigma) in L-15 medium ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and e-cig cartridge aerosols were determined using 2’7’dichlorofluorescien diacetate (H2-DCF-DA) fluorogenic probe (Sigma-Aldrich Cat# 287810). H2-DCF-DA [5 mM] with NaOH [0.01N] was reacted for 30 minutes to prepare the dye ...
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bioRxiv - Developmental Biology 2019Quote: ... adjusted to 340±5 mOsm with H20) then treated with 20 mg/ml Pronase E in L15/FBS buffer (10% FBS and 1% Penicillin-Streptomycin -Sigma P4458-in L-15 insect medium ...
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bioRxiv - Biochemistry 2020Quote: ... ii) storage buffer (10 mM NaCl, 10 mM Tris-HCl) with 0.1 mg Pronase E from Streptomyces grisens (Sigma Aldrich) with 0.5% (v/v) ...
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently treated with 90nM cyst(e)inase in the presence of the following rescuing agents: 100uM Trolox (Sigma, 238813), 50nM ZVAD-FMK (SelleckChem ...
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bioRxiv - Cell Biology 2020Quote: ... 3d, e, Fig. 4b) or 15kPa gels (Fig. 4g, h) were treated with 15µM of Blebbistatin or DMSO (Sigma-Aldrich) in ENR medium for 3h ...
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bioRxiv - Cell Biology 2021Quote: ... the imaging media with alpha factor was removed and new media with 0.1 mg/ml Pronase E (Sigma P-6911) was added to inactivate any remaining alpha factor ...
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bioRxiv - Biochemistry 2022Quote: Purified full-length E-cadherin was concentrated to 8-10 μM and mixed with the nanodisc scaffolding protein MSP1D1 (Sigma) at a 5 fold molar excess ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 weeks and 8 weeks old C57BL/6J mice were administered with E2 (Cat No. E-2758, Sigma, MO, USA), which was dissolved in ethanol and diluted in corn oil (Cat No ...
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bioRxiv - Biochemistry 2022Quote: ... 0.25 mg/ml Pepstatin A and 0.25 mg/ml E-64) or Roche cOmpleteTM EDTA-free protease inhibitor cocktail tablet (Millipore-Sigma). NP-40 (final concentration of 0.5% ...
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bioRxiv - Neuroscience 2021Quote: ... or glass coverslips in 24-well plates (for immunocytochemistry) coated with Poly-D-Lysine (5 μg/ml, PDL, A-003-E; Millipore) and laminin (1 mg/ml Sigma L2020) ...
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bioRxiv - Genetics 2019Quote: ... Cells were released from G1 by washing with YPD containing auxin (if indicated) and 0.15mg/mL pronase E (Sigma Aldrich) three times ...
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bioRxiv - Microbiology 2021Quote: ... was incubated in 50 mM Tris-acetate buffer (pH 7.4) containing 5 mg/mL protease mixture (Pronase E, Sigma-Aldrich) in a total volume of 1 mL at 28 °C for 2 h and then directly resuspended in the standard SDS sample buffer before SDS-PAGE and western blot analysis.
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bioRxiv - Biochemistry 2021Quote: ... 0.3 ml of media were precipitated with 25 µl EZ view Red anti-HA affinity gel (E 6779; Sigma-Aldrich) according to the manufacturers’ protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Seeds were surface-sterilized and sown in Petri dishes on half-strength Murashige and Skoog (MS/2) solid medium containing 0.8 % (w/v) agar-agar type E (Sigma Aldrich) and 1% sucrose ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae in Colombia blood agar (E&O Laboratories, UK) followed by Todd-Hewitt broth plus 0·5% yeast extract (Sigma), both at 37°C in 5% CO2 ...
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Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-SeqbioRxiv - Developmental Biology 2021Quote: ... animal cap - (E) vegetal cap. Total RNA (X. laevis and A. mexicanum) was extracted using TriReagent extraction and LiCl precipitation (Sigma) (details in (Sindelka et al ...
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bioRxiv - Cell Biology 2022Quote: ... 80-mer oligonucleotides biotinylated either on the 5’ or 3’ end containing the E-box of the Lgr5 promoter or a mutated E-box (see table) were annealed with the corresponding antisense sequence and bound to streptavidin agarose beads (S1638, Millipore). Beads were washed 3 times ...
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bioRxiv - Neuroscience 2022Quote: ... and placed in a T75 flask (pre-coated with 50 μg/mL poly-D-lysine (Merck Millipore, A-003-E) for 1 hour) ...
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bioRxiv - Neuroscience 2022Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... 80 μM aprotinin, 4 mM bestatin, 1.4 mM E-64, 2 mM leupeptin and 1.5 mM pepstatin A; Sigma-Aldrich, P8340) and 50 nM calyculin A (LC Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... The pesticides were: 3,6-dichloropyridine-2-carboxylic acid (i e. clopyralid, pesticide analytical standard, CAS 1702-17-6, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Neuroscience 2024Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Genetics 2024Quote: ... in TC-treated 12- or 6well plates coated with 0.05 mg/mL Poly-D-lysine (Sigma-Aldrich A-003-E). At 3-4 DIV ...
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bioRxiv - Immunology 2023Quote: ... HEK293FT (provided by T. Brdicka, IMG) and MC-38 cells (provided by E. Palmer, University Hospital Basel) were cultivated in DMEM media (Sigma). The media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: DO11.10 cell line (provided by Tomas Brdicka, IMG) and T2–Kb (provided by E. Palmer, University Hospital Basel) were cultivated in RPMI media (Sigma). HEK293FT (provided by T ...
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bioRxiv - Neuroscience 2023Quote: ... and the gamma secretases inhibitor was purchased as Compound E (γ-Secretase Inhibitor XXI) from EMD Millipore (Burlington, MA, USA). Ammonium acetate (Cat# A11450 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were stained with hematoxylin and eosin (H&E) and for Tph1 with an anti-Tph1 primary antibody (Sigma; #T0678). Detection was performed using the polymer-based Envision FLEX+ detection system (Agilent).
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bioRxiv - Bioengineering 2023Quote: ... and 5 μm sections were taken before being baked for 3 hours at 60°C and stained with hematoxylin and eosin (H&E, Sigma) or Masson’s trichrome (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... and tumor target (T) cells were co-cultured in triplicates at the indicated E/T ratio using flat bottom poly-L-ornithine (Sigma) coated 96-well plates with 1 × 104 target cells in a total volume of 200 µL per well in NALM-6 medium ...
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bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer-exchanged against HBS-E (10 mM HEPES at pH 7, 150 mM NaCl, 2 mM EDTA) by ultrafiltration using Amicon Ultra devices (Millipore).
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bioRxiv - Cell Biology 2023Quote: Transient knockdown of S100A11 and E-cadherin in MCF-10A cells was performed by transfection of MISSION® Predesigned (Sigma) or custom-made siRNAs ...
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bioRxiv - Bioengineering 2023Quote: ... samples were mounted on paraffin blocks and sectioned into 5 µm sections then stained with hematoxylin and eosin (H&E, Sigma) or picrosirius red (PSR ...
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bioRxiv - Neuroscience 2023Quote: ... brain homogenate from a RML inoculated C57BL6/J mouse at the terminal phase of disease was treated with 100 μg/ml of pronase E (Sigma) for 30 min at 37 °C with shaking in a thermal mixer at 800 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... The first injection of pre-treatments was a solution of ethosuximide (150 mg/kg of 84 mg/ml in phosphate buffered saline, s.c.; Cat# E;7138, Sigma-Aldrich). Ethosuximide was used because the background strain ...
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bioRxiv - Developmental Biology 2021Quote: ... One group was injected intraperitoneally (i.p.) with LPS (Escherichia coli serotype 0111:B4; Sigma-Aldrich Canada, Oakville, ON, Canada) on GD13.5 (10 µg/kg ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2020Quote: ... β- and γ-Syn were produced by co-expressing PT7-7 plasmids with yeast N-acetyltransferase complex B (NatB)74 in Escherichia coli BL21 Star (DE3) cells using M9 minimal media supplemented with 0.5 g/L of 15NH4Cl (Sigma). Protein purification under non-denaturing conditions was performed as described previously75 ...
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bioRxiv - Immunology 2021Quote: ... Phorbol 12-myristate 13-acetate (P8139) and Lipopolysaccharides from Escherichia coli 055:B5 (L2880) were purchased from Sigma-Aldrich. Pam3CSK4 (tlrl-pms) ...
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bioRxiv - Biophysics 2020Quote: ... coli DH10BacY competent cells and plated on LB-Agar plates supplemented with 30 µg ml-1 kanamycin (#K4000, Sigma), 7 µg ml-1 gentamycin (#G1372 ...
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bioRxiv - Biochemistry 2020Quote: ... The WT and mutant plasmids were then transformed into Escherichia coli Rosetta™ 2(DE3)pLysS competent cells (Novagen) for protein expression.
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bioRxiv - Biochemistry 2020Quote: ... or catalytically-inactive BT4656S1_11 (in which Ser 77 is intact but prevents formation of formylglycine during expression in E.coli) were expressed in Escherichia coli strain TUNER (Novagen), and cultured to mid-exponential phase in LB supplemented with 50 μg/mL kanamycin at 37 °C and 180 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of LPS from Escherichia coli O111:B4 (sigma) in 0.5% solution of carboxymethyl cellulose (sodium salt; Sigma) was injected into the air pouch ...
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bioRxiv - Plant Biology 2021Quote: ... coli expressing FLAG-tagged RIN4 protein was incubated with 20 µL of anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2022Quote: The pEGFP-NONO_WT or pEGFP-NONO_ΔRRM1 plasmid was transformed into competent Rosetta 2(DE3)pLysS Escherichia coli cells (Novagen, 71400) and plated on LB agar plates with selection for kanamycin and chloramphenicol ...