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Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Cells were then collected and re-plated at 500 cells/well with either 5 μM 4-nitroquinolone N-oxide (4NQO) (Sigma, N8141) or a dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was centrifuged at 5,000 g for 5 minutes at 4 °C and the supernatant was added to ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich), equilibrated with the wash buffer (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... and 10x DMEM (First Link Ltd., UK) were mixed at a 4:1 volume ratio and neutralized with 5 M NaOH (Sigma, Canada). Fast Green (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... and an ATP-regeneration system (4 mM ATP, 50 μg/mL creatine kinase [Sigma-Aldrich], 5 mM creatine phosphate [Sigma-Aldrich]). The endpoint fluorescence for complete FITC-casein degradation was determined by addition of porcine elastase (100 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal) (no. B4252; Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... The nuclei were stained with DAPI (1mg/ml for 5 minutes) and the coverslips were mounted on glass slides in Mowiol® 4-88 (Sigma) mounting medium containing 1mg/ml p-phenylenediamine (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... The flies were then transferred into vials containing Drosophila instant food (Formula 4-24 Instant Drosophila Medium, Carolina Biological Supply Company, USA) containing 5% H2O2 (Sigma, H1009), or vials containing Drosophila instant food only as a control ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA)) ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS (1x PBS, 5 min) and then fixed in 4% PFA (Paraformaldehyde, Sigma Aldrich, Dorset, UK) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... female mice between 4 and 16 weeks old were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PMSG, Sigma, G4877) and 48 hours later ...
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing the complexes were incubated for 1 hr at 4°C with 10 mM N-hydroxysulfosuccinimide and 5 mM EDC (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were incubated overnight at 4°C in TBS Tween (TBST) plus 5% powdered milk and probed with either anti-DNP (Millipore; S7150) or anti-β-actin (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Cell Biology 2019Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Sigma-Aldrich) for 5-30 min at RT ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were applied overnight at 4 °C.” Sections were washed in 0.1M PBS and blocked with 5% Chemiblocker (Millipore, Darmstadt,Germany) comprising 0.5% Triton X-100 and 0.05% NaN3 in PBS at pH=7.4 ...
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bioRxiv - Genetics 2020Quote: ... scales were mounted on a microscope slide containing 10 % (w/v) Mowiol® 4-88 (Sigma-Aldrich cat# 9002-89-5) in 25% (v/v ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: PKO cells transfected with Flag-tagged OGT before cultured in 5 mM glucose were collected and incubated overnight at 4°C with ANTI-FLAG M2 affinity gel (Sigma, A2220). Immunoprecipitated complexes were eluted and stained with Coomassie blue ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were cultured for one week in 4 ml YNB media with a final concentration of 5 mM Nicotinamide (Sigma I17451). For copper treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... alongside a topical treatment for the tail skin (tails were dipped for 5 seconds in 25 mg/ml 4-hydroxytamoxifen (Sigma H6278) in DMSO) ...
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bioRxiv - Bioengineering 2022Quote: ... hBMSCs were frozen at passage 4 with 5*106 cells/ml in freezing medium containing fetal bovine serum (FBS, BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Cell Biology 2022Quote: ... followed immediately by an equal volume of a freshly-prepared 5 mg/mL solution of 4-hydroxy-α-cyano-cinnamic acid (Sigma) in 50% aqueous (v:v ...
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bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... The fused embryos were treated with 5 μmol/L ionomycin for 4 min and then incubated in mSOF medium (Sigma, USA) containing 2 mmol/L 6-Dimethylaminopurine(6-DMAP ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
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bioRxiv - Bioengineering 2022Quote: ... hBMSCs were frozen at passage 4 with 5*106 cells/ml in freezing medium containing fetal bovine serum (FBS, BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Neuroscience 2024Quote: Mice aged 3 to 5 months were deeply anesthetized and transcardially perfused with 50mL PBS followed by 50mL 4% (w/v) paraformaldehyde (Sigma-Aldrich) in PBS ...
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bioRxiv - Immunology 2024Quote: ... gut permeability was evaluated by gavaging mice that were fasted for 4 h with 5 mg FITC-dextran 4000 (Sigma -Aldrich) in 200 μl PBS ...
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bioRxiv - Biochemistry 2023Quote: ... DHEA sulfate (5-androsten-3β-ol-17-one-3-sulfate) and testosterone (4-androsten-17β-ol-3-one) were purchased from Sigma Aldrich. 11β-hydroxyandrostenedione (11β-hydroxy-4-androstene-3,17-dione) ...
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bioRxiv - Pathology 2023Quote: ... Freshly resected left ventricular tissue (∼2.5-4 mg per sample) was pulled into fibers and permeabilized in the presence of saponin (50 µg/mL, Sigma, 47036) 30 min at 40C in BIOPS buffer containing the following ...
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bioRxiv - Molecular Biology 2023Quote: ... Half of the beads were then incubated for 1 h at 4°C in 50 µl of 5 mM free CoA (C4282, Sigma-Aldrich) (dissolved in H2O) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich] ...
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bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed in 1X PBS for 5 min and coverslipped with mounting media (50% glycerol – Sigma, G5516 and 4% propyl gallate – Sigma, 2370).
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bioRxiv - Microbiology 2023Quote: HLCs were infected at day 18 of the differentiation protocol at an MOI of 5 infectious units (IU) in the presence of 4% polyethylene glycol 8000 (PEG, Sigma-Aldrich) and 1.5% DMSO (Sigma-Aldrich) ...