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Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... clone GS-6 (Millipore-Sigma #MAB302) was used at 1 ...
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bioRxiv - Neuroscience 2023Quote: ... clone GS-6 (Millipore-Sigma #MAB302) was used at 1 ...
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bioRxiv - Plant Biology 2024Quote: ... and 10mM 6-benzylaminopurine (BAP; Sigma) were prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... 6 ng of trypsin (Sigma-Aldrich) was added and the samples were incubated overnight at 37 °C ...
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bioRxiv - Plant Biology 2019Quote: ... AGM was supplemented with vitamins (Duchefa M0409), cytokinin (200 nM 6-Benzylaminopurine), and IAA (3-indole acetic acid, 1 mM) and/or Trichostatin A (TSA, Sigma, T8552, final concentration 5 µM) or mock before pouring ...
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bioRxiv - Neuroscience 2021Quote: ... sample from the overnight cleavage step was concentrated to ∼5 mg/ml and injected into the Superose 6 Increase column equilibrated in SEC buffer with 1 mM DEET (Sigma Aldrich, CAS#134-62-3). Peak fractions were concentrated to ∼5 mg/ml and used immediately for grid preparation.
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bioRxiv - Genomics 2021Quote: HEK293T cells (ATCC® CRL-3216™) were seeded at a density of 5×105 cells/well in a 6-well culture plate (Sigma Aldrich, cat # CLS3516). The following day ...
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bioRxiv - Neuroscience 2024Quote: Sensitivity to cold was assessed immediately following testing for mechanical paw withdrawal thresholds by applying a droplet of acetone (Sigma-Aldrich; approximately 5-6 μL) from the end of a blunt one C.C ...
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bioRxiv - Cell Biology 2022Quote: ... FAM76B knockdown U937 cells were plated in 6-well plates (5×105 cells/well) and treated with 10 ng/ml PMA (Sigma-Aldrich, St. Louis, Missouri, USA) in association with 10 ng/ml LPS and 20 ng/ml hIFNγ (Sino Biological Inc. ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Bioengineering 2022Quote: ... electrodes were rinsed and placed in a 10 mM 6-mercapto-1-hexanol (6-MCH, Sigma-Aldrich) for 5 hours to passivate ...
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bioRxiv - Neuroscience 2021Quote: ... We then prepared a solution of 10 mg/mL 6-hydroxydopamine (6-OHDA; Sigma-Aldrich, H116-5MG) and 0.2% ascorbic acid in saline (0.9% NaCL ...
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bioRxiv - Neuroscience 2024Quote: ... We additionally prepared a solution of 10 mg/mL 6-hydroxydopamine (6-OHDA; Sigma-Aldrich, H116-5MG) and 0.2% ascorbic acid in saline (0.9% NaCL ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Both fins of each genotyped larva were dissected and transferred to a drop of Methyl Cellulose 3% (M0387, Sigma-Aldrich) on a slide for documentation using an Olympus SZX16 (Model SZX2-ILLB ...
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bioRxiv - Microbiology 2022Quote: ... embryos were mechanically dechorionated if necessary and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The bacterial suspension was then injected into the hindbrain ventricle of casper zebrafish larvae at 2 dpf ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250, mitomycin C, Sigma M4287 ...
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bioRxiv - Microbiology 2021Quote: ... the samples were dissolved in 50 μL of 20 mg/ml methoxyamine hydrochloride in pyridine for 1.5 h at 33°C followed by derivatization with N-Methyl-N-(trymethylsolyl)trifluoroacetamide (MSTFA, Sigma Aldrich) for 2 h at 35°C.
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bioRxiv - Neuroscience 2020Quote: ... The lesions were made by injecting N-Methyl-D-aspartic acid (10 μg/μL NMDA in 0.9% saline; Sigma, Germany) into the dorsoventral and mediolateral hippocampus at 8 sites bilaterally at a flow rate of 0.15 μL/min (see (Table 1 for coordinates) ...
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bioRxiv - Biophysics 2021Quote: D-glucose and 3-O-methyl-D-glucose powder for in vitro studies were obtained from Sigma-Aldrich (Milan, Italy). Solutions of D-glucose and 3OMG for in vitro studies were prepared in 10 mM phosphate-buffered saline (PBS 1X) ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... and incubated at 37° C for 90 minutes followed by addition of 20μL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934) and incubated at 60°C for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated at 37°C for 90 minutes followed by addition of 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert– Butyldimethylchlorosilane (Sigma 375934) and incubated at 60°C for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking Buffer (100 mM HEPES, pH 7.5 [Gibco], 1 mM EDTA [Sigma-Aldrich], 2.5% SDS [Sigma-Aldrich], 0.1% S-methyl methanethiosulfonate [Sigma-Aldrich]), Binding Buffer (100 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... The sample was then derivatized by adding 60 μL N-methyl-N-(trimethylsilyl)trifluoro acetamide (MSTFA) (Cat# 69479; Sigma-Aldrich) and incubating for 30 min at 37 °C ...
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bioRxiv - Physiology 2021Quote: ... from the “AngII + L-NAME” group (n=8) were given Nω-nitro-L-arginine methyl ester (L-NAME, Sigma-Aldrich) in drinking water (40 mg/kg/d ...
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bioRxiv - Neuroscience 2021Quote: ... Fatty acids were converted to fatty acid methyl esters (FAME) by heating with 14% boron trifluoride (BF3) in methanol (Sigma) and separated on capillary columns in an HP 5890 Series II GLC equipped with a flame ionization detector ...
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bioRxiv - Neuroscience 2021Quote: ... and 5mM of lysosomal damaging compound and LRRK2 activator (24) L-leucyl-L-leucine methyl ester (LLOMe, Sigma Aldrich #L7393) dissolved in DMSO ...
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bioRxiv - Genetics 2020Quote: Serial ten-fold dilutions of logarithmic yeast cells were spotted on fresh Synthetic Dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of Methyl methane sulfonate (MMS)(Sigma) and incubated at 30°C for three days ...
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bioRxiv - Cell Biology 2021Quote: ... sphere (1.5 μm – 3 μm)) coated with 8.45 mM poly(ethylene glycol)methyl ether thiol (Mn 800, Cat. No. 729108, Sigma Aldrich) solution for 2 h at room temperature were used ...
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bioRxiv - Cell Biology 2022Quote: ... Inhibitors and drugs (etoposide, trimethoprim, hydroxyurea, methyl methanesulfonate MMS, HIV protease inhibitors, E64, chloroquine and artemisinin) were from Sigma-Aldrich, Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... All larvae were then anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester at pH = 7.0 (Tricaine; Sigma-Aldrich, A5040), immediately de-yolked in de-yolking buffer (55 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds used in these studies are as follows: N-methyl-D-aspartate (NMDA; 1.5ul of 100mM in PBS; Sigma; M3262), recombinant mouse IL1β (200 ng/dose ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER scaffolds were manufactured by infiltrating cellulose paper strips (0.5 x 12 cm, Miniminit Products) with poly(methyl methacrylate) (PMMA, 120 kDa) dissolved in acetone (Sigma-Aldrich) at 0.175 g/mL with an Allevi Beta 1 3D printer ...
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bioRxiv - Neuroscience 2024Quote: ... the bound protein was visualized using 100 µl of Tetra-methyl-benzidine liquid substrate (TMB slow kinetic form, Sigma-Aldrich) at RT for 20 min ...
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bioRxiv - Genetics 2024Quote: Serial 10-fold dilutions of logarithmic yeast cells were spotted on fresh synthetic dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of methyl methanesulfonate (MMS) (Sigma) and incubated at 30°C for 3 days ...
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bioRxiv - Plant Biology 2024Quote: ... The tissue was blotted on tissue paper before weighing to remove excess water and then was transferred into a 1.5mL Eppendorf tube containing 1 mL of di-methyl sulfoxide (DMSO) (#D8418, Sigma Aldrich) and incubated in the dark at 65C with 300 rpm shaking for 45 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were treated with 1 ml pre-cooled (-20°C) mixture of methyl tert-butyl ether (MTBE, Sigma 306975), Optima LC-MS grade methanol (A456-212 ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then further incubated with 20 µL of N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS) (Sigma) at 60 °C for 60 min ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: Visualization of the accessible pool of cholesterol was performed after 30 min treatment with 10 mM methyl-beta-cyclodextrin (MβCB) in 1% lipoprotein-deficient serum (LPDS, Sigma #S5519) or 1% LPDS control ...
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bioRxiv - Immunology 2023Quote: ... HPLC grade methyl tert-butyl ether (MTBE), chloroform, ammonium fluoride (NH4F) (ACS reagent, ≥ 98%) and ammonium formate from Sigma-Aldrich or VWR ...
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bioRxiv - Immunology 2023Quote: ... 3 dpf larvae were anesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The corresponding concentration of bacteria was injected into the corresponding part of the zebrafish ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...
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bioRxiv - Biophysics 2024Quote: ... methyl viologen (MV) (catalog no. 856177-1G) and sodium hydroxide (catalog no. 30620-1KG-M) were obtained from Sigma-Aldrich. Methanol (catalog no ...
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bioRxiv - Molecular Biology 2023Quote: 2 x 106 hVSMCs were seeded in to tissue culture flasks and incubated with 15 mL of serum free complete medium (Cat. #311-500) for 24 hours under control or cholesterol treated (methyl-β-cyclodextrin-cholesterol mixture (5µg/ml, Sigma)) conditions using an established protocol35 ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were treated with 1 ml pre-cooled (-20°C) mixture of methyl tert-butyl ether (MTBE, Sigma 306975), Optima LC-MS grade methanol (A456-212 ...
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bioRxiv - Cell Biology 2024Quote: ... Fully grown oocytes were collected 48 hours after injection and placed in M2 medium containing 200 μM 3-isobutyl-1-methyl-xanthine (IBMX, Sigma) at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Reagents used in this study were from the following sources: Leu-Leu methyl ester hydrobromide (LLOMe, L7393) from Sigma Aldrich. Lysotracker Red DND-99 (L7528 ...
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bioRxiv - Bioengineering 2024Quote: ... The instrument was calibrated against a poly(ethylene glycol) methyl ether standard (Sigma Aldrich, Average Mn = 2,000 g mol-1) prepared under the same conditions with DCTB matrix.
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bioRxiv - Developmental Biology 2024Quote: ... and contained 800 μl culture media (Leibovitz’s L15 Medium, no phenol red, Thermo Fisher 21083027; 50 ng/μl Protein A; 0.01% Methyl Cellulose, Sigma 274429). Surface conditions were critical to successful cultures ...