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Citations for Millipore Sigma :
3351 - 3400 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Beads were then washed four times with lysis buffer and eluted in 2x Laemmli buffer containing 1% 2-mercaptoethanol (Sigma) by heating at 95°C for 3min.
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bioRxiv - Neuroscience 2021Quote: ... The labeled samples were washed 3 times in PBT (10 min each) and incubated with DAPI (Sigma, cat. N. D9542) (5μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times and incubated with a 1:10,000 dilution of horseradish peroxidase-conjugated anti-rabbit antibodies ((Sigma-Aldrich) for 1-2 h ...
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bioRxiv - Neuroscience 2020Quote: ... Basal oxygen consumption was measured six times followed by three measurements after each addition of 1 µM of oligomycin A (Sigma), 2 µM carbonyl cyanide 4-(trifluoromethoxy ...
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bioRxiv - Microbiology 2021Quote: ... were taken from the reaction vessels at the desired contact times and added to tubes containing 9 mL deionized water with 0.1% (wt./vol.) sodium thiosulfate (Sigma Aldrich) to immediately neutralize residual chlorine ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were then rewashed three times with PBST before the addition of 3,3’,5,5’-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, UK) and incubated for 12 minutes in the dark at RT ...
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bioRxiv - Genetics 2022Quote: ... Slides were then rinsed in washing buffer five times for 3 min each and nuclei were stained with 4lr,6-diamidino-2-phenylindole (DAPI, cat # D-1388, Sigma) at a concentration of 1 μg/ml for 10 min at RT ...
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bioRxiv - Pathology 2022Quote: ... Slices were washed three times with cold dissociation solution then transferred to a trypsin inhibitor/bovine serum albumin (BSA) solution (1 mg/ml; Sigma) in cold HBSS (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: All MPTP experiments used the acute regimen consisting of four times of intraperitoneal injection of MPTP-HCl (M0896, Sigma Aldrich, 2 mg/mL in saline ...
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bioRxiv - Bioengineering 2019Quote: Substrates were placed into 6-well plates and both chambers were washed three times with 50 mM HEPES pH 8.5 (Sigma H3375). The polyacrylamide was activated for ECM protein coating by covering the substrates with 500 μL of 0.5 mg/mL sulfo-SANPAH (Proteochem c1111 ...
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bioRxiv - Bioengineering 2019Quote: ... PET slips were then washed with ethanol three times and air dried before being submerged in ethylenediamine (Sigma-Aldrich E26266) for 45 minutes in glass petri dishes ...
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bioRxiv - Neuroscience 2019Quote: ... the samples containing the purified CNTNAP2-ecto and the vehicle control were dialyzed 3 times using 100 kDa spin filters (Millipore) to remove the glycine and substitute it with a TBS solution.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed three times with 1x PBS and incubated for 5 minutes in 300 nM 4′,6-diamidino-2-phenylindole (Sigma) before a final 1x PBS wash ...
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bioRxiv - Immunology 2019Quote: ... The pellets of the lysates were washed with PBS for three times and cross-linked using fresh DSS (2 mM, Sigma) at 37°C for 30 min ...
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bioRxiv - Systems Biology 2020Quote: ... at room temperature and rinsed 2 times quickly with 1%BSA-1XPBS by using a 0.22 μm Millex 33mm PES sterile filter (SLGSR33RS, Sigma-Aldrich) at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... The identification amino acids was based on retention times and m/z values obtained from amino acid standards (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... Slides were incubated for 1 h at room temperature in a humid chamber and then dried under a hood and then washed two times for 10 min with 0.4% H2O/Photoflow (Sigma-Aldrich). Slides were stained immediately or dried and stored at −20 °C ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... Additional DNA digestion was performed two times after binding of RNA to RNA-column with 4 units DNase (Sigma Aldrich) in according reaction buffer for 20 min at RT ...
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bioRxiv - Pathology 2020Quote: ... 150 mM NaCl containing the corresponding detergents and bound proteins were eluted three times with 10 μL 0.5 mg/mL c-Myc peptide (Sigma M2435) in the corresponding detergent containing buffer ...
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bioRxiv - Pathology 2020Quote: ... The ELISA plates were washed five times with wash buffer (PBS 0.05% Tween 20) and blocked with 100 μL/well 5% FBS (Sigma, F9665) in PBS 0.1% Tween 20 for 1 h at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... the supernatant was collected and then 4 fractions of 4mL were concentrated 8 times using 10 kDa Amicon Ultra-4 10K Centrifugal filters (Millipore) after centrifugation (20 min ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 120 mL of water sample was also collected per time point and filtered through a 0.2 µm PES membrane filter (Millipore, Inc.) for shotgun metagenomic analyses ...
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bioRxiv - Microbiology 2020Quote: ... the bacterial suspension was discarded and tubes were gently washed 3 times with PBS and stained with 0.1% crystal violet (Sigma-Aldrich) for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... the conjugated antibody was washed several times with W-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 50kDa cut-off (Millipore-Sigma), and quantified using a NanoDrop spectrophotometer (280 nm) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fixed embryos were washed with 3%HCl/70%EtOH solution for 3 times and stained in 1% Victoria blue B (Sigma) dissolved in 3%HCl/70%EtOH overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Conditioned medium was concentrated ~40 times by Amicon Ultra-15 Centrifugal Filter Units with 30 kDa cutoff filter (Millipore, UFC903024), and then added to primary neuron cultures at 10 days in vitro (plated at 1.5 × 105 cells per well in a poly-D-lysine-coated 24-well plate) ...
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bioRxiv - Genomics 2021Quote: ... a frozen mouse cortex sample was subject to dounce homogenization (19 times with loose pestle followed by 4 times with tight pestle) in 12mL homogenization buffer (0.32M sucrose, 5mM CaCl2 (Sigma-Aldrich), 3mM MgAc2 ...
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bioRxiv - Microbiology 2021Quote: ... twice with 50 mM Tris-HCl pH 7.5 and three times with 100 mM Triethylammonium bicarbonate buffer (TEAB, Sigma-Aldrich) pH 8.5 ...
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bioRxiv - Biophysics 2019Quote: ... Cells were first rinsed three times with pre-warmed (at 37 °C) phosphate buffered saline (PBS, 806552-500ML, Sigma-Aldrich) and then fixed for 15 minutes at room temperature (RT ...
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bioRxiv - Immunology 2019Quote: ... The plates were washed three times with PBS (pH 7.4) and incubated with blocking buffer (2% bovine serum albumin (Sigma-Aldrich) in PBS-Tween 20 (0.05% ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS six times and 3,3′,5,5′-Tetramethyl benzidine (TMB) substrate (100 μL/well; Millipore, Billerica, MA) was used for detection followed by 0.5 M H2SO4 after 15 min (50 μl/well ...
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bioRxiv - Neuroscience 2019Quote: ... Slides were washed three times with PBS and blocked for 1 hour with 5% Normal Donkey Serum (NDS, Sigma-Aldrich) and 0.3% triton in PBS ...
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bioRxiv - Plant Biology 2019Quote: Small RNAs were prepared from untreated and UV-C treated plants (time points 0 and 24h) using the Tri-Reagent (Sigma), used for library preparation and sequencing by Illumina Hi-Seq (FASTERIS ...
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bioRxiv - Systems Biology 2019Quote: ... These were then spiked with synthetic heavy labelled analogues of the peptide targets and a retention time standard (MSRT1, Sigma) for scheduled selected reaction monitoring ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed 3 times for 2 min in 0.01 M PBS containing 0.2% Triton X-100 (Tx) (PBS-Tx; Sigma-Aldrich, T8787) and then were pre-treated with 3% H2O2 in PBS-Tx for 10 min ...
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Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: ... The bound peptides were eluted 3 times from the beads with 0.1% trifluoroacetic acid (TFA; Sigma-Aldrich, Saint Louis, USA). The eluted fractions were pooled together and vacuum-dried ...
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bioRxiv - Microbiology 2021Quote: ... the plates were washed 5 times with 200 μL of 1x PBST 100 μL of pNPP (Sigma, Cat. No. N2770) was added to each well ...
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bioRxiv - Biophysics 2021Quote: ... Reactions were incubated at 30°C and at the indicated times 2 μL were spotted on TLC PEI Cellulose F (Millipore) to stop the reaction ...
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bioRxiv - Cell Biology 2021Quote: ... plates were 4 times washed with PBST and incubated with 100 μL of 1: 2000 diluted Ultrasensitive Streptavidin-Peroxidase Polymer (Sigma). After further washing ...
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bioRxiv - Cell Biology 2021Quote: Live cell images showing the 2:2 segregation pattern and the time-lapse images were acquired with cells immobilized in a CellASIC Onix2 microfluidic device (Millipore) perfusing with fresh SPO media at a flow rate of 5kPa on a Nikon (Garden City ...
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bioRxiv - Genomics 2020Quote: ... An additional 5 μg/mL αF was then added at the same time as 5 μg/mL CPT (Sigma-Aldrich). The cells were pelleted for DNA extraction 2.5 h after adding the CPT ...
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bioRxiv - Molecular Biology 2021Quote: ... and induction of expression of respective gene product was carried out for indicated time durations using 400 ng/ml Doxycycline (SIGMA). HEK & HeLa cells were maintained in DMEM medium containing 2 mM L-glutamine and 10% heat-inactivated FCS as described earlier (Bose and Bhattacharyya ...
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bioRxiv - Molecular Biology 2020Quote: ... Wells were washed four times with PBS-T and 25 μL of prepared Immobilon Western Chemiluminescent HRP Substrate (Millipore #P90719) added to each well immediately prior to the plate being read on a Synergy Neo2 Plate Reader (BioTek Instruments) ...
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bioRxiv - Microbiology 2021Quote: ... and the cells washed three times in PBS and either lysed in 0.1% Triton–X100 (Sigma-Aldrich, St. Louis, MO) for enumeration of cell-associated/adhered bacteria ...
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bioRxiv - Microbiology 2021Quote: ... The ELISA plates were washed four times with PBST and then 100 μl of 2,000-fold diluted anti-strep-HRP (Millipore Sigma) was added into each well for another 1 hr at 37□°C ...
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bioRxiv - Genetics 2019Quote: ... the resin was washed three times in wash buffer (TBS containing 0.1% NP-40 and phosphatase inhibitor cocktails II and III, Sigma-Aldrich). Protein baits were eluted with Strep-elution buffer (2mM desthiobiotin in TBS) ...
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bioRxiv - Genetics 2019Quote: ... Hemocytes were washed (three times, 15 minutes each at 4 °C) in PBS before overnight staining with DAPI 1X (405 nm, Sigma) and Phalloidin 1X (488 nm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resulting multilamellar liposomes were extruded 11 times through two stacked polycarbonate membranes of 0.8 μm pore diameter (Whatman-Nuclepore, Sigma-Aldrich) using a custom-built high-pressure extrusion device or a syringe extruder (Avanti Polar Lipids). ...
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bioRxiv - Microbiology 2022Quote: Apoptosis was induced by rm-CSF1 deprivation (from 30 ng/ml to total removal) for different periods of time or by the addition of 5μg/ml Actinomycin D (AD, #A1410, Sigma-Aldrich) for 16 hours (Lisi et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The coverslips were washed three times with PBS 1X at room temperature and fixed in 1 mL/well of PFA 4% (Sigma) under the chemical hood for 10 minutes ...