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Citations for Millipore Sigma :
3201 - 3250 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... virus-containing supernatants were used to transduce A2EN cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich). Cells were co-transduced with lentiviral vectors encoding the two distinct sgRNAs that target the same gene ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl [pH 8] and 0.1% (w/v) SDS) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) and frozen at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... 7 mL of collagen solution was mixed with 800 μL Phenol Red (Sigma-Aldrich, ACS Cas.# 143-74-8) to act as pH indicator ...
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bioRxiv - Cell Biology 2023Quote: ... and the medium was replaced with viral supernatant diluted 1.5-fold with 8 μg/mL polybrene (H9268; Sigma-Aldrich). Two days later ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Target cells were transduced with 0.5 mL of viral supernatant in 3 mL of total medium supplemented with 8 µg/mL polybrene (Merck Sigma). 2 to 4 days post transduction ...
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bioRxiv - Cell Biology 2023Quote: ... and the resulting pellets were resuspended in a buffer consisting of 100 µl 8 M Urea (Sigma-Aldrich, #U1250), 100 mM TEAB (triethylammonium bicarbonate ...
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bioRxiv - Biochemistry 2023Quote: ... EA-nap suspension (1 mL) was placed into an ultrafiltration centrifuge tube (interception molecular weight: 8 kDa; Millipore, Ireland), and centrifuged at −4℃ for 20 min at 12,000 r/min ...
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bioRxiv - Microbiology 2023Quote: ... approximately 8 L of seawater was filtered onto 0.22-μm Sterivex-GP pressure filter units (Millipore, Billerica, MA, USA). The filters were immediately soaked in 2 mL of RNAlater Stabilization Solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Viral-containing supernatants were cleared of cellular debris by 0.45 μM filtration and mixed with 8 μg/ml polybrene (Sigma). Target cells were exposed to viral supernatants for two 24 h periods before being washed ...
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bioRxiv - Cell Biology 2024Quote: ... and plated in Induction Medium (Essential 8 Medium,10 μM Chroman1, MedChemExpress, and 2 μg/mL Doxycycline hydrochloride, Sigma) at 400,000 cells per well on double-coated [hESC-qualified Matrigel Matrix and Poly-L-Ornithine (Sigma)] 10 cm tissue-culture treated plates ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins from the purified PB or pre-sorting fractions were dissolved in a 8 M urea solution (Sigma-Aldrich) before trypsin digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries and testes were ground using RNase-free 1.5 mL pestles (SLS) in 500 mL lysis buffer B per gradient [50 mM Tris-HCl pH 8 (Sigma), 150 mM NaCl ...
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bioRxiv - Genetics 2024Quote: ... The powder obtained was added to 100 μL chondroitinase ABC solution (50 mM Tris HCl pH 8, 50 mM sodium acetate, 0.1 mU/μL chondroitinase ABC (Millipore)) and incubated in a thermomixer set to 37 °C and 1,500 rpm for 6 h ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Immunology 2021Quote: Degranulation and cytokine production of NK cell subsets were determined after cytokine stimulation o/n with IL-12 (10ng/ml, Sigma Aldrich, USA) and IL-18 (5ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphorylated-tau 181 (p-Tau) were measured according to manufacturer specifications (Luminex xMAP technology; EMD Millipore, Chicago, IL, USA: HNABTMAG-68K), modified by a 1:10 dilution of CSF ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2019Quote: Synaptoneurosome samples and a protein ladder were separated on 4-20% SDS-PAGE gels (Pierce, Rockford, IL) and transferred to polyvinyldenine fluoride (PVDF) membranes (Millipore, Billerica, MA). The blots were blocked in PBS containing 0.05% Triton-x (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... conjugated anti-histidine was added and color developed by addition of and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St. Louis, MO, USA). Plates were read at an OD of 450 nm with a SpectraMax Plus plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Immunology 2022Quote: ... BLaER1 cells were trans-differentiated for 5-6 day in medium containing 10 ng/mL IL-3, 10 ng/mL M-CSF (MPI of Biochemistry, Munich) and 100 nM β-estradiol (Sigma-Aldrich) at a concentration of 7 × 104 cells/well in 96 well plates ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasma was stored at −30°C for analysis of leptin and insulin (CrystalChem Inc., Chicago, IL, USA) and triglyceride content (Roche triglyceride reagent, Sigma glycerol standard).
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bioRxiv - Microbiology 2024Quote: ... the maintenance and expansion of T cells was done by adding medium with 10 ng/ml interleukin-2 (IL-2, Stemcell) (10 ng/ml) and for activation Lectin from Phaseolus vulgaris (red kidney bean, PHA) (Sigma, 1µg/ml).
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bioRxiv - Genetics 2023Quote: Tissue lysates in RIPA buffer containing protease and phosphatase inhibitors as above were used for ELISA measurements of IL-1 beta (#RAB0275, Sigma Aldrich, GmbH) and IL-6 (#RAB0309 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with bovine serum albumin (0.5%) before labeling with anti-STIM1 monoclonal antibody (1:50 dilution; 30 min; Sigma-Aldrich, Rockford, IL). Cells were then washed before the addition of Alexa Fluor 488-conjugated goat anti-mouse IgG (1 µg/ml dilution ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were incubated with 0.5 µg/mL of chicken anti-human C1qA (Sigma-Aldrich), followed by incubation with PE-conjugated donkey F(ab’)2 anti-chicken (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2021Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of a lentiviral vector (for human shSUV39H1, Sigma, TRCN0000157251 ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Human HEK293T embryonic kidney cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human neutrophils were purified from fresh peripheral blood by Histopaque®-1119 (Millipore Sigma) and subsequent discontinuous Percoll® gradient centrifugation (102) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with primary antibodies (mouse anti-human synuclein, Sigma S5566 (clone SYN211) for human alpha-synuclein ...
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bioRxiv - Biochemistry 2020Quote: ... full length human PCNA (accession no. NM182649) was cloned into pETDuet-1 MCS1 (Novagen) Amp+ to obtain 6× His N-terminally tagged protein ...
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bioRxiv - Developmental Biology 2019Quote: ... S4 are anti human Tcf7l2 (N-20, SCBT) and anti-gamma tubulin (T9026, Sigma) HRP coupled secondary antibodies (1/2,000 ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from human islets using Trizol (Ambion, UK or Sigma Aldrich, Canada). To clean remaining media from the islets ...
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bioRxiv - Immunology 2019Quote: ... incubated with alkaline phosphatase-conjugated goat anti-human IgG (1 μg/mL, Sigma-Aldrich) for 1 hr at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Monocyte-derived macrophages were grown in RPMI 1640 supplemented with 10% human serum (Sigma) with added L-Glutamine ...
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bioRxiv - Developmental Biology 2019Quote: ... Human cells were detected using directly Cy3-conjugated anti-HNA (Millipore, MAB1281C3, 1:100). After washing in TBS ...
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bioRxiv - Pathology 2019Quote: ... CG (22 ng/μL in PBS) was isolated from human leukocytes (Sigma-Aldrich, Germany). Purified native lubricin (SF Lub ...
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bioRxiv - Immunology 2020Quote: ... pMCPECs media supplemented with 5μg/ml human insulin (Sigma Aldrich, St Louis, MO, USA), 10ng/ml hEGF (Peprotech ...
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bioRxiv - Molecular Biology 2019Quote: RAW264.7 mouse macrophage or C32 human melanoma cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2019Quote: Morphogenesis was induced by the addition of recombinant human HGF (product H1404, Sigma-Aldrich) and conditioned media from NIH/3T3 cells (product CRL-1658 ...
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bioRxiv - Immunology 2019Quote: Human cytokine quantification was performed using a custom 31-plex Luminex panel (EMD Millipore) containing the following analytes ...
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bioRxiv - Immunology 2019Quote: ... wells were incubated with HRP-conjugated anti-human IgG or IgM antibodies (Sigma-Aldrich) diluted 1:500 in PBS/BSA for 90 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... and DENV-specific antibody binding quantified using anti-human IgG HRP (Sigma-Aldrich, SAB3701362). Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Pathology 2021Quote: Cell culture inserts were coated with human placental type IV collagen (Sigma-Aldrich, C7521). CRC expanded cells were seeded at 2.5 × 105 cells on 12-mm Snapwells (Corning Costar ...
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bioRxiv - Developmental Biology 2021Quote: ... they underwent intraperitoneal injection of 20 IU human chorionic gonadotropin (9002-61-3, Sigma) (27) ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeding on 16.7 µg/ml human plasma fibronectin-coated plates (Merck Millipore, FC010). After 48h ...
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bioRxiv - Immunology 2021Quote: ... human endometrial cells were incubated for 4 hours with monensin and Brefeldin A (Sigma) in the presence or absence of 100 ng/ml LPS (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... and DENV-specific antibody binding quantified using anti-human IgG HRP (Sigma-Aldrich, SAB3701362). Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL ...