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Citations for Millipore Sigma :
3051 - 3100 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Fresh or cryopreserved primary adult human hepatocytes (BeCytes Biotechnologies, BiolVT or Sigma-Aldrich) were resuspended in hepatocyte spheroid medium (HSM) ...
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bioRxiv - Neuroscience 2024Quote: Human spinal cord samples were homogenized with a dounce homogenizer (Sigma Aldrich, D8938) in 1mL nuclei lysis buffer using the Nuclei Pure Prep Nuclei Isolation Kit (Sigma Adlrich ...
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bioRxiv - Cell Biology 2024Quote: ... primary human skeletal muscle cells were seeded and differentiated on laminin (Sigma Aldrich) coated 4-well Nunc Lab-Tek II Chamber Slides (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO, USA). The cells were cultured using Ex Plus in both the basolateral and apical compartments for 1 week ...
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bioRxiv - Microbiology 2024Quote: Purified human PMNs were incubated with phorbol 12-myristate 13-acetate (PMA) (Sigma) 100nM for 4h ...
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bioRxiv - Microbiology 2024Quote: ... Coverslips with C3bi-coated beads were incubated with rabbit anti-human IgM (Sigma) at 1:250 ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Target cells were then transduced overnight with the filtered virus in the presence of 8 μg/mL polybrene (Millipore).
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bioRxiv - Biophysics 2020Quote: The fibrillization kinetics of p53 R248Q was monitored at 37°C with 1-anilino-8-naphthalenesulfonate (ANS, Sigma Aldrich) assay19–21 ...
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bioRxiv - Biophysics 2020Quote: ... the desired lipid mixture is applied to indium tin-oxide coated glass slides (8-12 Ω/sq, Sigma-Aldrich), as a chloroform solution (15 μL ...
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bioRxiv - Cell Biology 2020Quote: Cells were incubated in a solution containing 8 mg of naphthol AS-MX di-sodium phosphate salt (Sigma-Aldrich) in 500 µL of NN-dimethylformamide ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured for 8 hours in fresh complete medium before adding 50 ng ml−1 nocodazole (Sigma-Aldrich). After 12 hours ...
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bioRxiv - Cell Biology 2020Quote: ... The CFs were incubated with PGK-dsRed lentivirus and 8 μg/mL of polybrene hexadimethrine bromide (Sigma Aldrich, USA) for 24 hours at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 µl of total RNA (2.5µg/µl) were added to 20 µl of “glyoxal mix” (60% DMSO, BTPE 1.2X, 5% glycerol, 8% glyoxal solution (Sigma) and 40 µg/ml Ethidium bromide) ...
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bioRxiv - Biophysics 2021Quote: ... cells were washed with Dulbecco’s phosphate buffered saline and detached with trypsin-EDTA (7–8 min, 37 °C, 5% CO2) (Sigma). Cells were labelled with CMDiI (Thermofisher UK ...
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bioRxiv - Immunology 2020Quote: ... mosquitoes were kept at 26.0°C (± 0.5°C) in an ultrasonic humidity cabinet and provided with 8 % Fructose and 0.05 % 4-Aminobenzoic acid (both Sigma) feeding solution from this point forward ...
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bioRxiv - Immunology 2022Quote: ... TOs and DOs were plated directly onto 15μL of a Matrigel coating in each well of an 8-well chamber slide (Millicell, EZslide, Millipore) or onto 20μL of Matrigel coating in 24-well plates and cultured for 5-7 days prior to infection ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were separated by 8% or 10% Tris-Glycine-SDS page gels for 1 h and transferred on PVDF membrane (0.45 um, Millipore). Then blocked the membrane with 5% skimmed milk in TBS-T buffer (20 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... 8-16-weeks-old mice were treated with tamoxifen-containing water (1 mg/ml) (T5648, Sigma-Aldrich, Darmstadt/Germany) to induce Cre recombinase activity ...
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bioRxiv - Genomics 2020Quote: ... cells were lysed on ice in 5 ml lysis buffer (10 mM Tris-HCl, pH 8, 10 mM NaCl, 0.2% Igepal NP-40 (Sigma), 1× cOmplete protease inhibitor (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... 8 µg of antibody (anti-acetyl-histone H4, Millipore Sigma 06-598; anti-acetyl-histone H2A.Z, Millipore Sigma Abe1363) was then added and tubes were rotated overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were permeabilized in 1ml lysis buffer (10mM Tris–HCl pH 8, 10mM NaCl, 0.2% NP-40, protease inhibitor cocktail (Sigma) for 15 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... GST (clone 8-326, MA4-004 from Thermo Fischer, 1:1000) and Cyclin B (C8831 from Sigma, 1:1000). The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad ...
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bioRxiv - Physiology 2019Quote: On average 8 mg of muscle tissue was pulverized in liquid nitrogen and mixed with the lysis buffer containing protease inhibitor cocktail (8 M Urea, 2M Thiourea, 4% CHAPS, 1% Triton X-100, 50 mM Tris, pH 8.5 (Sigma)) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were subsequently fixed in 8% formaldehyde and stained using the Alkaline-Phosphatase kit (86R 1KT, Sigma-Aldrich, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... ~2×108 cells were collected and lysed in a hypotonic buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Sigma) for 20 minutes ...
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bioRxiv - Neuroscience 2019Quote: Fixed brains were washed in phosphate buffered solution and embedded in 8% (w/v) agarose (low melting point, Sigma) in water ...
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bioRxiv - Microbiology 2019Quote: ... Phage pellet was resuspended in 2 ml of SM buffer (100 mM NaCl, 8 mM MgSO4, 50 mM Tris pH 7.5 (Sigma) and treated with 0.25 μg of RNAse A (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... and resuspended in 100 μl of SM buffer (100 mM NaCl, 8 mM MgSO4, 50 mM Tris pH 7.5 (Sigma)) ...
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bioRxiv - Cancer Biology 2020Quote: ... LPS stimulated B-cells were transduced by retroviral pool spin infection in 8 μg/μl of polybrene (Sigma-Aldrich). Each retroviral pool contained vectors expressing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 70 μl of 8 M urea (Fisher Cat #: U15500) in 50 mM ammonium bicarbonate (Sigma Cat#: 09830-500G), and stored at −80°C before proteomics analysis ...
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bioRxiv - Genomics 2019Quote: ... cells were collected at day 8 and RNA was extracted using GenElute Mammalian Total RNA MiniPrep Kit (Sigma #RTN350). cDNA was synthesized using oligodT primers using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific #18064014) ...
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bioRxiv - Neuroscience 2019Quote: ... 8-week-old male and female mice were anesthetized with 4% isoflurane and injections of 1.2% avertin (Sigma-Aldrich). Mice were perfused with 10 mL of ice-cold cold N-methyl-D-glucamine (NMDG)-based cutting solution ...
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bioRxiv - Cell Biology 2020Quote: ... 3-Isobutyl-1-methylxanthine (15879) and 8-Bromoadenosine 3’,5’-cyclic monophosphate sodium salt (B7880) were purchased from Sigma. Phosphodiesterase inhibitor Tocriset containing Milrinone ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with SEC61-GFP expression vector in 8 well chamber slide (Millipore Sigma, Millicell EZ SLIDE) and ...
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bioRxiv - Neuroscience 2021Quote: ... to assess protein concentration and then solubilized in 4x loading dye (250 mM Tris-HCl pH 6.8, 40% glycerol, 0.008% bromophenol blue, 8% SDS; all from Sigma-Aldrich). All samples were boiled at 65°C for 10 minutes and then equal amounts of each sample were separated using SDS-PAGE and subsequently blotted on nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... then triturated and plated on poly-L-lysine coated 8-well glass chambers (Sigma, P2636, Cellvis, C8-1-N) at a density of 25,000 cells/well in 5 % horse serum (Gibco 26050-088 ...
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bioRxiv - Cell Biology 2021Quote: ... retroviral particles were produced as described (Guimbal et al., 2019) and RAW264.7 cells were infected for 24h in 8 μg/ml polybrene (Sigma). Alternatively ...
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bioRxiv - Biochemistry 2021Quote: ... These were used to transduce A549 cells in the presence of 8 μg/mL of hexadimethrine bromide (Polybrene, Sigma). Following 24 hours of transduction ...
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bioRxiv - Microbiology 2021Quote: ... Samples were kept in dark and cold conditions while prefiltered by gravity through 10 μm nylon mesh (KC Denmark) and then onto 8-12 47 mm 0.45 μm Durapore filters (Millipore) using a vacuum pump ...
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bioRxiv - Molecular Biology 2020Quote: ... Hypertrophic remodelling was induced in 8-10 week old male mice animals by administration of isoproterenol (Iso, Sigma-Aldrich) at 10 mg/kg/day for one week via osmotic mini-pumps (Alzet ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of bead solution was removed and replaced with 200 μL of phenol:chloroform:isoamyl alcohol (Sigma; pH 7-8) before the stool was added ...
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bioRxiv - Cell Biology 2020Quote: ... The washed beads were resuspended in the same buffer containing HRV 3C protease (8 units/ml in final; Novagen) and incubated without agitation (4°C ...
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bioRxiv - Microbiology 2021Quote: ... 1.2×108 cells were transduced with lentivirus-packaged sgRNA library at MOI 0.3 in the presence of 8 μg/ml polybrene (Sigma) to achieve ~500-fold overrepresentation of each sgRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with selection medium (SM, 8 mM L-glutamine and phenol red, with 0.5 mg/mL G-418, Sigma). Cell pools were maintained at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... MTX (Applied Biosciences) Transduction of JJN3 MMCL was performed in the presence of 8 μg/ml polybrene (Sigma, UK). On day 3 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were dissolved in 100% methanol doped with NEM [8 mM,1 mg/mL] (Sigma-Aldrich, cat. no. E1271) (110) ...
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bioRxiv - Biophysics 2020Quote: ... Ovaries were disrupted using RNase-free 1.5mL pestles (SLS) in lysis buffer (50 mM Tris-HCl pH 8 (Sigma), 150 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 8-week-old rats received a single intravenous dose of 60 mg/kg streptozotocin (STZ, Sigma Chemical, MO, USA) diluted in STZ-vehicle (cold citrate buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transduced in DMEM medium supplemented with 10 mM HEPES and 8 μg/ml of Polybrene (Sigma H9268) immediately prior to the conditioned medium being added.