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Citations for Millipore Sigma :
3101 - 3150 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... 10% glycerol) to a conductivity of 85 uS/CM and loaded onto an 8-mL SP-Sepharose column (Sigma). Cdc6 was eluted in 20-mL steps of buffer A containing either 0.2 M ...
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bioRxiv - Neuroscience 2022Quote: ... First 400 µL of 100 mM pentafluorobenzyl bromide (PFB-Br, CID: 1765-40-8, Sigma-Aldrich, Catalog number: 101052), prepared in acetonitrile (Fisher Chemical ...
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bioRxiv - Genetics 2022Quote: Fipronil (CAS# 120068-37-3) and Spinosad (CAS# 168316-95-8) were both obtained as analytical standards from Sigma. Insecticide bioassays were performed as described previously [20] ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated by SDS-PAGE with 8% polyacrylamide gel and transferred to PVDF membrane (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk in Tris-buffered saline (20 mM Tris [pH8.0] ...
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bioRxiv - Bioengineering 2022Quote: ... PeproTech], 10 ng/mL keratinocyte growth factor [KGF; PeproTech], 50 nM dexamethasone [Sigma], 0.1 mM 8-Bromo cAMP [Sigma] ...
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bioRxiv - Biochemistry 2021Quote: ... Ovaries and testes were disrupted using RNase-free 1.5 mL pestles (SLS) in lysis buffer A (50 mM Tris-HCl pH 8 (Sigma), 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM PMSF and 0.01% Triton X-100) with 8 μl of SIGMA FAST protease inhibitor cocktail (Sigma-Aldrich). The pellets were then sonicated at 30% amplitude for 10 sec with 10 sec intervals on ice and were then centrifuged for 30 min at 4°C and 14,000rpm ...
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bioRxiv - Microbiology 2021Quote: ... Buffer exchange into TBS pH7.4 and 1 mg/ml Amphipol 8-35 using Amicon Ultra-4 centrifugation filters with a 100kDa cutoff (Millipore). The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... and transported back to the laboratory for immediate filtering (i.e. within maximum 8 hours after retrieval) through a 0.2 µm polyethersulfone filter (Steritop; EMD Millipore). Filters were frozen at −20 °C until DNA extraction ...
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bioRxiv - Plant Biology 2021Quote: ... The 50 µl pre-washed with washing buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 0,1% Tween-20) M2 anti-FLAG magnetic beads (Sigma) was added to supernatant ...
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bioRxiv - Molecular Biology 2020Quote: ... KCl 5 mM, glucose 8 mM, HEPES 10 mM, MgCl2 1.2 mM, and CaCl2 1.5 mM; all supplied by Sigma Aldrich) containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM ...
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bioRxiv - Neuroscience 2021Quote: ... at a plating density of 350 cells/well in 8-well chamber slides pre-coated with 100μg/ml poly-D-lysine (#A-003-M, Sigma). CNS myelin extracts (CME ...
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bioRxiv - Biophysics 2021Quote: ... and an oxygen scavenging system45,46 (final concentrations in buffer: 8 mg/mL glucose, 0.15 mg/mL catalase (from Aspergillus niger: Millipore Sigma, formerly EMD Millipore ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse embryos were cultured until the 8-cell stage in KSOM medium supplemented with 0.4% bovine serum albumin (Millipore), after which the medium was changed to Cook Blasto ® (Cook Ireland Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently scraped with ice cold RIPA buffer (50 mM Tris pH 8, 150 mM NaCl, 0.5% deoxycholate, 0.1% SDS, 1% NP-40; all chemicals from Sigma Aldrich) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Cell Counting Kit-8 (CA1210) and D-Luciferin (L6882) were all purchased from Sigma-Aldrich (Saint Louis, MO, USA). Lipofectamine2000 was purchased from Invitrogen (11668019 ...
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bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hours for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich). The iPSCs with a stably integrated human NIL under a tetracycline-inducible promoter were exposed to 2 µg/mL doxycycline in neuronal induction medium (NIM) ...
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bioRxiv - Developmental Biology 2019Quote: ... Denuded fail to fertilize M-II oocytes (n=8) were obtained 36h post-ovulation and cultured briefly (2-4h) in 50µl KSOM medium (Millipore) containing 0.4% BSA at 37°C ...
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bioRxiv - Immunology 2019Quote: ... Hem1-knockout Jurkat cells were seeded in 24-well plates in RPMI supplemented with 8 μg/mL Polybrene (Sigma) and 1 mL of Hem1-containing lentiviruses ...
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bioRxiv - Neuroscience 2019Quote: ... Adult zebrafish were assayed for response to a mixture of 8 amino acids (Ala, Cys, His, Lys, Met, Phe, Trp, and Val; 0.1 mM each in distilled water; Sigma). No stimulation was provided for the first 5 mins following which the amino acid mix was introduced in one side of the tank at the rate of 1 ml/min using a syringe pump ...
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bioRxiv - Genomics 2019Quote: ... were treated with ice-cold lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% TX-100, 0.5% IGEPAL-CA-630, Sigma; 5mM EDTA ...
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bioRxiv - Cell Biology 2019Quote: ... Medium containing virus was filtered through a 0.45 μm filter and used for HUVEC transduction with the addition of 8 μg/mL polybrene (Sigma). The target sequences used were as follows:
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bioRxiv - Biophysics 2019Quote: ... was diluted 1:100 to 8 units/mL in digestion buffer (50 mM Tris/HCl pH 8, 1 mM EDTA, 0.5% [v/v] Triton X-100, 1 M NaCl, Sigma) and incubated with the gels fully immersed in proteinase solution overnight at 23 °C ...
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bioRxiv - Microbiology 2019Quote: ... fumigatus from environmental samples 2 g of each sample was suspended in 8 ml of sterile distilled water with 0.85% NaCl (SIGMA, Sigma-Aldrich Chemie Gmbh ...
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bioRxiv - Microbiology 2019Quote: ... 16 g/ml streptomycin sulfate salt and 8 g/ml tebuconazole (PESTANAL analysis standard, Sigma-Aldrich AG Industriestrasse, Switzerland). These plates were incubated at 42°C and examined for growth after 72 hours ...
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bioRxiv - Neuroscience 2021Quote: ... adult flies upon eclosion were kept for 7-8 days before the experiment on food containing 0.5 mM all-trans retinal (Sigma).
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bioRxiv - Plant Biology 2020Quote: ... The washes were performed twice with Urea 8 M and then twice with 50 mM ammonium bicarbonate (Sigma-Aldrich) pH 8.5 ...
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bioRxiv - Zoology 2020Quote: ... We used standard dilutions of hen egg white lysozyme (0.5, 0.8, 1, 2, 4, 8, 10, 20, and 40 µg/ml; Sigma-Aldrich) to prepare a standard curve in each plate ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were solubilized in HU buffer (8 M urea, 5% SDS, 200 mM Tris-HCl pH 6.8, 20 mM dithiothreitol (DTT, Sigma) and bromophenol blue 1.5 mM) ...
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bioRxiv - Cancer Biology 2020Quote: ... and then transductions were carried out for 24 hours in the presence of 8 μg/mL polybrene (Sigma-Aldrich). Cells expressing fluorescent protein (GFP/RFP ...
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bioRxiv - Immunology 2019Quote: ... Age- and sex-matched adult mice (8-12 weeks of age) were orally administrated with 100 µl 1.33% sodium bicarbonate (Sigma), and then inoculated with 105 SD50 of murine rotavirus EC strain ...
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bioRxiv - Biophysics 2019Quote: ... The SU-8 master was silanized using vapor deposition of Trichloro(1H,1H,2H,2H-perfluoro-octyl) silane (Sigma) under vacuum ...
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bioRxiv - Biochemistry 2019Quote: ... the samples were buffer-exchanged against 150 mM aqueous ammonium acetate at pH 8 using Amicon Ultra-0.5 mL centrifugal filters (MWCO = 10 kDa; Millipore). MS measurements were performed in positive ion mode exactly as described before [23] ...
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bioRxiv - Genetics 2020Quote: ... NIH3T3 cells were seeded at 120,000 cells/ml and infected with 0.5 ml shScramble or shUBR5 retroviral supernatant in the presence of 8 mg/ml polybrene (Sigma). The media was changed after 24 hrs and cells were selected with 2 mg/ml puromycin 48 hrs post-infection.
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bioRxiv - Cell Biology 2021Quote: ... virus was mixed with 0.36 million cells in growth media supplemented with 8 μg/mL Polybrene (Sigma-Aldrich, H9268) and incubated overnight ...
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bioRxiv - Biochemistry 2021Quote: Male mice (8-9 wks) were treated with a single dose of tamoxifen (40 mg/kg i.p.; Sigma-Aldrich) or vehicle ...
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bioRxiv - Neuroscience 2020Quote: ... Surfaces of the Mylar film were coated by poly-D-lysine (0.1mg/ml in Trizma buffer, pH 8, Sigma) overnight ...
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bioRxiv - Synthetic Biology 2021Quote: ... medium was replaced with fresh growth medium (Essential 8 or Nutristem respectively) supplemented with Doxycycline hyclate (Dox, Sigma, D9891) at a final concentration of 0.8 μg/mL if required ...
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bioRxiv - Systems Biology 2021Quote: ... After the samples were blocked for 30 min in phosphate buffered saline (PBS, SH30256.01, HyClone) with 2% bovine serum albumin (BSA, 9048-46-8, Sigma), sections were incubated overnight at 4°C with primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... and osteogenic induction solution (10 mmol/L β-sodium glycerol phosphate, Sigma, USA; 10−8 mol/L dexamethasone, Sigma; 50 μmmol/L ascorbic acid ...
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bioRxiv - Physiology 2021Quote: ... resuspended in buffer S (50-mM Tris, pH 8, 150-mM NaCl, 25-mM imidazole, 0.5% CHAPS, and 0.25% Tween 20; Sigma-Aldrich) and lysed with a sonicator ...
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bioRxiv - Cancer Biology 2021Quote: ... SH-SY5Y cells were transduced with the viral supernatant in the presence of 8 μg/ml polybrene (Sigma-Aldrich) and 24 hr later selected using neomycin (G418 ...
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bioRxiv - Cell Biology 2020Quote: ... Group 2 (DN+CL, n=8): mice received surgical denervation of BAT and intraperitoneal injections of CL316243 (C5976; Sigma) at 1mg/kg for consecutive 14 days ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma] ...
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bioRxiv - Microbiology 2020Quote: ... The mice were boosted subcutaneously (s.c.) at day 8 with 50 μg of RBD-Fc plus Freund’s Adjuvant Complete (Sigma, USA) and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... small scalpel cuts were punctured into the mucosal surface of the tissue before cloning cylinders (8×8mm, Sigma-Aldrich) were adhered to the tissue using surgical glue (B Braun ...
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bioRxiv - Microbiology 2021Quote: ... and Vero E6 cells (ATCC CRL-1586) were grown in Dulbecco’s modified eagle’s medium (DMEM) supplemented with 8% fetal bovine serum (FBS, Sigma-Aldrich), 2 mM L-glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... filtered through 0.45 μm filters and used to transduce A549 cells in the presence of 8 μg/mL polybrene (Sigma). After 48 hours ...
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bioRxiv - Immunology 2021Quote: ... the cells were washed three times with warm PBS and incubated with fluorescein diacetate (8 μg/mL, Sigma-Aldrich) and propidium iodide (20 μg/mL ...
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bioRxiv - Immunology 2021Quote: 7-8 week-old B6J females were treated for 10 consecutive days with ampicillin (1 g/L; A0166 Sigma), vancomycin (500 mg/L ...