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Citations for Millipore Sigma :
3151 - 3200 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 6 h after the second thymidine release cells were transferred into medium containing 8 μM RO3306 (Sigma-Aldrich, SML0569) for 4 h ...
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bioRxiv - Cell Biology 2021Quote: 7-8 week-old age and sex-matched mice were used for treatments with arsenic (5ug/g, Sigma, #202673), pI:C (20 ug/g ...
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bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Immunology 2022Quote: ... The infected monolayers were left for 6-8 days in a 35°C incubator under 0.8% methylcellulose (Millipore Sigma) overlay (in EMEM medium with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... after which the cell culture medium was replaced with one containing 8 μM calcein-AM (Sigma-Aldrich, Cat# 17783) and ethidium homodimers (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: The virus was precipitated from the cell supernatant by adding 8 % (w/v) polyethylene glycol (PEG) 8000 (Sigma-Aldrich), incubating at +4 °C with gentle mixing for 3 h ...
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bioRxiv - Cell Biology 2022Quote: ... The gels were removed from the coverslips incubated overnight at 50℃ with 8 U/ml Proteinase K (Sigma-Aldrich) prepared fresh in digestion buffer (50mM TRIS ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transduced by adding retroviral supernatants in the presence of 8 mg/mL polybrene (H9268, Sigma Aldrich). The samples were centrifuged at 800g for 30 min and incubated at 37°C for 3h ...
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bioRxiv - Cell Biology 2022Quote: ... passed through a 0.45 µm nylon filter and used to transduce PSCs in the presence of 8 μg/mL polybrene (Sigma) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... EMM was placed onto 22 × 60-mm coverslips coated with 8 μL of lectins from Bandeiraea simplicifolia (Sigma-Aldrich). These coverslips were pre-equilibrated to the appropriate temperature of either 25 or 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... OGG1 sequence was PCR amplified from pPR71 (8) cloned into NdeI/EcoRI sites of the overexpression vector pET30a (Novagen). The recombinant pET30a-hOGG1 was used as a template to introduce Y203A and N149A/N150A (referred to as 2NA ...
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bioRxiv - Plant Biology 2022Quote: ... Then total proteins were resolved in 8% SDS-PAGE and transferred to immobilon-P membrane (MILLIPORE cat. No. IPVH00010). GFP antibody (trans #HT801) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Immunology 2022Quote: ... Protein extracts were loaded onto 8/15% polyacrylamide gels and then transferred onto a PVDF membrane (Millipore, Burlington, MA). Membrane was then blocked by incubating in 5% skim milk/TBS for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... or high pH medium supplemented with 0,01 μM 7-alpha,25-dihydroxycholesterol (7α,25OHC) (Sigma Aldrich, 64907-22-8) was added to the bottom of the chamber ...
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bioRxiv - Cell Biology 2022Quote: ... 6- to 8-week-old mice of the indicated genotypes were intraperitoneally injected with 2.5 ml 3% thioglycollate (T9032, Sigma). On day 4 after the thioglycollate injection ...
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bioRxiv - Cell Biology 2022Quote: ... filtered (45 μm) and used to infect MCF-10A cells in the presence of 8 μg/ml polybrene (Sigma). Clones of interest were selected using 2 µg/ml blasticidin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting solutions were fractioned by 8–20% precast polyacrylamide gels and transferred to 0.2 µm pore-size PVDF membranes (ISEQ00010, Millipore). Blot membranes were heated for 5 minutes in PBS for epitope retrieving before blocking ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected in lysis buffer (150mM NaCl, 50mM Tris-HCl pH 8, 1mM DTT, 0.5% NP-40, with protease and phosphatase inhibitors (Sigma).After 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in hypotonic lysis buffer (10 mM Tris/HCl pH 8, Roche protease inhibitors, 0.1% Triton X 100 Sigma) via dounce homogenization on ice ...
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bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hrs for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich).
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bioRxiv - Biochemistry 2022Quote: ... 8 mL of the modified live cell imaging solution supplemented with iodide and 20 μM forskolin (FSK, EMD Millipore; 20 mM HEPES buffer at pH 7.4 containing 40 mM NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... and peptide peak area ratios were generated against a spiked internal standard with bovine insulin (Sigma – 11070-73-8).
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bioRxiv - Cancer Biology 2022Quote: ... The ‘Positive Cell Detection’ function was used to identify Ki67+ and Ki67-cells using the following parameters: Nucleus Parameters (Requested pixel size 0.5 µm, Background radius 8 µm, Median filter radius 0 µm, Sigma 1.5 µm ...
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bioRxiv - Microbiology 2024Quote: ... [cyano-(3-phenoxyphenyl)methyl] 3-(2,2-dichloroethenyl)-2,2- dimethylcyclopropane-1-carboxylate (i. e. cypermethrin, pesticide analytical standard, CAS 52315-07-8, supplier: Sigma-Aldrich, purity ≥ 90.0% ...
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bioRxiv - Molecular Biology 2024Quote: ... Target cells were transduced on two consecutive days by supplementing the lentiviral supernatant with 8 μg/mL polybrene (Sigma). One day after the final transduction ...
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bioRxiv - Molecular Biology 2024Quote: hTERT-RPE1 p53-/- Cas9 expressing DD-HA-ER-I-PpoI were transduced with the lentiviral TKOv3 library at a low MOI (∼0.30) in the presence of 8 μg/mL polybrene (Sigma)43,44 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were crosslinked for 8 min at room temperature in 10ml PBS supplemented with 1% formaldehyde (Sigma, F8775). Cross-linking was quenched by adding glycine to a final concentration of 0.125 mM and incubating at room temperature for 1min ...
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bioRxiv - Genomics 2024Quote: ... the embryos (8-16 cell stage) were washed through M2 and then placed in drops of Tyrode’s solution (Sigma). After about 30 sec ...
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bioRxiv - Molecular Biology 2024Quote: ... the mice were subcutaneously injected with vitamin D3 (6 or 8 x 105 IU/kg; C1357, Sigma-Aldrich, USA) daily for three days (n=5-8 per group) ...
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bioRxiv - Cell Biology 2023Quote: ... which was diluted in culture medium supplemented with 8 µg/ml of protamine sulfate (Sigma-Aldrich, cat.no. P4505-1G). To generate stable cell lines ...
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bioRxiv - Plant Biology 2024Quote: ... Experiments assessing apoplastic pH were carried out as previously described (Barbez et al., 2017) using 8-hydroyypyrene-1,3,6-trisulfonuc acid trisodium salt (HPTS; Sigma-Aldrich). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... and the pellet was resuspended in 2 mL of ChIP buffer (33 mM Tris-HCl pH 8, 100 mM NaCl, 5 mM EDTA, 0.33% SDS, 1.67% Triton X-100, 1X protease inhibitor cocktail [Sigma; P8340]). Chromatin was sheared to approximately 200 bp to 500 bp fragments by sonication using a Bioruptor Plus (Diagenode ...
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bioRxiv - Genomics 2024Quote: ... Cells were resuspended in 1 ml lysis buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40, protease inhibitor cocktail (Sigma)) for 10 min on ice ...
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bioRxiv - Bioengineering 2024Quote: The fibrin substrate was made using a concentration of 8 mg of fibrinogen from bovine plasma (Sigma Aldrich, F8630) per mL of GM+ to 0.4 U of thrombin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Pancreas islets were isolated from mouse (8 weeks male C57BL/6J) pancreas by collagenase (1 mg/ml, Sigma-Aldrich) injection in the bile duct and handpicked under a binocular microscope (Leica) ...
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bioRxiv - Microbiology 2023Quote: ... and used to transduce TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The collected clear fluid was transferred into a 1.5 ml reaction tube filled with 8 M urea (Sigma Aldrich). All samples were stored at -20°C until mass spectrometry analysis.
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bioRxiv - Molecular Biology 2023Quote: ... we extracted RNA from 30 individuals fed for 8 days with 5% Sucrose and 0.1% Nipagin in the control group and additionally 2.5nM Paraquat (Sigma 856177) in the treated group ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was mixed with an equal volume of 0.2 M sodium citrate (pH 5.5) and incubated at 37°C for 8 h with or without (as a negative control) trehalase (Sigma). The amount of glucose generated from trehalose was assayed using a Glucose Assay Kit (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The peak fractions were pooled and concentrated to 8-9 mg ml-1 using a 100-kDa concentrator (Millipore) for cryo-EM studies.
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bioRxiv - Cancer Biology 2022Quote: ... The total protein was then resolved on SDS-PAGE (8-12%) and transferred onto PVDF membrane (Millipore, Massachusetts, USA). After the transfer ...
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bioRxiv - Biochemistry 2023Quote: ... Fragmented gDNA or RNase H-digested DNA (4 μg) were immunoprecipitated with 8 µg of S9.6 antibody (Millipore #MABE1095) overnight at 4°C in DRIP binding buffer (10 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then resuspended in transduction medium consisting of ENR medium supplemented with 8 µg/ml polybrene (Sigma-Aldrich), 10 mM of nicotinamide (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... Livers of zebrafish larvae with a SL smaller than 8 mm were directly mounted in Omnipaque350 (Sigma-Aldrich, Histodenz) for imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... and cells were infected via centrifugation at 150 xg for 1 hr in 8 µg/mL polybrene (EMD Millipore). After 3 days ...
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bioRxiv - Physiology 2023Quote: ... Male mice (8-9 wks) were treated with a single dose of tamoxifen (40 mg/kg i.p.; Sigma-Aldrich) to induce recombination or corn-oil vehicle as a control at -4 days relative to mini-pump implantation (see below) ...
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bioRxiv - Neuroscience 2023Quote: ... The input sample and washed beads sample were subjected to 8% SDS-PAGE gels and transferred to 0.45 μm PVDF membranes (Millipore), the membrane was blocked by 5% Nonfat dry milk (BIO-RAD ...
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bioRxiv - Developmental Biology 2023Quote: ... filtered through 0.45 μm sterilization filters and added to HC11 cells together with 8 μg/mL polybrene (Sigma-Aldrich). Transduction efficiency was monitored by checking the expression of green fluorescent protein (GFP) ...