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Citations for Millipore Sigma :
2951 - 3000 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally washed 3 times in PBS-Tween 20 0.1% and mounted in Mowiol 4-88 (81381, Sigma).
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bioRxiv - Systems Biology 2022Quote: ... Formic acid-extracts were spun down at 13k rpm for 10 minutes and a total of 2 μl supernatant was spotted 0.5 μl at a time near the base of a PEI-Cellulose F thin layer chromatography plate (Millipore). The spots were dried and nucleotides were resolved using freshly prepared 1.5M KH2PO4 (pH 3.4) ...
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bioRxiv - Microbiology 2022Quote: ... Nexcelom Biosciences) at the time of harvest by staining with a 0.2% final concentration of trypan blue (Sigma; T8154) in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... Thereafter the sections were rinsed three times in 0.1 M sodium cacodylate buffer (pH 7.2-7.4) (Sigma-Aldrich, Germany) and incubated with 1 % osmium tetroxide (Science Services ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times for 5 min with PBS and permeabilized for 1h at room temperature (permeabilization buffer: 2,5% donkey serum (Sigma), 1% BSA ...
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bioRxiv - Immunology 2020Quote: ... the sections were washed 3 times in PBS and permeabilized in PBS containing 0.3% Triton-X100 (#X100, Sigma-Aldrich) for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... with the exception that the samples were spiked with isotope-labelled peptides and MSRT retention time peptides standards (Sigma), prior to MS analysis.
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed three times in PBS and then fixed in 4% formaldehyde (paraformaldehyde powder, 95%, 158127-2.5KG, Sigma) at room temperature for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... Cover slides were washed three times with 0.1 M sodium cacodylate (pH 7.2) (>98% purity, Sigma Aldrich, Steinheim, Germany) and post-fixed for 1 h with 1% osmium tetroxyde in the same cacodylate buffer ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed three times in PBS with 0.05% Tween 20 (PBS-T) and then blocked with 1x casein (Sigma) for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... were collected from the top of the gradient and the fraction material was cleaned by washing three times and resuspended in 0.02 µm-filtered FSW using 100 kDa Amicon-ultra filters (Merck Millipore). Vesicles were detected in fractions with densities of 1.05–1.07 g ml−1 (fractions 3-5 from the top).
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bioRxiv - Genetics 2022Quote: ... cells were washed three times in YPD (30°C) containing 0.1 mg/ml Pronase E (Sigma-Aldrich; Catalogue # P5147), resuspended in SC minimal medium containing nocodazole (15μg/ml) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were washed five times with 100 µl/well DPBST and 50 µl/well 3,3′,5,5′-tetramethylbenzidine (TMB) substrate solution (Sigma; T0440) was added to each plate ...
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bioRxiv - Genetics 2022Quote: ... Slides were then washed three times with Tween20 in 1xPBS (0.02% final, Sigma; room temperature, 5 minutes each wash), and blocked with BSA solution (2% BSA ...
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bioRxiv - Cell Biology 2022Quote: Cells at 80–90% confluence were washed two times with minimal media [Glasgow minimal essential media (Sigma-Aldrich G6148) buffered with 10 mM HEPES] at 4 °C and then incubated with toxin (anthrax toxin ...
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bioRxiv - Developmental Biology 2022Quote: ... and spun down three times before being resuspended to a concentration of 150,000 cells/mL in 20% Optiprep (Sigma) for inDrop sorting.
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bioRxiv - Biochemistry 2022Quote: ... 10 g/L bovine serum fraction V protein and 1.5 mM β-mercaptoethanol) and disrupted by shaking five times for 1 min with glass beads (0.45–0.50 mm diameter, Sigma-Aldrich). The crude extracts were recovered by washing the glass beads four times with 5 ml TES ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed six times with PBS and incubated for 1 h with the secondary antibodies (phalloidin (Sigma - P1951), 1:500 ...
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bioRxiv - Genetics 2020Quote: ... Beads were then washed three times with lysis buffer and eluted in lysis buffer supplemented with 0.5mg/mL 3xFLAG peptide (Sigma). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Bioengineering 2019Quote: ... The filtrate was then concentrated ∼60 times using 15mL 10kDa weight cut-off Amicon Ultra Centrifugal Filters (EMD Millipore) at 3,901xg ...
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bioRxiv - Systems Biology 2019Quote: ... Beads were washed 4 times with 1 mL cold TBS pH 7.4 + 0.05% Igepal CA-630 (Sigma Aldrich Corporation). Protein was eluted with 5 units AcTEV™ Protease (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... three times for 10 minutes and then incubated with primary antibody (1:250 Rabbit anti-CGRP, Millipore-Sigma, PC205L) in blocking buffer solution (1x PBS ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... three times for 10 minutes and then incubated with primary antibody (1:250 Rabbit anti-CGRP, Millipore-Sigma, PC205L) in blocking buffer solution (1x PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then washed 3 times with TBS containing 1% BSA and 0.1% w/v sodium azide (Sigma Aldrich). After staining ...
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bioRxiv - Neuroscience 2019Quote: ... Sections from VGLUT2-EYFP and VGLUT2-ChR2 mice were processed In parallel and for the same period of time (15 min) in 3.3’-diaminobenzidine tetrahydrochloride (DAB, Sigma fast tablets; Sigma), rinsed in KPBS ...
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bioRxiv - Cell Biology 2020Quote: ... the beads were washed with 1% Trition buffer three times and then incubated in 30 μl of 1% Trition buffer with 0.2 μg/μl of 3xFLAG peptide (Sigma) at room temperature for 20 min ...
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bioRxiv - Systems Biology 2021Quote: ... The retention times of the eluted peaks were compared to those of commercial analytical standards (Sigma-Aldrich, Madrid, Spain). Metabolite concentrations were quantified by the calibration graphs (R2 value > 0.99 ...
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bioRxiv - Genomics 2020Quote: ... 2 mM DTT) by performing buffer exchange three times using one Amicon 30 kDa MWCO spin column (Millipore UFC903008). The purified protein was then transferred to a new tube ...
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bioRxiv - Molecular Biology 2021Quote: ... A 5 µL aliquot for each time point was removed and quenched with 5 µL nuclease P1 (Sigma #N8630) in a 100 mM sodium citrate suspension (pH 4.7 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed four times with cell staining medium (CSM, PBS with 0.3% saponin, 0.5% bovine serum albumin (BSA, Sigma Aldrich) supplemented with 2 mM EDTA (Stemcell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... HFF monolayers were infected at an MOI of 0.5 for 3.5h then washed four times with PBS before adding switch media (RPMI (Sigma, R4130) with 1% FBS ...
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bioRxiv - Microbiology 2020Quote: ... Ten times serial dilutions of this culture were then plated on LB agar plates supplemented with ciprofloxacin 1μg/mL (Sigma) and the plates incubated overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the conjugated antibody was washed several times with W-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 50kDa cut-off (Millipore-Sigma), and quantified using a NanoDrop spectrophotometer (280 nm) ...
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bioRxiv - Pathology 2021Quote: ... Japan) were immunized (four times at 2-week intervals) with the purified protein conjugated to TiterMax Gold (Sigma-Aldrich). Rabbits were sacrificed under excess anesthesia with pentobarbital sodium (64.8 mg/kg) ...
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bioRxiv - Biochemistry 2021Quote: ... Culture was washed three times in PBS (300 g, 5 minutes) and parasites isolated from erythrocytes using 0.03% (w/v) saponin (Sigma) on PBS containing protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... washed three times with 0.1% sodium cacodylate buffer and once with cacodylate buffer containing 1mg/ml 3,3’-DAB (Sigma-Aldrich). Pellets were then incubated 20 minutes in 1mg/ml DAB (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were washed 3 times with 0.1 M phosphate buffer and incubated with filtered thiocarbohydrazide (TCH, Sigma, 223220) for 45 mins at 40 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed in PBST 3 times for 10 minutes and mounted using MOWIOL-DABCO Mounting Media (Sigma, #10891) and then dried at room temperature overnight in a dark box ...
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bioRxiv - Immunology 2021Quote: ... Slides were washed 5 times with PBS and blocked with blocking buffer (PBS + 1% bovine serum albumin (BSA, Sigma, cat no ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times for 5 min and incubated with anti-mouse-HRP antibody (0.2 μg/ml – Millipore) for 1 h in TBST ...
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bioRxiv - Systems Biology 2019Quote: ... We then washed the cells once in PBS and three time in PBS with 0.1% Tween 20 (Sigma-Aldrich; PBS-T for 5 min each and incubated with 30 microliter of a 1:1000 dilution of Alexa Fluor 647 conjugated goat anti-rabbit or goat anti-mouse secondary antibody in Odyssey blocking buffer for 60 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were rinsed four times with cacodylate buffer and post-fixed with 0.8% K3Fe(CN)6 (Sigma-Aldrich) and 1% OsO4 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then washed with PBS-T four times for 5 minutes each and treated with 0.1% Sudan Black-B solution (Sigma) for 10 minutes to quench autofluorescence ...
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bioRxiv - Cell Biology 2021Quote: Tam-treated MBNL1Fl/Fl and MBNL1Fl/Fl-Tcf21iCre cardiac fibroblasts were infected with either AdGFP (controls) or AdMBNL1 for 36 hours at which time transcription was inhibited with 2μg/ml Actinomycin-D (Sigma). RNA was isolated from each group after 0 ...
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bioRxiv - Genomics 2021Quote: ... rotating for 10 min at room temperature each time and then blocked in Pax-DG (10 g BSA (Sigma), 3g Deoxycholate Acid (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... The strain was cultured for 6 hours in BHI with 0.2% Tween 80 and washed 3 times with M9 minimal medium (11.28 g/L M9 salts (Sigma-Aldrich), 0.1 mM CaCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... The wells were again washed three times with TBST before incubated with streptavidin-conjugated with horseradish peroxidase (HRP) (1:5,000, Cat S2438, Sigma). After 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was washed four times with TTBS and secondary antibody goat-anti rabbit-HRP (catalog no. A7058, Sigma) was added at 1:60,000 in 5% milk for 2 hours at RT ...
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bioRxiv - Cell Biology 2020Quote: ... For each time point the cells were rinsed in cold PBS before adding 40 μl RIPA buffer (EMD Millipore) with protease inhibitor mixture (Roche) ...