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Citations for Millipore Sigma :
3001 - 3050 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Cells were first washed two times on ice with 10 ml of PBS containing 100 μg/mL cycloheximide (Sigma). Cells were lysed in 50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and after a final two times PBS wash embedded using an antifade solution [10% (v/v) mowiol (Sigma, #81381), 26% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... Proliferation at corresponding time points was assessed by MTT (Methylthiazolyldiphenyl-tetrazolium bromide) assay according to the manufacturer’s recommendations (Sigma). For each time point ...
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bioRxiv - Developmental Biology 2021Quote: ... at which time 15 larvae were transferred to freshly prepared 1% (w/v) DSS (SIGMA, D8906-10G, MW>500,000) in E3 for a single overnight treatment ...
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bioRxiv - Microbiology 2020Quote: ... The beads were washed 4 times with the wash buffer and loaded onto 0.5 ml Ultrafree-MC centrifugal filters (Millipore). The immobilized material was eluted with the wash buffer containing 0.3 M imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed three times with lysis buffer-PBS at 1:4 and eluted with 3xFLAG peptide (Sigma-Aldrich) or Laemmli sample buffer ...
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bioRxiv - Immunology 2021Quote: ... the cells were washed three times with warm PBS and incubated with fluorescein diacetate (8 μg/mL, Sigma-Aldrich) and propidium iodide (20 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed three times with PBS and permeabilized with 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO) for 10 minutes at RT ...
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bioRxiv - Immunology 2020Quote: ... Plated were washed three times with PBST (PBS+0.05% Tween-20) and blocked with 2% BSA (Sigma-Aldrich, #A8022) in PBST for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed five times with PBS and incubated in ExtrAvidin-Alkaline Phosphatase (Sigma, St. Louis, MO) diluted 1:1000 in PBS and incubated for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... the oocytes were washed three times and incubated with FITC-labelled mouse anti-α-tubulin (1:200, Sigma-Aldrich) for 1 h at 37°C.
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bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5 % fish skin gelatin (Sigma Aldrich) for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed three times in PBS and lysed in RIPA supplemented with 1x protease inhibitor cocktail (Millipore), 100 mM Na3VO4 and 500 mM NaF ...
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bioRxiv - Immunology 2022Quote: ... samples were re-loaded four times before washing with 1 mL of 2% acetonitrile (ACN) (#1000292500, Sigma-Aldrich, Merck) in 0.2% acetic acid ...
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bioRxiv - Biochemistry 2022Quote: ... The incubated beads were washed 3 times with HAC buffer followed by eluted in 1 M imidazole (Sigma, #IX0005) in HAC buffer.
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bioRxiv - Bioengineering 2022Quote: ... sections were rinsed with PBS 3 times and then mounted on microscope glass slides with Mowiol (Sigma-Aldrich, 81381). The expression of proteins was visualized with a Leica TCS SP5X microscope and images were processed with ImageJ (version 1.53f51) ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse imaging was performed in YES media in a CellASIC ONIX microfluidics perfusion system (Millipore Sigma, Burlington, MA). Cells were loaded into Y04C plates for 5 s at 8 psi ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated at the time of switching to DM with 10 µM of GW4869 (Sigma-Aldrich, Gillingham, UK), an inhibitor of neutral sphingomyelinase 2 (nSMase2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Beads were then washed three times with lysis buffer and eluted in lysis buffer supplemented with 0.5mg/mL 3xFLAG peptide (Sigma). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondrial OCR was measured in a time course before and after the injection of 1.5 μM Oligomycin (Millipore, 495455), 2.5 μM FCCP (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed three more times and incubated with 1 µg/ml of DAPI (Sigma, catalog # D9542-10MG) for 10 minutes before being washed with PBS ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then washed with TBST three times for 10min before incubating with a second antibody (anti-rabbit (Sigma), 1:2000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... The fixed plates were washed with PBS (200 µL per well) three times and then permeabilized and blocked using 0.1% Triton X-100 (Sigma) with 10% donkey serum (v/v ...
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bioRxiv - Microbiology 2022Quote: ... and the cells were washed three times in PBS and incubated with 150 μg/ml of gentamicin (Sigma-Aldrich) and 50 μg/ml penicillin G (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Permeabilized cells were subsequently washed three times with PBS and blocked with PBS+ (5 g/L BSA (Sigma Aldrich) and 1.5 g/L glycine (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with DPBS for 5 min and blocked in 1% BSA (Sigma-Aldrich, cat# A4503) in PBS-T (DPBS with 0.05% Tween 20 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was washed three times in PBST and then developed by using Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Genetics 2024Quote: ... Zygotes with visible pronuclei washed 3 times in HTF medium and transferred to KSOM medium (Millipore, MR-106-D) and cultured in low oxygen chamber filled with the mixture gas (5% O2/ 5% CO2) ...
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bioRxiv - Microbiology 2023Quote: ... The coverslip was rinsed 3 times with PBS-T and incubated with Alexa Flour conjugated secondary antibodies (Sigma-Aldrich) and Hoechst 33342 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... anti-FLAG magnetic beads were washed 5 times with cold wash buffer (GTEN buffer plus 0.1 % NP-40 (Sigma) and Roche protease inhibitor (50 ml/ 1 tablet)) ...
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bioRxiv - Microbiology 2024Quote: ... Secondary-probed blots were washed three times with TTBS followed by TBS and incubated with Immobilon chemiluminescent substrate (Millipore). Images were acquired using a ChemiDoc Imager (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were washed three times and the revelation was made in DAB solution containing 3,3’-Diaminobenzidine (DAB) and urea in H2O (Sigma). The reaction was stopped in PBST ...
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bioRxiv - Immunology 2024Quote: ... Excess bacteria was then washed off and cells were incubated for the remaining time with 50µg/mL gentamycin (Sigma).
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bioRxiv - Microbiology 2024Quote: ... The antigen-bound magnetic beads were washed five times with lysis buffer and eluted with 2.5 volumes of 3× FLAG peptide (150 ng/μl, Sigma) for 3 h at 4°C with gently shaking ...
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bioRxiv - Immunology 2024Quote: ... The homogenate was mixed 50 times using two syringes connected via a 25G needle and passed 5 times through a polyethersulfone membrane filter with pore size 0.22 μm (Millipore) and the filter-sterilized adjuvant was stored at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Cryosections were washed three times 5 min with PBS and were mounted with Fluoroshield with DAPI (Sigma Aldrich, F6057). Images were acquired using an Axio Imager M2 with Apotome 2.0 and 20x objective (Zeiss ...
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bioRxiv - Bioengineering 2023Quote: ... plates were washed three times in PBS with 0.05% Tween 20 (PBS-T) and then blocked with 1x casein (Sigma) for 2 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... were pre-prepared by washing 3 times in immunoprecipitation buffer then resuspended in 200μl immunoprecipitation buffer and coated with 2 μl FLAG M2 (Merck Millipore) or IgG (Merck Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... washed three times in sterilised water and sown on half-strength Murashige and Skoog media (1/2 MS) (Sigma), 3 mM MES-KOH (pH 5.7) ...
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bioRxiv - Molecular Biology 2023Quote: ... then fixed with 4% paraformaldehyde for 30 min at room temperature and washed 3 times in PBS.Cells were permeabilized with 0.1% Triton-X100 (Sigma T8787) and washed 3 times in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... the blot was washed for for three more times before adding a Immobilon® Western Chemiluminescent HRP Substrate (Millipore) to the blot ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed three time in PBS and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Coverslips were mounted onto glass slides in Mowiol-DABCO ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed three times in PBS-T buffer and incubated with HRP-conjugated anti-rabbit antibody (1:10,000, Sigma) for 4 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells were finally washed three times and incubated in 4,6-diamidino-2-phenylindole (DAPI) (1 µg/ml; Sigma-Aldrich) for 5 min in dark ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were washed 3 times with PBS-T (0.2% Triton X-100 in PBS (PBST) with 0.01% sodium azide (Sigma Aldrich)) and blocked for 1 hour in 10 % Goat Serum (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Seeds were surfaced sterilized with 70% v/v ethanol followed by 0.3% v/v sodium hypochlorite then rinsed three times with sterile MiliQ water and plated on Murashige and Skoog basal medium (4.44 g L−1, Sigma) 0.8% w/v agar supplemented with 0.05% w/v glucose ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-BirA* TBCCD1 and Flag-BirA* cell lines were incubated for different times in complete media with (a) 0.5 μg/mL or 1 μg/mL tetracycline (SIGMA) and 50 μM biotin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... the grid was washed three times with 15 μL ddH2O and negatively stained with 2% OsO4 (Sigma Aldrich, USA) and 2% uranyl acetate (Sigma Aldrich ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Each well was washed three times and extra-cellular fluorescence quenched by adding 0.2 uM syringe-filtrated trypan blue (Sigma). Cell viability was determined using 7-AAD dye (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... and cells were washed four times with warm PBS before incubation with 1 μM potassium iodide (KI, Sigma-Aldrich) in PBS for 1 hour at 37°C ...