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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Fixed cells were blocked and permeabilized overnight at 4°C in block solution (1x PBS + 5% bovine serum albumin (Sigma-Aldrich #A3803) + 0.5% Triton-X-100) ...
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bioRxiv - Cancer Biology 2023Quote: ... were recovered from Matrigel using 3 mM EDTA in DPBS, washed, centrifuged (200g, 5 min, 4°C) and lysed with 1x RIPA buffer supplemented with PPI (1:100, Sigma- Aldrich, 04693132001), and benzonase (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... to an optical density at 600 nm (OD600) of 0.5 before 5-mL aliquots were treated with 180 uM 4-DPS (Sigma; Cat# 143057-1G) for 20 min at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were then rinsed 3 times with PBS and mounted onto microscope slides with 5 μl Mowiol 4-88 (Sigma-Aldrich #81381). Cells were visualized and imaged with a Nikon Ti-Eclipse A1M Confocal Microscope using a 60X objective ...
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bioRxiv - Neuroscience 2024Quote: ... 4-5 non-embedded organoids at day 30 of organoid culture were pooled per sample and dissociated using Accuatse (Sigma, cat.no. A6964). Samples were first incubated with Accutase for one hour at 370C under dynamic conditions ...
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bioRxiv - Biochemistry 2024Quote: ... blotted and incubated overnight in 5% skim milk (GERBU Biotechnik, 70166) in TBST at 4°C with an anti-RAS antibody (Millipore, 05-516). As a loading control ...
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bioRxiv - Developmental Biology 2024Quote: ... differentiation day 18 control and proximal-biased organoids were incubated at 37°C/5% CO2 for 4 hours in TeSR-E6 supplemented with 10 µg/mL TRITC-albumin (Sigma Aldrich, A2289). Control organoids were incubated with TeSR-E6 alone ...
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bioRxiv - Physiology 2019Quote: ... transgenic soybean hairy roots and tobacco leaves were used to determine GUS enzyme activity by measuring the fluorescence of 4-methylumbelliferone (4-MU) produced by GUS cleavage of 4-methylumbelliferyl-β-d-glucuronide (4-MUG, Sigma, USA) according to the published procedure as described previously (Jefferson ...
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bioRxiv - Molecular Biology 2021Quote: ... male WT (GR fl/fl, Cre/−) and GR knockout (GR fl/fl, Cre/+) mice (N=6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
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bioRxiv - Molecular Biology 2021Quote: ... heterozygote (HNF4α fl/+, Cre/+), and knockout (HNF4α fl/fl, Cre/+) mice (N=3-6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
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bioRxiv - Plant Biology 2023Quote: CFDA: A 100 μl drop of 5(6)-carboxyfluorescein diacetate (CFDA, optimum excitation 490 nm, emission 515 nm) (C8166, Sigma, stock solution 50 mg/ml in acetone) was applied to the cut edge of a single cotyledon at a concentration of 0.1 mg/ml in distilled water ...
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bioRxiv - Neuroscience 2022Quote: ... and fixed with 4% PFA / 4% sucrose (Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used 4-Nitroquinoline 1-oxide (4-NQO) (Sigma–Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 mM ethylenediaminetraacetic acid (60-00-4, Sigma-Aldrich), 0.1 M tris-HCl (1185-53-1 ...
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bioRxiv - Neuroscience 2021Quote: ... 100-300 μM 4-aminopyridine (4-AP, Sigma-Aldrich) and 10-30 μM XE-991 dihydrochloride (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... α-cyano-4-hydroxycinnamate (4-CIN, 250 µM, Sigma-Aldrich); iodoacetic acid (IAA ...
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bioRxiv - Neuroscience 2020Quote: ... then 4 % PFA (paraformaldehyde, Sigma Aldrich, 30525-89-4) in 0.1 M PB (phosphate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 4-hydroxytamoxifen (4-HT; Sigma # H7904, St. Louis, MO) was injected into 2-3 months old PDGFRα-CreERTM;ROSA26-iDTa mice ...
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bioRxiv - Developmental Biology 2022Quote: ... 4-Hydroxytamoxifen (4-OHT, Sigma-Aldrich, cat. no. H7904) was added on day 5 at a concentration of 0.3 µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... 4-hydroxytamoxifen (4-OHT; H6278; Millipore Sigma, MA, USA) was made as described previously [19] ...
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bioRxiv - Neuroscience 2024Quote: ... and 4-aminopyridine (4-AP, 100 μM, Sigma Aldrich) were used ...
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bioRxiv - Molecular Biology 2024Quote: ... 4-nitroquinoline 1-oxide (4-NQO) (Sigma-Aldrich #N8141) was added to a concentration of 1 μg/mL or 5 μg/mL ...
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bioRxiv - Genetics 2021Quote: ... was propagated and maintained by transferring shoot segments of 3-4 cm with 1-2 young leaves to fresh one-half Murashige and Skoog (Beijing, China, Sigma-Aldrich). Plantlets were grown at 23 ℃ under a 16 h light/8 h dark cycle with a light intensity of illumination of 5000 lux provided by cool white fluorescent lamp tubes ...
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bioRxiv - Microbiology 2020Quote: ... without L-glutamine and without phenol red (Biochrom) supplemented with 30 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma-Aldrich) with a pH of 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... the slides were then incubated overnight at 4°C with rabbit polyclonal to elafin in 2% BSA at 1:100 dilution (Sigma, HPA017737). The signal was detected using the rabbit horseradish peroxidase EnVision kit (DAKOCytomation ...
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bioRxiv - Neuroscience 2019Quote: ... were expressed in 3T3 cells and IPed using 1µg anti-Fyn (mAb, sc-434, SantaCruz) or 4 µg anti-MAP2 (HM-2, mAb, Sigma, Cat # M4403) and Protein G beads (CalBiochem) ...
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bioRxiv - Immunology 2021Quote: ... mice were anaesthetised using inhaled isoflurane (4% (v/v) isoflurane and 2 l O2/min) and 30 μl of LPS (250 μg/mL, Sigma-Aldrich) or vehicle PBS were administered intranasally ...
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bioRxiv - Biochemistry 2021Quote: ... at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich), 0.1% formic acid) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... Samco Trident Vial Tall with PP Screw Cap) were incubated for 4 hours with 2 % aqueous Tween solution (Tween20, Sigma-Aldrich), then rinsed with deion ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and viral envelope (pMD2G) constructs at a 4:2:1 ratio with 1 mg/mL polyethylenimine (PEI, linear MD 25,000 Da, Sigma, Cat# 408727). The ratio of total transfected DNA to PEI was 1:3 (1 µg DNA:3 µg PEI) ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 cells were lysed with RIPA buffer for 2 h at 4°C and tissues were disrupted and lysed in RIPA buffer (Sigma-Aldrich) using a MagNA Lyser instrument (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were surface sterilized and kept for stratification in dark at 4 °C for 2-3 days on half strength MS-agar (Millipore Sigma) plates ...
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bioRxiv - Biophysics 2020Quote: ... maintains a pluripotent state of the stem cells.2–4 Cells were cultured in Glasgow modified Eagle’s medium (GMEM, Sigma Aldrich, Germany) supplied with 5.5 ml non-essential amino acids (Gibco Science ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... Dimethyl LV labeling for geminal pair determination used 150 mg/L 2-Keto-(3-methyl-13C)-butyric-4-13C,3-d acid sodium salt (Sigma 589063). Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887 ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Bioengineering 2022Quote: ... A 2 μL aliquot of 100 μM aptamer in PBS was thawed and mixed with 4 μL of 10 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich) for 8 hours to reduce the disulfide bond ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:200 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... Embryos at 2 dpf (days post-fertilization) were removed from their chorions and anesthetized with 4% tricaine (MS-222, Sigma Aldrich). The embryos were placed in a 1% agarose-coated dish with small holes ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were fixed with 4% formaldehyde for 20 min and stained with 1x crystal violet solution (2% crystal violet (Sigma-Aldrich), 20% ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads, Sigma-Aldrich) previously washed in Rac buffer (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Afterwards the medium was switched and cells were cultivated for 4 days in DMEM containing 10% FCS and 2 μM insulin (Sigma Aldrich) to induce adipogenic differentiation ...
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bioRxiv - Cancer Biology 2020Quote: Non-synchronized and synchronized cells were fixed using 70% Ethanol for 48 hours at 4°C then washed with PBS+2% goat serum (Sigma, UK) and resuspended in 1mL of ice-cold PBS ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl and 0.1% Tween-20 or TBST buffer, 2 h) and incubated (4°C, overnight) with biotinylated BSI-B4 lectin from Bandeiraea (Griffonia) simplicifolia (Sigma-Aldrich, #L3759-1MG ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...