Labshake search
Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Exponentially growing RPE-1 (human retinal pigment epithelial) cells were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
-
bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
-
bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MN, USA) in Tris buffered saline (TBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... washed three times in PBS/2%BSA for 5 min and then permeabilized using 0.1% Triton X-100 (Sigma-Aldrich) for 20 min followed by another washing cycle ...
-
bioRxiv - Neuroscience 2023Quote: Cell proliferation was assessed at DIV2 by co-incubating cells with 10μM of 5-bromo-2’-deoxyuridine (BrdU) (#B5002, Sigma-Aldrich) in the last 4h of the pharmacological treatments ...
-
bioRxiv - Neuroscience 2023Quote: ... After blocking tissue with 5% swine serum and 2% BSA in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
-
bioRxiv - Physiology 2023Quote: ... were transferred to OP50-seeded plates or RNAi-seeded NGM plates supplemented with 50 μM of 5-fluoro-2-deoxyuridine (FUDR, Sigma) to prevent progeny growth ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were washed four to five times with warm media (DME-HG for MEFs and DME-HG/F12 for MCF10A) and pulse-labeled with 100 µM 5- chloro-2’-deoxyuridine (CldU; Sigma) for 20 min at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 mL of a 2.0×10^5 cells/mL solution were added to single cell culture dishes (EP0030700112, Sigma-Aldrich) and incubated overnight ...
-
bioRxiv - Neuroscience 2023Quote: 5 dpf WT and scn1lab larvae were incubated in acridine orange (AO) (10 μM, Sigma Aldrich, CAS: 65-61-2) solution for 20 minutes followed by three 5 minute washes in embryo media ...
-
bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...
-
bioRxiv - Genetics 2022Quote: Fertilised eggs were identified by the presence of a sperm entry point and placed in wells cut into 2% noble agar lined 50 mm petri dishes containing 5% Ficoll 4000 (Sigma) in 1xMMR ...
-
bioRxiv - Biophysics 2023Quote: ... The samples were blocked and simultaneously permeabilized using 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma Aldrich), in PBS (room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... Then the brains were dissected to 2 mm slices and incubated for 10 minutes in freshly prepared 0.5% TTC solution (2, 3, 5-Triphenyltetrazolium chloride, Sigma-Aldrich). Afterward ...
-
bioRxiv - Cancer Biology 2023Quote: ... two to four-month-old mice were gavaged 3 times over 5 days with 2 mg tamoxifen (TX; Sigma #T5648) in corn oil (Sigma #C8267) ...
-
bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane (5% induction, 1-1.5% maintenance) and administered a subcutaneous dose of chlorprothixene (2 mg/kg, Sigma-Aldrich). During the mapping session ...
-
bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
-
bioRxiv - Cancer Biology 2023Quote: ... permeabilized in 0.3% PBS-Triton X-100 for 40 minutes and blocked in PBS/0.1% Triton X-100 containing 2% BSA and 5% donkey serum (#D9663, Sigma-Aldrich). Primary antibodies were incubated overnight at 4°C in the blocking buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
-
bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally three times daily for five consecutive days with 5-bromo-2’-deoxyuridine (BrdU, Sigma B9285-1G) diluted to 30 mg/kg in PBS ...
-
bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
-
bioRxiv - Cell Biology 2024Quote: Propidium staining was conducted by 2 min incubation of 5-day-old Arabidopsis seedlings in propidium iodide (Sigma Aldrich P4864) diluted to 1 µg/mL in water ...
-
bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid, ABMBA, #R435902, Sigma) and centrifuge-filtered (0.22 µm hydrophilic PVDF membrane ...
-
bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO], 20 ng/ml bFGF [Sigma], and 20 ng/ml EGF [Sigma]) before collection for single-cell analysis ...
-
bioRxiv - Plant Biology 2021Quote: The dried samples were derivatized for 2 hours at 37 °C in 50 μI of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
-
bioRxiv - Immunology 2024Quote: ... we used inhibitors of ERK1/2 (Trametinib, SelleckChem, Catalog # S2673), p38 (Losmapimod, Selleckchem Catalog #S7215), NF-kB (BAY11-7082, Catalog # S2913) and JNK inhibitor (Millipore-Sigma Catalog # CAS 129-56-6) 2 hr before stimulation with R848 or Poly I:C until sample collection at 8 or 24hrs for mRNA or protein levels of antiviral and inflammatory cytokines and chemokines.
-
bioRxiv - Cancer Biology 2021Quote: 5-Fluoro-5’-Deoxyuridine (Sigma Aldrich, F8791) and Oxaliplatin (Sigma Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... 5 ml of HNO3 5% (Sigma, 84378) were added to each sample and they were placed 1h at 95°C ...
-
bioRxiv - Cell Biology 2022Quote: ... and 5-PVA (5-phenylvaleric acid, Sigma) were dissolved in equimolar amount of NaOH to prepare a 0.5M stock solution with pH 7.
-
bioRxiv - Microbiology 2021Quote: ... 5-aza-cytidine (5-AZAC, Sigma-Aldrich) and 5-deoxy-azacytidine (DAC-5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 5-fluorouacil (5-FU; Sigma-Aldrich) were dissolved in DMSO ...
-
bioRxiv - Physiology 2024Quote: ... 5-hydroxytryptamine (5-HT, serotonin) (Sigma-Aldrich) and histamine (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were cultured in a 24-well plate (500’000 cells/well) in complete IMDM supplemented with 6 ng/ml IL-6 (Sigma), 6 ng/ml IL-3 (eBioscience ...
-
bioRxiv - Molecular Biology 2019Quote: ... Supernatants were incubated with 6 µl anti-HA (12CA5, in-house preparation) or 6 µl anti-T7 (69522, Millipore) and protein G-dynabeads (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... Loaded PDMS chambers were incubated for 30 minutes at 37°C and after polymerization muscle bundles were kept in normal proliferation medium containing 1.5 mg/ml 6-aminocaproic acid (6-ACA) (Sigma). After 2 days ...
-
bioRxiv - Neuroscience 2021Quote: ... Adult zebrafish (6 female and 6 male) were anesthetized in 0.02% tricaine (E10521, Ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich). Shallow lesions were made on the skin (10 on each side ...
-
bioRxiv - Neuroscience 2022Quote: Partial bilateral neostriatal dopamine lesions were made by injecting 6-OHDA (6 μg/μL, Sigma-Aldrich, St. Louis, MO) dissolved in ascorbic saline (de-ionized water containing 0.9% NaCl and 0.1% ascorbic acid ...
-
bioRxiv - Cell Biology 2022Quote: C57Bl/6 mice (6 weeks-old) were treated with an ip injection of 1500 mg TAA/kg (Sigma-Aldrich) to induce ALF ...
-
bioRxiv - Physiology 2022Quote: ... 0.5M K4[Fe(CN)6] and 0.5M K3[Fe(CN)6] with 1 mg/ml X-Gal diluted in DMF (all Sigma)) ...
-
bioRxiv - Neuroscience 2024Quote: ... each mouse received a bilateral injection of 1.25 μl of 6-hydroxydopamine hydrochloride (6-OHDA, Sigma-Aldrich, 4μg/μl) or vehicle (0.9 % NaCl + Ascorbic Acid 0.02% ...
-
bioRxiv - Immunology 2024Quote: ... BEAS-2B.MR1-GFP cells were incubated with 94 μM 6-formylpterin (6-FP, Schircks) or the equivalent volume of 0.01 M NaOH (Sigma) overnight at 37°C at 5e4/well in a 24-well plate ...
-
bioRxiv - Neuroscience 2023Quote: ... 6-OHDA rats received 2.3 μL bilateral injections of 6-OHDA hydrobromide (6 μg combined with ascorbic acid dissolved in 2.3 μL sterile 0.9% NaCl, Sigma Aldrich) in the SNc in each cerebral hemisphere ...
-
bioRxiv - Developmental Biology 2023Quote: Pups were injected once daily intraperitoneally with 6-hydroxydopamine hydrochloride (6-OHDA) 50ug/g of body weight (Sigma H4381) from post-natal day 0 to 5 ...
-
bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
-
bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
-
bioRxiv - Genetics 2021Quote: The Extensor Digitorum Longus (EDL) muscle was injured by intramuscular injection of 50 µL of 5 µM cardiotoxin (CTX) (Sigma, 11061-96-4). Mice were treated with carprofen 20 minutes prior to CTX injection and were injected with CTX under anesthesia by isoflurane ...
-
bioRxiv - Plant Biology 2019Quote: ... raised against gel-purified ATLP1 using alkaline phosphatase(AP)-conjugated goat anti-rabbit IgG (1:10000 dilution) as the secondary antibody and 5-bromo,4-chloro,3-indolylphosphate(BCIP)/nitroblue tetrazolium (NBT)(Sigma Chemical Co. MS) as the substrates (Safadi et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indoxyl phosphate (BCIP; Sigma Aldrich, Taufkirchen, Germany) for 5-30 min.