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Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the retrovirus supernatant was concentrated by centrifugation at 5000g at 4 °C for 2 h with Amicon Ultra-15 Centrifugal Filter Units (Millipore, Billerica, MA). A human T cell line (Jurkat ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... For immunostaining cells were fixed with 4% PFA in cytoskeleton preservation buffer (10mM 2-(N-morpholino) ethanesulfonic acid (MES, Sigma-Aldrich, M3671) pH 6.1 ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was washed once with TBST and incubated with primary antibodies at 4°C for 12 hrs (Phospho-ERK1/2 1:500 (Sigma Aldrich, #E7028); ERK1/2 1:1000 (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: Cultured cells were fixed with 4% paraformaldehyde (PFA) and 2% sucrose and then stained with Nile red (Sigma-Aldrich, St. Louis, MO) and HCS Cell Mask Blue (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Biochemistry 2022Quote: ... sodium hydroxide (1310-73-2) and α-amylase (DFP Treated, Type I-A, saline suspension, 647-015-00-4) were purchased from Sigma-Aldrich Company Ltd. ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated overnight with retronectin solution (32 µg/mL in PBS, WEHI) at 4℃ followed by blocking with 2% bovine serum albumin solution (Sigma-Aldrich, #A1595) in PBS at 37℃ for 30 min prior to coating with viral supernatant ...
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bioRxiv - Biophysics 2023Quote: ... with a voxel-size of 0.636 × 0.636 × 2 μm) are recorded once before and 4 h after actin-depolymerization using 10 μM cytochalasin D (Sigma-Aldrich, St. Louis) using brightfield and second-harmonic generation imaging microscopy ...
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bioRxiv - Genomics 2023Quote: ... 160 μl of 2x lysis buffer (2 % Triton x-100, 100 mM Tris-HCl pH 8.0, 300 mM NaCl, 0.2% sodium deoxycholate (Sigma-Aldrich, 302-95-4), 10 mM sodium butyrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... An amount of 2-4 mg of whole cell extract as incubated with 25 μL ANTI-FLAG® M2 affinity gel (Millipore Sigma) overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Samples were cryoprotected in a 30% sucrose solution (w/v) overnight at 4°C and frozen in 2-methylbutane (Sigma-Aldrich 106056) on dry ice ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were induced with 2-4% isoflurane and injected with 15 µL of CFA (Sigma-Aldrich F5881. Sigma, St. Louis, MO, USA) subcutaneously in the snout ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were induced with 2-4% isoflurane and injected with 15 µL of CFA (Sigma-Aldrich F5881. Sigma, St. Louis, MO, USA) subcutaneously in the snout ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells exposed to glutamine starvation were also treated with a freshly made 2 mM methionine sulfoximine (MSX, Sigma Aldrich 15985-39-4). The TCA precipitated samples were then centrifuged at 4000 rpm for 5 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... counted and 2-4×106 cells were washed twice in ice cold 0.9% saline made with NaCl (Sigma Aldrich, 31434-500G-M) and ultrapure HPLC grade water (Alfa Aesar ...
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bioRxiv - Evolutionary Biology 2019Quote: Five skate embryos were cultured from stage 23–30 in 900 mL of salt water (Instant Ocean) with/without all-trans RA (Sigma; final concentration of 2 × 10−6 M). After the culture ...
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bioRxiv - Cancer Biology 2021Quote: ... at 30 °C The lysate solution was added 2.5 μL 800 mM tris(2-carboxyethyl)phosphine (ALDRICH®) and 5 μL 100 mM iodoacetamide (SIGMA®) to in PCT-Micro tube to dilute into a final concentration of 10 mM and 40 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one received henceforth 2 mg/ml DOX (BelaDox, Bela-pharm) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce TCF7L1 re-expression (DOX (+)) ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one was henceforth treated with 2 mg/ml DOX (Beladox, Bela-pharm, Germany) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce an in vivo re-expression (DOX (+)) ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Genomics 2021Quote: ... PTA was carried out by first further lysing the cells after freeze thawing by adding 2 μL a prechilled solution of a 1:1 mixture of 5% Triton X-100 (Sigma-Aldrich) and 20 mg/ml Proteinase K (Promega) ...
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bioRxiv - Physiology 2022Quote: ... 117 NaCl, 10 KCl, 1 MgCl2, 10 creatine, 20 taurine, 5 adenosine, 2 L-carnitine, 10 glucose, 10 HEPES [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2022Quote: ... by plating them on NB agar medium (2% glucose, Ade, Ura) supplemented with different concentrations (5-50mM) of 3-aminotriazole (3AT; Sigma-Aldrich) histidine inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed four times in 1X PBS for 5 minutes and blocked with 2% Normal Donkey Serum (Millipore S30-100ml) before incubation with secondary antibodies in 1X PBS ...
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bioRxiv - Neuroscience 2020Quote: ... 1X penicillin/streptomycin and anti-mitotic reagents (20 μM 5-fluoro-2-deoxyuridine and 20 µM uridine, both from Sigma-Aldrich). The medium replacement removed the electroporation buffer and dead cells.
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bioRxiv - Cell Biology 2022Quote: ... as well as the role of myosin II/RhoA in creating the refractory nature of the retracting losing front to receptor inputs (Fig. 5 and Supplementary Fig. 2) we pre-treated cells with either 50 μM nocodazole (Sigma Aldrich) or 10 μM Υ-27632 (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... plated in 96-well at a concentration of 2×105 cells/well and cultured for 72 h with medium alone or with OVA V (5 μg/ml; Sigma-Aldrich). Their supernatants were analyzed for cytokine expression using the V-Plex proinflammatory panel 1 mouse kit (MesoScaleDiscovery ...
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bioRxiv - Bioengineering 2021Quote: ... a 95/5 L-lactide/DL-lactide copolymer (Corbion) 2% m v−1 solution in dry 1,4-dioxane (Sigma-Aldrich, 296309) was spin-coated at 3000 rpm for 30 s on 1.25 × 1.25 cm Corning® glass microslides (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... This was sonicated in a water bath at room temperature for 15 min before addition of 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and 50 mM chloroacetamide (Sigma-Aldrich) and incubated at 37 °C while shaking ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with 0.2% BSA three times for 5 min each and incubated with 1 μg/ml DAPI (Bisbenzimide Hoechst 33342 trihydrochloride, Sigma B2261) and secondary antibodies diluted 1:500 in 0.2% BSA for 1 h (Invitrogen donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... For induction of the RRM2 knockdown 2 mg/ml Dox (BelaDox, Bela-pharm, Germany) was added in drinking water containing 5% sucrose (Sigma-Aldrich) for ad libitum uptake (Dox (+)) ...
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bioRxiv - Cell Biology 2021Quote: L4 stage or young adult worms were mounted on 2% agarose pads with 10 mM levamisole (CAS 16595-80-5; Sigma-Aldrich). A Zeiss LSM i880 microscope with Airyscan equipped with 40× Fluar objective (N.A ...
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bioRxiv - Biochemistry 2021Quote: ... chromatin isolated from 1–2 × 106 cells were incubated with 5 µg of mouse anti-FLAG (F1804, SIGMA-ALDRICH - RRID: AB262044) antibody pre-coupled to Protein A/G Sepharose ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were labelled with a PBS-Hoescht solution at 2 μg/mL for 5 min at RT and samples were finally mounted in mounting medium (F4680, Sigma Aldrich). Labelled cells and EVs were analysed with an inverted LSM 880 confocal microscope with airyscan (Carl Zeiss Ltd.) ...
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bioRxiv - Microbiology 2021Quote: HAE mucus was allowed to accumulate for one week prior to the application of 5 μl of 2 μm red-fluorescent (Cy3) polystyrene microspheres (1:50 dilution, Sigma-Aldrich) to the apical chamber of the transwell ...
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bioRxiv - Neuroscience 2020Quote: ... To inhibit the proliferation of non-neuronal cells a mitotic inhibitor 5-fluoro-2’-deoxyuridine (FDU, final concentration 10 μM, Sigma-Aldrich) was added with the change of the medium.
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bioRxiv - Animal Behavior and Cognition 2021Quote: At the end of the treatment sessions mice were subjected to intraperitoneal injections of BrdU (5-bromo-2-deoxyuridine, Sigma-Aldrich) at 50 mg/kg once a day during 4 days to label adult-generated progenitor cells in the DG of the hippocampus ...
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bioRxiv - Microbiology 2021Quote: ... and used to spinfect H1299-H2AZ at 1000 rcf for 2 hours at room temperature in the presence of 5 μg/mL polybrene (Sigma-Aldrich). ACE-2 transduced H1299-H2AZ cells were then sub-cloned at the single cell density in 384-well plates (Greiner Bio-One ...
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bioRxiv - Cancer Biology 2020Quote: ... were loaded with 30 – 50 µg protein in 5X SDS loading buffer (250 mM Tris-HCl, pH 6.8, 10% SDS, 30% glycerol, 0.02% bromophenol blue [Sigma-Aldrich, B8026], 5% 2-mercaptoethanol [Sigma-Aldrich, M6250]) after heating the samples for 5 min at 95°C ...
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bioRxiv - Cell Biology 2021Quote: ... at a density of 2×104cells/cm2 in RHB-A medium supplemented with 5 ng/ml murine bFGF (Sigma, SRP4038-50UG). Medium was changed again every 2 days for the next 3 weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... if fixed in 95% MeOH/5% GAA washes were done with PBDT (1XPBS/1%BSA/2%DMSO [dimethyl sulfoxide, Sigma Aldrich D8418]/0.5%Tween ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with 15 μL Fluoromount (Sigma F4680) for image acquisition ...
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bioRxiv - Pathology 2022Quote: ... The cultured C3H/10T1/2 cells were infected with 5 ml of retroviral supernatant for 48 h with 10 µg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... For immunohistochemical analysis of bronchiolar epithelium paraffin sections were first blocked with 5% goat serum and 2% fish gelatin (Both from Sigma-Aldrich) at 25 0C for 45 mins ...
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bioRxiv - Microbiology 2022Quote: ... difficile strains were routinely grown in BHIS broth or on BHIS agar supplemented with 2-5 μg ml-1 thiamphenicol (Sigma-Aldrich) as needed [58] ...
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bioRxiv - Neuroscience 2021Quote: ... coupled to an electrochemical detector (Decade Lite-Antec Scientific) with a 5-μm Spherisorb ODS-2 reversed-phase column (Sigma-Aldrich) and the results were expressed as norepinephrine concentration in ng/g of tissue.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then treated for 15 min up to 4 h at 37°C with 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, #M5655-1G) in 5 mM PBS ...
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bioRxiv - Microbiology 2020Quote: ... We grew donor strains in 96-well deep-well plates (1-mL cultures in TY medium in 2-mL wells) for at least 5 h shaking at 37 °C with a Breath-easy (Sigma-Aldrich) film covering the plate ...
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bioRxiv - Microbiology 2021Quote: ... For blocking non-specific sites cells were incubated 30 minutes in PBG (5% BSA, 2% gelatin from cold water fish skin, from Sigma-Aldrich) and then stained for 2 till 16 hours with rabbit anti-IFITM3 polyclonal antibody (1:200 dilution from proteintech catalogue number 11714-1-AP ...