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Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The recording chamber was constantly perfused (2-3 ml/min) with aCSF and 100 μM picrotoxin (Sigma Aldrich) was added to block inhibitory synaptic transmission ...
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bioRxiv - Genetics 2020Quote: ... cells were UV-irradiated (60 J m-2) through isopore polycarbonate membranes containing 3-μm-diameter pores (Millipore). For CPD immunodetection ...
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bioRxiv - Cell Biology 2020Quote: ... Both the CS and cantilevers were washed with acetone and functionalized using 2% (v/v) 3-aminopropyltriethoxysilane (Sigma) dissolved in acetone ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Cell Biology 2020Quote: The cantilevers were functionalized with either bovine serum albumin (BSA, 3%, 2 h, 20 °C, Sigma-Aldrich, A7906), Bis-NHS-polyethylen glycol (5 % w/w ...
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bioRxiv - Microbiology 2022Quote: ... media were aspirated and cells viability was determined by addition of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) at a final concentration of 0.5 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was resuspended and incubated for 30 min at 4 °C in buffer B (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Molecular Biology 2022Quote: Cell growth was monitored by cell counting and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using MTT cell growth assay kit (#CT02, Millipore). FL or ΔN-Drosha cells (1×105 ...
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bioRxiv - Biochemistry 2022Quote: ... D-glucose N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, USA ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS and 10% 5mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (SIGMA) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... or MG132 using a MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-based in vitro toxicology kit (TOX1-1KT, Sigma). 10 µl MTT solution was applied to cells at the end point of the experiment and incubated for 4 hours ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitors (prepared in-house according to Phosphatase inhibitor cocktail 1, 2 and 3 from Sigma-Aldrich)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The MTT [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyl-tetrazolium Bromide] dye (M2128) was obtained from Sigma Aldrich. Triple distilled water (Millipore ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-3 synthetic gRNAs per gene targeting exonic regions were designed using CCTop54 and ordered from Sigma-Aldrich (spyCas9 sgRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were counterstained after 3 washes of PBS with 0.3 μg/mL 4,6-diamidino-2-phenylindole (Sigma-Aldrich). Images including Z-stacks were acquired on a Leica SP8 gSTED with an objective with x63 magnification ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined after 72 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (#M5655, Sigma) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 3 PBS washes were performed and blocking was done with 2% Bovine Serum Albumin (Sigma Aldrich,#A7906-100G) and 5% goat serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The MTT [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyl-tetrazolium Bromide] dye (M2128) was obtained from Sigma Aldrich. Triple distilled water (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were washed 3 times in PBS before addition of 2% Triton X– 100 (Sigma-Aldrich) PBS solution to lyse the cells for enumeration of the intracellular bacteria ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... the PDMS stamp is placed on a silanized coverslip pretreated with 2% (3-Trimethoxysil) propyl-methacrylate (Sigma #M6514)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: PESTANAL® analytical grade tefluthrin (2,3,5,6-tetrafluoro-4-methylbenzyl 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylic acid) was purchased from Sigma Aldrich. Tefluthrin stock solutions were prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Bioengineering 2024Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and collagenase type 1 was purchased from Sigma-Aldrich. RNA extraction kit (Iran) ...
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bioRxiv - Neuroscience 2024Quote: ... slides were cleared in Xylene (2 × 3 min) and coverslipped with DPX mounting medium (Cat# 44581, Sigma-Aldrich). Sections were photographed with an epifluorescence microscope (Model BX51 ...
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bioRxiv - Cell Biology 2024Quote: ... The number of viable cells was quantified using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; M2128, Sigma) assay at 0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Phase 3 (S3, 2 days): S3 medium + 50 ng/mL FGF7 + 1 μM retinoic acid (Sigma, Cat# R2625) + 0.25 μM SANT-1 (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... Growth inhibition was assessed with a MMT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma Aldrich). Optical density was measured at 595 nm and 650 nm with a microplate spectrophotometer (Multiskan ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Cancer Biology 2020Quote: ... and Hs 766T were plated at a density of 2×104 cells/cm2 and were treated for six days with 100 μM 5-aza-2’deoxycytidine (Sigma Aldrich, St. Louis, MO) or for 24 hours with 50 nM Trichostatin A (Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... The cell pellets were suspended in 5 ml pure water at 4 ℃ with 0.4 % TritonX-100 (Sigma-Aldrich), 2 μl DNAase Ⅰ(Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was pipetted from the pellet and 20 mM 5′-(4-Fluorosulfonylbenzoyl)adenosine hydrochloride (FSBA; Sigma Aldrich) was added to the supernatant ...
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bioRxiv - Genetics 2022Quote: ... ∼100 animals per condition were transferred to 4-5 NGM plates containing 7.5mM t-booh (Luperox TBH70X, Sigma). Worms were scored for survival every 2 hours ...
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bioRxiv - Genetics 2021Quote: ... After blocking with 4– 5% skim milk (MBcell, South Korea) in TBST including 25 mM Tris (Sigma, USA), 140 mM NaCl (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Primary antibodies were applied overnight at 4°C in a blocking buffer of 5% donkey serum (Sigma-Aldrich) at the following concentrations ...
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bioRxiv - Immunology 2021Quote: ... in complete media containing 10 U/mL rhIL-2 with or without 10 or 20 uM 6-(4-Chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO) (Sigma-Aldrich); an equal volume of DMSO served as the negative control.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... washed with PBS and incubated for 4 hours in complete DMEM/F12 containing 5 µg/ml cycloheximide (Sigma) to allow receptor recovery and prevent de novo protein synthesis18 ...
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bioRxiv - Molecular Biology 2022Quote: U2OS cultured at 37 °C/5% CO2 in 15 cm2 dishes supplemented with 4 μg/ml Doxycycline (Sigma) for 48 hours to induce exogenous SAE1/SAE2 expression ...
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bioRxiv - Microbiology 2022Quote: ... RNA (5 μg) was enzymatically hydrolyzed for 4 h at 37 °C with benzonase (99% purity, Novagen 70664), bacterial phosphatase (ThermoFisher 18011015 ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum cultures (4-8 hpi) were treated with apicoplast inhibitors (either 5 µM clindamycin (Sigma-Aldrich, PHR 1159) for > 50 times the delayed death IC₅₀ ...
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bioRxiv - Microbiology 2024Quote: ... and 12,000 × g for 5 minutes at 4°C using a Sigma 3K30 centrifuge (Sigma Laborzentrifugen GmbH, Germany), and the supernatant was collected ...
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bioRxiv - Cell Biology 2023Quote: ... skin samples were digested at 4°C overnight in 5 mg/mL dispase II solution (#D4693 Sigma-Aldrich) in HBSS (#H8264 Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with an Amicon Ultra-4 centrifugal filter with a 5 kDa molecular weight cutoff (UFC8003, Merck Millipore), snap frozen and stored at -80 °C either directly (used for cryo-EM ...
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bioRxiv - Genetics 2023Quote: ... The synthesized sgRNAs were extracted with phenol (pH 4–5):chloroform:isoamyl alcohol (125:24:1) (Sigma, Cat#P77619), precipitated with isopropanol ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then bleached overnight at 4 C in methanol containing a 5% of hydrogen peroxide (Sigma-Aldrich). Rehydration was done by incubating the samples in methanol 60% ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then bleached overnight at 4 C in methanol containing a 5% of hydrogen peroxide (Sigma-Aldrich). Rehydration was done by incubating the samples in methanol 60% ...
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bioRxiv - Immunology 2023Quote: ... samples were cut into thirds and incubated overnight at 4°C in 5 mg/mL Dispase II (Sigma) in Hanks Buffered Salt Solution (HBSS) ...
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bioRxiv - Developmental Biology 2023Quote: ... pregnant Sprague-Dawley rats received 100mg of nitrofen (2,4-Dichlorophenyl 4-nitrophenyl ether; Sigma CAS#1836-75-5) in 1mL of olive oil at E9.5 to induce fetal pulmonary hypoplasia ...
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bioRxiv - Neuroscience 2024Quote: ... at 1:2000 dilution in 5% BSA in TBST at 4 °C overnight and VCL (Sigma-Aldrich, V9131) as the loading control at 1:375,000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... blocked in FACS buffer (PBS + 4% FBS v/v) + 5% (v/v) normal goat serum (Sigma, CAT#G9023) for 10 min at 25 °C ...