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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... After permeabilization with 0.5% Triton X-100 in PBS for 10 min at 4°C and blocking with 3% bovine serum albumin (BSA) (Wako) in PBS with 1% DAPI (Sigma-Aldrich) at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were washed in PBS at least 3 times for 4 hrs and then incubated in Histodenz-Triethanolamine solution (about 80% Histodenz D2158 (Sigma-Aldrich), 11.5 mM PB ...
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2020Quote: ... N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 0.3 µg/0.5 µL/hemisphere; n = 9; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Microbiology 2020Quote: ... glutaraldehyde buffered with 3× PHEM (120 mM PIPES, 50 mM HEPES, 20 mM EGTA, 4 mM magnesium chloride, all from Sigma-Aldrich) (Montanaro et al ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated for 24 hours with either 1 mM 3-Hydroxy-1,2-dimethyl-4(1H)-pyridone (Deferiprone/DFP, Sigma-Aldrich, 379409), or incubated in Earl’s balanced salt solution (EBSS ...
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bioRxiv - Cell Biology 2022Quote: ... thereafter differentiations were kept in RB- without small molecule inhibitors until day 3 (d3) before the WNT inhibitor IWR-1 (4 μM, Sigma, #I0161) was added for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating VTA brain sections were incubated 1h at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% Bovine Serum Albumin (BSA, Sigma A4503) and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections were then permeabilized overnight at 4°C by gently rocking in blocking buffer comprising 3% bovine serum albumin (Sigma Aldrich), 1% saponin (Sigma Aldrich ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Cell Biology 2023Quote: ... The AAV was concentrated by centrifugation at 2000g for 3-5min at 4°C using an 100k Amicon Ultra Centrifugal Filter Unit (Millipore #UFC810024). After centrifugation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... diluted in corn oil (CO, used as vehicle) at 3 mg/kg for 4 days while CO mice received corn oil only (Sigma Aldrich). One cage of n=6 mice per group was used ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... solution for 3 hours at 4°C under gentle agitation and then washed it three times in TBS/Tween20 (TBST) buffer (Sigma Aldrich). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Sub culturing was done once in 3-4 days and involved pelleting and washing cells with PBS followed by incubation with accutase (Sigma-Aldrich) and repeated pipetting in NSM medium to dissociate the spheroids ...
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bioRxiv - Neuroscience 2022Quote: ... VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... GV oocytes were collected from the ovary of 3-4 weeks old C57BL/6 females 48 h after PMSG injection and then were incubated in M2 medium (Sigma, #M7167) supplemented with 10 μM Cilostamide (TargetMol ...
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bioRxiv - Molecular Biology 2024Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous or 3-4 days administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Genetics 2023Quote: ... at 4°C for 1 h followed by overnight incubation with 3 μL of the following antibodies: H3K4me2 (Millipore 07-030), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Cancer Biology 2023Quote: ... IFI44L (#1: TRCN0000167757; #3: TRCN0000242541) or ISG15 (#4: TRCN0000007423)) in the presence of X-tremeGENE HP DNA Transfection Reagent (Sigma Aldrich). Five shRNAs per gene were tested for knockdown efficiency ...
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bioRxiv - Systems Biology 2024Quote: ... morphine paradigm were communally housed (3-4 mice per cage) and had access only to water supplemented with either sodium butyrate (Sigma-Aldrich) or pH-matched (9.30 - 9.50 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was subjected to an additional centrifugation step (10000g, 10 min at 4℃) and concentrated using a 3 kDa Amicon Ultra centrifugal filter (Millipore, USA). The protein concentration in each sample was determined by DC protein assay (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 80 µL of the PG sample was digested for 3 h at 37°C with 4 µg of lysozyme (Sigma-Aldrich) and then ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenized tissue was centrifuged at 16,000 g for 45 min at 4 °C and the remaining supernatant was spun in 3 kDa cut-off Amicon® centrifugal filter tubes (Merck Millipore) at 14,000 g for 90 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with 3 % BSA for 20 min and incubated with 4 µg anti-FLAG monoclonal antibody (Sigma SAB4200119) for 20 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The bone marrow-depleted ankles were cut into 3-4 mm sized tissue pieces and incubated with digestion media containing 2.4 mg/ml hyaluronidase (Sigma-Aldrich, #H3506), 1 mg/ml collagenase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... These double transgenic animals were separated into 4 groups (n = 3) and injected with a single dose of 4OH-Tx (Sigma-Aldrich). Tissue was collected at 10- ...
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bioRxiv - Neuroscience 2024Quote: ... sciatic nerves were harvested and fixed for 1 h in 4% paraformaldehyde (PFA) followed by 15 min immersion in a 3% sodium fluorescein (NaFlu, molecular weight: 376 Da; Sigma Chemicals) in saline solution 2 ml of Evans blue albumin (EBA ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating VTA brain sections were incubated for 3 hours at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Biophysics 2024Quote: ... cells were exposed to an overnight incubation at 4°C with 3 mL of a 0.2% gelatin solution (Sigma-Aldrich, #G2500). Primary antibody treatment involved the use of anti-TNFR1 rabbit antibody (ABclonal ...
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bioRxiv - Cell Biology 2021Quote: ... 1uM each of gRNA (IDT) were mixed with spCas9 at 3:2 ratio in embryo grade water (W1503, Sigma) and injected into wild type C57BL6 zygotes at one-cell stage ...
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with Oil Red O working solution (3:2 Oil Red O: ultrapure water) for 20 minutes (Sigma O1391), and washed with 5 changes of ultrapure water.
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was determined using the 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, Milan, Italy) according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2020Quote: ... Viability was evaluated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric assay (Sigma, #M5655).
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bioRxiv - Microbiology 2020Quote: Biofilms were set up as described previously except they were incubated at 37°C in either 0.03% CO2 or 5% CO2 for 48h in RPMI-1640 containing varying concentrations of the Fe2+ chelator 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p’-disulfonic acid monosodium salt hydrate (Ferrozine – Sigma-Aldrich, 160601) or the Fe3+ chelator Deferasirox (Cambridge Bioscience ...
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bioRxiv - Biophysics 2022Quote: ... Both the CS and cantilevers were washed with acetone and functionalized using 2% (v/v) 3-aminopropyltriethoxysilane (Millipore Sigma) solution dissolved in acetone ...
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bioRxiv - Systems Biology 2022Quote: ... and then a certain amount of culture (2~3 mg dry weight) was filtrated through a biomass filter (Durapore 0.45 μm, diameter 25-mm, Sigma). The filter with the cell pellet was immediately transferred to an aliquot of 4 mL of precooled extraction solution (−20°C ...
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bioRxiv - Physiology 2022Quote: Cell viability was measured by the colorimetric assay showing reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) as previously described (Denizot and Lang ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich) as described [29].
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bioRxiv - Biochemistry 2022Quote: ... Cholesteryl hemisuccinate (CHS, C6013) and 1-stearoyl-2- hydroxy-sn-glycero-3-phosphocholine (Lyso-PC) were purchased from Sigma. Brain phosphatidylinositol-4-phosphate (PI(4)P) ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of medium containing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (1 mg/mL) (Sigma-Aldrich) was added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... frozen tissue (from 2–3 mice or 1 rat) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... This assay relies on the reduction and conversion of yellow 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) reagent (#M2128, Sigma-Aldrich) into purple formazan salt ...
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bioRxiv - Genetics 2021Quote: ... 2-3 eyes from different zebrafish were sonicated in RIPA lysis buffer containing protease inhibitor cocktail (Sigma, Cat# P2714). Protein samples were mixed with loading buffer and boiled for 5 minutes at 95-100°C ...