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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation was performed at 4°C overnight with nutation in 1 ml chromatin aliquots with 3 ul of anti-FLAG M2 antibody (F3165, Sigma) and 20 ul of Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Percentage of GFP positive cell population was measured at day 3 or day 4 as initial time point using a Guava Easycyte HT instrument (Millipore). GFP% (mCherry% ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck ...
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bioRxiv - Physiology 2021Quote: ... The preparation was bathed in standard locust saline (in mM: 147 NaCl, 10 KCl, 4 CaCl2, 3 NaOH and 10 HEPES buffer; pH = 7.2; chemicals from Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... 2-3 mg of sNASP-FLAG-myc and sNASP-Strep-HA extracts were incubated for 3 hours at 4 °C degrees with 50 μl of anti-Flag M2 (A2220, Sigma) or Anti-TwinStrep Mag-Strep beads (2-4090-002 ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing eluted BfNFeoAB were concentrated at 4°C using a 15 mL Amicon 3 kDa molecular-weight cutoff (MWCO) spin concentrator (Millipore). Concentrated protein was then further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 1 hour in 3% BSA-TBS and incubated overnight at 4°C with the following primary antibodies: anti-phosphotyrosine 4G10 (Millipore), anti-phosphorylated SYK (pY525/526 ...
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bioRxiv - Developmental Biology 2022Quote: ... Prior to start of imaging 6.5 ml culture medium with 1 μg/ml 17alpha,20beta-Dihydroxy-4-pregnen-3-one (DHP, diluted in 100% Ethanol, Sigma) containing drug/Lysotracker was added on top of the solidified LMP agarose ...
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bioRxiv - Molecular Biology 2022Quote: ... were diluted in PBS together with 3 μg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma) and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and 3-4 min at room temperature with a pancreatin-trypsin mixture (2.5mg/ml pancreatin [P3292; Sigma Aldrich] and 22,5mg/ml trypsin in Thyrode’s solution pH 7.4) ...
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bioRxiv - Plant Biology 2023Quote: Samples were incubated with 20 µL of IS solution (155 µmol/L forchlorfenuron, 25.5 µmol/L 4-chlorphenylacetic acid, 5.16 mmol/L indole-3-acetic-2,2-d2 acid, Sigma-Aldrich, Steinheim, Germany) and diluted with an acetonitrile/methanol + 1% formic acid solution (4:1 ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was filtered at 300 × g for 3 minutes at 4°C through a 0.45-μm 96-well filter plate (Millipore, MSHVN4550) to remove protein aggregates ...
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bioRxiv - Immunology 2023Quote: ... Th17 cells were split (usually 1:3) and transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) in addition to 10 ng/ml IL-23 and 10 µg/ml anti-IFNγ antibody (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2023Quote: iLPC organoids formed after 48 hours post-seeding were fixed for 3 hours in 4% paraformaldehyde (PFA) in phosphate buffered saline (PBS) (Sigma), before a double-embedding process in 1.5% agarose gel then paraffin as described previously [13 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro excision of Nr3c1 exon 3 was achieved by treating primary myoblasts with 4-OH-tamoxifen (Sigma Aldrich, H7904).
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bioRxiv - Developmental Biology 2023Quote: ... in PBS at +4⁰C in the shaker and then 3-4 min with a pancreatin-trypsin (2.5 mg/ml pancreatin [P3292; Sigma Aldrich] and 22.5 mg/ml trypsin dissolved in Thyrode’s solution pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: Cells were fixed in 4% paraformaldehyde and permeabilized/blocked using PBS with 3% goat or donkey serum and 0.2% triton X-100 (Sigma Aldrich). Cells were incubated at 4°C overnight with primary antibodies against the following targets ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma] ...
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bioRxiv - Neuroscience 2024Quote: ... The rest of the sample was divided into three tubes and incubated overnight rotating at 4 °C with 3 μl of mouse anti-VE-cadherin antibody (MABT134, Millipore) or donkey anti-mouse IgG (A16013 ...
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bioRxiv - Neuroscience 2024Quote: ... The preparation was bathed in standard locust saline (in mM: 147 NaCl, 10 KCl, 4 CaCl2, 3 NaOH, 10 HEPES buffer; pH adjusted to 7.2; chemicals from Sigma-Aldrich) and grounded with a chlorided silver wire in the abdomen ...
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bioRxiv - Biophysics 2024Quote: ... fluorescent labelled tRNAGln and tRNATrp were isolated instead using 5’ - CAA AAA CCG GTG CCT TAC CGC TTG GCG - 3’/3AmMO/ and 5’ - TTT GGA GTC GAA AGC TCT ACC ATT - 3’/3AmMO/ DNA oligos, respectively, with 3’ amino-modifications (3AmMO, IDT) conjugated to N-Hydroxysuccinimidyl (NHS)-Sepharose 4 Fast Flow beads (H8280, SIGMA).
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bioRxiv - Cell Biology 2024Quote: ... Sftpc-CreERT2 and Rosa26R-CAG-lsl-tdTomato (R26R-tdTomato) mice were given 3-4 doses of tamoxifen (100 mg/kg, i.p.; Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... the membrane was immunoblotted for 16 hours at 4 °C in blotting buffer (3% BSA in TBS with 0.1 % Tween-20 (Sigma, P7949) with 1 μg/ml rabbit anti-MTNR1B (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (L’Isle-d’Abeau-Chesnes, France). Antibodies against phospho-IκBα Ser32 (#2859) ...
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bioRxiv - Neuroscience 2021Quote: Prior to training rats were randomly assigned to receive either sham surgery or intracranial bilateral lesions to the region of interest under 2-3% isoflurane anaesthesia with local application of bupivacaine (2mg/kg s.c. at 0.8ml/kg; Sigma) at the incision site ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %). The treatment was counterbalanced between trials ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %).
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bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Metabolic activity was measured by adding 10 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 10 mg/ml, Sigma). After four hours of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.1% SDS with cOMPLETE™ Mini protease inhibitor cocktail (#11836153001) and phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Cell Biology 2020Quote: ... or erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA; 100μM, E114-25MG, Sigma-Aldrich, Saint Louis, MI, USA) prior to fixation with 2% PFA (28906 ...
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bioRxiv - Plant Biology 2021Quote: ... The worms were washed 2–3 times with PBS-T and then stained with 0.125% thioflavin-S (Sigma) in 50% ethanol for 2 min and then de-stained sequentially in ethanol (50% ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were neutralized with 3% ammonium hydroxide and extracted with 2 ml of water-saturated diethyl ether (Sigma), vortexed and centrifuged at 2,000 x g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... This plasmid was transformed to Novagen’s Rosetta™ 2(DE3)pLysS competent cells (Millipore Sigma, Cat#71403-3) for rhFGF10 expression ...
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bioRxiv - Cancer Biology 2022Quote: ... 25mM Tris) supplemented with protease and phosphatase inhibitors (Protease inhibitor cocktail, Phosphatase inhibitor cocktail 2/3, Sigma Aldrich) for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 3 the cell culture media was replaced 1-2 hours before imaging with RPMI media (Sigma-Aldric R8755) supplemented with B27 ...
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bioRxiv - Bioengineering 2022Quote: ... The activated membranes were immediately submerged in a solution of 2% bis[3-(trimethoxysilyl)propyl]amine (Sigma-Aldrich) in 1% water and isopropyl alcohol ...
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bioRxiv - Bioengineering 2022Quote: ... slides were stained in Phosphomolybdic Acid / Phosphotungstic Acid mix (Sigma-Aldrich, product number HT15-3 and HT15-2) for 7 minutes and in Aniline Blue Solution (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Silicon chips were functionalized with a 2% (3-aminopropyl) triethoxysilane (APTES, 98%, Sigma-Aldrich, St. Louis, MO, USA) solution to attach complexes to the surface by interactions with free amine groups ...
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bioRxiv - Neuroscience 2021Quote: ... and at 3 days in-vitro (DIV) glial proliferation was inhibited with 2 μM cytosine arabinoside (Sigma, C6645).
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bioRxiv - Immunology 2021Quote: ... Subsequently cells were washed 3 times with PBS before being treated with 2% saponin (250 µL/well) (Sigma) in distilled water and incubated for 15 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately1-2 μL of endotoxin-free DNA (1-3 mg/ml) diluted in PBS/0.025% Fast Green (SIGMA) was injected into the lateral ventricles of the forebrain using heat-pulled glass micropipettes (Drummond) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell viability was evaluated by using the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay (Sigma). Non-treated cells were used as control ...