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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 3% SDS (Sigma-Aldrich, 151-21-3), 25 mM β-glycerophosphate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... ultracel-3 (>3 kDa cutoff) concentrators (Millipore). 30 µl of concentrate was loaded for western blot analysis.
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bioRxiv - Bioengineering 2023Quote: ... The 1.5 ml resuspended RBCs were cultured in 50 ml HPS containing 2 mM CaCl2 and 2 µM calcium ionophore-4-bromo-A23187 (C7522, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in T75 flasks in a 5% CO2 incubator at 37°C for 16 h ...
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bioRxiv - Microbiology 2020Quote: ... treated 3T3 mouse fibroblast feeder cells and grown until 70% confluency in the 3:1 mixture of Ham’s F12/DMEM media (HyClone) supplemented with 5% FBS (Sigma), 24 μg/mL adenine (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times and secondary staining was done in permeabilization buffer supplemented with 5% Donkey serum (Sigma) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... gRNA sequence: 5’-ACTTGGCCAAGGAGTACGG-3’) in line with GFP from the (p04) U6-gRNA:CMV-eCas9-2a-tGFP plasmid (Sigma). One day later ...
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bioRxiv - Microbiology 2021Quote: Cells transfected with NL-43 or pNL4-3-△5’ were lysed using freshly made lysis buffer (0.5% Igepal CA-630, Sigma; 50 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... the slides were washed 3 times for 5 mins while gently shaking at RT with PBS + 0.1% Tween (Sigma) and stained for 90 mins in blocking buffer with a combination of secondary antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... Br-3’,5’-cAMP (B5386) and Br-adenosine (B6272) were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Br-2’,3’-cAMP was purchased from Biolog (Biolog Life Science Institute ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed twice for 10-m in 1x PBS and then incubated for 1-hr in a blocking buffer containing 3-5% Normal Goat Serum (Jackson Immuno Research, 005-000-121) and 0.01-0.03% Triton-X (Sigma, X100), made up in 1x PBS ...
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bioRxiv - Neuroscience 2022Quote: ... N2a NM-HAagg (EP) donor cells were treated for 3 d with methyltransferase inhibitors 5-Azacytidine (Aza) (Sigma-Aldrich), Decitabine (Dec ...
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bioRxiv - Immunology 2020Quote: ... 50 μl of antibody exposed to hemin or hemin in PBS only were mixed with 150 μl of reaction buffer (0.15 M citrate-phosphate buffer pH 5) containing 0.9 mM of 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma-Aldrich) and 6 mM H2O2 ...
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bioRxiv - Cell Biology 2020Quote: ... and gravity-sedimented for 3-5 min onto coverslips coated with poly-D lysine (Sigma-Aldrich, catalog number P1024), until cells became in contact with the glass ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were broken by vortexing (3 cycles, 5 min each) using 0.2 ml of glass beads (425-600 μm; Sigma)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1.47 mM KH2PO4 supplemented with 5 mM DTT and concentrated to ∼10-20 mg/mL using 3 kDa Millipore concentrator and stored at -80 °C.
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times for 5 min and incubated with anti-mouse-HRP antibody (0.2 μg/ml – Millipore) for 1 h in TBST ...
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bioRxiv - Plant Biology 2020Quote: ... The bottom of the tilted plates was filled with 13 ml of 0.5 M glucose solution for glucose supplementation experiments or 100 μM 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU, Sigma®) for sugar starvation experiments ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Cre reverse primer 5’-GGT TTC CCG CAG AAC CTG AA-3’ (both primers were purchased from Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed with IP lysis buffer and incubated with 5 mg ml−1 3× FLAG peptide (Sigma–Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was resuspended in 3 mL Nuclear Permeabilization Buffer (1X PBS, 5% Bovine Serum Albumin, 0.2% IGEPAL CA-630 (Sigma), 1 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... 100 μg/mL streptomycin and 1 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic sodium monophosphate salt (dbcAMP) (Sigma®). Cells were maintained with the differentiation medium at 37 °C in 5% CO2 for 3 days.
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bioRxiv - Neuroscience 2022Quote: ... Passive dextran permeability was measured by culturing iBECs on Ø 0.4 μm pore polyester or polycarbonate Transwell inserts and cells were exposed to 0.5 mg/mL fluorescein isothiocyanate (FITC)-conjugated 3 – 5 kDa dextran (Sigma) for 24h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with DPBS for 5 min and blocked in 1% BSA (Sigma-Aldrich, cat# A4503) in PBS-T (DPBS with 0.05% Tween 20 ...
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bioRxiv - Plant Biology 2024Quote: ... They were then resuspended in 10 mM MES-KOH buffer (pH 5.7) with 10 mM MgCl2 and 100 mM acetosyringone (49-hydroxy-3′,5′-dimethoxyacetophenone; Sigma) to achieve a final OD600 of 0.5 ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed to add the 3XFlag tag to the N terminus of ZBP1 cDNA at the 5’ Not1 site and 3’ Sal1 site to facilitate cloning into expressing vector p3XFlag-CMV-7.1 (Sigma). The resulting plasmid containing the 3XFlag-ZBP1 was further subcloned in the Bst1 and BamHI sites of the vector pLVX-EF1α-AcGFP1-C1 (Takara ...
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bioRxiv - Neuroscience 2022Quote: ... The dopamine- synthesis inhibitor 3-Iodo-L-tyrosine (3IY; CAS: 70-78-0, Sigma Aldrich; concentration: 5 mg/ml) was added to samples of 2 ml yeast solution ...
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bioRxiv - Microbiology 2022Quote: ... cultures were pelleted by centrifugation at 1,600 rpm for 3 min and resuspended in a 10× volume of 5% sorbitol (Sigma), followed by incubation at 37°C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... by dispensing individual cells in 5 nL drops directly into 3 µL ice-cold single cell lysis buffer (scLB, 0.134% Triton X-100 [Sigma] ...
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bioRxiv - Neuroscience 2023Quote: ... 3-5 organoids per condition were dissociated by incubation with a solution of 0,5 mg/ml Collagenase/Dispase (Sigma) + 0,22 mg/ml EDTA (Euroclone ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific], 0.4 μL/mL hydrocortisone [Sigma] ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were again washed 3 × 5 min with PBS + 0.1% Tween 20 and incubated with Classico/Crescendo Western HRP substrate (Millipore-Sigma) for 2 min ...
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bioRxiv - Genetics 2023Quote: ... or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence) (5’-CTGGTGGATAAGGATTTGGAT-3’ clone NM_003108.3-1235s1c1; Sigma-Aldrich) were created by transfection into HEK293T packaging cells ...
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bioRxiv - Physiology 2024Quote: ... The PDMS scaffold surface was activated oxygen plasma and then treated with a 5% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) solution in methanol for 45 min ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... mice were intravenously injected with FITC-dextran (100µL of 3-5% w/v in 0.9% saline; 70kDa, Sigma #46945) or Texas Red dextran (100µL of 3% w/v in 0.9% saline ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS (3 times x 5 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...
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bioRxiv - Plant Biology 2024Quote: ... seeds were surface sterilised with ethanol and germinated on ½ x Murashige and Skoog (MS) medium with Gamborg’s B-5 vitamins (Duchefa) and 3% (w/v) sucrose (Sigma) containing 0.8% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... Primary roots were imaged 3-5 days after germination on half-strength Murashige and Skoog medium (MS, Sigma Aldrich) plates with 1% w/v sucrose and 0.8% Difco-agar (Appleton Woods ...
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bioRxiv - Bioengineering 2024Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific], 0.4 μL/mL hydrocortisone [Sigma] ...
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bioRxiv - Bioengineering 2024Quote: ... Primary cells were cultured in basal medium ((DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific], 0.4 μL/mL hydrocortisone [Sigma] ...
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bioRxiv - Immunology 2020Quote: ... and S100A9 (rA9) were concentrated using an Amicon Ultra-4 centrifugal filter device with a 3 kDa molecular-weight cutoff (Millipore). The primary sequence ...
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bioRxiv - Cell Biology 2020Quote: ... and Ypt1-7xHis were mixed in a 1:3 molar ratio and incubated overnight at 4°C with calf intestinal alkaline phosphatase (Sigma) to hydrolyze nucleotide ...
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bioRxiv - Immunology 2021Quote: ... Histone neutralisation experiments were performed via intraperitoneal injection with dialysed and combined a-Histone 3 and a-Histone 4 antibodies (Merck Millipore) or control polyclonal rabbit IgG (BioXCell) ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Plant Biology 2020Quote: ... plants were incubated overnight at 37°C in GUS buffer (3 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronide [Duchefa Biochemie, Haarlem, The Netherlands], 0.1% v/v Triton X-100 [Sigma, Steinhaim ...
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bioRxiv - Biophysics 2021Quote: ... was also placed into 7 M Guanidium HCl using three successive concentrations and dilutions using a centrifugal concentrator (3 K MWCO, Amicon Ultra-4, Millipore), and diluted to 2 mg/mL ...
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bioRxiv - Biophysics 2021Quote: ... Free dye was removed from the labeled proteins by successive concentrations and dilutions using a 3 K MWCO centrifugal concentrator (Amicon Ultra-4, Millipore). The labeling efficiency was computed by measuring the concentration of the protein and Cy3 dye using their absorbances at 280 nm and 550 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) twice with 300 μL of Elution buffer (Lysis buffer A with 1 mg/mL 3 × FLAG peptide (Sigma)) ...
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bioRxiv - Neuroscience 2021Quote: Synaptosomes were prepared from the striatum or cortex of VGLUT1venus or DAT-Cre eGFP-expressing mice by homogenization in 1ml of ice-cold isosmolar buffer (0.32M sucrose, 4 mM HEPES pH7.4, protease inhibitor cocktail Set 3 EDTA-free (EMD Millipore Corp.)) ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated 1-3 overnights at 4°C in the following primary antibodies: goat anti-choline acetyltransferase (1:200; Millipore), rabbit anti-substance P (1:500 ...