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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Human melanoma cells were seeded in 6-well plates with 0.1-1 mL lentivirus in a final volume of 3 mL with 4 µg/mL polybrene (Millipore). Cells were spun at 1,000 x g for 2 h at 30 ºC ...
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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bioRxiv - Neuroscience 2022Quote: A model of hyperalgesic priming was used to induce chronic widespread pain in mice with consecutive injections of carrageenan into the right gastrocnemius muscle 4 days apart (i.m. 3% 30μl, Sigma Aldrich). Control mice only received one injection on D0 (30).
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bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Developmental Biology 2020Quote: ... were post-fixed with 4% para-formaldehyde and blocked with PBS solution containing 0.3% Triton-X 100 and 3% normal Donkey serum (Sigma). Antibody dilution buffer consisted of PBS containing 1% BSA and 0.3% Triton-X 100 ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
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bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
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bioRxiv - Biochemistry 2024Quote: ... Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP, Sigma Aldrich) dissolved in 100 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were concentrated to ∼ 3-4 mg mL-1 using a 100 kDa MWCO centrifugal concentrator (Millipore Sigma) for cryo-EM sample preparation.
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bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
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bioRxiv - Bioengineering 2023Quote: ... they were incubated with 4 mg/mL of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma-Aldrich, Schnelldorf, Germany) dissolved in 100 mM 2-(N-morpholino ...
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bioRxiv - Microbiology 2023Quote: Infected S10-3 cells seeded onto coverslips were fixed in 4% PFA and permeabilized in 0.1% Trion-X100 (Sigma). For co-detection of RNA and capsid ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... and then blocked for 3 hr at room temperature or overnight at 4 °C in BSA (Sigma-Aldrich A2153) 3% in PBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... the matrices were incubated with 4 mg/mL N-(3- dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma‒Aldrich, Schnelldorf, Germany) dissolved in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Neuroscience 2021Quote: ... and antimitotic agents 5-Fluoro-2′-deoxyuridine and Uridine (FdU/U) (Sigma-Aldrich, MO, USA) were added at a concentration of 10μM to control glial proliferation ...
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bioRxiv - Genetics 2021Quote: SNU-398 cells were treated with 2.5 μM of 5-aza-2’-deoxycytidine (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg/ml insulin (#I9278) and 2 × 10−11 M liothyronine (all Sigma-Aldrich, #T6397). HEK293T cells (a gift from Noor Gammoh ...
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bioRxiv - Microbiology 2020Quote: ... and blocked for 5 min in PBS buffer containing 2% bovine serum albumin (BSA, Sigma). Incubation with primary antibodies in PBS buffer ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-α-tubulin (1:2,000; clone B-5-1-2, Sigma-Aldrich, cat. # T5168) and mouse anti-MIC2 (1:2500 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-Bromo-2′-deoxyuridine (10280879001) and D-Luciferin (L9504-5MG) was purchased from Sigma-Aldrich. All plasmids used in this study were listed in supplementary Table 1 and will be donated to Addgene.
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bioRxiv - Pathology 2022Quote: ... 15-30 μg protein per sample were denatured with 5 % vol/vol 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... all assays were scored after 6 days and included 5-fluoro-2’-deoxyuridine (FUdR; SIGMA) to prevent the appearance of the next generation of larvae that would complicate the experiments [55] ...
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bioRxiv - Immunology 2022Quote: ... was modified with 5-Norbornene-2-acetic acid succinimidyl ester (Nb-NHS) (Sigma Aldrich #776173) using a ratio of 1g Nb-NHS ...
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bioRxiv - Biophysics 2022Quote: ... followed by 2 h in 5 μg/mL laminin (Sigma-Aldrich, L2020, diluted in PBS). Following explant plating ...
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bioRxiv - Microbiology 2022Quote: ... The cells were sequentially labeled with 30 μM 5-iodo-2′-deoxyuridine (IdU) (Sigma-Aldrich) at 37°C for 4 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Other reagents used in this study included 5-chloro-2’-deoxyuridine (CldU, Sigma-Aldrich C6891), 5-Iodo-2’-deoxyuridine (IdU ...
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bioRxiv - Cell Biology 2024Quote: In-gel samples were reduced with 5 mM tris(2-carboxyethyl)phosphine (TCEP) (Sigma Aldrich), alkylated with 5 mM N-Ethylmaleimide (NEM) ...
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bioRxiv - Neuroscience 2023Quote: Proliferating cells were labelled with the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) (Sigma; 900584) at a dose of 50 mg/kg body weight in vivo or 1 μM in vitro ...
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bioRxiv - Pathology 2023Quote: ... and then with 5% goat serum and 2% BSA (Sigma-Aldrich, St. Louis, MA, USA). Staining was performed using the VECTASTAIN Elite ABC HRP kit (PK-6200 ...
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bioRxiv - Immunology 2023Quote: Islet MTs were treated with IL-1β (2, 5 or 10 ng/mL, Sigma-Aldrich), IFNγ (10 ...
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bioRxiv - Physiology 2023Quote: ... 15-35 µg protein per sample were denatured with 5 % v/v 2-mercaptoethanol (Sigma) in LDS sample buffer (Thermo ...
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bioRxiv - Systems Biology 2023Quote: ... tissue sections were deparaffinized by washing 2×5 minutes in Neo Clear (Sigma Aldrich, 1.09483.5000), followed by a series of 99% ...
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bioRxiv - Neuroscience 2023Quote: ... uridine and 5-Fluoro-2’-deoxyuridine were purchased from Sigma-Aldrich (St. Louis, MO, USA); D-APV (D-(-)-2-Amino-5-phosphonopentanoic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... hESCs medium with 25 μM 5-chloro-2′-deoxyuridine (CldU; Sigma-Aldrich, St. Louis, MO) was added to the cultures ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with 100 μM of 5-bromo-2-Deoxyuridine (BrdU, Sigma Aldrich B5002) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... After 2 days incubating with 5 μM ROCK Inhibitor (Y-27632, RI, from Merck Millipore), 40 μM TGF-β inhibitor (SB 431524 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich 68957) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich 22790) ...
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bioRxiv - Cell Biology 2024Quote: ... Senescence was induced by treating HeLa cells with 200μM 5-Bromo-2’-deoxyuridine (BrdU, Sigma) freshly prepared in DMSO or exposing the cells to ionizing radiation (8 Gy ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by a second labelling with 200 mM 5-iodo-2-deoxyuridine (IdU, Sigma-Aldrich). For HU treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 and reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich, C4706) and alkylated with 55 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... a mix of 37 mM uridine and 27 mM 5-fluoro-2-deoxyuridine (Sigma Aldrich) was added to the cultures.
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bioRxiv - Cell Biology 2024Quote: ... and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation ...
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bioRxiv - Neuroscience 2024Quote: Proliferating cells were labelled with the thymidine analogue 5-ethynyl-2′-deoxyuridine (EdU) (Sigma; 900584) at a 1 µM concentration that was added to the culture media 1 h before fixation ...
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bioRxiv - Plant Biology 2024Quote: ... 5 mM sodium ascorbate) on ice and filtered through 2 layers of Miracloth (Merck Millipore). Intact chloroplasts were collected from a band at the interface between the 40 % (v/v ...
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Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liverbioRxiv - Immunology 2024Quote: ... Sections were washed 2 times for 5 minutes in 0.5% glacial acetic acid (Sigma-Aldrich). Tissue sections were dehydrated through an ethanol series (70% ...