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Citations for Millipore Sigma :
2551 - 2600 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Immunology 2021Quote: ... Bound protein was eluted in presence of 1M methyl α-D-mannopyranoside (Sigma). The protein was dialyzed against PBS and subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Eluted protein was desalted using PD-10 desalting columns (Sigma-Merck, U.S.A/Germany). Concentration of the desalted protein was estimated using Bradford’s method and the protein was stored at -80 °C until needed.
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bioRxiv - Plant Biology 2021Quote: ... The purified proteins were concentrated using an Amicon Ultra-4 10 K (Millipore).
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bioRxiv - Plant Biology 2021Quote: ... and protein expression was induced by addition of doxycycline 1μg.mL-1 (PHR1145, Sigma). 6 hours after doxycycline addition ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore Sigma).
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bioRxiv - Microbiology 2021Quote: ... followed by transfer of the proteins onto Immobilon FL PVDF membrane (Millipore, Germany). Antibodies against the T3SS components EspA (1:500) ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... the protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). A90C α-synuclein was labelled with 1.5-fold molar excess Alexa Fluor 488 C5 maleimide (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was concentrated using Amicon Ultra-15 centrifugal filter units (Millipore, Germany) to around 8 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were resolved by SDS-PAGE and transferred onto PVDF membranes (Millipore). Membranes were blocked with 2% BSA in TBS tween20 0.1% and incubated in the presence of the primary and then secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were reduced with 10 mM Tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich) for 1 h at room temperature followed by alkylation of cysteine residue side chain thiol groups with iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Biophysics 2021Quote: ... The protein fractions were pooled and concentrated using 3kDa cutoff ultracentrifugal device (Millipore). The protein was analyzed on 12% SDS-PAGE and mass spectrometry ...
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bioRxiv - Biophysics 2020Quote: ... The protein was then concentrated via centrifugal filters (MWCO 10 kDa; Millipore Sigma) and the buffer was exchanged into final buffer by PD-10 desalting column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred to a methanol activated polyvinylidene difluoride (PVDF) membrane (Millipore) in Bis-Tris or Tris-Glycine transfer buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and loading control protein □-Tubulin (Sigma Aldrich ref: ref T9026, diluted 1/20,000). The secondary antibodies used were anti-mouse IgG ...
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bioRxiv - Genomics 2021Quote: ... Dialyzed Tn5 protein was concentrated by using ultracel 30-K column (Millipore, UFC903024) and the quantity of Tn5 was measured by Bradford assay and visualized on NuPAGE Novex 4-12% Bis-Tris gel (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% glycerol with 5000x diluted SYPRO® Orange protein gel stain (Sigma-Aldrich) in total reaction volume 25 μl ...
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bioRxiv - Cell Biology 2021Quote: ... Equal total proteins loads were confirmed by Ponceau red (0.1% Ponceau, Sigma-Aldrich), 5% acetic acid (v/v)] staining of the membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge and flash frozen in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... The protein complexes were eluted with wash buffer containing 10 mM desthiobiotin (Sigma) and treated with TEV protease at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... individual plasmids were transformed into BL21 Rosetta (DE3) protein expression cells (EMD Millipore).
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bioRxiv - Biochemistry 2022Quote: ... the protein was first digested at 16°C with an immobilized pepsin (Sigma) cartridge (2 mm x 2 cm ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride membranes (Millipore) according to the standard protocols ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated by SDS-PAGE and transferred overnight to nitrocellulose membranes (Millipore). Membranes were blocked with 5% bovine serum albumin in tris-buffered saline with 0.05% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... bead-bounded proteins were reduced with 100 mM Dithiothreitol (DTT) (Sigma-Aldrich, 43815) and alkylated using 100 mM iodoacetamide (Applichem ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-heat shock protein 70 (HSP70) (H5147, Sigma-Aldrich, 1:500), rabbit monoclonal anti-Alix (MCA2493 ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Microbiology 2022Quote: Coding sequences for M proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site (5) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Proteins were then precipitated upon addition of 50% trichloroacetic acid (TCA, Sigma-Aldrich) to a final concentration of 5% ...
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bioRxiv - Neuroscience 2022Quote: Total protein from A1 was extracted using RIPA buffer (#R0278, Sigma Aldrich, USA) and protease inhibitor cocktail kit (#78410 ...
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bioRxiv - Cancer Biology 2022Quote: ... required protein amounts resolved by SDS-PAGE and transferred to nitrocellulose membrane (Millipore). Western blot analysis was performed according to standard procedures ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were incubated with 3ul anti-FLAG antibody (Clone M2, Sigma-Aldrich) and 50ul Protein-G magnetic beads (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Misfolded proteins were detected by means of thioflavin-S (Sigma Aldrich; 1:1000) staining ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and proteins were transferred to a polyvinylidene difluoride (PVDF; Millipore, Bedford, MA, USA). Membranes were blocked with bovine serum albumin (1 % ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were digested by addition of 20 μL of proteinase K (Sigma, P6556) at 20 mg/ml to the samples and incubation for 2 hours at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using 10kDa Amicon filter (Merck Millipore, Burlington, MA, USA). The protein concentration in each fraction was estimated using the Bradford reagent (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched with a Roche EDTA protein inhibitor tablet (Sigma-Aldrich) at 1X concentration and the solution was then passed over a CaptureSelect™ IgG-CH1 column (ThermoFischer Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were transferred onto a 0.45 μm methanol-activated PVDF membrane (Immobilon, Millipore) overnight at 4°C at 25V and 45 min at 4°C at 75V the next day ...
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bioRxiv - Microbiology 2022Quote: ... GST-tagged proteins were purified under native conditions on gluthatione-agarose resin (Sigma). The recombinant proteins were then washed 3 times with Buffer A (100mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... and MBP-tagged and associated proteins were eluted in 60 mM maltose (Sigma) for 30 minutes on ice.
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bioRxiv - Cancer Biology 2022Quote: ... filtered through a low protein binding filter unit (PVDF, 0.45 um, Sigma-Aldrich), and stored at 4 degrees C for up to 5 days prior to use ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... Proteins and phosphorylation-sites were transferred to a polyvinylidene difluoride membrane (PVDF, Millipore), where the PVDF membrane was blocked after the transfer (5 min ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were then concentrated using AmiconTM Ultra 15 concentrators (MERCK Millipore) and stored in -80°C.
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 100 µg protein where loaded on a gel to detect endogenous MDGA proteins in Western blots ...
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bioRxiv - Plant Biology 2021Quote: ... 8 μg of purified proteins were incubated with 1 mM DMAPP (Sigma-Aldrich) and 1 mM AMP or ATP for two hours at 30°C in 12.5 mM Tris–HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... The protein bands were detected using 3,3 ́-diaminobenzidine tetrahydrochloride hydrate (DAB) (Sigma, D5637). The blots were incubated in a solution containing 1mg/ml DAB in 50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). The membranes were incubated with appropriate mouse antibodies directed against TLR4 (ab22048 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and protein concentration was measured by Bradford assay (Sigma).