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Citations for Millipore Sigma :
2451 - 2500 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Completeness of protein transfer was determined by Ponceau S (Sigma-Aldrich, cat# P7170) staining for 5-6 min followed by frequent washing with H20 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were immunoprecipitated by incubating cell extracts overnight with either FLAG M2 (Sigma) or GFP (Clonetech ...
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bioRxiv - Cell Biology 2019Quote: ... Total extracted proteins (20 μg) were derivatized according to the manufacturer’s instructions (Millipore). 2-mercaptoethanol was used to reduce samples ...
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bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
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bioRxiv - Cell Biology 2020Quote: Protein loading quantity was controlled using a monoclonal anti-α-tubulin antibody (Sigma). Secondary antibodies were from Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then transferred to Immobilon-P transfer membranes (PVDFs) (IPVH00010; Millipore). The membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies against the following proteins were used: actin (rabbit, Sigma-Aldrich, #A2066), cMyc (mouse ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μl lambda protein phosphatase (800 U) and buffer components as instructed (Sigma) and the reaction was terminated after 30 min at 30 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 300µg of proteins were incubated with 30µl of Streptavidin-Agarose beads (Sigma, CS1638) on a rotating wheel at 4°C for 3hr ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins samples were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore) for Western blot analysis.
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bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was mixed with 30 μL of protein A Sepharose (Sigma P3391) and rotated for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma) and Protein G Mag Sepharose (GE healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... inner-membrane proteins were solubilized using 0.5% (w/v) N-lauryl sarcosine (Sigma) in a buffer containing 20 mM Tris-HCl [pH 7.5] and 150 Mm NaCl for 1.5 h at 4 °C on a roller ...
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bioRxiv - Immunology 2021Quote: ... and filtering through 0.65 µm low protein binding filter plates (Millipore, Burlington, USA).
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bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
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bioRxiv - Developmental Biology 2021Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore, Burlington, MA) in transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Priming was done with 50μg of protein in complete Freund’s adjuvant (Sigma, India) per mouse ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins associated with 3xFLAG-tagged MBD3 were purified using anti-FLAG sepharose (Sigma) and processed for mass spectrometry as described (Smits et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... 50μL of pre washed Magna ChIP protein A+G magnetic beads (Millipore Sigma) were then added to the samples and rotated at 4°C for 2 hours to elute the chromatin:antibody complexes ...
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bioRxiv - Immunology 2022Quote: ... were coated with 20μg/ml OVA protein (Sigma Aldrich, St Louis, MO, USA) or 4μg/ml CII (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
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bioRxiv - Biophysics 2022Quote: ... gels were stained for 1 h using ReadyBlueTM Protein Gel Stain (Sigma-Aldrich) and destained with ddH2O overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted by boiling with Sample Buffer Laemli 2X (Sigma-Aldrich) for 5 min at 95°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The captured DNA-RNA hybrids were enriched by protein A-agarose beads (Millipore). The purified DNA was used to construct a library with the NEXTflex Rapid DNA-seq Kit (PerkinElmer).
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bioRxiv - Molecular Biology 2020Quote: ... the protein is transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore Corp., MA, USA) at 20 V ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluates were incubated with 40 μl of S-protein-agarose beads (Millipore) for 2 hr at 4 °C and then washed three times with NETN buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on 6% polyacrylamide gel and transferred on PVDF membrane (Millipore) using semi-dry blotting ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG-tagged fusion proteins were immunoprecipitated using Anti-FLAG M2 magnetic beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing protein were concentrated in 50-kDa-cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at -80 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Eluted proteins were buffer exchanged and concentrated with PBS using Amicon tubes (Millipore). The proteins were further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 125 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatants were precleared with 20 μL of protein A/G magnetic beads (Millipore) at 4°C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... the protein content and purity were evaluated with SDS-PAGE and InstantBlue (Sigma) gel staining (Extended Data Fig ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then visualized with ProteoSilver Plus Silver Stain Kit (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore) by ultracentrifugation and purity was controlled by SDS-PAGE.
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bioRxiv - Cancer Biology 2019Quote: ... The respective protein bands were visualized using the immunobilion chemiluminescence system (Millipore, MA). The densitometry analysis of immunoblotting images was performed using Quantity One Software (Bio-rad) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein levels were normalized to β-actin (1:5000 dilution, A5060, Sigma Aldrich). Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were precleared by a 30 minute-incubation with Protein G agarose (Sigma), and then incubated for 2 hours at 4°C with Protein G agarose and 5 µg of indicated antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... and the protein concentration was assessed using a BCA kit (Sigma-Aldrich, USA). Samples were diluted to equal concentrations and separated using the Novex Native PAGE Bis–Tris Gel system (4–16% ...
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bioRxiv - Immunology 2019Quote: ... Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore, Watford, UK) visualized on Hyperfilm™ (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Immunology 2019Quote: ... SDS-PAGE separated proteins were transferred onto Immobilon-FL PVDF membranes (EMD Millipore) at 100V for 1h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore cat#WBKLS0500) [7].
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bioRxiv - Biophysics 2020Quote: ... and 10 μL of 5 mg∙mL−1 of protein A (Sigma-Aldrich), were added to 1 mL of 10 mg∙mL−1 of N-(3-dimethylaminopropyl)-N’-ethylcabodiimidie hydrochloride (EDC ...
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bioRxiv - Immunology 2020Quote: Protein lysates were subjected to SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked and probed with one of the following antibodies ...
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bioRxiv - Bioengineering 2019Quote: ... Antibodies were purified from medium using protein G gravitrap (Sigma, 28-9852-55) according to manufacturer protocol ...
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bioRxiv - Microbiology 2019Quote: ... proteins precipitated on the beads were eluted using Laemmli’s buffer containing dithiothreitol (Sigma) boiled 5 min at 95 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... protein synthesis was inhibited by treating cells with 350 µM cycloheximide (Sigma Aldrich).