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Citations for Millipore Sigma :
2751 - 2800 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.2 μm PVDF membrane (#ISEQ85R; Sigma-Aldrich/Merck) by electroblotting the separated proteins for 1 h at 100 V ...
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bioRxiv - Developmental Biology 2022Quote: ... A GST-Drn fusion protein was produced in Origami B (DE3) cells (Novagen) and purified with a glutathione-Sepharose 4B column ...
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bioRxiv - Plant Biology 2022Quote: ... Soluble protein was purified with 200 μl of Glutathione agarose beads slurry (Sigma), gently shaking for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were transferred to an Immobilon®-P PVDF membrane (Merck Millipore Ltd.) in a Biometra Fastblot B34 chamber at 1 mA/cm2 membrane for 1.5 h in transfer buffer (20 mM CAPSO pH 11.2 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein levels were assessed using immunoblots with HA-HRP (Sigma, Catalog number 12013819001) or FLAG-HRP antibodies (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were collected from each well and analysed by silver staining (Sigma) and Western blot using monoclonal anti poly histidine alkaline phosphatase antibody (1:20,000 dilution) ...
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bioRxiv - Microbiology 2022Quote: ... was added to 75 μl of prewashed protein A sepharose beads (Sigma-Aldrich) together with 700 μl lysis buffer (20 mM Tris/HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF; Millipore) membranes ...
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bioRxiv - Microbiology 2022Quote: ... Pure Cas protein was finally concentrated in Amicon Ultra centrifugal filter (Merck-Millipore).
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bioRxiv - Microbiology 2022Quote: ... Digested glycans were cleaned using a 96-well PVDF protein-binding membrane (Millipore) attached to a vacuum manifold ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were transferred to low protein binding tubes (Sigma-Aldrich, Cat# Z666513-100EA) and stored at −80°C
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bioRxiv - Neuroscience 2022Quote: ... and the separated proteins were then transferred to polyvinylidene difluoride membranes (Millipore, USA). These membranes were blocked in 5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany). Unspecific binding on the PVDF membrane was blocked by incubation with the blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resuspended in 50 mM triethylammonium bicarbonate (TEAB, Sigma cat. no. T7408), reduced for 1 hour at 55°C using 10 mM tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Neuroscience 2023Quote: ... Residual protein fragments were precipitated with trichloroacetic acid (Sigma-Aldrich, cat. no. T6399). The supernatant ...
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bioRxiv - Microbiology 2022Quote: ... YifLStrep protein was eluted with wash buffer containing 2.5 mM d-Desthiobiotin (Sigma).
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein detection using either Immobilon Western HRP Substrate (WBKLS0500, Geyer/Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse anti-glial fibrillary acidic protein (GFAP; 1:500, #G3893; Sigma-Aldrich). The next day sections were washed 3 times with the blocking buffer (15 min each ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of neuronal marker protein NeuN (anti-NeuN, 1:200, MAB377, Millipore) was determined to identify neuronal tdTomato expression.
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using a Quantipro BCA assay kit (Sigma-Aldrich, USA). Protein lysates were prepared in a mixture of RIPA and Lamelli Buffer to produce a final protein concentration of 5 ug/ul ...
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bioRxiv - Molecular Biology 2022Quote: ... The resolved protein samples were transferred onto Immobilon-P PVDF Membrane (Millipore Sigma), and the blots incubated in the presence of primary antibodies (provided in table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal loading of protein samples was confirmed via Ponceau S staining (Sigma-Aldrich) for 4 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge at 4 °C ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Proteins were analyzed by SDS-PAGE and transferred onto PVDF membrane (Merck Millipore). The membranes were washed with PBS 1x containing 0.1% Tween-20 (PBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted protein fractions were pooled and concentrated using Amicon 30K Centrifugal filters (Millipore). The concentrated ...
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bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing protein were concentrated using Amicon Ultracel-10K/ Ultracel-30K (Millipore) centrifuge filters and subsequently applied to size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2022Quote: ... 10 μg protein was combined with 1 μL 10% Triton X-100 (Sigma) and brought to a volume of 33 μL in Vance buffer (225 mM D-mannitol ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mM purified protein was mixed with 25% ammonium persulfate (APS) (Sigma-Aldrich) and 6.67 mM [Ru(II)(bpy)3]2+ (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... The expressed GST-fusion protein was absorbed to glutathione-Sepharose 4B (Sigma-Aldrich) followed by extensive washing with phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... Fractions containing the protein of interest were concentrated (Amicon Ultracel 30 K, Millipore) and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rabbit anti-zinc transporter 3 (ZnT3 ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was concentrated in a centrifugal filter (Amicon Ultra-15, 10K, Millipore) and washed twice with 15 ml of PBS to remove the imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was pre-cleared with Protein A agarose beads (Millipore, Sigma), and 700 μg of the pre-cleared lysate was incubated with 2 μg of either anti-CEP41 ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). The PVDF membranes were then incubated with a buffer of 5% nonfat dry milk for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was pre-cleared with Protein A agarose beads (Millipore, Sigma), and 700 μg of the pre-cleared lysate was incubated with 2 μg of either anti-CEP41 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins of interest were detected with anti-FLAG antibody (Sigma F1805, 1:1000) and anti-PCNA antibody (Santa Cruz sc-8349 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with addition of 1 µL of 500 mM dithiothreitol (Sigma) at 25°C for 1 h and alkylated with 3 µL of 500 mM iodoacetamide for 1 h at room temperature in the dark and quenched with 2 µL of dithiothreitol for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were incubated with 0.2mM methoxypolyethyleneglycol maleimide (NEM-PEG, 5kDa; Sigma-Aldrich) for 1 minute on ice ...
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bioRxiv - Biophysics 2024Quote: ... The dialyzed sample was filtered with a low protein binding filter (Millipore Sigma) and run through a SourceQ15 anion exchange column (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
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bioRxiv - Developmental Biology 2023Quote: ... The lysates were pre-cleared using Protein A agarose beads (11134515001, Sigma-Aldrich) at 4 ℃ for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich), and equal amounts of protein were analyzed by western blot.
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bioRxiv - Cell Biology 2024Quote: ... The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich) and centrifuged (12 000 g ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were electrophoretically transferred overnight on to PVDF membrane (03010040001, Roche, Sigma-Aldrich). The membrane was blocked in 5% non-fat dry milk in TBS with 0.05% Tween 20 (TBST ...
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bioRxiv - Microbiology 2024Quote: ... External mass calibration was performed thanks to SpheriCal Aqua Protein Low kit (Sigma).
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bioRxiv - Immunology 2024Quote: ... Eluted proteins were buffer exchanged using 50 kDa Amicon centrifugal units (EMD Millipore), resuspended in phosphate-buffered saline (PBS) ...