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Citations for Millipore Sigma :
2351 - 2400 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred on a MeOH-activated polyvinylidene fluoride (PVDF) membrane (Millipore) by semidry blotting for 1 h at 10V ...
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bioRxiv - Genetics 2022Quote: ... Eluted proteins were loaded on the centrifugal filter unit (Millipore-Sigma, UFC8030) and centrifuged at 3,500 g 4°C for 20 minutes to eliminate any remaining maltose from the elute ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentration was quantified by Coomassie staining using BSA standards (Sigma P0914).
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bioRxiv - Genomics 2023Quote: ... protein stability was assessed by adding 5 mg/ml cycloheximide (Sigma-Aldrich) to the cell growth medium for the indicated times prior to harvest ...
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bioRxiv - Genetics 2022Quote: ... Eluted proteins were loaded on the centrifugal filter unit (Millipore-Sigma, UFC8030) and centrifuged at 3,500 g 4°C for 20 minutes to eliminate any remaining maltose from the elute ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 μg/mL doxycycline hyclate (Sigma Aldrich), and the culture was placed in a shaking incubator maintaining 37°C and a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was transferred to a low background fluorescence PVDF membrane (Millipore Sigma) and the membrane was blocked in 5% nonfat milk in 1x TBST ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred on an EtOH-activated polyvinylidene fluoride (PVDF) membrane (Millipore) by wet blotting overnight at 40 mA and 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were directly labeled using alkaline phosphatase-coupled streptavidin (Sigma-Aldrich). The blots were developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2022Quote: ... The protein bands were visualized by an ECL detection system (EMD Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... Cell lysates were incubated with Protein G sepharose beads (Sigma-Aldrich P3296) with anti-RpoC antibody (Biolegend clone NT73 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then filtered through a 10kDa protein filter (MRCPRT010, Sigma Aldrich) via centrifugation at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The dialyzed protein was concentrated using a 30 kDa MWCO concentrator (Millipore) and was further purified by size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
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bioRxiv - Cell Biology 2023Quote: ... protein inhibitor cocktail (1:100) and Benzonase nuclease (Novagen, 70746, 1:100) in PBS ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich). Prior to their use ...
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bioRxiv - Systems Biology 2023Quote: ... We then transferred proteins to a low background PVDF membrane (Millipore, IPFL00010) for 15h at 15V using the TRIS-Glycine buffer (48 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... proteins were electrotransferred to a polyvinylidene difluoride membrane (Millipore, Burlington, MA, USA) using a semi-dry transfer blotter (Atto) ...
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bioRxiv - Cell Biology 2023Quote: ... Bound proteins were eluted in Flag peptide (Sigma, F4799, 150 ng/μl) for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: Pure proteins were concentrated using 10 kDa Amicon Ultra membrane concentrators (Millipore), flash-frozen in liquid nitrogen and stored at −80℃.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using the Bicinchoninic acid assay (BCA, Sigma-Aldrich). Proteins were then denaturated with Laemmli Buffer (Tris pH 6.8 (200mM) ...
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bioRxiv - Neuroscience 2023Quote: ... transferrin and transthyretin) using the ProteoPrep 20 plasma protein immunodepletion kit (Sigma), by performing two immunodepletion cycles ...
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bioRxiv - Biochemistry 2023Quote: ... Both the proteins were concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... total protein was visualized using PonceauS staining (Sigma-Aldrich, St. Louis, MO), blocked using 5% non-fat milk powder in tris-buffered saline with tween (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (1:1000, Millipore Sigma, #MAB3402), rat monoclonal anti-CD45 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg protein per sample were reduced with 10 mM DTT (Sigma) for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, USA), which was then blocked with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated with 1:4 trichloroacetic acid solution (TCA, Sigma Aldrich) and washed with acetone ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg protein per sample was reduced with 3 mM TCEP (Sigma) and alkylated with 10 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and the proteins transferred onto polyvinylidene fluoride membrane (Immobilion-P, Millipore, USA) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich, #D141-100MG). Mitochondria were incubated on ice for 20 minutes and then centrifuged at 20,000 x g for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO). Chemiluminescence was measured in a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Purified protein was then concentrated to 40mg/mL by centrifugal concentration (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... Polyclonal antibodies from antiserum were purified using Protein A Sepharose (Sigma, USA) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by immunoblotting using anti-Glutathione-S-Transferase (Sigma-Aldrich), anti-polyHistidine-Peroxidase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained using ReadyBlue protein gel stain (Sigma-Aldrich, RSB-1L) followed by de-staining with water ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated using Amicon Ultra-4 10 kDa MWCO (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... They were incubated with recombinant ULK1 proteins (Sigma, #SRP5096-10UG, Darmstadt, Germany) in the kinase buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... Proteins were visualized using peroxidase-coupled secondary antibodies (rabbit-POD, Sigma-Aldrich, A6154 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 h on ice before mixing with protein G-Sepharose (Millipore). The antibody complex was washed three times in lysis buffer and once in Tris-buffered saline (TBS) ...
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bioRxiv - Immunology 2023Quote: Dry protein pellets were dissolved in 100mM TrisHCL (pH 8.5, Sigma T1503) to determine the protein concentration using the Bradford protein assay (Thermo Scientific 23200) ...
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bioRxiv - Molecular Biology 2023Quote: ... protein synthesis was inhibited by blocking translation step using cycloheximide (Sigma, #C7698) and MMP activity was inhibited by ilomastat (GM6001) ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein was extracted in RIPA buffer containing protease inhibitor mixture (Sigma) and phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transferred proteins were stained in Ponceau S (0.1% Ponceau S (Sigma-Aldrich), 5% (v/v ...