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Citations for Millipore Sigma :
2701 - 2750 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... antimicrotubule-associated protein 2 (MAP2) Alexa Fluor 647 (1:500, NB120-11267AF647; Millipore). Cell nuclei were stained by using Hoescht 33342 (2 μg/mL ...
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bioRxiv - Synthetic Biology 2021Quote: ... The eluted proteins were concentrated using Amicon® Ultra Centrifugal Filters (Millipore Sigma). The concentrated proteins were dialyzed into SUMO-SIM protein buffer (150 mM KCl ...
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bioRxiv - Biophysics 2020Quote: ... Purified labelled protein was concentrated using a 50kDa MWCO centrifugal filter (EMD Millipore) and stored at −80°C in buffer containing 10mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: ... The resulting protein samples were individually incubated with anti-GFP agarose beads (Sigma) for 4 h at 4°C followed by five rinses in an ice-cold PBS buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were reduced and alkylated for 1 h with 15 mM TCEP (Sigma) and 30 mM CAA (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: The immunoprecipitated protein eluates were treated with 0.1 U of benzonase (Sigma, E8263) for 30 min at 37 °C and further diluted with the same volume of 100 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins blotted onto nitrocelullose membranes were probed with anti-HA H3663 (Sigma-Aldrich), MyD88 4D6 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: All proteins were expressed and purified from E.coli Rosetta (DE3) Competent Cells (Novagen). Details of individual constructs and their expression plasmids are detailed in the plasmids section ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were then alkylated by the addition of 10 mM iodoacetamide (Sigma-Aldrich) for 45 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated using an Amicon Ultra 10 K device (Merck Millipore), loaded on a size exclusion column [ENrich SEC 650 10 x 300 Column (Bio-Rad)] ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced by the addition of tetracycline (1 μg/ml; Sigma) for desired time (determined during time course).
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bioRxiv - Biophysics 2021Quote: ... The protein eluent was concentrated by a 100-kDa cut-off Centricon (Millipore) and further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then eluted with 5 folds volume of Glutathione solution (Sigma-Aldrich), and were condensed using 10kD protein concentrators (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge and flash frozen in liquid nitrogen.
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bioRxiv - Immunology 2020Quote: ... Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich). For the endogenous interaction assay ...
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bioRxiv - Microbiology 2020Quote: ... proteins were fractionated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore), and probed with the indicated antibodies.
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bioRxiv - Microbiology 2020Quote: ... cut protein bands were first DTT reduced (Sigma-Aldrich, St. Louis, MO, USA) to be subsequently treated with Iodacetamide for protein alkylation (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... washed in PBS and processed using ProteoExtract Native Membrane Protein Extraction Kit (Sigma). Soluble cytoplasmic ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysates were incubated with either anti c-MYC agarose beads (Sigma A7470), anti c-MYC magnetic beads (Santa Cruz 500772) ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with anti-FLAG M2-HRP (1:3000 dilution, Sigma #A8592).
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bioRxiv - Immunology 2020Quote: ... followed by binding of protein A/G magnetic beads (Merck Millipore cat. LSKMAGAG02) for 4 h at 4℃ ...
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bioRxiv - Microbiology 2021Quote: ... The protein bands were visualised using a ProteoSilver Silver Stain kit (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... Protein loading was determined using an anti-β-actin (AC-74, Sigma-Aldrich) antibody ...
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bioRxiv - Cell Biology 2020Quote: ... 1mg protein lysates were incubated with 30ul Flag antibody-conjugated agarose beads (Sigma) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were detected by enhanced chemiluminescence (Classico substrate, Immobilon-Millipore, USA, #WBCUC0500) and visualized by G ...
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bioRxiv - Immunology 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane (Amersham™ Protran®, Sigma-Aldrich). For blot development and detection ...
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bioRxiv - Genomics 2021Quote: ... and rabbit anti-Protein A antibodies (Sigma, Cat No. P3775, dilution 1:1000) were added ...
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bioRxiv - Cell Biology 2021Quote: ... Protein loading was controlled by Vinculin (VCL) antibody (1:5000, V3131, Sigma-Aldrich). Horseradish peroxidase conjugated goat anti-mouse IgG antibody (1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein expression was induced using 200µM isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma). Bacterial cells were pelleted and resuspended in 20ml Buffer A (for His-tagged ...
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bioRxiv - Cancer Biology 2019Quote: ... To generate free aldehydes from oxidize sugar groups in matrix protein (fibronectin, Sigma), sodium periodate (∼3.5mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Protein quantification of the cleared lysates was performed by Bradford colorimetric assay (Sigma) using a BSA standard curve ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used to detect proteins: anti-tubulin (Sigma-Aldrich T6199), anti-IE1 (1B12 ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were separated in SDS-PAGE and transferred to PVDF membrane (Millipore) and subsequently probed with respective antibodies including anti-RAD51 (8875S ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-mouse GAPDH 0.2 μg/mL (Millipore Sigma, G8795; protein loading control). IRDye®-800CW goat anti-rabbit and −680RD goat anti-mouse IgG secondary antibodies at a 0.1 μg/mL concentration (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was then concentrated in an Amicon Ultra centrifugal filter (Millipore Sigma) to less than 2 mL total volume ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Cat. #PVH00010 Millipore, Bedford, MA) as described (Popova et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then transferred to 0.45 µm pore PVDF (catalog number IPVH00010, Millipore) at 25 to 35 V for 1 hour and incubated with appropriate antibodies overnight at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... RNA-YTHDC1-antibody complexes were pulled down using Dynabeads Protein A/G (Millipore) and washed 3 times with NETN100 buffers (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Protein alkylation reactions were then carried out with 50 mM Iodoacetamide (IAA) (Sigma) at room temperature for 30 minutes in the dark ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was filtered through a 0.22 μm centrifugal filter (Merck Millipore) prior to loading onto the gel filtration column ...
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bioRxiv - Biochemistry 2022Quote: ... RAF protein was then eluted with 200 μg/mL 3xFLAG peptide (Sigma-Aldrich) in TN buffer ...
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bioRxiv - Cell Biology 2022Quote: Protein concentration was calculated from either Coomassie Brilliant Blue G (Sigma-Aldrich; B0770) staining of SDS-PAGE-separated purified proteins and Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... 200 ug protein was loaded onto a Microcon Centrifugal Filters (Millipore, Cat: 42407). Filter aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2022Quote: Total protein was extracted by lysing the cells with RIPA Buffer (Sigma-Aldrich) containing phosphatase and protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 350 µg total protein concentrated with 3-kDa cut-off centrifugal filter (Millipore) from serum-free medium was subjected to SDS-PAGE with multiple band excision and in-gel digestion ...
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bioRxiv - Cell Biology 2022Quote: For analysis of oxidized protein abundance using the Oxiblot assay kit (Millipore, S7150), lysates were prepared in RIPA lysis buffer supplemented with 50 μM DTT ...
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bioRxiv - Cell Biology 2022Quote: Chemical cross-linking of proteins was performed with dithio-bismaleimidoethane (DTME) (Sigma-Aldrich) dissolved in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...