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Citations for Millipore Sigma :
2501 - 2550 of 2566 citations for Recombinant Rat Crp His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Rabbit anti-TgTom40 (van Dooren et al., 2016, 1:2000 dilution) and rat anti-HA high affinity (Sigma, 11867431001, 1:200 dilution) primary antibodies were used ...
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bioRxiv - Pathology 2024Quote: ... The diabetic rats in the DM+GR group were given a standard diet and were also given glyburide (Sigma-Aldrich; Merk KGaA) at 4 mg/kg in 0.5 ml of 0.5% Tween 80 solution as a supplement ...
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bioRxiv - Immunology 2023Quote: ... and subsequently incubated with anti-CD16/CD32 (eBioscience, Cat #14-0161-86, clone 93) and 10% rat-IgG1 (Sigma-Aldrich, Cat #I8015) in PBS containing 0.1% BSA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad], Secondary anti-Rat IgG, [ThermoFisher], and anti-NeuN [Millipore Sigma] ...
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bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... FGF21 was measured by using a commercially available enzyme-linked immunosorbent assay kit (rat/mouse FGF21 enzyme-linked immunosorbent assay kit; Millipore, Billerica, MA), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected and serum insulin levels were determined in triplicate using a rat/mouse insulin Enzyme-Linked Sorbent Assay (ELISA) kit from Millipore (EZRMI-13K) according to the instructions of the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Microbiology 2024Quote: ... incubator with daily medium changes 96-well plates (Falcon: 353219) precoated with Type 1 collagen solution from rat tail (Sigma Aldrich: C3867).
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bioRxiv - Immunology 2024Quote: ... we coated plates with 1 µg/ml of recombinant NP119 and used a goat anti-mouse IgG-HRP (ψ-chain specific) as secondary antibody (Sigma, A3673-1ML; diluted 1:1000). To measure anti-drug antibody responses directed against the AAV-delivered YTS169-IgG2a we coated plates with 1 µg/ml of YTS169-IgG2a or YTS191-IgG2a and used a goat anti-mouse IgG1-HRP (Jackson ...
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bioRxiv - Cell Biology 2020Quote: ... dark agouti rats via a single intraperitoneal injection of streptozotocin (STZ, 55 mg/kg, in trisodium citrate buffer, pH 4.5, Sigma-Aldrich Co, MO, USA), with control animals receiving an equivalent volume of vehicle ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were incubated overnight at 4°C in primary antibody diluted in 5% BSA/PBS using the following concentrations: 1:500 rabbit-α- PfACP48 (kind gift from S. Prigge) and 1:100 rat-α-HA 3F10 (Sigma 11867423001). Coverslips were washed 3 times in PBS ...
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bioRxiv - Neuroscience 2021Quote: Sprague Dawley male rats received four ip injections of 4 mg/kg methamphetamine hydrocholoride (dissolved in 0.9% saline Sigma, St Louis, MO, USA) every two hours for 8 hours with rectal probe body temperature measurements every hour ...
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bioRxiv - Neuroscience 2020Quote: ... Rat pups received oral administration of fluoxetine (10 mg/kg; kind gift from IPCA Laboratories) or vehicle (5% sucrose, Sigma-Aldrich, Cat no.) once daily through a micropipette (0.5-10 μL ...
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bioRxiv - Physiology 2021Quote: ... Cell pellets were resuspended in growth medium and plated in tissue culture dishes coated with type I rat tail collagen (Sigma, St. Louis, MO). Cultures were expanded and frozen at passage 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The tube was kept at 37 °C for 10 min before a 13 μL aliquot of the Sprague-Dawley rat liver microsome (Sigma-Aldrich, No. M9066) was added ...
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bioRxiv - Bioengineering 2020Quote: The effect of HMGB1 on inducing the migration of rat BMSCs were examined using a Trans-well plate (Millipo Sigma, St Louise, USA). The rat BMSCs in DMEM at passage 1 were seeded into the upper layer of insert well of the trans-well plate ...
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bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
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bioRxiv - Neuroscience 2021Quote: ... They were then incubated with secondary antibody (goat anti-mouse Alexa 488 or 555, goat anti-rabbit Alexa 488 or 555, and goat anti-rat Alexa 555, Sigma Aldrich, 1:500) and mounted with Antifade ...
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bioRxiv - Synthetic Biology 2022Quote: Male and female rats were immunized in the flanks with 200 μl of a 1:1 emulsion of ovalbumin (Sigma, St. Louis, MO) in complete Freund’s adjuvant (Difco/Becton Dickinson ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-MBP (Abcam ab7349, 1:100 for tissue, 1:400 for cultured cells; knockout-validated35, rabbit anti-Olig2 (Sigma AB9610, 1:1000), mouse anti-CC1 (Oncogene OP80 ...
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bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
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bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
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bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence was performed according to standard procedures with antibodies against the N-terminus of the laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), the laminin α1 chain (rat monoclonal ...
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bioRxiv - Immunology 2024Quote: ... pH 7.6) + 0.05% v/v Triton-X100 and 10% donkey serum and subsequently incubated with rat-MLKL (Millipore MABC604 clone 3H1 1:1000) overnight ...
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bioRxiv - Genetics 2023Quote: ... Slides were incubated with goat anti-mouse collagen α1(XI) antibody at 1:500 dilution and mouse anti-rat PAX1(Cat #MABE1115M, Sigma-Aldrich, MO, USA), in PBS + 0.1% Triton + 1% normal goat serum at 4L°C overnight ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Neuroscience 2023Quote: ... the blot was probed for HA-epitope (rabbit anti-HA, Cell Signaling #3724, 1:1000; or rat anti-HA, Sigma #11867423001, 1:1000), or PSD95 (mouse anti-PSD95 ...
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bioRxiv - Genomics 2023Quote: ... Rats received a single oral gavage administration of freshly prepared 40 mg/kg N-ethyl-N-nitrosourea (ENU) (Sigma Aldrich, St. Louis, MO) dissolved in PBS (Nova-Tech ...
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bioRxiv - Neuroscience 2023Quote: Western blot analysis of MBP was performed on protein lysates from the anterior cerebrum at P22 as previously described [32] using rat anti-MBP (Millipore MAB386 1:500) and anti-β-actin (Sigma-Aldrich AC-74 ...
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bioRxiv - Biophysics 2024Quote: ... measurements of the identified ChRs were expressed in a mouse neuroblastoma and rat neuron hybrid cell line (ND7/23 cells, ECACC 92090903, Sigma-Aldrich, Munich, Germany). Cells were cultured as previously described (Grimm et al. ...
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bioRxiv - Physiology 2020Quote: ... pregnant rats were randomly assigned to be intraperitoneally injected with DHT (1.66 mg/kg/day, suspended in sesame oil, Sigma-Aldrich, St. Louis, MO, USA) and/or human recombinant INS (6.0 IU/day ...
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bioRxiv - Bioengineering 2021Quote: ... using a Milliplex Rat Gut Hormone Panel 96-well plate assay as described by the manufacturer (#RMHMAG-84K-06; EMD Millipore Corporation, Billerica, MA). Data was collected with MAGPIX with xPONENT software (Luminex ...
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bioRxiv - Neuroscience 2021Quote: ... corresponding to the external Ig-like domain) diluted in sterile PBS and emulsified in an equal volume of incomplete Freund’s adjuvant (IFA, Sigma; total volume 100µl per rat). IFA control rats were immunised with PBS emulsified in an equal volume of IFA ...
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bioRxiv - Neuroscience 2020Quote: ... Cells (primary culture of rat ENS) and tissues from mice (hippocampus, ileum, proximal and distal colon) were lysed in RIPA lysis buffer (Merck Millipore, Fontenay sous Bois, France) containing phosphatase inhibitor cocktail II (Roche ...
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bioRxiv - Microbiology 2019Quote: ... 100 µl of a THP-1 cell suspension (106 cells/mL) in 20 mg/mL collagen from rat tail type I (Sigma Aldrich, St. Louis, USA) were spread in the basolateral surface of inverted inserts ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST before incubating overnight at 4°C in primary antibody solution (4% NGS in TBST) with rat anti-GFAP (Millipore Cat# 345860-100UG, RRID:AB_211868), rabbit anti-GFAP (1:2000 ...
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bioRxiv - Physiology 2020Quote: ... The hydrolyzed TMOS (100 μl) was added to 900 μl of 10 μM of pooled siRNA against rat FL2 (siRNA from Sigma-Aldrich (St Louis, MO): SASI_Rn02 00314854 ...
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bioRxiv - Biochemistry 2021Quote: ... or with anti-GFP primary antibody (1:10000; CHROMTEK 3H9-100 Lot#60706001AB) and then anti-rat-IgG-HRP secondary antibody (1:10000; SIGMA A9037-1ML Lot#SLCF6775). Ponceau staining was used as a loading control.
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bioRxiv - Physiology 2021Quote: ... the experimental rats (body mass 171 to 203 g) were injected subcutaneously with 60 mg monocrotaline in saline/kg body mass (Sigma-Aldrich, St Louis, MO) to induce pulmonary hypertension ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used in this study for immunoblotting were: rat anti-mouse MLKL (WEHI clone 5A6; produced in-house and soon available from Millipore as MABC1634; 1:2000)49 ...
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bioRxiv - Bioengineering 2022Quote: ... As previously described,29 male Sprague Dawley (SD) rats (200-250g) were injected with monocrotaline (MCT) (60 mg/kg, Sigma Aldrich, Oakville, ON, Canada) by intra peritoneal (i.p. ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were loaded on an 8% Tris/glycine gel and subjected to Western blot analysis using different primary antibodies (anti-c-myc (A-14, sc-769, Santa Cruz, Nunningen, Switzerland and HA antibody, rat, 3F10, Sigma-Aldrich, Munich, Germany).
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bioRxiv - Neuroscience 2022Quote: Primary rat glial cells (astrocytes and microglia) and neuroblastoma cells were fixed for 15 min in 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) and washed with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen samples were sent to Eve Technologies and analyzed using the Rat Cytokine/Chemokine Array 27-Plex (BioPlex 200 Cytokine Array Assay Kit Source, Millipore Milliplex; Calgary, AB, Canada).
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bioRxiv - Developmental Biology 2022Quote: ... Four to five-week-old donor rats were superovulated by intraperitoneal injection of pregnant mare serum gonadotropin (30 units, G4877, Sigma-Aldrich, St. Louis, MO), followed by an intraperitoneal injection of human chorionic gonadotropin (30 units ...
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bioRxiv - Immunology 2022Quote: ... For surface staining cells were incubated with mastermix prepared in Fc-block (1%FCS/PBS with 0.1% rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Plasma and dorsal hippocampal CA1 cytokines were assessed using a MILLIPLEX® Rat Cytokine/Chemokine Magnetic Bead Panel (Millipore Sigma Cat. No. RECYTMAG-65K) customized for detection of pro-inflammatory (TNF-α ...
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bioRxiv - Microbiology 2023Quote: ... All well plates and culture flasks were coated prior to seeding with 7 µg/mL rat tail tendon collagen I (Sigma-Aldrich, St. Louis, MO) and rinsed with PBS 3 times ...
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bioRxiv - Neuroscience 2023Quote: All plates required for cell culture were coated with 100µg/mL with collagen rat tail type I (20nM acetic acid/distilled water) overnight before seeded with cells (Sigma-Aldrich; UK, ThermoFisher Scientific; USA). Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco ...