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Citations for Millipore Sigma :
2251 - 2300 of 2566 citations for Recombinant Rat Crp His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant protein from the cell lysate was purified using Glutathione Sepharose® 4B GST affinity columns (Millpore Sigma, GE17-0756-05). The purified PCM1-GST fusion protein was analyzed on SDS-PAGE stained with Coomassie Blue (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Anti-His (SCBT, #sc-8036; used in 1:2000), anti-GST (SCBT, #459; used in 1:5000) and anti-Flag (Sigma-Aldrich, #F7425;used in 1:2000). Peroxidase conjugated anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto a nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2019Quote: ... The proteins of the eluate were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2020Quote: ... rat anti-mouse RIPK3 (clone 8G7; produced in-house5 and available from Millipore as MABC1595; 1:2000/1:400), rat anti-mouse RIPK3 (clone 1H12 ...
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bioRxiv - Microbiology 2021Quote: ... Horseradish peroxidase-conjugated goat anti-chicken IgY and horseradish peroxidase-conjugated rabbit anti-rat IgG (Sigma, St. Louis, MO) were used for western blotting at a dilution of 1:80,000 ...
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bioRxiv - Neuroscience 2020Quote: ... and multiunit activity (MUA) were performed from head-fixed rats under light urethane anesthesia (0.5 g/kg body weight, i.p.; Sigma-Aldrich) using custom made one-shank 1632-channel electrodes (0.5-3 M_Ω Silicon Michigan probes ...
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bioRxiv - Neuroscience 2021Quote: ... About 0.5∼1 hour before sleep onset, rats received intraperitoneal/IP injections of vehicle (i.e., saline as the “sham”) or L655,708 (Sigma-Aldrich; 5 mg/kg ...
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bioRxiv - Immunology 2022Quote: ... The affinity Sepharose resin was prepared by coupling purified rat anti-mouse YN1.1 antibody with CNBr-activated Sepharose 4B (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... PV (mouse anti-Parvalbumin monoclonal antibody, 1:3000, Swant) and SOM (rat-anti Somatostatin monoclonal antibody 1:250, Millipore). The following corresponding secondary antibodies were used ...
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bioRxiv - Cell Biology 2019Quote: ... blotted and immunodetected with a rat anti-CLASP2 or mouse anti-α-tubulin antibodies (clone B-512, Sigma Aldrich). For analysis of recombinant CLASP2 C-term ...
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bioRxiv - Molecular Biology 2019Quote: ... male Wistar rats (n=5-6/group) were deeply anesthetized with sodium phenobarbital (Sigma-Aldrich; 50 mg/kg i.p.), and then positioned in a stereotaxic frame (TrentWells ...
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bioRxiv - Neuroscience 2021Quote: All rats were anesthetized with chloral hydrate and perfused transcardially with phosphate buffered saline (PBS, pH 7.4, Sigma Aldrich) followed by 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2020Quote: ... Primary rat hippocampal neurons were dissociated from E18 Sprague-Dawley rat embryos (Janvier labs) and plated on the SN membranes previously treated with 1 mg.ml-1 poly-lysine (Sigma) in a 0.1 M borate buffer pH 8.5 ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: Mice aged 6 months or naked mole-rats aged 2-4 years were given intraperitoneal (i.p.) injections with 150mg/kg 5-Fluorouracil (5-FU; Sigma) from a 50mg/ml stock in DMSO diluted with sterile 0.9% sodium chloride solution (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 65% v/v acidified type I rat tail collagen (2.035mg/mL, First link, UK) and 10% v/v of 10X minimal essential medium (MEM, Sigma) were mixed and neutralised ...
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bioRxiv - Biophysics 2020Quote: ... Type I solution from rat tail (Cat. Nr. C3867) and fibronectin (Cat. Nr. F1141) were purchased from Sigma-Aldrich.
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bioRxiv - Developmental Biology 2022Quote: ... tissue sections were probed overnight at 4°C with primary antibodies including rat α-Endomucin (1:250; Millipore; MAB2624); mouse α-Cardiac Troponin T (1:250 ...
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bioRxiv - Molecular Biology 2022Quote: ... 8-week-old rats received a single intravenous dose of 60 mg/kg streptozotocin (STZ, Sigma Chemical, MO, USA) diluted in STZ-vehicle (cold citrate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were incubated overnight at 37°C with primary antibody rat anti-MBP (1:1,000, MAB386, RRID: AB_94975, Millipore, Germany) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... and B35 rat neuroblastoma cells were differentiated in Dulbecco’s Modified Eagle (DME) media containing 0.1 mM dbcAMP (Sigma-Aldrich) and N2 supplement (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... One week after tracer application we deeply anesthetized the rat with urethane and performed a transcardial perfusion with 0.05M PBS followed by 4% PFA (Sigma Millipore). We harvested the brain and trigeminal ganglia ...
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bioRxiv - Neuroscience 2021Quote: Rats were subcutaneously injected with NMDA receptor co-agonist D-SER (s.c. D-SER 10mg/Kg, Sigma-Aldrich, Ireland) in vehicle ...
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bioRxiv - Cell Biology 2020Quote: ... incubated overnight at 4 °C in primary antibody: rat anti-Laminin-α2 (1:250, Cat. No L0663, Sigma-Aldrich), rabbit anti-Collagen Type I (1:250 ...
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bioRxiv - Neuroscience 2021Quote: Rat and mouse primary cortical neurons and BE(2)-M17 cells were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS pH 7.4 for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... frozen tissue (from 2–3 mice or 1 rat) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were immunoblotted with rabbit monoclonal anti-mouse caveolin-1 and rat monoclonal anti-mouse total β1-integrin (clone MB1.2, MAB1997 Millipore) as a loading control and a marker for PM fraction ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... for the rat Liver-Chip we used a mixture of collagen type IV (Sigma-Aldrich, St. Louis, MO, USA) and fibronectin (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: Distribution of EVs in rat brains was analyzed 18 h after intranasal administration of fluorescent EVs (PKH26-mini, Sigma)31 ...
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bioRxiv - Biophysics 2020Quote: Full-length cDNA of rat VDAC1 (GenBank: BC072484, purchased from Open Biosystems) was first subcloned into pET21a vector (Novagen) to generate pET-VDAC1 (wt) ...
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bioRxiv - Pathology 2021Quote: Total RNA was extracted from the kidney of a corticosteroid-clamped PAN rat following the Tri Reagent protocol (Sigma), Dnase I-treated ...
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bioRxiv - Systems Biology 2020Quote: ... Dll1 was detected with a 1:50 dilution of rat anti-Dll1 antibody (PGPM-1F9, MABN2284 from Sigma-Aldrich). After incubation the cultures were washed three times with 1% Triton-X-100 ...
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bioRxiv - Neuroscience 2020Quote: Organotypic hippocampal slices (350-µm) were prepared from male Wistar rats (P7) and cultured on Millicell CM membranes (Millipore). Slices were maintained in culture media (Life Technologies/Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... horseradish peroxidase-conjugated rabbit anti-rat IgG and horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma, St. Louis, MO) were used for Western blotting at dilutions of 1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... 1:100; goat anti-SOX2 (Santa Cruz Biotechnology sc-17320, 1:100; rat ant-SOMATOSTATIN (SST, EMD Millipore MAB354), 1:100 ...
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bioRxiv - Pathology 2021Quote: ... rats were injected intravenously via tail vein with a single low dose of streptozotocin (STZ) (35 mg/kg) (Sigma). One week after STZ injection ...
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bioRxiv - Cell Biology 2020Quote: ... on collagen coated plastic (50 μg/cm2 of rat tail collagen Type IV (SERVA, 47256.01) in 0.02N acetic acid(Sigma; 537020)) ...
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bioRxiv - Cell Biology 2021Quote: ... rat anti-mouse RIPK3 (WEHI clone 8G7; produced in-house7 and soon available from Millipore as MABC1595; 1:2000); rabbit anti-phospho-T231/S232 mouse RIPK3 (Genentech ...
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bioRxiv - Cell Biology 2021Quote: ... Then the gel was washed twice with PBS and coated with 2 ml of 0.2 mg ml−1 rat tail collagen type I (Millipore) at 4 °C overnight.
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bioRxiv - Neuroscience 2020Quote: ... The amounts injected were as follows: 0.01 or 0.1 mg/kg rat α-CGRP (Sigma-Aldrich, St Louis, MO), 0.25 or 2.5 mg/kg sodium nitroprusside (SNP ...
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bioRxiv - Microbiology 2022Quote: ... The rest of the supernatant was incubated O/N at 4°C with 8µg (per condition) of anti-NUP98 (rat, N1038 – Sigma). 62.5µl of A/G beads per condition were added and incubated for 2h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the pregnant rats were intraperitoneally (ip) injected with 1mL/Kg or LPS 0111:B4 (L2630; Lot# 114M4009V, Sigma-Aldrich) at a dose of 50µg/Kg and volume of 1mL/Kg diluted in sterile saline solution (0.9% NaCl solution ...
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bioRxiv - Neuroscience 2022Quote: ... extracted rat neonates (P0) hippocampi were dissociated and plated onto 12 mm poly-D-lysine (Sigma-Aldrich, Cat. #P6407)-treated glass coverslips ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... KIM1 and clusterin values were obtained utilizing MILLIPLEX MAP Rat Kidney Toxicity Magnetic Bead Panel 1 (Millipore Sigma, USA) without dilution ...
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bioRxiv - Microbiology 2024Quote: ... Horseradish peroxidase-conjugated rabbit anti-rat IgG and horseradish peroxidase-conjugated goat anti-chicken IgY (Sigma, St. Louis, MO) were used for western blotting at a dilution of 1:80,000 ...
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bioRxiv - Neuroscience 2022Quote: ... rats were anesthetized with an intraperitoneal injection of 50 mg/kg sodium pentobarbital (Sigma-Aldrich, St. Louis, MO, USA). An incision of approximately 1 cm in length was made along the midline ...
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bioRxiv - Neuroscience 2024Quote: ... Rats underwent bilateral infusion with a volume of 500 nl of either muscimol (400 ng/µl) (Sigma-Aldrich, M1523), gabazine (25 pmol ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were employed for visualization: rat anti-HA for PfCoronin-smHA (1:50; Sigma Cat. No. 11867423001), Sytox Far Red for DNA (ThermoFisher Cat ...
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bioRxiv - Microbiology 2024Quote: To assess localisation immunofluorescence assays were performed as described previously (31) using anti-HA (1:500, anti-rat,Sigma), anti-MYS (1:1000 ...