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Citations for Millipore Sigma :
2201 - 2250 of 2566 citations for Recombinant Rat Crp His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Signal-positive cells were detected by the following secondary antibodies: anti-rat Cy3 (Millipore, cat#AP189C, 1:250), anti-chicken Alexa Fluor 488 (Invitrogen cat#A11039 ...
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bioRxiv - Neuroscience 2021Quote: ... rats were injected intraperitoneally with 100 mg/kg 5-Bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI) dissolved in warm 0.9% saline immediately after release from Decapicone bags ...
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bioRxiv - Neuroscience 2019Quote: ... P0 rat hippocampal neurons were plated to poly-D-lysine (PDL; 10μg/cm2; Millipore Cat# A-003-E) coated coverslips at 5×104 neurons per well in a 24-well plate ...
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bioRxiv - Microbiology 2020Quote: ... rat monoclonal anti-CD45 antibody at a dilution of 1:100 (05-1416, Millipore Sigma, Burlington, MA, USA), rabbit polyclonal anti-CD68 at a dilution of 1:200 (ab125212 ...
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bioRxiv - Biochemistry 2022Quote: ... Colo205 cells were densely plated on 96-well plates precoated with 0.1μg/mL rat-tail collagen (Sigma-Aldrich) overnight and treated with activating concentrations of Fabs for 5 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The rats received daily IV infusions of the antibiotic Gentamycin (4 mg/kg, Sigma-Aldrich, St. Louis, MO) for seven days ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) or goat anti-Iba1 (1:1000 ...
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bioRxiv - Systems Biology 2021Quote: ... we plated 10k – 30k cells on 12 mm collagen-coated glass coverslips (rat tail collagen, Sigma, #122-20). Cells were plated the afternoon prior to imaging ...
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bioRxiv - Neuroscience 2022Quote: ND7/23 cells (a mouse neuroblastoma × rat dorsal root ganglion neuron hybrid cell line; Sigma−Aldrich, ECACC 92090903), were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Neuroscience 2019Quote: ... rat NSPCs cells were lysed and the DNA was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) (Sigma-Aldrich), purified using Purelink Genomic DNA kits (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... falciparum pv5-3xHA:loxP parasites that were fixed in 4% formaldehyde using rat anti-HA (1:500; Sigma Aldrich) and rabbit anti-SERA5 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Pregnant rats were treated intraperitoneally (i.p.) with DBP (99.5% pure, solid, Sigma Chemical Co., St. Louis, MO, USA) in corn oil (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and mixed with Milliplex MAP rat kidney toxicity magnetic bead panel 1 (EMD Millipore Corporation, Charles, MO, USA). A separate standard curve was prepared and run with each assay plate ...
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bioRxiv - Neuroscience 2020Quote: ... whereby each rat was injected twice in a pseudorandomized order with either ketamine 20 mg/kg (Sigma, Poland) or saline ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-human RIPK3 (clone 1H2; produced in-house5 and available as Millipore MABC1640; 1:2000/1:400), rabbit anti-phospho-S227 human RIPK3 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Genetics 2022Quote: All rats received intraperitoneal injection of calcein (10mg/kg body mass, Sigma-Aldrich, Co., St. Louis, MO, USA) for histomorphometric analysis on the second and the 6th day before euthanization ...
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bioRxiv - Pathology 2022Quote: ... respectively an anti-rat IgG conjugated to alkaline phosphatase (1:500) (Sigma-Aldrich®, Merck KGaA, Darmstadt, Germany), for 60 minutes ...
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bioRxiv - Neuroscience 2022Quote: Rats were anesthesia by intraperitoneal injection with 50 mg/kg sodium pentobarbital (Sigma-Aldrich, St. Louis, MO, USA). Hair was removed from the neck’s midline ...
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bioRxiv - Neuroscience 2023Quote: ... Thirty minutes prior to infusion rats were pretreated with intraperitoneal (IP) injections of 50mg/kg pargyline (Sigma-Aldrich) and 5mg/kg desipramine (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and mixed with Milliplex MAP rat kidney toxicity magnetic bead panel 1 (EMD Millipore Corporation, Charles, MO, USA). For each 96-well plate ...
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bioRxiv - Neuroscience 2022Quote: ... were stained using standard staining protocol (primary antibodies: Mouse monoclonal anti-TH [Immunostar Cat#: 22941 RRID:AB_572268], Rat monoclonal anti-DAT [Millipore Cat# ...
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bioRxiv - Microbiology 2024Quote: ... Immunoblot analysis after SDS-PAGE was performed with relevant primary antibodies: anti-HA (1:500, anti-rat, Sigma), anti-TOM40 (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and mixed with Milliplex MAP rat kidney toxicity magnetic bead panel 1 (EMD Millipore Corporation, Charles, MO, USA). For each 96-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti-integrin-β1 (mAB13, MABT821), rabbit anti-integrin β1 (AB1952P), mouse anti-integrin β1 (12G10, MAB2247) (Millipore); rabbit-anti-integrin-β3 (A2542) ...
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bioRxiv - Developmental Biology 2023Quote: ... In some instance E16 peripheral rat retinal cells were treated with chondroitinase ABC (0.2 U/ml; Sigma-Aldrich) to degrade CSPG before culture ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the rats received daily infusions of the antibiotic Gentamycin (4 mg/kg, IV, Sigma-Aldrich, St. Louis, MO). A sterile heparin solution (0.1 ml ...
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bioRxiv - Developmental Biology 2023Quote: ... pregnant Sprague-Dawley rats received 100mg of nitrofen (2,4-Dichlorophenyl 4-nitrophenyl ether; Sigma CAS#1836-75-5) in 1mL of olive oil at E9.5 to induce fetal pulmonary hypoplasia ...
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bioRxiv - Cancer Biology 2023Quote: ... pre-coated with collagen type I rat tail (1:10 diluted in sterile water, Sigma, #9007-34-5), and the day after ...
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bioRxiv - Biophysics 2024Quote: ... 0.025 µg/µl of fibronectin (Chem Cruz) or 0.25 µg/µl of rat tail Type I collagen (Millipore) were added onto the cassette immediately after plasma cleaning ...
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bioRxiv - Cell Biology 2021Quote: ... P3 2M (AMS biotechnology #GSC-6201G 2M or #GSC-6101G 7M) and LIF ESGRO® Recombinant Mouse LIF Protein (1000 units/mL) (Millipore # ESG1107). For induction of the transgene Stemolecule Doxycycline hyclate 10 mg (Stemgent#04-0016 ...
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biophysics 2020Quote: Both unlabeled and 15N labeled recombinant full-length αS was expressed and purified in Rosetta™ 2(DE3) pLysS (EMD Millipore®) cells using IMPACT™ system protocol (New England Biolabs® ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).