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Citations for Millipore Sigma :
2301 - 2350 of 2566 citations for Recombinant Rat Crp His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used at the indicated dilutions: rat anti-α-tubulin 1:500 (Sigma Aldrich, MAB1864), rabbit anti-GFP 1:1000 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used at the indicated dilutions: rat anti-α-tubulin 1:500 (Sigma Aldrich, MAB1864), rabbit anti-KIF18A 1:100 (Bethyl ...
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bioRxiv - Neuroscience 2023Quote: ... All brain slices containing the ventral mesencephalon were used for immunolabeling with DAT (rat monoclonal, 1/4000, Millipore, USA) and TH antibodies (rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... Rat anti-Ago2 (cat no. SAB4200085) and goat anti-Apolipoprotein B (cat no. AB742) were purchased from Millipore Sigma. Rabbit anti-CD63 (cat no ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNAs encoding human AMPKα1-KD (aa 27-290) (ref. 164) and rat CaMKK2-KD42 were inserted into the pET-28a (Novagen) vectors for expressing His-tagged recombinant proteins as described previously ...
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bioRxiv - Biophysics 2023Quote: ... The suspended cells in DMEM were mixed with rat tail-derived type I collagen in 0.1% acetic acid and 2X DMEM (Sigma). After neutralizing the pH with sodium hydroxide ...
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bioRxiv - Molecular Biology 2023Quote: Hippocampal tissue from P2 Wister rats was chopped to small pieces and digested by 5mg/ml trypsin (Sigma #T1005) - 5mg/ml DNaseI for 5 min ...
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bioRxiv - Physiology 2023Quote: ... and with a rat monoclonal anti-Septin 7 (1:100 dilution, clone 19A4, MABT 1557, Millipore Sigma, Burlington, MA) in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... Prior to seeding HepG2 cells, wells and coverslips were surface-coated with collagen (type I, rat tail) (Sigma-Aldrich) in a 50 µg/mL solution in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-5 mm3 biopsies from naked mole-rat or shaved mouse back skin were incubated with either: I) dissociation buffer (1.25 mg/ml collagenase I (Sigma), 0.5 mg/ml collagenase II (Worthington) ...
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bioRxiv - Cell Biology 2023Quote: Coverslips with either adult mouse or neonatal rat cardiomyocytes were incubated 1h with anti-α-actinin antibody (A7811, Sigma) at room temperature ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: The following primary antibodies were used in this study: rat monoclonal to MBP at 1/250 (#MAB386; Sigma-Aldrich), rabbit polyclonal to Caspr at 1/50 (#ab34151 ...
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bioRxiv - Microbiology 2024Quote: ... fd1-cas9 schizonts derived from infected rats were harvested after 22 hr culture and purified on a Nycodenz (Sigma) gradient ...
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bioRxiv - Molecular Biology 2024Quote: 96-well flat-bottomed tissue culture plates (Earthox) were coated with varying concentrations of rat tail collagen I (Sigma) or fibronectin (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Genomics 2022Quote: ... in a 12-well cell culture plate coated with Human recombinant laminin 511 (BioLamina, Cat. LN511-0202) and fixed in 1xPBS with 3.7% formaldehyde (Millipore Sigma, Cat. F8775-25ML) for 10 mins and then permeabilized in ice-cold 70% (vol./vol. ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Cell Biology 2023Quote: ... East Brunswick, NJ, USA), human recombinant Stem Cell Factor (100ng/mL, supernatant SCF producing cell line) and dexamethasone (1μM; Sigma, St. Louis, MO, USA).5 EBL cultures were kept at a density of 0.7-1.5 million/mL by dilution with fresh medium ...
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bioRxiv - Microbiology 2023Quote: ... Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Microbiology 2024Quote: ... for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for QD8 production ...
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bioRxiv - Neuroscience 2024Quote: ... we used our LPS-sensitized model of HI described above and delivered IP 1.5 IU/g of hCG diluted in normal saline (Sigma-Aldrich, St. Louis, MO, Catalog no. CG-10) or normal saline alone as vehicle control two hours prior to LPS administration (Figure 2A) ...
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bioRxiv - Systems Biology 2020Quote: ... mouse and naked mole rat fibroblasts were cultured as described earlier and treated with varying concentrations of AZC (Sigma-Aldrich). After 5 days of treatment ...
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bioRxiv - Cell Biology 2019Quote: Samples were assayed for insulin or proinsulin content using ELISA according to the instructions of the manufacturers (Rat/Mouse insulin ELISA, Millipore, #EZRMI-13K ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies (monoclonal α-HA rat 3F10 and monoclonal α-myc mouse 9E10, from Roche, distributed by Sigma Aldrich) were diluted 1:1000 and 1:500 respectively in PBS + 0.4 % BSA and the samples incubated for 1 hour at RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... In the first experiment, rats were injected with either sterile saline (vehicle; 1 mL/kg, i.p.) or propranolol hydrochloride (PROP, Sigma-Aldrich, St Louis ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then fixed and rat anti-MBP (1/5) [11] and rabbit anti-NF-H (1/1000, EMD Millipore) primary antibodies were added o/n at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... antibodies, and the secondary antibodies used included anti-rabbit (31460, Pierce), anti-mouse (31430, Pierce) and anti-rat (A9037, Sigma) HPR antibodies ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...
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bioRxiv - Neuroscience 2019Quote: ... Western blotting was performed using an antibody directed towards rat kisspeptin (1:500; EMD Millipore AB9754; Billerica, MA; Table 2). This antibody has been extensively characterized (Desroziers et al. ...
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bioRxiv - Molecular Biology 2021Quote: Type 1 diabetes was induced in male Sprague Dawley rats at 8 weeks of age via a single 55mg/kg tail vein injection of streptozotocin (STZ; Sigma), and monitored for 8 weeks ...
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bioRxiv - Neuroscience 2020Quote: Immunofluorescence was done as previously described (Yellajoshyula et al., 2017) for the following antibodies: Rat anti-MBP (Millipore, Cat# MAB386), Mouse anti-O4 (kindly provided by Dr ...
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bioRxiv - Neuroscience 2021Quote: ... In three rats brain stem sections were first immunoreacted with a goat anti-choline acetyltransferase antibody (1:1000; Millipore Sigma) and a rabbit anti-goat IgG conjugated to horseradish peroxidase (Vector Labs) ...
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bioRxiv - Developmental Biology 2020Quote: ... cryosections were incubated overnight at 4°C with the following primary antibodies: rat monoclonal anti-Neural Cell Adhesion Molecule L1 (1:500 Millipore, Cat # MAB5272 ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Plant Biology 2021Quote: ... LM19, LM2, JIM8 and JIM13 (PlantProbes, Leeds, UK) were used as primary antibodies and FITC–conjugated anti-rat IgG (Sigma) as secondary antibody in all cases ...
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bioRxiv - Biochemistry 2020Quote: ... The cells and media were separated after the incubation and processed for quantification of insulin levels using the RI-13K Rat Insulin RIA Kit (Millipore) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217, 1:1,000; chicken anti-GFP, Life Technologies A10262, 1:1,000; rabbit anti-vasopressin, Sigma-Aldrich AB1565 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C in blocking buffer with the following primary antibodies: rat anti-MBP (monoclonal, 1:100, Millipore), rabbit anti-Parvalbumin (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Insulin concentration of the supernatants was measured with a mouse/rat insulin ELISA (EZRMI-13K, EMD Millipore, Billerica, MA, USA).
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bioRxiv - Physiology 2020Quote: ... and each circulating hormones were determined using a commercially available ELISA kit for total ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-91K, Millipore), and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit ...
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bioRxiv - Neuroscience 2022Quote: ... blocked in PBST and 10% NGS for 2–3 hours at room temperature, and incubated in rat anti-mCherry antibody (1:2000, Life Sciences) and rabbit anti-TH antibody (1:1000, Millipore) at 4°C for four nights ...