Labshake search
Citations for Millipore Sigma :
2101 - 2150 of 2566 citations for Recombinant Rat Crp His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
-
bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
-
bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
-
bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
-
bioRxiv - Developmental Biology 2024Quote: Bovine OVGP1 (bOVGP1) and murine OVGP1 (mOVGP1) recombinant proteins were purified using the Amicon Pro Purification System (Merck Millipore Ltd) by combining metal chelation chromatography with an Amicon concentrator membrane ...
-
bioRxiv - Systems Biology 2024Quote: ... 60µg of total protein per sample and 5µg recombinant Tau (rTau) (2N4RTau and 1N3R Tau) were digested with either Trypsin Protease (Pierce) or LysargiNase (EMD Millipore) at 1:50 w/w overnight at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... primary antibody followed by polyclonal anti-rat IgG secondary antibody conjugated CF-647 (Sigma, catalogue no. SAB4600186). On-chip cell imaging was carried on with a long-distance 25x objective (NA=1.1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... the membranes were incubated with HRP-conjugated anti-rat secondary antibody (1:5000 dilution, Sigma-Aldrich A9037) in 0.1 % (w/v ...
-
bioRxiv - Immunology 2021Quote: ... the receptors were crosslinked by the addition of anti-mouse or anti-rat IgG Alexa Fluor (Sigma) during 15 min on ice.
-
bioRxiv - Cell Biology 2021Quote: ... Western blots were done with a rat monoclonal anti-HA high-affinity antibody (clone 3F10, Millipore Sigma), a rabbit polyclonal anti-histone H3 antibody (ab1791 ...
-
bioRxiv - Neuroscience 2021Quote: Rat choroid plexus (lateral and 4th) was isolated in HEPES-aCSF and stored in RNAlater (R0901, Sigma) at −80 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... IL) and Rat DIMT1 siRNA (Cat# NM_001106408 (SASI_Rn02_00218668) was from (Sigma Aldrich, St. Louis, Missouri, United States) Fluorescent probes were supplied by Molecular Probes (Rockford ...
-
bioRxiv - Molecular Biology 2021Quote: ... and isolated murine hepatocytes (muHEP) were seeded on rat-tail collagen (10 µg cm−1, Merck Millipore) coated well plates at a density of 0.1×106 cells per cm² ...
-
bioRxiv - Cell Biology 2021Quote: ... MTs were labeled with rat monoclonal antibody YL 1/2 directed against tyrosinated -tubulin (1:1000, Millipore), or rabbit polyclonal antibody against detyrosinated -tubulin (1:1000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Monoclonal rat E-cadherin antibody that specifically binds to the extracellular domain of E-cadherin (Sigma, U3254) was used at 1:1600 dilution ...
-
bioRxiv - Neuroscience 2022Quote: ... Twenty-two rats were injected intraperitoneally (i.p.) with Ecoli LPS (250 μg/kg, serotype 0127:B8, SIGMA) diluted in sterile endotoxin-free isotonic saline 24h before LTE or sham-exposure (n= 11 per group).
-
bioRxiv - Bioengineering 2022Quote: ... Cells were seeded in rat tail type I collagen (100 µg/mL, Sigma Aldrich, St. Louis, MO)-coated flasks and maintained in modified Endothelial Growth Media 2 ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cover slides were incubated at room temperature with 10 µg/ml rat-tail collagen-I (Sigma) for 3 hours ...
-
bioRxiv - Cell Biology 2022Quote: The following primary antibodies were used: rat monoclonal anti-mouse total β1-integrin (clone MB1.2, MAB1997 Millipore); rat monoclonal anti-mouse β1-integrin ...
-
bioRxiv - Neuroscience 2019Quote: ... the slices were incubated with either rat anti-Sst antibody (1:100, MAB354, Millipore, Burlington, MA, USA) or guinea pig anti-PV antibody (1:500 ...
-
bioRxiv - Bioengineering 2020Quote: ... Multiplexed chemokine and cytokine analysis was performed using Milliplex MAP Rat Cytokine/Chemokine Magnetic kit (Millipore Sigma) and analyzed using a MAGPIX Luminex instrument (Luminex) ...
-
bioRxiv - Immunology 2021Quote: ... Rat hearts were divided into groups of K-H buffer (control, n=7) and LPS (Sigma-Aldrich, #L2880 ...
-
bioRxiv - Immunology 2020Quote: ... Each rat received an intraperitoneal injection of 300 ng Pertussis toxin (Sigma-Aldrich, St. Louis, MO, USA) in 0.1 ml distilled water immediately after the subcutaneous injection and again 48hours later ...
-
bioRxiv - Immunology 2020Quote: ... intracellular staining was performed by 5 min pre-incubation with rat IgG in 0.5% saponin (Sigma-Aldrich) and addition of anti-CD154 antibody and anti-cytokine antibodies for another 30 min in 0.5% saponin at RT.
-
bioRxiv - Molecular Biology 2023Quote: ... hepatocytes were isolated after serial perfusion of rat liver by Hanks ‘ balanced salt solution (HBSS, Sigma, H6648) and collagenase CLS type II solution (50 mg/150 mL ...
-
bioRxiv - Molecular Biology 2023Quote: ... anti-rabbit or anti-rat alkaline phosphatase-conjugated secondary antibodies (dilution 1:10,000, Sigma-Aldrich; Taufkirchen, DE) in 3% (w/v ...
-
Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... Insulin serum was measured using ELISA (Rat/Mouse Insulin ELISA, #EZRMI-13K, Millipore St. Charles, MO, USA)) ...
-
bioRxiv - Physiology 2022Quote: ... Cells were plated onto glass coverslips coated with collagen (type I rat tail, Sigma; 10 μg/cm2) and maintained in a humidified atmosphere of 5% CO2 and 95% air at 37 °C in Williams’ Medium E supplemented with penicillin ...
-
bioRxiv - Biophysics 2022Quote: ... For E-Cadherin stainings a 1:1000 dilution of Alexa 647-conjugated anti-rat (Sigma-Aldrich SAB4600186) was used ...
-
bioRxiv - Biophysics 2022Quote: ... and then incubated for 1h in BSA-PBST with a goat anti-rat CF-568 (Sigma SAb4600086) secondary antibodies ...
-
bioRxiv - Neuroscience 2023Quote: ... rats were fasted for 18 hours before experimentation and anesthetized with Inactin (Sigma T133; 150mg/kg i.p.). A tracheotomy was performed prior to making a midline abdominal incision to expose the ventral stomach ...
-
Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... the Snapwell inserts were coated with a 1:1 ratio of rat tail collagen (Sigma Aldrich, USA) and ethanol (VWR Chemicals ...
-
bioRxiv - Neuroscience 2023Quote: SD rats (5-week-old males) were anaesthetized with sodium pentobarbital (Sigma, 40 mg/kg, intraperitoneal injection). The brain is quickly removed and cooled in ice-cold modified artificial cerebrospinal fluid (ACSF) ...
-
bioRxiv - Microbiology 2023Quote: ... anti-rabbit or anti-rat alkaline phosphatase-conjugated secondary antibodies (dilution 1:10,000, Sigma-Aldrich; Taufkirchen, DE) in 3% (w/v ...
-
bioRxiv - Immunology 2023Quote: Cell seeding on transwell inserts: 120,000 T84 cells were seeded on rat-collagen (Sigma-Aldrich #C7667-25MG) coated 6.5 mm transwell 3.0 µm Pore Polycarbonate Membrane Inserts (Corning ...
-
bioRxiv - Cell Biology 2023Quote: ... the rats were anesthetized by intraperitoneal injection of 10 mg/kg sodium pentobarbital (Sigma□Aldrich, MO, USA). Next ...
-
bioRxiv - Cell Biology 2023Quote: ... The AP-AKI rat model was created by retrograde injection of sodium taurocholate (Sigma□Aldrich, MO, USA) into the pancreatic duct ...
-
bioRxiv - Developmental Biology 2022Quote: Pregnant rats were intraperitoneally injected with poly I:C (10 mg/kg body weight, P1530-25MG, Sigma-Aldrich) on gd 13.5 ...
-
bioRxiv - Neuroscience 2023Quote: ... male and female rat pups were injected with 5µL of 1% carrageenan (CGN; Sigma, St. Louis MO) dissolved in sterile saline into the intraplantar surface of the right hind paw.
-
bioRxiv - Neuroscience 2023Quote: ... rats underwent three conditioning trials to associate a 0.1% saccharin solution (Sigma Aldrich, Missouri, in tap water) with drug injection ...
-
bioRxiv - Neuroscience 2024Quote: ... Each sample was labeled with 5μg mouse anti-NeuN (RRID:AB_2572267) and 4μg rabbit anti-parvalbumin (Swant PV27; 1:400) and rat anti-somatostatin (Millipore) followed by fluorescent secondary antibodies ...
-
bioRxiv - Neuroscience 2023Quote: Neonatal Wistar rats were administered either vehicle (phosphate buffered saline; PBS) or cisplatin (Sigma-Aldrich; 0.3mg/kg) via intraperitoneal injection delivered on 2 occasions ...
-
bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate ...
-
bioRxiv - Physiology 2024Quote: ... Rats were then given neuromuscular block with pancuronium bromide (2 mg/kg; Sigma-Aldrich, St. Louis, MO).
-
bioRxiv - Neuroscience 2024Quote: Rats were deeply anesthetized with isoflurane and transcardially perfused with cold phosphate-buffered saline (PBS, Sigma, USA), followed by cold 4% paraformaldehyde (PFA ...
-
Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
-
bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...