Labshake search
Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 5 x 106 – 1 x 107 cell equivalents were analyzed on PAA / SDS gels and probed with anti-HA antibodies (Sigma, rat 3F10, 1:1000), anti-Myc antibodies (ThermoFisher ...
-
bioRxiv - Cancer Biology 2019Quote: ... The total protein was dissolved by SDS-PAGE loading buffer and transferred onto poly vinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). The primary antibodies (Table 3 ...
-
bioRxiv - Synthetic Biology 2019Quote: Cells were lysed in ice-cold cell lysis buffer and equal amounts of the protein were loaded for electrophoresis on denaturing SDS/PAGE gel and then transferred onto PVDF membranes (Millipore, Billerica, MA). The blots were probed with the primary antibodies overnight at 4 °C ...
-
bioRxiv - Cell Biology 2019Quote: ... Gels were then process for mass spectrometry or the proteins were transferred to the polyvinylidene difluoride membrane (Merck Millipore, Cat. No. IPVH00010) by Western Blotting (WB) ...
-
bioRxiv - Plant Biology 2020Quote: ... An efficient transfer of proteins was determined by staining a membrane with Ponceau S solution (Sigma - Aldrich, Cat No 6226-79-5). Afterwards a membrane was incubated for 1 h in a blocking buffer shaking at room temperature followed by binding with primary antibody in 5 % BSA/TBS-T buffer overnight at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... Protein samples were separated in 4-15% polyacrylamide gels and transferred to 0.2 µm Immobilion-PSQ transfer membrane (Millipore, Cat. No ISEQ00010) using a Bio-Rad transfer system ...
-
bioRxiv - Cell Biology 2021Quote: ... About 20 ug total proteins in each sample were fractionated by 10% SDS PAGE gel and transferred to PVDF membranes (Millipore Corporation, USA) and immunoblotted with primary antibody anti-GFP (Invitrogen-Molecular Probes ...
-
bioRxiv - Cancer Biology 2021Quote: Proteins were separated using a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred onto Immobilon-FL PVDF membrane (Millipore). The membranes were blocked in 5% BSA for 1 hour ...
-
bioRxiv - Cancer Biology 2021Quote: ... 15 μg of tumor protein was resolved on 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membrane (Millipore, catalog# IPFL00010). Membranes were blocked with 5% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... the proteins were wet transferred onto ‘Amersham™ Protran™0.2 µm NC’ nitrocellulose membranes (GE10600002 Sigma-Aldrich Handels Gmbh, Vienna, Austria) at constant 80 V for 1,5 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... each sample with equal amount of protein was subjected to SDS-PAGE gel (10% or 15%) and transferred to a PVDF membrane (Millipore, Massachusetts, USA). The membrane was incubated with the specific primary antibody overnight at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Thirty to forty-five micrograms of the protein were separated on a 5-12% Tris-acetate gel and electrophoretically transferred to PVDF membranes (Millipore, Billerica, MA). Membranes where then incubated with the primary antibodies against sXbp1 (BioLegend) ...
-
bioRxiv - Pathology 2021Quote: ... 20 µg of total protein was separated by electrophoresis in a 10% SDS polyacrylamide gel and transferred to a PVDF membrane (Millipore, Billerica, MA) for western blot analysis.
-
bioRxiv - Neuroscience 2019Quote: ... Equal amount of proteins (20 µg/lane) was separated by SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA). Membranes were blocked for 1 h in 5% nonfat dried milk in either 0.1% Tween20 in PBS (PBST ...
-
bioRxiv - Cell Biology 2021Quote: ... 8% SDS-PAGE was used for protein separation by electrophoresis and transferred to a PVDF membrane (Immobilon®-P, Millipore Corporation, IPVH00010). 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
-
bioRxiv - Genomics 2021Quote: ... Total protein (40 μ g) was electrophoresed on a 10% polyacrylamide gel by SDS-PAGE and transferred to PVDF membranes (Millipore, USA), followed by blocking with 5% milk for 2 h ...
-
bioRxiv - Developmental Biology 2022Quote: ... and aliquots of 15 μg protein were separated by SDS-PAGE and blotted onto a polyvinylidene fluoride (PVDF) membrane (Millipore, MA, USA). Membranes were blocked at room temperature for 15 min in QuickBlock™ Western (Beyotime) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skim milk in TBST and target proteins were detected with ANTI-FLAG® M2 antibody (Sigma-Aldrich) and anti-α-tubulin (SantaCruz).
-
bioRxiv - Biophysics 2022Quote: ... The supernatant that contained the soluble fusion protein was filtered over Millex-GP syringe-driven 0.22-µm PES membranes (Millipore Sigma, Burlington, MD). The fusion protein was purified by fast protein liquid chromatography (FPLC ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were then run on NuPAGE 4-12% Bis-Tris Protein Gels (Thermo #NP0335BOX) and transferred to a PVDF membrane (EMD Millipore #IPVH00005). Blots were probed with primary antibodies overnight at 4°C in 5% blocking solution ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein samples were separated on ammediol SDS-PAGE [30] and transferred to a PVDF membrane (polyvinylidene difluoride, EMD Millipore, Burlington, MA, USA) in transfer buffer (10 mM N-cyclohexyl-3-aminopropanesulfonic acid ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 µg proteins were separated by SDS-PAGE (10– 12% gels) and were blotted onto a nitrocellulose membrane (Millipore, Billerica, MA, USA). The membrane was blocked with 5% BSA in TBST at room temperature for 1h ...
-
Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
-
bioRxiv - Microbiology 2023Quote: Proteins in the cell and viral lysates (5 μg of total protein and 10 ng of p24 antigen) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat# IPVH00010). Membranes were blocked with 5% milk in PBS containing 0.1% Tween 20 (0.1% PBST ...
-
bioRxiv - Microbiology 2023Quote: ... Protein samples were separated by SDS-PAGE using 10% agarose gel and transferred to transfer membranes (Millipore-Sigma, St. Louis, MO, USA), which were incubated in 5% nonfat milk (A600669 ...
-
bioRxiv - Microbiology 2023Quote: ... Protein samples were separated by SDS-PAGE using 10% agarose gel and transferred to transfer membranes (Millipore-Sigma, St. Louis, MO, USA), which were incubated in 5% nonfat milk (A600669 ...
-
bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
-
ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... SDS samples containing 4∼8 μg total proteins were separated with 12% HEPES-Tris PAGE gel (MEILUNBIO, MA0243) and transferred to polyvinylidene difluoride membranes (Millipore, 70584-3). The membrane was blocked with 5% skim milk in TBST buffer (20mM Tris ...
-
bioRxiv - Pathology 2023Quote: ... An equal amount of protein (10 µg) was subsequently separated by 12% SDS-PAGE and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Bedford, MA). The membrane was blocked with a 5% skimmed milk solution prepared in Tris-buffered saline containing 0.1% Tween-20 (TBS-T ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The prepared proteins were separated by SDS-PAGE gel electrophoresis (Yazyme, Shanghai, China) and transferred to activated PVDF membranes (Millipore, MA, USA). The PVDF membranes were then gently rinsed with TBST and placed in 7% skim milk powder solution and closed on a shaker at room temperature for 3 h ...
-
bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
-
bioRxiv - Physiology 2024Quote: ... 20 µg total protein extracts were separated by 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and cut into 2-3 parts ...
-
bioRxiv - Immunology 2019Quote: ... or control polyclonal rat IgG (Sigma Aldrich, I4131) and were given the same dose i.p ...
-
bioRxiv - Neuroscience 2019Quote: ... and Cy3-conjugated anti-rat IgG (AP136C, Millipore), in all cases diluted at 1:400 in buffer G for 2 h at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... mouse or rat HRP-conjugated secondary antibody (Sigma) diluted 1:4000 in 2.5% BSA in TBST for 45 min and washed again ...
-
bioRxiv - Physiology 2022Quote: ... 0.1 mg/ml rat albumin (Sigma Aldrich #A6414), pH 10.5 ...
-
bioRxiv - Microbiology 2022Quote: ... precoated with rat tail collagen type I (Sigma) at 200 µg/ml and incubated at 37°C overnight in DMEM (Gibco or Lonza ...
-
bioRxiv - Immunology 2020Quote: ... Control rat IgG was purchased from Sigma-Aldrich. Anti-PD-1 (G4 ...
-
bioRxiv - Cell Biology 2019Quote: ... rat anti-HA-tag (3F10, Sigma-Aldrich, #ROAHAHA), rabbit anti-Myc-tag (71D10 ...
-
bioRxiv - Neuroscience 2021Quote: Rat α-CGRP (Sigma-Aldrich, St. Louis, MO) was diluted in Dulbecco phosphate buffered solution (PBS ...
-
bioRxiv - Immunology 2021Quote: ... or an equivalent amount of rat IgG (Sigma) diluted in PBS by intraperitoneal (i.p. ...
-
bioRxiv - Cell Biology 2022Quote: ... Rat anti integrin αvβ3 was from Millipore(LM609).
-
bioRxiv - Biophysics 2022Quote: ... anti-Rat IgG were purchased from Sigma Aldrich, Japan.
-
bioRxiv - Biophysics 2022Quote: ... and Goat anti-rat CF 770 (Sigma SAb4600479) 1:250.
-
bioRxiv - Cell Biology 2024Quote: ... and anti-rat IgG antibody (A9037; Sigma-Aldrich) produced in goat ...
-
bioRxiv - Immunology 2024Quote: ... AP-conjugated Anti-rat IgG (A8438, Sigma-Aldrich) was used a secondary antibody ...
-
bioRxiv - Immunology 2023Quote: ... Antibody cocktails included normal rat serum (Sigma-Aldrich) and FcψRII/III (2.4G2 supernatant) ...
-
bioRxiv - Cancer Biology 2024Quote: ... rat anti-tyrosinated tubulin (Millipore Sigma, MAB1864-I), mouse anti-polyglutamylated tubulin (Millipore Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...