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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... The 1 mg/mL pure bovine glutamate dehydrogenase (GDH) protein (Sigma) was reacted with 1 mM DSSO in 50 mM HEPES buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Transferred proteins were probed with anti-tubulin (Sigma, 1:10,000 dilution) and anti-GFP (Takara Bio ...
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bioRxiv - Bioengineering 2021Quote: ... mouse anti-glial fibrillary acidic protein (GFAP)–Cy3 (Sigma, 1:2000) to show astrocyte structure ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Alzheimer Precursor Protein (APP) 22C11 (1:100, MAB348, Millipore), and mouse anti-β-Tub III/Tuj 1 (1:4000 ...
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bioRxiv - Cancer Biology 2020Quote: ... at 1 ug/ml or control protein Bovine Serum Albumin (Sigma) at 1 ug/ml in PBS were coated onto flat bottom ...
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bioRxiv - Plant Biology 2020Quote: ... The immunoprecipitated proteins were incubated with 1 μg MBP (Sigma-Aldrich) in kinase buffer (25 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... breast cancer resistance protein (Bcrp-1:100; Merck Millipore, Massachusetts, USA) or Abcg1 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... polyclonal goat anti-ribosomal protein L8 (SAB2500882, Sigma-Aldrich; 1:500), monoclonal mouse anti-β-Actin (GTX26276 ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... overnight to degrade proteins and then with 1 mM PMSF (Sigma) to inactivate the Proteinase K ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal Prosurfactant Protein C (1:200 pH9, AB3786, Merck Millipore), mouse monoclonal TTF-1 (clone SPT24 ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with mouse anti-FLAG monoclonal (Sigma, 1/10000), rabbit anti-Rad51 polyclonal (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... stained with 1:200 mouse anti-ubiquitinylated proteins (Sigma #04-263) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-phosphoepitope SR proteins clone1H4 (Millipore, cat#MABE50, 1:2,000 dilution) were used as primary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... protein lysates were prepared in 1× RIPA buffer (Millipore, #20-188) from Drosophila head tissue expressing UAS-FLAG-Dx ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Developmental Biology 2024Quote: ... MPM-2 Mitotic Protein Monoclonal mouse 1:100 (Millipore 05-368), Lamin mouse 1:300 (DSHB ADL67.10) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were removed by phenol:chloroform:isoamyl alcohol (25:24:1) (PCI) (Sigma) extraction followed by ethanol precipitation ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluates were resolved on a 10% SDS-PAGE gel and the proteins were electroblotted onto PVDF membranes (Millipore, Immobilon-P, Cat # IPVH20200) for probing with following primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Twenty micrograms of total protein were separated on 10% SDS- PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore Co., Bedford, MA). Protein transfer was checked by staining the membrane with Ponceau S red (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein was separated by 12% sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) electrophoresis and then was transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA), which was followed by incubation with antibodies against STAP1 ...
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bioRxiv - Cancer Biology 2019Quote: ... A total of 70 μg of protein was subjected to electrophoresis and then transferred to nitrocellulose membranes (Sigma Chemical Co., St. Louis, MO). The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal ...
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bioRxiv - Physiology 2019Quote: ... equal amounts of protein (in 1X Laemmli loading buffer) were resolved using SDS-PAGE gels and transferred to PVDF membranes (Millipore Cat. No# IPVH00010). After blocking in 5% BSA or 5% fat-free milk in TBST (TBS with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (30 μg) from the cell lysate were resolved by SDS–PAGE and transferred to Immobilon-P membranes (Millipore Corp., Billerica, MA, USA). Membranes were incubated with anti-NLRP3 (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... Aliquots were run on a 4–12% SDS-PAGE gradient gel and electrophoresed proteins were transferred to a PDVF membrane (Immobilon-P, Millipore, Burlington, MA, USA). To facilitate detection ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal volumes of protein samples were separated by SDS-poly acrylamide gel electrophoresis and electro-transferred to PVDF membranes (Millipore, Billerica, MA, USA). After blocked with 5% non-fat milk dissolved in TBST (10mM Tris ...
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bioRxiv - Plant Biology 2022Quote: ... normalizing protein levels to the Rubisco large subunit visualized in membranes stained with Ponceau (0.1 % Ponceau S Sigma-Aldrich in 5 % acetic acid). The statistical differences between the average protein levels of each sample were inferred using a Student’s t-test.
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bioRxiv - Molecular Biology 2022Quote: ... equal amounts of protein extracts were subjected to separation SDS-PAGE before being transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA), and subsequent visualization by Western blotting with distinct antibodies as indicated ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA). After blocked with 5% non-fat milk ...
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bioRxiv - Physiology 2022Quote: ... The protein extracts were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon membrane (EMD Millipore, Billerica, MA). The membrane was blocked with 5% nonfat milk for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were separated on 8% SDS-PAGE gels and transferred to a PVDF membrane (immobilon FL; Sigma-Aldrich, St Louis, MO, USA). Membranes were blocked with 1% (for TMEM16A protein ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). The antibodies are listed in Supplementary Table 4 ...
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bioRxiv - Biophysics 2022Quote: ... The imidazole was removed from the purified protein using an Amicon Ultra centrifugal filter with a regenerated cellulose membrane (Millipore Sigma, Burlington, MA). At least 3 washes with imidazole-free PBS buffer were done ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The imidazole was removed from the purified protein using an Amicon Ultra centrifugal filter with a regenerated cellulose membrane (Millipore Sigma, Burlington, MA). At least 3 washes with imidazole-free PBS buffer were done ...
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bioRxiv - Physiology 2023Quote: ... Equal amounts of protein extracts were subjected to electrophoresis in 8–12% SDS- polyacrylamide gels and then transferred to PVDF membranes (EMD Millipore, Burlintgon, MA). The membranes were blocked with 5% non-fat milk at room temperature and then incubated with relevant primary antibodies at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins were resolved by electrophoresis on SDS-PAGE gels and transferred to a PVDF Immobilon-FL membrane (EMD Millipore, Catalog # IPFL00010). The PVDF membrane was incubated with blocking buffer (Rockland ...
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bioRxiv - Pathology 2023Quote: ... 20 μg total protein extract or of WGA-enriched proteins were separated using 4-15% SDS–PAGE gels and were transferred to nitrocellulose membrane (Millipore, Bedford, MA, USA). Blots were probed with primary antibodies and then developed with horseradish peroxidase (HRP)-enhanced chemiluminescence (WesternBright ECL ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and transferred onto polyvinylidene difluoride (PVDF) membrane (Immobilon-P Membrane, Millipore, Hertfordshire, UK) using standard techniques ...
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bioRxiv - Microbiology 2021Quote: ... transferred to the PVDF membrane (Immobilon PVDF membrane, Millipore Sigma, Burlington, MA, USA), and immunoblotted with BCL-2 ...
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bioRxiv - Plant Biology 2023Quote: ... Membrane transfer was done according to manufacturer’s instructions onto Immobilon PVDF membrane (Millipore). Blocking was performed using 5% milk in PBS with 0.1% Tween (PBST) ...
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bioRxiv - Physiology 2024Quote: ... for SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon Transfer Membrane, Millipore) and then blocked in Tris-buffered saline (TBS)-Tween 20 containing 2% milk protein for 30 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... This new pellet was resuspended in CellVue Claret membrane kit-staining-component Diluent C (Sigma-Aldrich, Miniclaret-1kt). Staining of membranes took place according to manufacturer’s instructions and was stopped by adding 2% bovine serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... are stained for 5 minutes at room temperature RT with 0.5μl of PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling (#P7333, Sigma) and then polished with the exosome spin columns (#4484449 ...
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bioRxiv - Microbiology 2023Quote: ... stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane (Sigma-Aldrich, Saint Louis, Missouri, USA) according to manufacturer’s instructions and added at a concentration of 5×105 monocytes per model the day before adding epithelial cells.
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...