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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) for 20-24h at 20°C ...
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bioRxiv - Cell Biology 2021Quote: ... eosinophil peroxidase (EPX) and Fibroblast specific protein-1 (FSP-1) were procured from Sigma-Aldrich (Merk). All other important reagents were procured from sigma other than specified Sigma-Aldrich (Merk).
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bioRxiv - Molecular Biology 2024Quote: ... the protein expression was induced by 1 mM isopropyl 1-thio-β-D-galactopyranoside (IPTG, Sigma) at 16°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 1% BSA at 4 °C overnight: rabbit anti-microtubule-associated protein 2 (MAP2, Millipore 1: 200); sheep polyclonal anti-dopamine beta hydroxylase (DBH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... In the first experiment, rats were injected with either sterile saline (vehicle; 1 mL/kg, i.p.) or propranolol hydrochloride (PROP, Sigma-Aldrich, St Louis ...
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bioRxiv - Neuroscience 2019Quote: ... Western blotting was performed using an antibody directed towards rat kisspeptin (1:500; EMD Millipore AB9754; Billerica, MA; Table 2). This antibody has been extensively characterized (Desroziers et al. ...
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bioRxiv - Neuroscience 2021Quote: ... In three rats brain stem sections were first immunoreacted with a goat anti-choline acetyltransferase antibody (1:1000; Millipore Sigma) and a rabbit anti-goat IgG conjugated to horseradish peroxidase (Vector Labs) ...
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bioRxiv - Developmental Biology 2020Quote: ... cryosections were incubated overnight at 4°C with the following primary antibodies: rat monoclonal anti-Neural Cell Adhesion Molecule L1 (1:500 Millipore, Cat # MAB5272 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C in blocking buffer with the following primary antibodies: rat anti-MBP (monoclonal, 1:100, Millipore), rabbit anti-Parvalbumin (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and APC rat anti-mouse 4D4 (marking resident microglia; 1:1000) in blocking buffer containing 0.2% bovine serum albumin (BSA, Sigma-Aldrich) in HBSS ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then resolved by SDS-PAGE and western blotting was carried out as described in (Hansen et al., 2014) using the following antibodies: DAT (rat MAB369, 1:1000, Millipore) or HA (rat 3F10 ...
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bioRxiv - Immunology 2021Quote: ... Freshly isolated cells (4 x 106) were seeded in a pre-prepared gel matrix containing 1 mg/mL rat tail collagen type I (Merck Millipore) in DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... siRNA reverse transfected cells were grown to confluency before overlaying with neutralised type I collagen (1 mg/ml; Rat tail; Millipore). Collagen was allowed to set for 45 minutes before overlaying with complete growth media ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed in TBST and blocked again with BSA followed by incubation with anti-rat IgG conjugated with horseradish peroxidase (dilution 1:20000, cat. no. A5795, Sigma) in TBSTB for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... followed by TBS-Tx (2 × 5 min) and incubated with primary antibody (Rat anti-muscarinic M2 monoclonal antibody, unconjugated, clone m2-2-b3, 1:750, Millipore Cat ...
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bioRxiv - Plant Biology 2024Quote: ... and labelled with the secondary antibody (diluted 1:50 in 0.1% BSA in PBS, Anti-Rat-IgG – Gold antibody; Sigma, USA) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by the addition of 50 µl of a 1 µg/ml rat anti-TREM2 detection antibody (#MABN2321, Sigma Aldrich) into the wells ...
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bioRxiv - Cell Biology 2019Quote: ... Their relative expression was monitored by ELISA using anti-FLAG-HRP (Sigma) or anti-HA-HRP antibodies (Roche Diagnostics) ...
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bioRxiv - Biochemistry 2021Quote: ... or alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for Elisa (Sigma P7998) were added as a substrate ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated overnight with anti-human IgG (Sigma Aldrich I5260) at 1:5 000 in PBS ...
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bioRxiv - Physiology 2024Quote: ... Serum insulin was measured by commercial ELISA (EMD Millipore cat. EZRMI-13K).
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bioRxiv - Cell Biology 2021Quote: ... nonfat dry milk in PBS-T for 1 h Membranes were incubated with mouse anti-FLAG M2 antibody (1:500, Sigma-Aldrich) or mouse anti-α-tubulin B512 antibody (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated with 1:10,000 vol vol−1 secondary antibody anti-mouse horseradish peroxidase (HRP) conjugate (A5278, Sigma–Aldrich, USA) in 2% weight vol−1 BSA-TTBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was blocked for 1 h with 5 % milk in TBST and blotted for anti-ssDNA (1:1000, Millipore, MAB3034).
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bioRxiv - Neuroscience 2022Quote: ... and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET), labeling mixture that was incubated for 10 min at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were exposed to either fluorophore-conjugated secondary antibodies (Li-Cor; 1:2000) or HRP-conjugated secondary antibodies (Sigma; 1:3000) in TBST with 5% milk for 30 min (Li-Cor ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was blocked with 5% BSA and incubated with primary antibodies against Tuj-1 (dilution 1:10,000, Sigma, Catalog # T8578) and pan-Ago (dilution 1:1200 ...
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bioRxiv - Neuroscience 2021Quote: ... Anti HA rat monoclonal antibody was purchased from Sigma-Roche (clone 3F10 ...
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bioRxiv - Cell Biology 2021Quote: ... coated with 50 µg/mL rat tail collagen (Sigma). Inlets of the microfluidic channels were connected with tubing to syringes filled with complete media in a syringe pump (Harvard Apparatus) ...
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bioRxiv - Biophysics 2021Quote: ... and anti-HA high affinity from rat (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... rats self-administered methamphetamine hydrochloride (Sigma, St Louis, MO). The exception is when sucrose was used as the reinforcer ...
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bioRxiv - Neuroscience 2020Quote: ... rats were transcardially perfused with paraformaldehyde (PFA, Sigma-Aldrich) containing 0.2% picric acid (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... BM cells were blocked with purified Rat IgG (Sigma) and stained with an antibody cocktail in SM containing CD3 PE/Cy5 (eBioscience ...
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bioRxiv - Plant Biology 2021Quote: ... at 37° C in FITC anti-rat IgG (Sigma) diluted 1:40 in PBS containing 2% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... and rat collagen type I were purchased from Sigma. According to Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... All samples were pretreated with rat serum (Sigma-Aldrich) and incubated with anti-mouse/human CD207 PE (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... and subsequently blocked with purified rat IgG (Sigma-Aldrich). Cells were then stained with c-Kit-APC-Cy7 (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... coated with rat-tail collagen I (Sigma, Cat # C3867). For creating a gradient ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rpb1-S7-P clone 3E12 (rat, monoclonal, Millipore), anti-Cdc28 clone yC-20 (goat ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rpb1-S5-P clone 3E8 (rat, monoclonal, Millipore), anti-Rpb1-S7-P clone 3E12 (rat ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rpb1-S2-P clone 3E10 (rat, monoclonal, Millipore), anti-Rpb1-S5-P clone H14 (mouse ...
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bioRxiv - Genetics 2019Quote: ... and FITC-conjugated rabbit anti-rat IgG (Sigma, # F1763), both 1:600 in blocking buffer] for 3 h at 30°C ...
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bioRxiv - Systems Biology 2019Quote: ... with type I collagen from rat tail (Sigma-Aldrich) by incubating plates with 65 microliter of 4mg/ml collagen I solution in PBS for two hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-PfCentrin3 and Rat anti-HA 3F10 (Sigma) were diluted 1:250 in 1.2 ml 3% BSA/PBS ...