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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Immunology 2019Quote: ... for 6 hours and then exposed to 0.5 × 105 red and 0.5 × 105 blue opsinised (1 hour in 10%v/v human serum) 3 μm diameter latex polystyrene beads (Sigma-Aldrich, Gilligham, UK) such that there were 1 bead per cell ...
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bioRxiv - Immunology 2020Quote: ... human TLR4/CD14/MD2 or non-transfected (null-1) were cultured and maintained using DMEM GlutaMAX™ containing 10 % FBS (Sigma-Aldrich), 1 % P/S ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 2 mM L-glutamine and 1% Antibiotic -Antimycotic (Life Tech, UK) with 20 ng/mL human epidermal growth factor (hEGF, Sigma-Aldrich, UK), 20 ng/mL human basic fibroblast growth factor (hbFGF ...
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bioRxiv - Immunology 2020Quote: Cells were stained directly without fixation in FACS buffer (PBS supplemented to 1% FBS, 10 μg/mL human-IgG (Sigma, #I8640-100MG), and 0.01% Sodium azide ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of anti-human horseradish peroxidase (HRP)-conjugated secondary antibodies for IgG detection (diluted 1:50000, Sigma-Aldrich, A0170) to each well for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Primary human monocyte-derived macrophages (hMDMs) were seeded at 1×105 cells per well in RPMI-1640 supplemented with human serum (10% v/v) (Sigma, cat# H4522) for 16 hours prior to infection ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and developed by detecting bound IgG or IgA with appropriate fluorochrome-labelled secondary antibodies: 1 µg/ml goat anti-human IgG-Cy3 (Sigma, Cat# C2571) or goat anti-human IgA-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2024Quote: Phagocytosis capacity was assessed for 500’000 PBMCs per well in a 24-well plate in 500µl medium (RPMI with 10% FBS, 1% penicillin-streptomycin) supplemented with 20% of human AB serum (Sigma-Aldrich Cat.# H3667). PHrodo E ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CFBE-F508del-CFTR or CFBE-WT-CFTR were seeded at 140.000 cells/well in a FNC-coated 24-well plate for a day and then incubated for 24 h with ascending concentrations of correctors diluted in growth medium deprived of FBS but supplemented with 1% Human Serum Albumin (HSA, Sigma-Aldrich, #A1653). The same protocol was used for treatment with proteasome inhibitor MG-132 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 or PC-3 cells were transduced with virus-containing culture media supplemented with 10 µg/ml Polybrene (Millipore, Cat #TR-1003-G) for 24 hours and replaced with fresh growth media ...
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bioRxiv - Microbiology 2022Quote: ... variant was obtained from the RIVM (Netherlands) through European Virus Archive global and expanded on VeroE6 cells grown in DMEM (Sigma Aldrich, Cat #D6429) supplemented with 4% of FCS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and 100 μL per well virus solution with or without 1μg/mL TPCK-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA, Cat# 4352157) was added per well ...
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bioRxiv - Cell Biology 2024Quote: One rabbit was immunized with 500 μg of recombinant arpin by subcutaneous injection of an emulsified 1:1 mixture of protein and TiterMax Gold (Sigma Aldrich; St. Louis, MO). Three more immunizations were performed 14 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Biochemistry 2021Quote: ... 30 KDa for T-protein and L-protein and 100 KDa for P-protein) were purchased from Millipore (Billerica, MA, USA). Mut Express II Fast Mutagenesis kit V2 was purchased from Vazyme (Jiangsu ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 1.25 mg/ml human insulin (Sigma #I9278), 1 % Penicillin-Streptomycin (Sigma #P4333 ...
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bioRxiv - Neuroscience 2022Quote: ... human basic fibroblast growth factor (bFGF) (Sigma, cat. #F0291), HEPES 1M (Life Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Normal Human Dermal Fibroblasts (NHDFs) (Sigma-Alrdrich, Buchs, Switzerland), a non-cancerous human skin fibroblast cell line ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10 % heat-inactivated pooled human serum (Sigma) and 100 ng/ml macrophage colony stimulating factor (R&D systems) ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgG (Fab specific) – peroxidase-conjugated antibody (Sigma) diluted 1:5000 in blocking buffer was added to the wells and incubated for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... human Collagen IV from Sigma-Aldrich (St. Louis, MO), recombinant human Tenascin C ...
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bioRxiv - Cell Biology 2020Quote: ... or shRNA directed against human IP6K1 (TRC0000013508, Sigma-Aldrich) were co-transfected with VSV-G and psPAX2 (a gift from Didier Trono ...
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bioRxiv - Bioengineering 2019Quote: ... Human laminin (pepsinized; Millipore Sigma, Burlington, MA, Catalog #AG56P), human tenascin-C (Millipore Sigma ...
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bioRxiv - Immunology 2019Quote: ... with 5% human serum (Sigma-Aldrich; H5667 or H4522), 1% penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... were incubated with 25% human immune serum (Sigma-Aldrich) for 30 min ...
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bioRxiv - Bioengineering 2019Quote: Human hepatic stellate cells (LX-2s [34], Millipore Sigma) were used between passages 6-8 for all experiments ...
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bioRxiv - Microbiology 2021Quote: Human neuronal progenitor cells (EnStem-A™ SCC003, Millipore) were cultured on plates previously cultured with geltrex (A1413201 ...
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bioRxiv - Immunology 2020Quote: ... with 5% human serum (Sigma-Aldrich; H5667 or H4522), 1% penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in human serum from male AB plasma (Sigma) and incubated for 16 h at 37 □C with shaking (180 rpm) ...
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bioRxiv - Neuroscience 2021Quote: ... human leukemia inhibitory factor (hLIF, 10 ng/ml, Millipore), SB431542 (2 mM) ...
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bioRxiv - Biophysics 2020Quote: ... ErbB2 (Her2) human recombinant was purchased from Sigma-Aldrich. Deglycosylated trastuzumab was obtained by treatment with Endo S (New England BioLabs ...
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bioRxiv - Cell Biology 2021Quote: ... HT medium containing 20 nM human EGF (Sigma-Aldrich) was added to the cells at a final concentration of 10 nM (1 ml of 20 nM EGF in HT medium was added to the glass-bottom dish containing 1 ml HT medium) ...