Labshake search
Citations for Millipore Sigma :
2351 - 2400 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... Cells were released from G1 by washing with YPD containing auxin and 0.15 mg/mL pronase E (Sigma Aldrich) three times ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were incubated with 2 ml Williams E media containing the indicated concentrations of oleic acid (Sigma 05508-5ML), 1 μg/ml Brefeldin A (Sigma B6542-5MG ...
-
bioRxiv - Neuroscience 2021Quote: ... 2-(2-Furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine (SCH 58261, 50nM) were purchased from Sigma (St. Louis, MO). CGS 21680 and SCH 58261 were made up into a 5 mM stock solution in dimethylsulfoxide (DMSO) ...
-
bioRxiv - Genomics 2022Quote: ... in TC-treated 6-well plates coated with 0.05 mg/mL Poly-D-Lysine (Sigma Aldrich A-003-E).
-
bioRxiv - Biochemistry 2022Quote: ... 0.25 mg/ml Pepstatin A and 0.25 mg/ml E-64) or Roche cOmpleteTM EDTA-free protease inhibitor cocktail tablet (Millipore-Sigma). NP-40 (final concentration of 0.5% ...
-
bioRxiv - Biophysics 2022Quote: ... E-cadherin knockdown was induced in shE-cadherin cells by adding 200 μM isopropyl-β-D-thiogalactoside (IPTG; Sigma) 72 h prior to experiments.
-
bioRxiv - Immunology 2020Quote: ... Cells were plated in 96-well plate in density of 1×106/mL in 200μL of medium and cultured at 37°C and 5% CO2 for 48h in presence of 5μg of lipopolysaccharide (LPS, isolated from E. coli strain 055:B5, Sigma, St ...
-
bioRxiv - Genetics 2022Quote: ... cells were washed three times in YPD (30°C) containing 0.1 mg/ml Pronase E (Sigma-Aldrich; Catalogue # P5147), resuspended in SC minimal medium containing nocodazole (15μg/ml) ...
-
bioRxiv - Cancer Biology 2022Quote: Sirt1 conditional knockout E-NOTCH1-induced T-ALL-bearing mice were treated with vehicle only (corn oil; Sigma, C8267) or tamoxifen (Sigma ...
-
bioRxiv - Bioengineering 2019Quote: ... Ice-cold acetone-fixed cryostat sections (10μm) were prepared and stained with hematoxylin and eosin(H&E) (Sigma-Aldrich) using routine methods ...
-
bioRxiv - Genetics 2020Quote: ... Cell lysates were supplemented with IPH50 buffer and protease inhibitor cocktail (AEBSF, 1,10-Phenanthroline, Pepstatin A, E-64) (Sigma), centrifuged at 15,000rpm for 20 minutes ...
-
bioRxiv - Plant Biology 2021Quote: Around 750 entire-4 (e-4) seeds were treated with the mutagenic substance Ethyl-Methane Sulfonate (EMS, Sigma m0880) at a concentration of 0.6% for 10 hours ...
-
bioRxiv - Biophysics 2021Quote: The ATPase activity of MutL or MutL(R-E) was measured by an ATPase/GTPase Activity Assay Kit (Sigma). The analysis was carried out with 5 μM protein in a 40 μL reaction mixture comprised of 20 mM Tris-HCl (pH 7.8) ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were collected by filtration and promptly washed twice on the filter using one culture volume of H2O and then resuspended into YPD medium containing 0.2 mg/mL pronase E (Sigma).
-
bioRxiv - Bioengineering 2022Quote: ... samples were stained with hematoxylin and eosin (H&E) through a 5- minute incubation in Harris Hematoxylin (Sigma Aldrich), followed by a wash in tap water and three dips in acid ethanol to destain ...
-
Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... These sections were stained with Hematoxylin & Eosin (H&E) and periodic acid-Schiff (PAS) according to the manufacturer’s protocol (Sigma). The muscle sections were analyzed blindly by a pathologist ...
-
bioRxiv - Neuroscience 2023Quote: ... The final concentrations of drugs used in the study: 10 μM of E-64 (Sigma-Aldrich, Cat.: #E3132-1MG), 60 nM of t-NED19 (Bio-techne ...
-
bioRxiv - Biochemistry 2023Quote: ... The beads were washed three times with buffer E and then mixed with 60 μg of 3xFLAG peptide (SIGMA) in 60 μl of buffer E for 1 h with shaking at 1400 rpm at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... 13 weeks old mice were injected intraperitoneally (i.p.) with 5□mg/kg of LPS (L2880, E. coli O55:B5, Sigma) or endotoxin-free PBS between 1 p.m ...
-
bioRxiv - Bioengineering 2024Quote: ... 1L of perfusate was composed of 950 mL of William’s E hepatocyte culture media (Sigma Aldrich, Burlington, MA, USA) to supply the graft with amino acids and nutrition during perfusion ...
-
bioRxiv - Immunology 2023Quote: Camostat (cat. no. SML0057) and E-64d (cat. no. E8640) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A short synthetic dsRNA ...
-
bioRxiv - Developmental Biology 2024Quote: ... for 1 min 30 seconds at high intensity in a 200 mTorr vacuum and then incubated at room temperature for 1h with 200µL/well of 200 µg/mL Poly-D-Lysine (A-003-E, Merck Millipore) diluted in 0.1M HEPES pH 8.4 (H3784-25G ...
-
bioRxiv - Neuroscience 2024Quote: ... Maternal Inflammatory Response (MIR) was induced with low-dose lipopolysaccharide (LPS, E. coli serotype 0111:B4; 0.008 mg/kg, Sigma) administered in a single I.P ...
-
bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
-
bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
-
bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with either HRP-linked goat anti-mouse IgG antibody (dilution 1:10,000; Sigma-Aldrich) or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were grown to 70% confluency before induction of protein expression using 1 μg/ml tetracycline (Sigma), and media supplementation with 50 μM biotin for protein labeling ...
-
bioRxiv - Cell Biology 2020Quote: ... expression of EGFP fusion proteins was induced by addition of 1 μg/mL doxycycline (Sigma-Aldrich, D9891) and EGFP expressing cells were positively selected by FACS using a SONY MA900 to sort into media lacking doxycycline ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 mg protein was incubated with 50 μl equilibrated anti-FLAG M2 affinity gel (Sigma–Aldrich, A2220) on a rotating wheel for 1 h at 4 °C ...
-
bioRxiv - Physiology 2019Quote: ... GST-free eluates of protein were prepared by using 1 unit of biotinylated thrombin (Millipore 69672-3) per 100 μl of beads and letting samples incubate on a rotator at 4° C overnight ...
-
bioRxiv - Biochemistry 2019Quote: ... the buffer matrix for the protein standards was changed by adding 1% BSA (A7030, Sigma Life Science) to achieve a higher matrix complexity ...
-
bioRxiv - Cell Biology 2021Quote: ... Mif2-TAF and Flag-Cse4 were detected by immunoblotting with anti-protein A (Sigma, P3775, 1:10,000) and anti-Flag (Sigma ...
-
bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were transfer to PVDF membranes and detected using either monoclonal anti-GFP (1:1000; Sigma, 11814460001), anti-HA 12CA5 (1:1000 ...
-
bioRxiv - Systems Biology 2021Quote: ... at a 1:50 protease/protein ratio overnight at 37 °C in 100 mM NH4HCO3 (Sigma-Aldrich). The next day ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Total protein synthesis was measured by immunoblot using the anti-puromycin antibody at 1:500 (EMD Millipore). Protein synthesis was quantified by densitometry using ImageJ and normalized by immunoblot using antibody specific to steady-state levels of eIF2α protein (13).
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were separated by SDS-PAGE and probed with antibodies against zDHHC5 (1:1,000; Sigma Prestige, HPA014670), HA (1:1,000 ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were then detected with monoclonal mouse anti-FLAG M2 (1:1000; Sigma-Aldrich, St. Louis, MO) and polyclonal rabbit anti-6xHis (1:2,000 ...
-
bioRxiv - Microbiology 2020Quote: ... HA3-tagged proteins were detected using a monoclonal rat α-HA3 (1:1000; clone 3F10; Sigma-Aldrich). Actin ...
-
bioRxiv - Microbiology 2022Quote: ... and the recombinant protein expression was induced with 0.5 mM isopropyl-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich). The bacteria were incubated at 18°C for additional 18 hours ...
-
bioRxiv - Cell Biology 2022Quote: Flag-tagged proteins were captured from 1 mg total cell lysate using anti-Flag affinity matrix (Sigma) for 1 hour at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
-
bioRxiv - Immunology 2022Quote: ... All lentiviral vectors (pLKO.1) expressing shRNAs used for knockdown of host proteins were purchased from Sigma.
-
bioRxiv - Cancer Biology 2019Quote: ... The lyophilized proteins were resuspended in 1 ml of a 2 mg/ml DTT (1,4-Dithiothreitol, Sigma) solution and incubated at 50C for 2h ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 mg of extracted protein in lysis buffer was immunoprecipitated overnight with Flag-M2 affinity gel (Sigma) at 4°C ...
-
bioRxiv - Systems Biology 2021Quote: ... Samples were digested overnight at 37°C using trypsin (1:100 w/w, enzyme/protein, Sigma-Aldrich) and LysC (1:100 w/w ...
-
bioRxiv - Developmental Biology 2019Quote: ... The target protein levels were normalized to the endogenous control (GAPDH, mouse, Millipore Sigma, MAB374, 1/500). The membranes were scanned on the LI-COR Odyssey CLx imaging system.
-
bioRxiv - Genetics 2020Quote: ... Equal protein loading was confirmed by probing with mouse-anti-β-actin antibody (1:10,000; Sigma, A5441).