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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... followed by nucleic acid digestion with 0.5 Units of Benzonase per 1 µg of protein (Sigma-Aldrich) for 30 min at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... supernatant protein was removed by acidification with 5 g L-1 trichloroacetic acid (Supelco from Sigma-Aldrich), incubation for 20 min in room temperature and centrifugation at 20,238 x g for 10 min in a table-top centrifuge (Eppendorf 5424 ...
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bioRxiv - Biophysics 2022Quote: ... the expression of the protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG – Sigma) and the flask was placed at 37°C ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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bioRxiv - Neuroscience 2023Quote: ... or growth associated protein-43 (GAP43; rabbit anti-GAP43; 1:1000; #AB5220, AB_2107282; Millipore, Billerica, MA, USA). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Developmental Biology 2024Quote: ... TBX33xFLAG proteins were detected by chemo-luminescence using monoclonal M2 anti-FLAG antibodies (1:500, Sigma, F1804) in combination with donkey anti-mouse IgG-HRP antibodies (1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were detected by western blotting using the following antibodies: Anti-GFP antibody (1:3000 dilution; Millipore), anti-aldolase antibody (1:3000 ...
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bioRxiv - Microbiology 2023Quote: ... 1:200) in the presence of protein A-sepharose beads (20 µl of 50% bead solution, Sigma) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Biochemistry 2022Quote: ... Collected protein was incubated 1 hour with 200 µL ANTI-FLAG-M2 affinity resin (Sigma-Aldrich; A2220) pre-washed with 3 times with1.0 mL Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using commercially available antibodies: anti-Flag mouse Antibody M2 (1:1000, Sigma-Aldrich M8823) and rabbit anti 4R-tau Antibody (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA was digested by incubation with benzonase (Sigma, 25 U per expected 1 mg of protein) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: The NSP3(1-110) protein was then immobilized on Anti-Flag M2 Affinity gel (Sigma Aldrich, A2220), and the cell lysates from stable cell line 293T-N ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to nitrocellulose membrane for immunodetection using mouse anti-puromycin (1:1000,12D10, MABE943 Sigma) and mouse anti-actin (1:25 ...
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bioRxiv - Molecular Biology 2024Quote: ... and in N2B27 serum-free media containing Mitogen-Activated Protein Kinase (MEK) inhibitor PD0325901 (1 μM) (Millipore), Glycogen Synthase Kinase 3 (GSK3 ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Immunology 2023Quote: ... and immunoprecipitated with protein A and protein G sepharose beads (Millipore Sigma). After thoroughly washing beads with SLBA buffer (0.15M NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Tau protein ladder (Sigma) was used in some experiments as a control ...
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bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
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bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
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bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein disulfide isomerase (Sigma) was used in positive control reactions and albumin (Research Organics ...
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bioRxiv - Immunology 2021Quote: ... plus protein A (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... S protein agarose (Millipore) was added to each sample ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Protein standard (Sigma: P5369) was used as standard (ranging from 0-0.5mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...
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bioRxiv - Immunology 2024Quote: ... protein G beads (Millipore) were used (2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Protein-A agarose (Sigma), Streptavidin agarose (ThermoFisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were blocked for 30 minutes with mouse serum and immunostained with directly Cy3-conjugated anti-human HNA (Millipore, MAB1281C3, 1:100). Nuclei were counterstained using DAPI ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we grew PAO1-pvdSi in iron-limited CAA medium (using 100 mg ml−1 human-apo transferrin to bind iron and 20mM NaHCO3 as cofactor, both from Sigma-Aldrich, Switzerland), supplemented with various amounts of purified pyoverdine (pyoverdine concentration in μM ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were washed thrice for 15 min in PBSTx and incubated for 24 hrs in 1:2000 Fab specific FITC conjugated goat anti human IgG (F5512, Sigma-Aldrich UK) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for immunostaining include rabbit anti-human/mouse PKD-1 (4502371) and mouse anti-mouse α-smooth muscle actin(A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...