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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 5 µg/mL human insulin (Sigma Cat. #91077C), 0.1 nM cholera toxin (Sigma ...
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bioRxiv - Genomics 2024Quote: ... + 5% Human Serum (Sigma-Aldrich catalogue number H4522) + 1% Penicillin-Streptomycin (Gibco catalogue number 15140122 ...
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bioRxiv - Immunology 2024Quote: ... an added positive human IgG Kappa (Sigma-Aldrich) loaded control lane was included (lane P) ...
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bioRxiv - Immunology 2024Quote: ... and 5% human heat-inactivated AB serum (Sigma). The antibodies used for stimulation were anti-human CD3 (clone UCHT1 ...
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bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Neuroscience 2019Quote: ... plane through the human temporo-basal neocortex and post-fixed for 30 to 60 min in 0.5 or 1% osmium tetroxide (Sigma, Munich, Germany) diluted in PB-buffered sucrose (300 mOsm ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Females were superovulated at 6-8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Bioengineering 2021Quote: ... or type I collagen microchannels coated overnight with 50 μg mL−1 human placental collagen IV (Cat. no. C5533, Sigma-Aldrich) and 25 μg mL−1 fibronectin from human plasma (Cat ...
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bioRxiv - Immunology 2021Quote: ... SARS2-16 (hybridoma supernatant diluted 1:6,000 to a final concentration of ∼20 ng/mL) and then HRP-conjugated goat anti-human IgG (Sigma-Aldrich, A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Developmental Biology 2022Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Immunofluorescence was performed using an automatic liquid handling dispenser (Personal Pipetter, ApricotDesigns) using Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543) and Chicken anti-human MAP2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... were seeded at 15,000 cells.cm−2 in 96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (100ng.mL−1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell ...
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bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
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bioRxiv - Bioengineering 2024Quote: ... and Endothelin-1) using a fluorometric MILLIPLEX ® Human Angiogenesis/Growth Factor Magnetic Bead Panel Multiplex Assay (Sigma-Aldrich, Cat # HAGP1MAG). Each plate was run according to the manufacturer’s protocol using 25 µL sample per well.
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bioRxiv - Microbiology 2022Quote: ... Samples of gB-SE or gD-SE immobilized on Strep-Tactin Sepharose beads were incubated with 1 mL of diluted human γ-globulins (G4386; Sigma) (0.082 and 3.04 mg/mL in medium 199 containing 1% FCS and ADCC assay buffer for neutralization assays and ADCC assays ...
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Molecular Biology 2023Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Immunology 2024Quote: ... were adjusted to 5 x 106 bacteria per 50 μL in PBS then treated with indicated OspC MAbs (1, 2, 3, 10, 20, 30 μg/mL) in the presence or absence of 20% human complement (Sigma-Aldrich) for 16 or 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
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bioRxiv - Bioengineering 2021Quote: Cells were cultured in the appropriate culture media and infected with S-pseudotyped virus in the presence of polybrene (Sigma; TR-1003) to a final concentration of 5 µg/ml.
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL of each purified virus library were incubated with 100 μL of an endonuclease cocktail consisting of 1000U/mL Turbonuclease (Sigma T4330-50KU) with 1X DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Microbiology 2022Quote: ... we concentrated ∼75 mL of virus to ∼3 mL (25x concentration) using an Amicon Ultra-15 Centrifugal Filter Unit (Millipore Sigma; UFC905008). All viral and lentiviral infections and transductions were done in the presence of 20 μg/mL DEAE-Dextran (Sigma ...
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bioRxiv - Genetics 2024Quote: ... The recipient cells were infected 48 hours post-transfection of HEK 293 cells with the virus supernatant in the presence of 8 μg/ml polybrene according to the manufacturer’s guidelines (Sigma-Aldrich, MO, USA). The cells transduced with the pLVX-Tight-Puro-FoxM1-dNdK were selected in 2 μg/ml puromycin (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... were spin transduced with 10-20μl of the concentrated virus at 37°C at 2200g for 90 minutes with polybrene reagent (8-10μg/ml, Millipore Sigma, Billerica, MA). The cells were washed with PBS and resuspended in fresh RPMI media supplemented with 10% FBS with 1% penicillin and streptomycin 24 hours post-transduction ...
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bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...
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bioRxiv - Cell Biology 2023Quote: ... lentiviral expression plasmids were co-transfected with ΔNRF and VSVG (gifts from J. bear) virus packaging plasmids into HEK293T cells using 50 µg/mL polyethylenimine (PEI)-Max (Sigma-Aldrich Chemistry). Two days later ...
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bioRxiv - Immunology 2024Quote: ... Concentrated virus particles solubilized in IMDM were applied to HL60 cells in the presence of 8 μg/ml polybrene (Sigma-Aldrich #H9268). Clones expressing sgRNAs were selected with 2 μg/ml puromycin ...
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bioRxiv - Cell Biology 2024Quote: 3T3-L1 cells were grown in 6-well plates until 50-60% confluency before the medium was replaced by the concentrated virus mixture supplemented with 8 µg/mL polybrene (Sigma-Aldrich, #107689). After overnight incubation the medium was replaced by DMEM supplemented with 10% CS ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... For the time course shown in Fig 2B 19 μl of this protein were combined with 1 μl of trypsin (Sigma; 1 mg/ml) and the mixture was incubated at RT ...
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bioRxiv - Systems Biology 2020Quote: ... on day 0 with a 50 µl volume of Escherichia coli O111:B4 LPS purified by ion exchange and with <1% protein and <1% RNA (Sigma Aldrich; catalog L3024), which was diluted in sterile ...
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bioRxiv - Plant Biology 2022Quote: ... 20 mM Tris–HCl [pH 8.0], 5 mM imidazole, 5% glycerol, 1 mM PMSF, 1/10 protein inhibitor cocktail: Sigma, St. Louis, MO, USA). After centrifugation for 40 min at 21,000 xg ...
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bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Pathology 2023Quote: ... adiponectin levels were measured by enzyme-linked immunosorbent assays (Human Chemerin ELISA kit, Biovendor, Brno, Czech Republic; Human Resistin ELISA kit, Millipore Sigma; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
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bioRxiv - Immunology 2021Quote: ... The same volumes of PBS and LPS (20 μg/mouse, from E. coli O114:B4, Sigma-Aldrich, Shanghai, China) were used as negative control and positive control ...
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bioRxiv - Developmental Biology 2021Quote: ... 250–1000 embryos were collected and transferred into a Petridish containing Pronase E protease solution (Sigma-Aldrich, P5147-1G). When 20–30% embryos were hatched ...
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bioRxiv - Neuroscience 2021Quote: Cre-mediated recombination was induced via intraperitoneal injection of 4-hydroxytamoxifen (50:50 E and Z isomers, Sigma-Aldrich) dissolved in sunflower seed oil ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl cold lysisg/μl cold lysisl U6.3>Lhx5/Sox1/Six3 gRNA f+e was microinjected together with the Fast Green solution (Sigma) at a 2:1 ratio to ease the detection of the injection site of the developing neural tube and eye respectively with the help of borosilicate glass capillaries and electroporated as previously reported86–88 using the Intracel TSS20 OVODYNE Electroporator ...