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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Cell Biology 2020Quote: ... organoids were treated with 50μM FITC-conjugated deoxycholic acid (E. Mash, University of Arizona) or 40kDa FITC-conjugated dextran (Sigma, FD40S) (Jean-Louis et al ...
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bioRxiv - Neuroscience 2021Quote: ... Acute inflammatory-type macrophages were induced by treating cells with 10 ng/mL lipopolysaccharide (from E. coli 055:B5, Sigma) for 3.5 hours ...
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bioRxiv - Neuroscience 2019Quote: ... uterine horns of E 13.5 pregnant dams were exposed by midline laparotomy after anesthetization with Avertin (312 mg/kg, Sigma-Aldrich). 1 μl of DNA plasmid(s ...
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bioRxiv - Cell Biology 2019Quote: ... SBP-EGFP-E-cadherin was released from the ER by adding a final concentration of 40 µM D-Biotin (Sigma) in L-15 medium ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and e-cig cartridge aerosols were determined using 2’7’dichlorofluorescien diacetate (H2-DCF-DA) fluorogenic probe (Sigma-Aldrich Cat# 287810). H2-DCF-DA [5 mM] with NaOH [0.01N] was reacted for 30 minutes to prepare the dye ...
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bioRxiv - Biochemistry 2020Quote: ... ii) storage buffer (10 mM NaCl, 10 mM Tris-HCl) with 0.1 mg Pronase E from Streptomyces grisens (Sigma Aldrich) with 0.5% (v/v) ...
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently treated with 90nM cyst(e)inase in the presence of the following rescuing agents: 100uM Trolox (Sigma, 238813), 50nM ZVAD-FMK (SelleckChem ...
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bioRxiv - Cell Biology 2020Quote: ... 3d, e, Fig. 4b) or 15kPa gels (Fig. 4g, h) were treated with 15µM of Blebbistatin or DMSO (Sigma-Aldrich) in ENR medium for 3h ...
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bioRxiv - Neuroscience 2020Quote: ... vehicle (HBSS) or 100 ng/mL lipopolysaccharide (LPS E. coli O111:B4; 500,000 EU/mg; Sigma-Aldrich; St. Louis, MO) was administered for 3 hrs in Hx or Nx ...
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bioRxiv - Cell Biology 2021Quote: ... the imaging media with alpha factor was removed and new media with 0.1 mg/ml Pronase E (Sigma P-6911) was added to inactivate any remaining alpha factor ...
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bioRxiv - Biophysics 2022Quote: ... coli optimized gene sequence for expression of S.c. Cdc6 in E. coli (a generous gift of Dr. Megan Davey, Western University, Canada) was cloned into pET11 (Novagen). The Cdc6 gene was then subcloned into pGEX-6P-1 (GeneScript ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 weeks and 8 weeks old C57BL/6J mice were administered with E2 (Cat No. E-2758, Sigma, MO, USA), which was dissolved in ethanol and diluted in corn oil (Cat No ...
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bioRxiv - Biochemistry 2022Quote: ... 0.25 mg/ml Pepstatin A and 0.25 mg/ml E-64) or Roche cOmpleteTM EDTA-free protease inhibitor cocktail tablet (Millipore-Sigma). NP-40 (final concentration of 0.5% ...
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bioRxiv - Biochemistry 2022Quote: ... codon-optimized for Escherichia coli (E. coli) was cloned into the first multiple cloning site (MCS) of the pRSFDuet plasmid (Novagen), with N-terminal hexahistidine purification (His6 ...
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bioRxiv - Neuroscience 2021Quote: ... or glass coverslips in 24-well plates (for immunocytochemistry) coated with Poly-D-Lysine (5 μg/ml, PDL, A-003-E; Millipore) and laminin (1 mg/ml Sigma L2020) ...
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bioRxiv - Genetics 2019Quote: ... Cells were released from G1 by washing with YPD containing auxin (if indicated) and 0.15mg/mL pronase E (Sigma Aldrich) three times ...
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bioRxiv - Biochemistry 2019Quote: ... codon-optimized for Escherichia coli (E. coli) were cloned into the first multiple cloning site (MCS) of the pETDuet plasmid (Novagen), with N-terminal hexahistidine purification (His6 ...
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bioRxiv - Microbiology 2021Quote: ... was incubated in 50 mM Tris-acetate buffer (pH 7.4) containing 5 mg/mL protease mixture (Pronase E, Sigma-Aldrich) in a total volume of 1 mL at 28 °C for 2 h and then directly resuspended in the standard SDS sample buffer before SDS-PAGE and western blot analysis.
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bioRxiv - Neuroscience 2019Quote: hNSG and non-engrafted NSG mice (4-6 months old) were injected 3 mg/kg (i.p.) with lipopolysaccharide (LPS from E. coli; O55:B5, Sigma-Aldrich) or 0.9% saline solution ...
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bioRxiv - Biochemistry 2021Quote: ... 0.3 ml of media were precipitated with 25 µl EZ view Red anti-HA affinity gel (E 6779; Sigma-Aldrich) according to the manufacturers’ protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Seeds were surface-sterilized and sown in Petri dishes on half-strength Murashige and Skoog (MS/2) solid medium containing 0.8 % (w/v) agar-agar type E (Sigma Aldrich) and 1% sucrose ...
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bioRxiv - Molecular Biology 2020Quote: ... and calmodulin was ordered from Genewiz (codon optimized for expression in E. coli) and cloned into the NcoI and EcoRI sites of pET-28a (Novagen).
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bioRxiv - Immunology 2019Quote: ... pneumoniae in Colombia blood agar (E&O Laboratories, UK) followed by Todd-Hewitt broth plus 0·5% yeast extract (Sigma), both at 37°C in 5% CO2 ...
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Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-SeqbioRxiv - Developmental Biology 2021Quote: ... animal cap - (E) vegetal cap. Total RNA (X. laevis and A. mexicanum) was extracted using TriReagent extraction and LiCl precipitation (Sigma) (details in (Sindelka et al ...
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bioRxiv - Cell Biology 2022Quote: ... 80-mer oligonucleotides biotinylated either on the 5’ or 3’ end containing the E-box of the Lgr5 promoter or a mutated E-box (see table) were annealed with the corresponding antisense sequence and bound to streptavidin agarose beads (S1638, Millipore). Beads were washed 3 times ...
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bioRxiv - Neuroscience 2022Quote: ... and placed in a T75 flask (pre-coated with 50 μg/mL poly-D-lysine (Merck Millipore, A-003-E) for 1 hour) ...
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bioRxiv - Neuroscience 2022Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Genetics 2024Quote: ... in TC-treated 12- or 6well plates coated with 0.05 mg/mL Poly-D-lysine (Sigma-Aldrich A-003-E). At 3-4 DIV ...
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bioRxiv - Microbiology 2024Quote: ... The pesticides were: 3,6-dichloropyridine-2-carboxylic acid (i e. clopyralid, pesticide analytical standard, CAS 1702-17-6, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Cancer Biology 2024Quote: ... 80 μM aprotinin, 4 mM bestatin, 1.4 mM E-64, 2 mM leupeptin and 1.5 mM pepstatin A; Sigma-Aldrich, P8340) and 50 nM calyculin A (LC Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Neuroscience 2023Quote: ... and the gamma secretases inhibitor was purchased as Compound E (γ-Secretase Inhibitor XXI) from EMD Millipore (Burlington, MA, USA). Ammonium acetate (Cat# A11450 ...
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bioRxiv - Immunology 2023Quote: ... mice were injected intraperitoneally (i.p.) or intravenously (i.v.) with a single dose of LPS (E. coli O111:B4; Sigma Cat#L2630) 2.5 mg/kg ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were stained with hematoxylin and eosin (H&E) and for Tph1 with an anti-Tph1 primary antibody (Sigma; #T0678). Detection was performed using the polymer-based Envision FLEX+ detection system (Agilent).
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bioRxiv - Bioengineering 2022Quote: ... and tumor target (T) cells were co-cultured in triplicates at the indicated E/T ratio using flat bottom poly-L-ornithine (Sigma) coated 96-well plates with 1 × 104 target cells in a total volume of 200 µL per well in NALM-6 medium ...
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bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer-exchanged against HBS-E (10 mM HEPES at pH 7, 150 mM NaCl, 2 mM EDTA) by ultrafiltration using Amicon Ultra devices (Millipore).
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bioRxiv - Cell Biology 2023Quote: Transient knockdown of S100A11 and E-cadherin in MCF-10A cells was performed by transfection of MISSION® Predesigned (Sigma) or custom-made siRNAs ...
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bioRxiv - Bioengineering 2023Quote: ... and 5 μm sections were taken before being baked for 3 hours at 60°C and stained with hematoxylin and eosin (H&E, Sigma) or Masson’s trichrome (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... brain homogenate from a RML inoculated C57BL6/J mouse at the terminal phase of disease was treated with 100 μg/ml of pronase E (Sigma) for 30 min at 37 °C with shaking in a thermal mixer at 800 rpm ...
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bioRxiv - Immunology 2023Quote: ... HEK293FT (provided by T. Brdicka, IMG) and MC-38 cells (provided by E. Palmer, University Hospital Basel) were cultivated in DMEM media (Sigma). The media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: DO11.10 cell line (provided by Tomas Brdicka, IMG) and T2–Kb (provided by E. Palmer, University Hospital Basel) were cultivated in RPMI media (Sigma). HEK293FT (provided by T ...
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bioRxiv - Bioengineering 2023Quote: ... samples were mounted on paraffin blocks and sectioned into 5 µm sections then stained with hematoxylin and eosin (H&E, Sigma) or picrosirius red (PSR ...
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bioRxiv - Neuroscience 2024Quote: ... The first injection of pre-treatments was a solution of ethosuximide (150 mg/kg of 84 mg/ml in phosphate buffered saline, s.c.; Cat# E;7138, Sigma-Aldrich). Ethosuximide was used because the background strain ...
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bioRxiv - Immunology 2024Quote: ... The pool HIP-expressing bacteria was generated by transforming the purified pool plasmid into the Rosetta strain of E coli (Novagen). Four pool HIP-expressing Rosetta were combined to make a ‘super-pool’ ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... ii) 300 μg C g−1 13C-labelled Algal crude protein extract-98 atom% 13C (ISOTEC, Sigma-Aldrich), iii ...