Labshake search
Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5% human platelet lysate (Sigma #SCM152), w/o antibiotics and exchanged to the plated cell medium ...
-
bioRxiv - Biochemistry 2024Quote: Human serum albumin (Sigma-Aldrich, Lot No.: SLBZ2785) was reconstituted in 1 ml of PBS to achieve a final concentration of 120 mg/mL ...
-
bioRxiv - Neuroscience 2024Quote: ... 40% 1mg/mL collagen from human placenta (Sigma) and 10% fibronectin from bovine plasma (Sigma) ...
-
bioRxiv - Immunology 2024Quote: ... AP-conjugated anti-human IgG (A3187, Sigma-Aldrich) was used as a secondary at 1:2000 ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% AB human serum (Sigma-Aldrich), and plated onto non-treated tissue culture plates (Costar) ...
-
bioRxiv - Cancer Biology 2023Quote: Human recombinant IL-6 (Sigma-Aldrich, cat. I1395) was prepared as a 50 mg/mL stock solution in PBS/1%BSA ...
-
bioRxiv - Microbiology 2023Quote: ... the secondary antibody anti-human IgG-HRP (Sigma) was added at 1:1000 dilution ...
-
bioRxiv - Bioengineering 2024Quote: ... 0.01 mg/ml human recombinant insulin (Sigma, I9278), and 1% 100 mM sodium pyruvate solution ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 2 % human serum (HS, Sigma-Aldrich) at a concentration of 0.5×106 cells/ml ...
-
Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Heat-inactivated pooled human sera (Sigma-Aldrich, S1764) and rhesus sera were used as negative controls ...
-
bioRxiv - Cell Biology 2023Quote: ... 20ng/mL human fibroblast growth factor (Sigma F5392), B27 (Thermofisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Human skeletal muscle cells (HSMC, Sigma, #150-05F) were maintained in growth medium (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... 50 ng/mL Human Recombinant EGF (Sigma-Aldrich), 100 ng/mL Human Recombinant FGF-10 (Peprotech) ...
-
bioRxiv - Microbiology 2024Quote: ... medium supplemented with 4% human AB serum (Sigma), 2 mM L-glutamine (PAA) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5 μg/ml insulin solution human (Sigma-Aldrich) and 0.5 μg/ml hydrocortisone (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml recombinant human EGF (Sigma-Aldrich) and 0.5 μg/ml hydrocortisone ...
-
bioRxiv - Biophysics 2024Quote: ... Human Glu-fibrinopeptide (Sigma-Aldrich, St. Louis, MO) served as an internal standard and the peptide signal was recorded throughout all the analysis.
-
bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-human SFTPC (Millipore, ABC99, AR=TE), goat anti-Mrc1/Cd206 (R&D ...
-
bioRxiv - Genetics 2024Quote: ... 3% human serum (heat-inactivated from Sigma-Aldrich, St ...
-
bioRxiv - Biochemistry 2024Quote: ... and type V (human placenta; Sigma-Aldrich C3657) in aqueous buffer at pH 7.4 (200 mM sodium phosphate ...
-
bioRxiv - Microbiology 2024Quote: ... 20% v/v human serum (Sigma, cat# H4522) and penicillin/streptomycin at 37°C in the presence of 5% CO2 for 8 to 10 days ...
-
bioRxiv - Immunology 2023Quote: ... and enriched with 8% human serum (Sigma, H4522), 10 mM HEPES (Gibco ...
-
bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
-
bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
-
bioRxiv - Neuroscience 2019Quote: ... plane through the human temporo-basal neocortex and post-fixed for 30 to 60 min in 0.5 or 1% osmium tetroxide (Sigma, Munich, Germany) diluted in PB-buffered sucrose (300 mOsm ...
-
bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
-
bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2020Quote: ... Females were superovulated at 6-8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
-
bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
-
bioRxiv - Bioengineering 2021Quote: ... or type I collagen microchannels coated overnight with 50 μg mL−1 human placental collagen IV (Cat. no. C5533, Sigma-Aldrich) and 25 μg mL−1 fibronectin from human plasma (Cat ...
-
bioRxiv - Immunology 2021Quote: ... SARS2-16 (hybridoma supernatant diluted 1:6,000 to a final concentration of ∼20 ng/mL) and then HRP-conjugated goat anti-human IgG (Sigma-Aldrich, A6029) in PBS supplemented with 0.1% (w/v ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
-
bioRxiv - Developmental Biology 2022Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... Immunofluorescence was performed using an automatic liquid handling dispenser (Personal Pipetter, ApricotDesigns) using Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543) and Chicken anti-human MAP2 (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... were seeded at 15,000 cells.cm−2 in 96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (100ng.mL−1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell ...
-
bioRxiv - Microbiology 2022Quote: ... Samples of gB-SE or gD-SE immobilized on Strep-Tactin Sepharose beads were incubated with 1 mL of diluted human γ-globulins (G4386; Sigma) (0.082 and 3.04 mg/mL in medium 199 containing 1% FCS and ADCC assay buffer for neutralization assays and ADCC assays ...
-
bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
-
bioRxiv - Molecular Biology 2023Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... and Endothelin-1) using a fluorometric MILLIPLEX ® Human Angiogenesis/Growth Factor Magnetic Bead Panel Multiplex Assay (Sigma-Aldrich, Cat # HAGP1MAG). Each plate was run according to the manufacturer’s protocol using 25 µL sample per well.
-
bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
-
bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
-
bioRxiv - Bioengineering 2021Quote: Cells were cultured in the appropriate culture media and infected with S-pseudotyped virus in the presence of polybrene (Sigma; TR-1003) to a final concentration of 5 µg/ml.
-
bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
-
bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 5 μL of each purified virus library were incubated with 100 μL of an endonuclease cocktail consisting of 1000U/mL Turbonuclease (Sigma T4330-50KU) with 1X DNase I reaction buffer (NEB B0303S ...
-
bioRxiv - Microbiology 2022Quote: ... we concentrated ∼75 mL of virus to ∼3 mL (25x concentration) using an Amicon Ultra-15 Centrifugal Filter Unit (Millipore Sigma; UFC905008). All viral and lentiviral infections and transductions were done in the presence of 20 μg/mL DEAE-Dextran (Sigma ...
-
bioRxiv - Cancer Biology 2023Quote: ... were spin transduced with 10-20μl of the concentrated virus at 37°C at 2200g for 90 minutes with polybrene reagent (8-10μg/ml, Millipore Sigma, Billerica, MA). The cells were washed with PBS and resuspended in fresh RPMI media supplemented with 10% FBS with 1% penicillin and streptomycin 24 hours post-transduction ...
-
bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...