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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... we plated the cells on the 12 mm round coverslips coated with 1x Poly-D-Ornithine (Sigma-Aldrich) in 12 well plate ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with 50 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, Zwijndrecht, The Netherlands) and 1 µM ionomycin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... γδ T cells were stimulated with 30 ng/mL phorbol 12-myristate 13-acetate (PMA; P1585, Sigma-Aldrich) and 1 μg/mL ionomycin (I9657 ...
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bioRxiv - Physiology 2021Quote: ... 12 week-old mice were fed ad libitum and given CL316243 (3 mg/kg body weight; Sigma Aldrich) or vehicle (0.5% carboxymethylcellulose in sterilized water ...
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bioRxiv - Microbiology 2020Quote: ... U937 and THP1 cell lines were differentiated by treatment with phorbol-12-myristate-13-acetate (PMA, Sigma-Aldrich) (100 to 300 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: PC-3 cells (RRID:CVCL_0035; obtained from ATCC) were cultured in F-12 Kaighn’s Modification HAM medium (Sigma Aldrich) containing 10% heat-inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... round 12 mm glass coverslips were first coated with 50 μg/ml poly-l-lysine (Sigma-Aldrich, USA) diluted in water for 20 min at RT followed by washing with PBS and fixation with 0.5 % (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: NIH3T3 and MCF10A cells were starved in a medium consisting of Ham’s F-12 Nutrient Mixture (Sigma N4888) with 200 U/ml penicillin and 200 µg/ml streptomycin and 200 nM L-Glutamine and 0.5 % bovine serum albumin for the NIH3T3 cells and DMEM:F12 (Sigma D8437 ...
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bioRxiv - Developmental Biology 2022Quote: Mice were either dark- or light-adapted for 12 h before ERG recording and anesthetized with 2,2,2-tribromoethanol (Sigma). After the pupils of the mice were dilated by 0.5% tropicamide ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured onto 12mm glass coverslips in 12-well plates coated with poly-L-lysine (Sigma-Aldrich). Cells were fixed after 48h of transfection using 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2022Quote: ... Excess dye was removed by dialysis (Pur-A-Lyzer Mini Dialysis tubes, Sigma-Aldrich; MWCO 12-14 kDa) against PBS ...
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bioRxiv - Immunology 2022Quote: ... combined phorbol 12-myristate 13-acetate (PMA) and ionomycin (25 μg/ml and 32.5 μg/ml, respectively; Sigma) or medium alone ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then separated in 12% gel by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, ISEQ00010), which were then block with 5% milk in TBS-T buffer at room temperature for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... The J-Lat 6.3 cells were activated with phorbol 12-myristate 13-acetate (PMA, 16 nM, Sigma-Aldrich) and Ionomycin (0.5 μM ...
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bioRxiv - Neuroscience 2022Quote: CHO cells were cultured in 24-well-plates on poly-D-lysine coated glass coverslips in the growth medium (Dulbecco’s Modified Eagle’s Medium / Nutrient Mixture F-12 HAM (DMEM/F12, D8437; Sigma) supplemented with 5% foetal bovine serum (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: The chemical (2S, 3S)-1,4-Bis-sulfanylbutane-2,3-diol (DTT, CAS No. 3483-12-3) was obtained from Sigma; it is a strong reductant with chemical formula of C4H10O2S2 and MW 154.25g/mol ...
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bioRxiv - Neuroscience 2020Quote: ... THP-1 cells were differentiated into macrophages with 25 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma) for 48 hrs ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated via gel electrophoresis on 12 % polyacrylamide gel and transferred to a PVDF membrane (Sigma Aldrich) using a semi-dry blotting system ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM levamisole) and incubated for 12-17 h in 0.34 mg/mL nitrotetrazolium blue chloride (Sigma Aldrich) in buffer C with levamisole in a humid environment ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were electrophoretically separated on 4–12% SDS-PAGE gels and transferred to a PVDF membrane (Millipore, MA). Membranes were subsequently blocked with blocking buffer (Sigma ...
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bioRxiv - Systems Biology 2021Quote: ... we plated 10k – 30k cells on 12 mm collagen-coated glass coverslips (rat tail collagen, Sigma, #122-20). Cells were plated the afternoon prior to imaging ...
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bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
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bioRxiv - Cell Biology 2022Quote: RPE-1 (human retinal epithelial) cells were grown in Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 Ham (DMEM-F12; Sigma) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... with the addition that individual wells in the 12-well plate were incubated with pluronic acid (Sigma; P2443) overnight prior to tissue seeding ...
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bioRxiv - Plant Biology 2021Quote: Equal amounts of total proteins were separated on 12% SDS-PAGE and transferred to PVDF membranes (Millipore, #IPVH00010). The concentration of proteins was determined by Bradford assay ...
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bioRxiv - Immunology 2021Quote: ... neutrophil stimulation was performed by adding 200 ng/mL of phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich) for 30 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... were cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s medium and Ham’s F-12 medium with 3.1g/L glucose and 1mM sodium pyruvate without phenol red (D2906, Sigma) supplemented with 1.5g/L sodium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 12-20 % SDS–PAGE and transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA). The membrane was blocked with TBS-T (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were resolved in 10 % to 12 % SDS-PAGE and transferred to Immobilon transfer membrane (EMD Millipore). Membranes were blocked in ChemiBlocker (EMD Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... L-DOPA was coadministered along with the peripheral DOPA decarboxylase inhibitor benserazide hydrochloride (Sigma-Aldrich, 12 mg/kg).
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bioRxiv - Biochemistry 2021Quote: ... About 20 μg protein was loaded in 12% SDS-PAGE and transferred to PVDF (0.22 μM) membrane (Millipore). Membranes were incubated with primary antibodies including anti-β-actin (1:10,000 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... THP-1 monocytes were incubated with 30 ng·mL-1 4α-phorbol 12-myristate 13-acetate – PMA (Sigma Aldrich) for 24 hours (39) ...
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bioRxiv - Neuroscience 2020Quote: ... were administered haloperidol (0.5 or 2 mg/kg/day; haloperidol; n=12/group: Sigma-Aldrich, Gillingham, Dorset, UK) or vehicle (β-hydroxypropylcyclodextrin ...
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bioRxiv - Microbiology 2022Quote: ... Virus was removed and 2 mL of overlay media (EMEM 1X Lonza 12-684F, 5 % FBS HyClone, 0.6 % purified Agar OXOID #LP0028, 0.12% NaHCO3 Sigma #S5761, 0.01% Dextran Sigma #D9885) was added ...
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bioRxiv - Cell Biology 2022Quote: ... 100% humidity) in 6-well plates with 20 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) at a volume of 2mL/well and cell density of 250,000 cells/mL ...
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bioRxiv - Cell Biology 2022Quote: ... THP-1 cells were differentiated to macrophages using 100 nM phorbol 12-myristate 13-acetate (PMA, Sigma P8139), the PMA was also included in downstream experimental design ...
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bioRxiv - Microbiology 2022Quote: ... equal amount of protein was resolved on 12% or 10% SDS-PAGE and transferred onto PVDF membranes (Millipore) by semi-dry immunoblotting method (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... at the age of 12 weeks were anesthetized with Avertin (Sigma-Aldrich, 0.4 g/kg body weight, i.p.). The heads of mice were fixed on a stereotaxic frame ...
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bioRxiv - Molecular Biology 2023Quote: ... fractions containing mIL-12 complexes were first desalted using a HiPrep™ 26/10 Desalting column (Sigma-Aldrich) equilibrated in HBS ...
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bioRxiv - Neuroscience 2022Quote: ... Twenty µg of protein samples were loaded and separated on 4–12% Criterion Bis-Tris Gels (Millipore, USA) electrophoresis and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Physiology 2022Quote: ... Fully differentiated adipocytes (12 days post differentiation induction) were maintained in DMEM supplemented with 10% FBS (Millipore Sigma) until two days before experimentation when cells were fed with 10% calf serum (Millipore Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... filtered (0.45 µm) and CDX cells were infected with 1 mL virus containing 12 µg/mL polybrene (Sigma), followed by selection with Puromycin (Merck ...
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bioRxiv - Immunology 2024Quote: ... anti-IL-12 (RyD Systems) in the presence of 0.25 μg/mL Brefeldin A and 0.0025 mM Monensin (SIGMA) or in combination with anti-MICa/b ...
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bioRxiv - Neuroscience 2024Quote: ... Absolute and relative activity for the full 24-hour recording and 12-hour periods were also calculated for six different frequency bands (i.e., SWA 0.5-5 Hz, Theta 5-9 Hz, Alpha 9-12 Hz, Sigma 12-16 Hz ...
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bioRxiv - Molecular Biology 2024Quote: RPE1 h-TERT cells were grown in high-glucose DMEM/F-12 medium with L-glutamine (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Sigma-Aldrich) ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Equal amounts of protein were electrophoresed on a 12% SDS– polyacrylamide gel and transferred to PVDF membranes (Millipore). Blots were blocked for 1 hour at RT (room temperature ...
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bioRxiv - Immunology 2024Quote: ... 25 × 104 BMDMs were placed in 12-well plates and treated with 100 ng/mL LPS (Sigma–Aldrich) for 6 h ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... at 0.5 x 106 cells/well or on 12-mm glass coverslips coated with poly-D lysine (Millipore). Cultures were used for experiments at 14 DIV ...